Ocorrência de anticorpos contra Leishmania spp., Neospora caninum e Toxoplasma gondii em soros de cães atendidos no Hospital Veterinário da Universidade Estadual de Londrina-Pr

The aim of this study was to detect the presence of IgG antibodies anti-Leishmania spp., Toxoplasma gondii and Neospora caninum in dogs from a Veterinary Hospital from Universidade Estadual de Londrina. Blood samples from 112 animals were obtained by jugular venipuncture to obtain sera. The samples were tested by indirect immunofluorescence to detect antibodies anti-Leishmania spp., anti-N. caninum and anti-T. gondii. Thirteen (11.61%), 25 (22.32%), and 57 (50.89%) samples were positive for Leishmania spp., N. caninum, and T. gondii, respectively. The co-presence of anti-Leishmania spp. and N. caninum was observed in 6 (5.36%), anti-Leishmania spp. and anti-T. gondii in 8 (14.7%), and anti-N. caninum and anti-T. gondii in 18 (16.07%) samples. The co-presence of anti-Leishmania spp., anti-N. caninum and anti-T. gondii was observed in 5 (4.46%) dogs. There was a higher prevalence of Leishmania in Toxoplasma and Neospora positive animals, however, these results were not statistically significant (range p = 0.052 p = 0.06). The dogs have an important role in the epidemiological cycle of these diseases, which are important in animal and public health. The northern state of Paraná is an endemic area for human cutaneous leishmaniasis, therefore, studies should be conducted to uncover the real role of dogs as reservoirs of Leishmania to humans in the state.

Mume clonal rootstocks for 'Aurora-1' peach in São Paulo State, Brazil

In spite of significant results achieved with scion genetic improvement in stone fruits, the peach culture in Brazil still needs studies and new technologies regarding the use of rootstocks. A wide research project has being developed at the Faculdade de Ciências Agrárias e Veterinárias (FCAV/UNESP), Jaboticabal, Brazil, dealing with the use of mume clones (Prunus mume) as rootstocks for peach trees, which has produced promising results. In this research, two mume genotypes propagated by herbaceous cuttings were tested as rootstocks for peach cultivar Aurora-1. Three different tree spacing were used: 6 x 2 m, 6 x 3 m and 6 x 4 m. The experiment was carried out at Vista Alegre do Alto (21°10'14 S, 48°37'45 W, 700 m of altitude), São Paulo State, Brazil. Growing field conditions included Hapludalfs soil with medium sandy texture and using micro sprinkler irrigation. The region has an average chilling accumulation 17.9 hours per year. The evaluations were taken in 2005 and 2006 (2nd and 3rd year after planting, respectively). The trunk diameter was evaluated every three months, from the 24th to the 41st month after planting, totalizing seven evaluations. Plants on 'Rigitano' had higher trunk diameter on the 33rd, 39th and 41st month after planting (May/06, November/06 and February/07, respectively). No significant differences were observed in the other evaluations. The diameter at 5 cm above to the graft point was larger than below, but no incompatibility symptoms were observed between rootstocks and scion. Spacing tested did not influence trunk diameter, phenology and flower bud production in 'Aurora-1' scion. In conclusion, 'Rigitano' and 'Clone 15' are recommended for high density plantings of peach 'Aurora-1' in Brazil, and the 6 x 2 m spacing can be recommended, with productivity advantages for peach under low air relative humidity and mild winter conditions. © ISHS 2012.

Tumor maligno da bainha de nervo periférico envolvendo raízes nervosas do terceiro segmento medular lombar em um cão

Malignant peripheral nerve sheath tumors (MPNST) involving spinal nerve roots are uncommon in dogs. A nine-year old, intact, mixed-breed dog, demonstrated clinical signs of incoordination in the pelvic limbs and micturition for approximately one week. Clinical examination revealed proprioceptive deficits and bilateral patellar hyperreflexia. During exploratory celiotomy a mass was observed adhered to the lumbar vertebral segments. Medical therapy was initiated, but neurological signs were progressive, and the owner opted for euthanasia. Gross examination showed that the mass in the abdominal cavity was attached to the lumbar segments L3 and L4, causing bone lysis in L3, but showed no tumor invasion into the spinal canal. Microscopic features were characterized by prominent proliferation of ovoid and fusiform cells with poorly defined cytoplasm arranged in interlacing bundles and concentric whorls. The cells were embedded in a delicate to moderate collagenous stroma and moderate anisokariose and high mitotic activity were noted. The immunohistochemical assay showed positive staining for GFAP, S-100 protein and vimentin, and negative staining for factor VIII, α-actin and citokeratine. The definitive diagnosis of malignant peripheral nerve sheath tumor was made on the basis of the histological and immunohistochemical findings.

Comportamento ingestivo de novilhos de corte sob diferentes alturas de pastejo do capim Tanzânia1

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Viabilidade econômica de quatro intensidades de pastejo do capim Tanzânia na pecuária de corte1

Were evaluated the economic effects of four grazing heights (20, 40, 60 and 80 cm) of Tanzânia grass for beef cattle. The total area was 12 acres divided into paddocks of one hectare, with three replications. We used three animals, males, Nelore per paddock, as more animals need additional adjustment to the height you want. The reduction in sward height allowed higher stocking rate, which, even with a reduction in individual performance, there was more beef production per area. The interest rate on capital given the property represented the largest share in the final cost of production. The participation of fixed variables showed a positive linear function of the increase in height from grazing, showing significant reduction of the production scale. A reduction in cost of production per hectare per year with increasing grazing height. No differences were found in certain economic indicators, and the four systems remunerate the capital invested.

Composição química e degradabilidade ruminal de silagens da cana-de-açúcar tratada com aditivos químicos e bacteriano1

The objective of this trial was to evaluate the effect of the addition of chemical and bacterial additive in the ensiling of sugar cane (Saccharum officinarum L.) on chemical composition, pH, kinectic fraction and in situ degradation of nutritions components of silages. Five rumen-cannulated 1/2 Simental + 1/2 Zebu steers were allotted to a completely randomized design. The steers were placed in individual cages and they were fed with diets with 76% forage (%DM). Five silages were evaluated: control - sugar cane, no additives; urea - sugar cane + 0.5% of urea (wet basis); inoculant - sugar cane inoculated with LactoSilo® (390 g/40 t forage); NaOH - sugar cane + 1.0% of sodium hydroxide (wet basis); CaOH - sugar cane + 0.6% of calcium hydroxide (wet basis). The silage additives with sodium hydroxide showed the highest pH values before (11.20) and after (4.87) for silage. No differences were observed among the silages for dry matter (26.85), crude protein (5.25) and acid detergent fiber (57.21). Fractionation of dry matter and organic matter of silages showed similar behavior, with higher values of the soluble fraction (fraction A) for silages with sodium hydroxide (45.86 and 30.95%) and calcium hydroxide (29.47 and 26.13%). The use of sodium hydroxide allowed obtaining higher values for the degradation of cell wall components of silages from cane sugar. The potencial and effective degradability with 3, 5 and 8%/h of passage rate were respectively 88.44, 64.45, 56.73 and 49.83% for NDF and 82.57, 55.51, 46.72 and 38.83% for ADF, indicating that the use of sodium hydroxide as chemical additives can improve the nutritive value of cane sugar silage.

Adubação de cobertura na batata-doce com doses combinadas de nitrogênio e potássio

Balanced fertilizations with N and K often increase the performance of crops, however, when there is absence of one of these nutrients in poor soils, can reduce crop response to fertilization with the other. The objective of this work was to evaluate the productivity of sweet-potato, the amount of sweetpotato without quality, and leaf nutrients analysis, of sweet-potato crop fertilized with N and K. The trial was conducted in farming for commercial production, in Presidente Prudente, São Paulo State, during February-June 2007, in a dystrophic Ultisol of medium texture. The experimental design was a randomized complete block design, with four replications, on factorial 4 x 4: levels of 0, 30, 60 and 120 kg N ha-1 (urea source) combined with levels of 0, 30, 60 and 120 kg K2O ha-1 (KCl source), applied to 39 days after planting of the crop. The sweet-potato is responsive to topdressing application with nitrogen and potassium, however, the greatest increases in productivity occur when doses of N and K are combined. Fertilization with N and K not increase the amount of sweet-potato without quality marketing. The highest increase in productivity of sweet-potato is reached with topdressing combined with 100 kg N ha-1 plus 120 kg K2O ha-1.

Exportação de nutrientes em plantas de jambu, sob diferentes adubações

The jambu is a broad vegetable consumption in Northern Brazil, especially in Pará, known by the jambu and other common names is native to the Amazon region has been used and cultivated for culinary and also recently in natural medicines by their chemical properties, attributed to the spilanthol compound. Knowing the amount of nutrient uptake in plants, especially at the taken, it is important to evaluate the removal of nutrients necessary for economic fertilizer recommendations. So the goal of this project was to determine the accumulation of nutrients in plants of jambu (leaf and inflorescence) under different fertilizations. The experiment was conducted at São Manuel Experimental Farm UNESP. The statistical was arranged in the randomized block design, in a 2 x 6 factorial scheme, two sources of fertilizers (organic and mineral) and six doses of nitrogen, with four replications. We evaluated the macronutrients of accumulation N, P, K, Ca, Mg, S and micronutrients of accumulation B, Cu, Fe and Zn in leaves and inflorescence. The plants responded more jambu nutrients of translocation phosphorus (P), magnesium (Mg), sulfhur (S), boron (B), copper (Cu) and iron (Fe) in the inflorescences and phosphorus (P), calcium (Ca), manganese (Mg), sulfur (S), boron (B), copper (Cu) and iron (Fe) in leaves to organic fertilization demonstrating the effectiveness of using this source of fertilizer nutrients indicating that this was a defining characteristic in response to the accumulation of nutrients in the leaves and inflorescences jambu. Plants jambu are more responsive to fertilizer for the mineral of translocation nitrogen (N) and manganese (Mn) for both the sheet and for the inflorescences of plants jambu.

Nitrogênio e idade de corte na qualidade da biomassa de capimelefante para fins agroenergéticos cultivado em Latossolo

Nowadays one of the major challenge for research is the production of alternative agrifuels energy material derived from plant biomass. Allied to this, the proper management of nitrogen, becomes fundamental for productivity gains and improvement in quality of elephant grass biomass. Thus, the objective was to evaluate the effect of nitrogen fertilization and plant age effect on the quality of elephant grass biomass for energy purposes. The experiment was performed with genotype Paraíso, grown in a dystrophic Oxisol in the southern state of Tocantins. The design was randomized blocks with subdivided plots, situating plots as nitrogen rates (0, 50, 100 and 150 kg ha-1) and the subplots cutting age (120, 150 and 180 days after planting ) four replicates. We evaluated the relationship carbon/nitrogen (C:N) and leaf/stem (L:S), levels of neutral detergent fiber (NDF) and ash contents in dry biomass produced. The application of nitrogen increased by 12% to C:N ratio of the plant. The advanced age of cut forage was increased 9% in the C:N plant, however, decreased by 12 and 14% levels of ash produced in the stem and leaves, respectively. The production of elephant grass for agrifuels is feasible with management system proposed, quality characteristics desirable, such as high C:N ratio, above 40% and higt fiber content, above 50%.

Primeiro relato de atividade patogênica de Hirsutella thompsonii (Fischer) sobre o ácaro-da-erinose-da-lichia Aceria litchii (Keifer)

The litchi erineum mite, Aceria litchii (Keifer) is found in all producing regions attacking leaves and flowers of litchi plants. The mite attack young leaves and causes the erinea on leaf surface, which later become brown galls with velvety appearance. Severe attacks can cause leaf drop and destruction of branches end directly production affecting. In 2009 year it was registered a heavy infestation of the pest on litchi plants (Litchi chinensis Sonn.) in the municipality of Casa Branca, São Paulo, Brazil (2127'O; 4702'S; 679 m altitude). In June, many galls caused by mite infestation showed a mycelium of white coloration and many eriophyid dead. The fungus was identified as Hirsutella thompsonii (Fischer). The results suggest that galls may facilitate the fungus development and its permanence on the plants. Thus, the possibility of mite biological control with H. thompsonii should be investigated.

Déficit hídrico na eficiência de herbicidas e nas características bioquímicas de Euphorbia heterophylla

The objective of this work was to evaluate conditions the effectiveness of acetolactate synthase (ALS) and protoporphyrinogen oxidase (PROTOX) inhibitors in the Bidens pilosa control under two water deficit conditions, as well as to determine the action under the content of soluble carbohydrates and protein and free amino acids of weed. The experimental design was randomized completely design, with four replications, with the treatments setup in a factorial scheme 4x2, with four herbicides (fomesafen lactofen, chlorimuron-ethyl and imazethapyr), and two soil water conditions (-0.5 MPa and -0.01MPa). At 7, 14, 21 and 28 days after application (DAA), was assessed visually control efficiency of herbicides. For the determination of organic solutes plants were collected at 24, 48, 72 and 96 hours after application (HAA), except for the amino acids were analyzed 48, 72 e 96 HAA. Herbicides fomesafen and lactofen were efficient to control E. heterophylla, while the ALS inhibitors (chlorimuron-ethyl e imazethapyr) provided an unsatisfactory control. Water deficit altered the efficiency of herbicides, mainly chlorimuronethyl. Lactofen provided a smaller content of soluble carbohydrates, in the same way, the protein ranged in the 72 HAA, the lower value observed for imazethapyr e lactofen respectively. Herbicide lactofen increased the concentration of free amino acids, while the imposition of water deficit caused an increase in soluble carbohydrate content.

Crescimento e acúmulo de nutrientes da cultura do quiabeiro

With the objective to quantify the okra crop's growth and macro and micronutrients accumulation during its life-cycle obtaining equations that best represent it, an experiment was carried out at Jaboticabal city, SP, Brazil, from January 14th to May 14th, 2009. The cv. Santa Cruz 47 was directly sowed into open soil furrows, in a spacing of 1 x 0,2 m. During the life-cycle were realized random samples of plants at 15 days intervals. The means were adjusted to polynomial regression equations set of non-linear parameters. After 50 days from sowing there has been a large increase of dry matter in plant' parts. At the final harvest, 120 days after sowing, the leaves, stems and fruits accounted for 21, 71 and 8%, respectively, from the total plant dry matter. The decreasing sequence of nutrient accumulation was K, Ca, N, Mg, P, Fe, B, Mn, Zn and Cu equivalent to 6,002.8; 4,733.8; 2,930.8; 1,196.3; 473.7; 436.2; 49.8; 10.4; 7.1; 7.1 e 1.5 mg plant-1. The quantity accumulated of culture were 146.5; 23.7; 300.1; 236.7; 59.8 e 21.8 kg ha-1 of N, P, K, Ca, Mg and S, respectively, and 520.0; 76.0; 2,491.0; 355.0 e 355.0 de B, Cu, Fe, Mn and Zn, respectively.

Caracterização imunoistoquímica de neoplasias de bexigas associadas à hematúria enzoótica bovina

Bovine enzootic hematuria is characterized by the development of hemangiomatous lesions from several types of neoplastic processes, from epithelial and mesenchymal origin. In this research the histogenesis of neoplastic lesions found in bladder of bovines with enzootic hematuria from Caparaó microregion in the state of Espírito Santo, Brazil was determined. To accomplish this objective, immunohistochemical analysis was performed with primary antibodies: anti-vimentin, anti-cytokeratin, anti-CD31 and anti-uroplakin. Neoplasms found included urothelial carcinoma, in situ carcinoma, adenocarcinoma, hemangioma, myxoma e hemangiosarcoma. Immunohistochemical staining of cytokeratin in epithelial neoplasms and vimentin in mesenchymal neoplasms was significant (p<0.05). CD31 was positive in all the vessels of all samples, however, the staining was significant (p<0.05) in the tumor endothelial cells of the vascular mesenchymal neoplasms, as in hemangiomas and hemangiosarcomas. Uroplakin III staining was uneven in several neoplastic types and showed no significant difference (p>0.05). Most neoplasms showed an atypical uroplakin staining on urothelium and, in the case of hemangiosarcomas there was no staining of the urothelium at all. The Spearman statistical analysis revealed a positive correlation (r= 0.63, p= 0.05) between CD31 and vimentin and between cytokeratin and uroplakin (rs= -0.61, p= 0.05). It was concluded that biomarkers anti-cytokeratin, anti-vimentin and anti-CD31 are important for the diagnosis of neoplasms epithelial, mesenchymal and vascular mesenchymal, respectively. It is possible to use vimentin and CD31 in association in vascular mesenchymal neoplasms and cytokeratin and uroplakin in epithelial neoplasms. The uroplakin is an effective marker, not only for tumor diagnosis, but also to evaluate the urothelial integrity.

Ameloblastoma mandibular rostral em um cão

Odontogenics tumors are uncommon in the dog. Ameloblastoma is considered a benign, aggressive and non-inductive odontogenic neoplasia commonly located in the incisor teeth. Its conclusive diagnosis is obtained by histopathological examination. Due to the expansive nature of this neoplasia, its resection needs to have an adequate surgical margin so it will have excellent prognostics. A canine, male, Pekingese, 8 years old had an increased volume in the rostral region of the mandible, adhered, consistency moderately firm. Radiography revealed extensive mandibular bone lysis caused by the neoplasia. Biopsy identified the tumor as ameloblastoma. Combining data from the clinical, radiographic and histopatological study and after explain to the owner regarding the postoperative esthetic it was held a bilateral rostral mandibulectomy. The fragment removed was assessed radiographically and histopathologically for evidence of tumor at its edges. The patient is active and healthy without evidence of metastasis or recurrence. The radiographic and histopathologic evaluation of edges of the fragment showed no tumor evidence. The patient is active and healthy without evidence of metastasis or recurrence since January 2010 when mandibulectomy was performed.

Influência de pontas de pulverização e adjuvantes no potencial de redução de deriva em túnel de vento

The drift is intimately linked to inappropriate use of pesticides and an important factor for reducing it, is the correct selection of spray nozzles and adjuvants. The objective of this work was to evaluate the drift potential in wind tunnel with different spray nozzles and different concentrations of adjuvants. The experiment was composed by six spray solutions (vegetable oil (in three concentrations), mineral oil, surfactant and reducing drift), which were applied with two nozzles, one pre-orifice flat fan (DG 8003 VS) and other with air induction (AI 8003 VS), totaling 12 treatments, with three repetitions. The equipment used was a wind tunnel, where the drift collections were made at different points. The treatments averages were compared using Confidence Interval at 5% probability. The analysis of the percentage of drift showed that the treatments had different behaviors. The nonylphenol ethoxylate adjuvant presented the highest drift when applied with the nozzle of pre-orifice and the lowest drift when applied with the air induction. The behavior of these nozzles when the oil-based adjuvant was used showed apposite results to those obtained for the surfactant. For the DG nozzles the lowest percentage of drift, at all analyzed distances, was observed to the treatment with vegetable oil (1.0%) and with the AI nozzles the lower drift was found for the treatment with nonylphenol ethoxylate (0.0625%), for the four distances in the collection. The result showed that both the spray nozzles and adjuvants alter directly the drift potential. There was no proportionately between the concentration of the oil-based adjuvant and the drift percentage.