Avaliação laboratorial da cinomose canina: estudo retrospectivo de 25 casos no município de Araçatuba-SP

Canine Distemper is a contagious, severe and multisystemic disease caused by a virus from Morbillivírus genus. The virus is distributed worldwide and it presents a high lethality rate, affecting mainly dogs. The diagnosis is based on clinical signs associated with hematological fi ndings. The observation of Lentz bodies in erythrocytes and leukocytes is the defi nitive diagnosis for the disease. The aim of this work was to test the hypothesis that the hematological profi le in dogs positive for canine distemper differs according to blood cell type presenting Lentz bodies. For this purpose, 25 dogs positive for the disease were evaluated at the Veterinary Hospital “Luís Quintiliano de Oliveira” UNESP, Araçatuba city. The diagnosis was based on the observation of Lentz bodies in blood smears. Fromthe total, 64% of dogs presented anemia, 16% leucopenia and 12% leukocytosis. Lymphopenia occurred in 76% of dogs. Viral inclusions were observed solely in neutrophils (32%), lymphocytes (28%) and erythrocytes (12%). Concomitant observation occurred in lymphocytes and erythrocytes (4 %), in lymphocytes and neutrophils (12%), in neutrophils and monocytes (4%) and in neutrophils and erythrocytes (4%). In an isolated case Lentz bodies were observed simultaneously in neuthrophils, monocytes and lymphocytes. In conclusion, hematological profi le is not associated with the presence of viral inclusion in a particular cell type.

Erliquiose canina: revisão de literatura

The hemoparasitosis ehrlichiosis is a disease with high incidence in veterinary clinic routine, which causes damage to animal and human health, thus requiring the rapid and accurate diagnosis for treatment witch consists of specific drugs in particular doxycycline. It is necessary to make a good prevention, to prevent the disease, especially with regard to the control of ticks, since this is the vector of the agent. This review aims to elucidate the details for the canine ehrlichiosis, as a tool to help veterinarians to better understanding of this disease.

Polimosfismo genético da glicose-6-fosfato desidrogenase na população da região de Araraquara-SP

The most important role played by the enzyme Glucose- 6-Phosphate Dehydrogenase (G6PD) in erythrocyte metabolism is in generating energy and reducing power used to protect the cell against oxidative attack. G6PD deficiency is the erythroenzymopathy that most frequently causes hemolytic anemia, and more than 130 molecular variants have already been identified. The aim of this study was to analyze the genetic mutations in the G6PD-deficient adult males in the population of the region of Araraquara, São Paulo State. Out of 5087 male blood donors, 89 were deficient for G6PD, as confirmed by assaying the enzyme activity and electrophoresis on cellulose acetate. Thus, a frequency of 1.75% of G6PD-deficient patients was found, this value being similar to other investigations in São Paulo state. Molecular analysis was performed by amplification of genomic DNA with specific primers and digestion with restriction enzymes. In 96.6% of the patients, the G6PD A¯ variant was observed, with mutations at residues 376(A→G) and 202(G→A). Mean G6PD specific activity among the patients was 1.31 IU.g Hb-1.min-1 at 37ºC, that is 10.8% of the normal activity of the G6PD B enzyme. The variant forms G6PD A¯ 680(G→T) and 968(T→C) were not found. In 3.4% of the deficient individuals, the G6PD Mediterranean variant was found, with a mutation at 563(C→T). In these cases, mean enzymatic activity was 0.25 IU.g Hb-1.min-1 at 37ºC, or 2.1% of the enzymatic activity of G6PD B. The use of traditional techniques, allied to the identification of the different molecular variants, is important for the understanding of the structural and functional properties and hemolytic behavior of the red blood cells of the patient.

Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil

The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florianópolis. (AU).

Avaliação radiográfica do sucesso e da qualidade do tratamento endodôntico em uma população brasileira

Objetivo: esta investigação tem como objetivo avaliar a prevalência de periodontite apical detectada na radiografia periapical e a relação entre a doença e a qualidade da obturação de canais radiculares realizada por estudantes de graduação. Métodos: foi investigada uma amostra aleatória de 131 prontuários de pacientes que haviam recebido tratamento do canal radicular na Faculdade de Odontologia de Araçatuba, Universidade Estadual Paulista. Realizou-se anamnese, radiografias e exame clínico quanto à sintomatologia dolorosa, presença e qualidade da restauração, bolsa periodontal, mobilidade dentária e trauma oclusal. Resultados: a porcentagem de sucesso obtido com o tratamento realizado pelos estudantes de graduação foi de 87,7%. Quando o tratamento foi adequado (43 casos), o percentual de sucesso foi de 90,69% (39 casos), enquanto para os tratamentos inadequados (14 casos), o percentual de sucesso foi de 78,57% (11 casos), sem diferença estatisticamente significativa (p>0,05). Não foi observada diferença estatisticamente significativa na taxa de sucesso relacionada ao sexo (p>0,05). A condição pulpar também não interferiu na taxa de sucesso (p>0,05). Conclusão: concluiu-se que o tratamento endodôntico foi satisfatório e alcançou uma taxa de sucesso adequada, em torno de 90% dos casos.

Uso da terra e clima urbano e suas influências sobre a população de Euglossini na cidade de Parnaíba, PI, Brasil

Pós-graduação em Geografia - IGCE

Expressão gênica e proteica de APC, E-caderina, β-catenina e Caveolina-1 no processo carcinogênico da próstata canina e suas metástases

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estrutura da população e epidemiologia de Moniliophthora roreri no Magdalena Medio Colombiano

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Trabalho multiprofissional: necessidades reais e as políticas públicas na atenção da população idosa

Concomitant with the aging process there is an increasing of incidence of chronic diseases. So, it emerges the need for adaptation in the health system that requires new skills of healthcare professionals in order to serve this population. Therefore, it is necessary to understand the aging process and establish preventive measures. These issues are still far from achieving the ideal, however, many government programs and universities have sought to improve themselves and adapt to this change through a plurality of actions and preventive or health promotion intervention which provide a more resolute and humanized care plan through a multidisciplinary approach.

Estrutura genômica de uma população de suínos base Landrace

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Onicodistrofia lupoide simétrica canina: relato de caso

Symmetric lupoid onychodystrophy, or lupoid onychitis, is a multifactor immune-mediated onychopathy. The aim of the present study is to report the occurrence of lupoid onychitis in a two year-old mongrel dog helped at the Small Animal Dermatology Service of Sao Paulo State University Veterinary Hospital, in Botucatu, São Paulo. The animal presented lameness, onycholysis in five digits, paronychia, onychomadesis, nail softening and leuchonychia, as well as melicerous crusts in interdigital spaces and nailbeds. No non-dermatological systemic symptoms were observed. Complementary exams were negative for parasites or fungi. After 15 days of treatment there was partial recovery followed by recurrence. Onychectomy was performed in two digits for histopathological examination and fungal culture, which was negative. Histopathology was consistent with lupoid onychitis, reinforcing the importance of this complementary exam.

Espaçamento e população de plantas na cultura da mamona na safra e safrinha: estudo econômico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comportamento do maracuajazeiro amarelo, variedade Afruvec, ante uma população de Fusarium solani, agente causal da podridão do colo

The objective of the present work was to verify the behavior of yellow passion fruit, Afruvec variety, in relation to a population of Fusarium solani, obtained from this crop. The experimental delineation was random blocks, containing 10 treatments [9 isolates and 1 control treatment], with 4 repetitions, each plot being represented by a vase containing a plant. A disk of culture medium colonized by each isolate of the fungus was inoculated in the wounded collar region of the plants of the Afruvec variety two months after sowing. The appraised parameters were: the pathogenicity, the incidence (number of dead plants) and the severity of the disease (length of the lesion in the collar region), until 60 days after inoculation. The Afruvec variety was susceptible to the fungus and also presented variability as to the severity of the disease and incidence in relation to the different isolates. The population of the fungus showed variability in regard to aggressiveness. In light of the evidence of genetic diversity in the F. solani population, it is also suggested, in the tests of selection of materials to the pathogen, that these materials should be evaluated in different places with a history of the disease or inoculation with a pool of isolates of the fungus, in order to know the wide resistance of the genotype to the pathogen.

Estudo do peso metabólico e índice de Kleiber na estimação de parâmetros genéticos de características ponderais em uma população de bovinos de raça brahman

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)