349 resultados para terapia génica


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Ciência Odontólogica - FOA

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Pós-graduação em Ciência Odontólogica - FOA

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Pós-graduação em Ciências Biológicas (Genética) - IBB

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Introduction: Aortic insufficiency (AoI), a volume overload, is characterized by the diastolic reflux of blood from the regurgitating aorta to the left ventricle. This effect results from malfunctioning aortic cusps. The main cause of AoI in developing countries is rheumatic fever, including Brazil, and valvar degeneration in developed countries. There is a strong association between cardiovascular diseases and depression. Selective serotonin reuptake inhibitors (SSRI) are one of the most prescribed antidepressants in the world. Previous studies of our laboratory showed that the utilization of a SSRI, paroxetine, improved cardiac function in rats with sub-chronic AoI and reduced the daily ingestion of hypertonic sodium (NaCl 0,3M). Cardiovascular diseases can determine behavior changes like increase of anxiety, and it is yet unknown if AoI would determine anxiety or anhedonia, incapacity of obtaining pleasure through physical or sensorial experiences. A possible target for SSRI action could be a change in the expression of enzyme isoforms that collaborate in the contractile function of the heart muscle, like the heavy chains of myosine, the sarcoplasmatic reticulum Ca2+/ATPase (SERCA) and its regulator protein, phospholamban (PLB). Objectives: Evaluation of behavior parameters for anxiety and anhedonia state and genic expression of a-myosine, b-myosine, SERCA2a and PLB in the heart tissue of rats with subchronic AoI that received treatment with an SSRI (paroxetine) for 4 weeks. Methods: Surgery to induce AoI was performed on male Wistar rats, anxiety was evaluated by the elevated plus-maze (EPM) and state of anhedonia was tested by ingestion of 2% sucrose solution. After euthanasia the heart tissue was collected and total RNA was extracted to be analyzed by the RT-qPCR method. Results: Heart fractional shortening was preserved in rats with AoI that were treated compared to rats with AoI that were not treated. There was no statistically ...

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Radiopharmaceuticals are substances marked with radionuclides that can be used for detection and treatment of cancer, infections and inflammatory diseases. They emit several types of radiation through different decay routes, each radioisotope with its specific properties and uses. They can usually be produced from several different materials, by bombardment with particle beams in a nuclear research reactor or cyclotron, depending on their characteristics. Brazil has four public institutions which produce - or import - and distribute radiopharmaceuticals to hospitals and clinics throughout its territory. The largest such institution, Ipen, distributes 97% of radiopharmaceuticals used in the country. Some radiopharmaceuticals decay very quickly, meaning they must be produced and quickly administered to the patient in the same location, presenting a logistical challenge. Nuclear medicine in Brazil is a promising field and has been steadily growing, although rigid laws and a lack of qualified work force hinder Research and Development efforts for new radiopharmaceuticals. The construction of a new nuclear research reactor, in 2016, should generate self-sufficiency and economy in radiopharmaceutical production and avoid a future crisis in the supply of technetium-99m, the most important radioisotope, used in over 80% of procedures with radiopharmaceuticals.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)