Cymbopogon martinii essential oil and geraniol at noncytotoxic concentrations exerted immunomodulatory/anti-inflammatory effects in human monocytes

ObjectivesIn traditional medicine, plants have formed the basis of sophisticated systems that have been in existence for thousands of years and still provide mankind with new remedies. Cymbopogon martinii, known as palmarosa, has been used in aromatherapy as a skin tonic due to its antimicrobial properties. It has also used in Ayurvedic medicine for skin problems and to relieve nerve pain. The immunomodulatory action of C.martinii essential oil (EO) and geraniol was evaluated regarding the production of pro- and anti-inflammatory cytokines (tumour necrosis factor (TNF)- and IL-10, respectively) by human monocytes in vitro.MethodsMonocyte cultures were incubated with EO or geraniol. After 18h, cytotoxicity assays were performed using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide method, and cytokine production was determined by ELISA.Key findingsThe variables showed no cytotoxic effects on monocytes. TNF- production was not affected by C.martinii and geraniol, and only the concentration of 5g/ml of C.martinii stimulated its production. On the other hand, all concentrations of C.martinii and geraniol increased IL-10 production by human monocytes.ConclusionsData showed that noncytotoxic concentrations of EO and geraniol exerted an anti-inflammatory action by increasing IL-10 production; moreover, geraniol seemed to be probably responsible for EO immunomodulatory activity in our assay condition.

Tumescent local anesthesia with ropivacaine in different concentrations in bitches undergoing mastectomy: plasma concentration and post-operative analgesia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Optimizing xanthophyll concentrations in diets to obtain well-pigmented yolks

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Daily photosynthesis, water relations, and ion concentrations of uuonymus irrigated with treated wastewater

Euonymus japonica Thunb. (euonymus) plants were submitted for 9 months to two irrigation treatments using water from different sources: a control (C) water with electrical conductivity (EC) less than 1.2 dS.m(-1) and reclaimed wastewater (RW) with EC approximate to 4 dS.m(-1). At the end of the experiment, no differences in the total dry weight were observed between treatments, whereas the leaf dry mass increased (to the detriment of the root part in RW plants). Throughout the day, the stem water potential (Psi(stem)) of the RW plants was lower than in C, whereas stomatal conductance (g(S)) was slightly reduced in RW from 0800 HR to 1200 HR, but no significant variation in photosynthesis (P-n) or energy conversion efficiency (F'(v)/F'(m)) in photosystem II was detected through the effect of salinity. Gas exchange and fluorescence showed a tendency to increase after midday in plants treated with RW. The photosynthetic behavior and fluorescence of RW plants may have been related to the nitrogen and chlorophyll content of the leaves, confirming the resistance of the photosynthetic mechanism to salinity in this species in these conditions. The toxic effects produced by high concentrations of boron (B), sodium (Na+) and chloride (Cl-) were offset by the effect of other ions like magnesium (Mg2+), potassium (K+), and phosphorus (P) in plants irrigated with RW, thus improving their physiological status without decreasing their ornamental value.

Lethal effect of high concentrations of Parecoxib and Flunixin meglumine on the in vitro culture of bovine embryos

Since cyclooxygenase (COX) inhibitors have been pointed out as potential treatments to increase pregnancy rates after embryo transfer, the present experiment aimed to evaluate the effects of flunixin meglumine (FM) and parecoxib (P), a COX-1 and 2 or COX-2 specific inhibitor, respectively, on the development of bovine embryos until the hatched blastocyst stage. In vitro produced bovine embryos were cultured in media with different concentrations of FM (0.14; 1.4; 14; 140 or 1400 mu g/ml) or P (0.09; 0.9; 9; 90 or 900 mu g/ml) and the production rates were evaluated. Concentrations of FM <= 14 mu g/ml and P <= 90 mu g/ml did not impair embryo development, although compiled data from non-lethal FM concentrations (<= 14 mu g/ml) indicated a toxic effect enough to decrease the hatching rate of blastocysts. Concentrations of FM at 140 and 1400 mu g/ml and P at 900 mu g/ml were lethal as no cleavage was detected on presumptive zygotes.

Concentrações séricas e peritoneal de proteínas de fase aguda em equinos submetidos à endotoxemia experimental, tratados ou não com lidocaína

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparative Studies of Bacillus thuringiensis var. israelensis Metabolism in Different Concentrations of Cassava Flour Processing Waste Based Media

Techniques of production of enthomopatogenic bacteria are developed aiming to increase the productivity and to reduce the costs of the fermentative process. Like this, it has been using agroindustrial wastes or by-products as nutrient sources in culture medium, having been used, in this study, the manipueira, a by-product of the processing of the cassava flour. Fermentations were performed in flasks of Erlenmeyer of 500 mL containing 250 mL of culture media, conditioned in shaker at 180 r.p.m. and 28°C, and the media were composed by manipueira, in concentrations that varied between 400 and 1000 mL/L. The time of the process varied between 48 and 120 hours. They appraised the following parameters: cellular growth, the production of spores, the reduction of organic matter (COD analysis) and the variation of reduction sugar. Although there was a proportional cellular growth to the manipueira concentration, the production of spores was similar in all the cases, at the end of the process, in spite of the smallest speed of production of the same ones in the highest concentrations. In relation to the variation of COD, it has, also, a percentile minor of reduction in the highest concentrations. In the analysis of variation of reduction sugars, the higher concentrations are the ones that they present larger slowness in the reduction of this.

Effect of diets with varying starch content on muscle glycogen concentrations during training and replenishment after highintensity exercise

Pós-graduação em Zootecnia - FCAV

Gabaergic and opioid receptors mediate the facilitation of NaCl intake induced by α₂-adrenergic activation in the lateral parabrachial nucleus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of training status and eNOS haplotypes on plasma nitrite concentrations in normotensive older adults: a hypothesis-generating study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of prey concentrations and feed training on production of Hoplias lacerdae juvenile

The aim of this work was to investigate the s of daily prey concentration during the first 15 days of active feeding of Hoplias lacerdae larvae, and the juvenile size on the feed training. In the first phase, the larvae received five Artemia nauplii concentrations (P). In the second phase, the juveniles from each treatment were trained to accept formulated diet. Superior growth was related to higher initial daily prey concentrations (900 and 1100 nauplii larvae-1). During feed training, the growth tendency was similar to that verified in the first phase. The lowest values of specific growth rate (SGR) were registered after the introduction of the semi-moist diet used in the feed training. However, the values of SGR recovered along the experiment and similar rates were found among the treatments. Survival, mortality and cannibalism were similar in the different treatments at the end of both phases. It can be concluded that: the prey concentration affects growth of H. lacerdae during the first 15 days of active feeding, and feed training can be initialized with juveniles of about 16 mm of total length.

Diversidade e evolução da microbiota lática autóctone em queijo Muçarela de búfala e aplicação tecnológica dos isolados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Gabaergic and opioid receptors mediate the facilitation of NaCl intake induced by alpha(2)-adrenergic activation in the lateral parabrachial nucleus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphological effects of neem (Azadirachta indica A. Juss) seed oil with known azadirachtin concentrations on the oocytes of semi-engorged Rhipicephalus sanguineus ticks (Acari: Ixodidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Physicothermal properties of aqueous sodium chloride solutions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)