Produção, imobilização e aplicação de lipase de Fusarium verticillioides

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Renewable nanocomposite layer-by-layer assembled catalytic interfaces for biosensing applications

A novel, easily renewable nanocomposite interface based on layer-by-layer (LbL) assembled cationic/anionic layers of carbon nanotubes customized with biopolymers is reported. A simple approach is proposed to fabricate a nanoscale structure composed of alternating layers of oxidized multiwalled carbon nanotubes upon which is immobilized either the cationic enzyme organophosphorus hydrolase (OPH; MWNT−OPH) or the anionic DNA (MWNT−DNA). The presence of carbon nanotubes with large surface area, high aspect ratio and excellent conductivity provides reliable immobilization of enzyme at the interface and promotes better electron transfer rates. The oxidized MWNTs were characterized by thermogravimetric analysis and Raman spectroscopy. Fourier transform infrared spectroscopy showed the surface functionalization of the MWNTs and successful immobilization of OPH on the MWNTs. Scanning electron microscopy images revealed that MWNTs were shortened during sonication and that LbL of the MWNT/biopolymer conjugates resulted in a continuous surface with a layered structure. The catalytic activity of the biopolymer layers was characterized using absorption spectroscopy and electrochemical analysis. Experimental results show that this approach yields an easily fabricated catalytic multilayer with well-defined structures and properties for biosensing applications whose interface can be reactivated via a simple procedure. In addition, this approach results in a biosensor with excellent sensitivity, a reliable calibration profile, and stable electrochemical response.

Cr+3 distribution in Al1 and Al2 Sites of alexandrite (BeAl2O4: Cr3+) induced by annealing, investigated by optical spectroscopy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influências naturais e antrópicas na qualidade da água subterrânea de poços de abastecimento público na área urbana de Marília (SP)

This work evaluated the natural and anthropogenic influences on the quality of groundwater from public supply wells in the urban area of Marília, São Paulo State. Sixteen sampling points were established in the Adamantina Aquifer, Bauru Aquifer System, analyzing the following parameters: electrical conductivity, temperature, pH, total suspended solids, HCO3 - , PO4 3-, SO4 2-, Cl- , F- , N-NO3 - , Ca2+, Na+ , K+ , Mg2+, Si4+, Fe3+ and Al3+. The results indicated that the groundwater in the urban area of Marília has a slightly acid pH and low conductivity, with the ionic composition presenting a low cation and anion concentration and is classified as soft water and calciumbicarbonated water. Natural sources of elements/compounds can be attributed to the dissolution of carbonates during the water/rock interaction, controlling pH, alkalinity and electrical conductivity, and hydrolysis of other mineral constituents of sedimentary rocks from Adamantina Formation, with the exception of quartz. High concentrations of N-NO3 - found in some public supply wells in urban Marilia were due to sewage.

Effects of Protein A in Detection of Canine Distemper Virus through immunosensor construction

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da promiscuidade catalítica de soroalbuminas em sínteses orgânicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Purificação parcial e caracterização bioquímica de uma isoforma de β-glicosidase do fungo termofílico Myceliophthora thermophila M.7.7

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Evaluation of pure and Ag-doped TiO2 films in the photocatalytic activity of phenol

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nanopartículas magnéticas como suporte para imobilização de lipases

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção do peptídeo p17 (HIV) baseado em SERS (Surface-enhanced Raman Spectrosocpy)

Pós-graduação em Química - IQ

Avaliação da cinetica bacteriana na biolixiviação de calcopirita

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)