343 resultados para GENERACIÓN DE PROTEÍNA MICROBIANA


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A presente patente diz respeito a um dispositivo de refrigeração de fluidos de corte interligado em máquinas de usinagem, retificadoras e/ou de corte, visando reduzir a contaminação microbiana dos fluidos de corte, oferecendo, paralelamente, maior poder de refrigeração sobre a peça e a ferramenta de corte, composto por caixa de resfriamento (2) com serpentina em banho de gelo ou um refrigerador de líquidos (2A), depósito do fluido (3), sendo que a caixa de resfriamento e o depósito de fluido são revestidos com material isolante térmico (4) e devidamente interligados por tubos (5).

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A presente patente diz respeito a um dispositivo usado para reduzir a contaminação microbian dos fluidos de corte através de raios ultravioletas, o qual c nsiste em um tampo metálico que se adapta ao reservatório (3) de fluido de corte fechando completamente sua face superior, cujo tampo (1) contém lâmpadas (2) ultravioletas germicidas (UV-C), dispostas lado a lado, apresentando, ainda, pequenas janel s (4) para observação dispostas lateralmente e interruptor (5) ara as lâmpadas (2), sendo que os reatores das lâmpadas (2) s o instalados sobre uma chapa metálica (6).

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504 (five hundred four) 1 day old male chicks from a commercial broiler line (Ag Ross 308) were used, distributed in 12 treatments, with 6 replicates per treatment. The experimental design was casually blocked and treatments were organized in a 2x2x3 factorial arrangement: two available phosphorus levels (0.45 and 0.34%), two phytase inclusion levels (0 and 1200 FTU/kg) and three crude protein levels (22.5; 20.5 and 18.5%). Tibia calcium percentage was influenced by protein and phosphorus levels in the diet, when using phytase. Tibia ash levels showed a linear effect when enzymes were added and showed a quadractic effect without it, and the higher value was observed with 22.5% crude protein in the diet. With higher phosphorus levels a linear effect was observed between crude protein in the diet and tibia ash weights, as a higher protein level had lower tíbia ash weight.

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The experiment was conducted to evaluate the ingestive behavior of feedlot lambs fed with an exclusive diet of concentrate with different percentages of protein (14, 16, 18, and 20%). A total of twenty four Santa Inês crossbred lambs, not castrated, with approximately 180 days of age, average live weight of 25kg, confined, in a completely randomized design, with four treatments and six repetitions. The time spent on feeding decreased linearly in 0.20 hours/day for each 1% increasing in protein percentage in the diet, the idleness increased linearly in 0.25 hours/day, and the total chewing decreased linearly in 0.25 hours/day. The increase in the protein percentage in the diet increased the feed efficiency linearly (kg DM and NDF/hour) in 0.038 and 0.005kg/hour, respectively, for DM and NDF fractions. However, the intake of dry matter (DM) and neutral detergent fiber (NDF), the rumination efficiency (kg DM and NDF/hour), as well as the period (nº /day) and the time (min) spent on feeding, ruminating and idling were not influenced by different protein percentages in the diet.

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O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+) e inserido em células de Escherichia coli BL21 (DE3). A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG), visualizada em SDS-PAGE e detectada por Western blot. Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Biotecnologia - IQ

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Pós-graduação em Zootecnia - FCAV

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The oral cancer model in hamsters shows many simílarities with developmental oral cancer in humans. The proliferating capacity is one the most characteristics of neoplásica ce/Is and detection of these ce/Is allow us, throughout of its counting, to achieve an estimated tumour growing index, with a consequent repercussion about prognostic and in the treatment of those lesions. 40 golden Syrian hamsters, both genders, aged between two to six mouth and weight 150g in average were used. The left síde of tangue of each animal was painted for eight consecutive weeks, with a solution of DMBA. Tongues were removed, fixed in 10% buffered fonnal solution. The histological slides were stained by AgNOR technique and by PCNA and β-catenin immunohistochemical antibodies. Statistical analyzes were performed by ANOVA one-way test and Tukey test. We may conclude that an association between AgNOR and PCNA might indicate the higher proliferating activity of the analyzed celIs. The experimental carcinogenesis model in hamster tongue is an available methodology for immunohistochemistry study. And finally, PCNA and β-catenin immunohistochemical antibodies may be used to analyze possible premalignant areas in oral leukoplakia

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)