Plasmodium falciparum: sequence diversity and antibody recognition of the Merozoite surface protein-2 (MSP-2) in Brazilian Amazonia

The merozoite surface protein-2 (MSP-2) of Plasmodium falciparum comprises repeats flanked by dimorphic domains defining the allelic families FC27 and IC1. Here, we examined sequence diversity at the msp-2 locus in Brazil and its impact on MSP-2 antibody recognition by local patients. Only 25 unique partial sequences of msp-2 were found in 61 isolates examined. The finding of identical msp-2 sequences in unrelated parasites, collected 6-13 years apart, suggests that no major directional selection is exerted by variant-specific immunity in this malaria-endemic area. To examine antibody cross-reactivity, recombinant polypeptides derived from locally prevalent and foreign MSP-2 variants were used in ELISA. Foreign IC1-type variants, such as 3D7 (currently tested for human vaccination), were less frequently recognized than FC27-type and local IC1-type variants. Antibodies discriminated between local and foreign IC1-type variants, but cross-recognized structurally different local IC1-type variants. The use of evolutionary models of MSP-2 is suggested to design vaccines that minimize differences between local parasites and vaccine antigens. (C) 2004 Elsevier B.V. All rights reserved.

Detection of Hepatozoon spp in naturally infected Brazilian dogs by polymerase chain reaction

Hepatozoon canis was molecularly identified in Rio de Janeiro State, Brazil. Twelve dogs from urban areas were studied by blood smear examination and the polymerase chain reaction (PCR) assay. From these dogs, only 1 was positive in both blood smears and PCR.

Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Brazilian canine hepatozoonosis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Prevalence of Hepatozoon spp. (Apicomplexa, Hepatozoidae) among recently captured Brazilian snakes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conquering Brazil: the invasion by the exotic gekkonid lizard Hemidactylus mabouia (Squamata) in Brazilian natural environments

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

New host records of Brazilian pentastomid species

Pentastomida é um táxon de organismos parasitas obrigatórios de sistema respiratório de vertebrados, principalmente répteis. Embora esse táxon seja muito importante para a compreensão da filogenia dos Metazoa, tem recebido pouca atenção. No Brasil, existem poucas coleções que abrigam espécies de pentastomídeos, quais sejam: a Coleção Helmintológica do Instituto Oswaldo Cruz (CHIOC), a Coleção de Invertebrados do Laboratório de Zoologia da Universidade Regional do Cariri (LAZ-URCA) e a Coleção Helmintológica do Laboratório de Parasitologia de Animais Silvestres (LAPAS). O presente trabalho descreve as espécies de pentastomídeos depositados na Coleção Helmintológia do LAPAS. O trato respiratório e as cavidades do corpo dos répteis foram removidos e analisados sob Microscópio Esteroscópico; quando encontrados os pentastomídeos, foram montados slides em meio Hoyer e identificados. Foram identificadas quatro espécies e outras três ficaram identificadas no nível de gênero, tendo sido registrados quatro novos hospedeiros para as espécies de pentastomídeos.

Population structure of the malaria vector Anopheles darlingi in Rondônia, Brazilian Amazon, based on mitochondrial DNA

Anopheles darlingi is the most important Brazilian malaria vector, with a widespread distribution in the Amazon forest. Effective strategies for vector control could be better developed through knowledge of its genetic structure and gene flow among populations, to assess the vector diversity and competence in transmitting Plasmodium. The aim of this study was to assess the genetic diversity of An. darlingi collected at four locations in Porto Velho, by sequencing a fragment of the ND4 mitochondrial gene. From 218 individual mosquitoes, we obtained 20 different haplotypes with a diversity index of 0.756, equivalent to that found in other neotropical anophelines. The analysis did not demonstrate significant population structure. However, haplotype diversity within some populations seems to be over-represented, suggesting the presence of sub-populations, but the presence of highly represented haplotypes complicates this analysis. There was no clear correlation among genetic and geographical distance and there were differences in relation to seasonality, which is important for malarial epidemiology.

A new species of the Rhinella margaritifera group (Anura: Bufonidae) from Brazilian Pantanal

We describe adult morphology, advertisement call and some natural history traits of a new species of toad from the Pantanal, western Brazil. Rhinella paraguayensis sp. nov. belongs to the Rhinella margaritifera group, and is characterized by medium size, snout rounded in dorsal view, with a vertical apical ridge, supraorbital crests weakly developed, parietal crest not well developed, postorbital crest prominent, presence of a dorsolateral line of tubercles, tympanum evident, bony protrusions at angle of jaws, absence of vertebral apophyses and of projections on upper eyelids, and parotoid glands small. The new species is distinguished from other species of the group by geographic distribution and by the use of floating mats of vegetation as reproductive site.

Genotyping of Brazilian Giardia duodenalis human axenic isolates

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

New record of Haplometroides intercaecalis (Digenea, Plagiorchiidae) infecting a Brazilian snake

Phalotris matogrossensis (Serpentes, Colubridae) was reported as a new host for Haplometroides intercaecalis ( Digenea, Plagiorchiidae). The host snake was obtained from the municipality of Anastacio, state of Mato Grosso do Sul, Brazil. One specimen of H. intercaecalis was recovered from the esophagus of the host and identified by the intercecal position of the vitellaria in the pre-acetabular region. This paper describes the second report of the occurrence of this trematode in fossorial snakes of the genus Phalotris in the state of Mato Grosso do Sul, Brazil.

New reports on parasitism by Haplometroides buccicola (Digenea, Plagiorchiidae) in Brazilian snakes

The occurrence of Haplometroides buccicola (Digenea, Plagiorchiidae) in the esophagus of two Brazilian snakes is reported in the present study. The trematodes were collected from one Micrurus corallinus (Elapidae) and one Phalotris lativittatus (Colubridae); both snakes were found in Botucatu city, São Paulo State, Brazil. Morphological and morphometric analyses of the trematodes are presented. For the first time Micrurus corallinus has been recorded as a host for H. buccicola and this is the second time that P. lativittatus has been reported as a host for this trematode species.

Bouchardia rosea, a vanishing brachiopod species of the Brazilian platform: taphonomy, historical ecology and conservation paleobiology

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Feeding behaviour and ecosystem role of the red mangrove crab Goniopsis cruentata (Latreille, 1803) (Decapoda, Grapsoidea) in a subtropical estuary on the brazilian coast

This study provides information concerning the feeding behaviour of Goniopsis cruentata (Latreille, 1803), through analysis of stomach contents according to demographic categories. Collections were carried out monthly from May 2005 through April 2006 in a subtropical estuary on the southeastern Brazilian coast (23 degrees 29'24 '' S 45 degrees 10'12 '' W). The crabs were collected by hand, with a 2-hour sampling effort by three people. In the laboratory, the crabs were sexed and measured for greatest carapace width, and grouped into demographic categories: adult males, juvenile males, adult females, juvenile females, and ovigerous females. For the fullness analysis, the stomachs were grouped into two categories: (1) E = Empty, with no food; and (2) F = Full, whether partially filled or totally. The frequency-of-occurrence method was used to characterize feeding behaviour, and the demographic categories recognired were compared. We obtained stomachs from 171 adult males, 69 juvenile males, 136 adult females, 72 juvenile females, and 41 ovigerous females, of which 85.6% were full. of the eight food items recorded, sediment was the most frequent, and unidentified material was the least. Goniopsis cruentata can be characterized as a generalist feeder, exploiting most of the food items available in the mangrove swamps. In spite of this generalist behaviour, the dominant presence of sediment suggests that G. cruentata is primarily a detritivore that exploits particulate organic matter from microbial biodegradation, one of the most important mangrove functions. The trophic role of this crab in the ecosystem showed no significant differences among the demographic categories, and seems to be wider than those observed for sesarmid and ocypodid mangrove crabs. These ecosystem engineers may occupy different positions in the trophic chains of estuarine environments.

Morphological and morphometric comparisons of the first zoea of four species of purse crabs (Decapoda: Brachyura: Leucosiidae) from the Brazilian South Atlantic

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)