Helmintofauna associada à Physalaemus cuvieri proveniente de duas áreas de Mata Atlântica

From November 2009 to January 2010 we collected 22 specimens of Physalaemus cuvieri from a preserved area (State Park of Serra do Mar - Núcleo Santa Virginia, São Paulo State, Brazil) and 21 specimens from a disturbed area with habitat fragmentation (rural areas – São Luis do Paraitinga municipality, São Paulo State, Brazil) to compare the helminth fauna associated to this anuro species. The helminth fauna is composed by the nematodes of family Cosmocercidae, Physaloptera sp. and Rhabdias sp. as well as Acanthocephalus saopaulensis in the individuals from the preserved area; the individuals from the fragmented area also presented nematodes of family Cosmocercidae, Physaloptera sp. and Rhabdias sp. as well as Oswaldocruzia subauricularis and the monogenea Polystoma cuvieri. Prevalence, mean intensity of infection, mean abundance, mean richness, importance index (IE) and Dominance frequency were calculated in both localities, but there was no statistical diference. The diversity of helminth species was calculated by Shannon, Brillouin and Berger-Parked index, and by using Shannon diversity t-test we compared helminths diversity associated with anfibian from this two localities

Helmintofauna de Hypsiboas raniceps (Cope, 1862) (Anura, Hylidae) no Pantanal Sul, Mato Grosso do Sul, Brasil

The helminth fauna of twenty-five Hypsiboas raniceps (Anura, Hylidae) specimens collected in the pantanal wetland, municipality of Corumbá, Mato Grosso do Sul State, Brazil was studied. Twenty (80%) specimens were parasitized with at least one helminth species. The helminth fauda is composed by the nematodes Oswaldocruzia mazzai, Cosmocerca podicipinus and Physalopteroides venancioi beyond the no identified species of the genus Rhabdias, Physaloptera and Raillietnema and the family Cosmocercidae. Cysts and larvae of nematodes were also found. Catadiscus propinquus and diplostomid metacercariae were the recovered trematodes

Atividade in vivo de parasporina extraída de B. thuringiensis sobre o câncer colorretal murino (CT‐26)

Bacillus thuringiensis (Bt), is an environmental Gram-positive spore-forming bacterium that produces crystalline parasporal protein (Cry) during sporulation. The inclusions often exhibit strong and specific insecticidal activity, making Bt an agent for agricultural controlling insects pest, mites, protozoa and nematodes. Recent studies reported that some of these Crys do not show cytotoxicity against insects but they are capable to kill some human and animal cancer cells. These proteins were denominated parasporins (PS). However, antitumor activity of Bt parasporin on the development of murine colorectal cancer (CT-26), are not well studies and these are no reports on the in vivo effect of these proteins. Thus, the present study evaluated the in vitro and in vivo anti-tumoral activity of Bt parasporin against the murine colorectal cancer line CT-26. Therefore, Balb/c mice were s.c. inoculated with CT-26 cells and weekly treated with parasporin (i.p.) pre-activated by enzymatic digestion with trypsin or proteinase K. Our results have shown, for the first time, that despite the anti-tumor activity in vitro, parasporin crystals couldn’t combat tumor growth in vivo. Instead, this protein was highly toxic, affecting the liver and spleen, with possible effect on other organs, decreasing the survival of treated animals. The results indicate the need for studies to better detoxification or manipulation of parasporin for therapeutic use and new studies for analysis of toxicological effects of repetitive exposure of farmers to this toxin

Controle genético da resistência a Meloidogyne incognita em Cucumis melo L

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Espécies de Bacillus no controle de Meloidogyne incógnita e Meloidogyne javanica in vitro e na cana-de-açúcar

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Meloidogyne inornata em feijoeiro: aspectos biológicos e reação de cultivares

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Nematofauna edáfica em sistemas de uso do solo na microrregião de Chapadinha-MA

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Reproduction of Meloidogyne enterolobii in lettuce cultivars of the american group

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Biocontrole de nematoides com fungos

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Metodologias de aplicação de sulfonamida heterocíclica no controle de Meloidogyne javanica e Pratylenchus brachyurus em soja

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Inter-relações entre nematoides, fungo e a cultura da seringueira

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

The effect of gastrointestinal nematode infection level on grazing distance from dung

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Host-specificity and morphometrics of female Haemonchus contortus, H. placei and H. similis (Nematoda: Trichostrongylidae) in cattle and sheep from shared pastures in Sao Paulo State, Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evolution of the Metazoan Mitochondrial Replicase

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

New record of Pelecitus sp (Nematoda, Onchocercidae) as a parasite of Athene cunicularia (Strigiformes, Strigidae) in southeastern Brazil

The aim of this study was to report the burrowing owl Athene cunicularia as a new host for the filarid nematode Pelecitus sp. in southeastern Brazil for the first time, as well as reporting the occurrence of this nematode species in the body cavity, near the cervical air sac and lung region. This study contributes towards knowledge of parasitism in Brazilian wild birds and an anatomical region of the host as an infection site for Pelecitus sp.