Recursos humanos e materiais na assistência de enfermagem a pacientes de uma unidade de tratamento de queimaduras

The burn is among the leading causes of morbidity and mortality in our society. It shows a great complexity and is hard to treat. Beyond the physical suffering, the burned patient is affected by psychological distress, requiring a high level of knowledge for assistance planning. The Professional Practice Law No. 7498 establishes the nurse as in charge of the client, as leader of the nursing team and as responsable for the management of physical and human resources. The nurse has autonomy to design the quantitative and qualitative picture of the nursing staff and should use the methodologies for their suitability to the real levels of assistance needed. Material resources represent 15 to 25% of total expenditures at health organizations. Therefore, to maintain the care level, nurses must determine the needs, considering the quantitative, qualitative and financial aspects. The study aimed the survey of the human and material resources necessary for nursing care to patients in a Burns Treatment Unit and identify its epidemiological profile and its nursing diagnoses. We collected the data from medical records of hospitalized between July and August, and the nursing diagnoses were classified through the Taxonomy II proposed by the North American Nursing Diagnosis Association (NANDA). The design of the picture of nurses followed the parameters of COFEN Resolution nº 293/2004. We apply the Fugulin's Patients Classification System to establish levels of the required care. The institution's Cost Center provided a spreadsheet with the purchased items, subsequently classified into ABC. Most hospitalized patients were men, aged between 20 and 50. There was a predominance of patiences with minor burned and the most common type of burn was due to fire. The average residence time was 28.71 days, and 88% of the patients were discharged... (Complete abstract click electronic access below)

Análise proteômica do veneno da vespa social Polistes lanio lanio (Hymenoptera; vespidae)

Os venenos dos insetos da ordem Hymenoptera (abelhas, vespas e formigas) são responsáveis por um grande número de acidentes causados por ferroadas. Estes acidentes podem produzir uma série de reações, variando desde uma reação local, até uma reação sistêmica e anafilaxia. Estudos mostraram que 0,8% a 5% da população mundial sofrem de reações sistêmicas generalizadas após ferroadas de insetos pertencentes à ordem Hymenoptera. As espécies pertencentes ao gênero Polistes são tipicamente encontradas no sudeste do Brasil, causando muitos acidentes por ferroadas devido principalmente à proximidade dos ninhos destes insetos das habitações humanas. P.lanio lanio é uma das vespas sociais que mais causam acidentes no Estado de São Paulo e pouco se sabe sobre a composição de seu veneno. As vespas do gênero Polistes são capazes de ferroar múltiplas vezes e causar reações alérgicas severas. Dessa forma, a identificação das proteínas mais abundantes do veneno da vespa social Polistes lanio lanio por uma abordagem proteômica, se faz necessária para uma melhor compreensão dos mecanismos de ação desse veneno. A identificação de várias proteínas do veneno de P. l. lanio, revelou importantes aspectos sobre o processo de envenenamento por vespas do gênero Polistes, os quais podem ajudar no melhor entendimento dos mecanismos de ação destes venenos. A compreensão dos principais alérgenos é uma etapa importante para o desenvolvimento de novos extratos específicos para diagnósticos de alergia e imunoterapia de pacientes sensíveis ao veneno de vespas.

Doença do trato inferior (DITUI) em felinos doméstico

Lower urinary tract disease (LUTD) is a multifactorial disease, wich may be caused by uroliths and calculations, bacterial and viral infections and anatomical abnormalities, among others. The disease can also be of unknown origin in wich case it is called intersticial cystitis. Knowing that the disease may have many causes, it is necessary to know the exact etiology and the best method of diagnosis, to establish specific therapeutic measures for each case. The diagnostic methods most commonly used are: urinalysis, radiography and ultrassonography, but the blood count and biochemistry can be used, trying to assess the overall status of the animal. The terapy used depends on the causative agent of disease, wich may include changes in supply until emergency treatment, azotemia in cats with post-renal. The present study aims to present a complete study of a disease, addressing it’s etiology, diagnosis and treatment, with particular attention to first item

Anatomia foliar de espécies de Bulbophyllum, seção Micranthae Barb. Rodr. (Orchidaceae) e sua correlação com a taxonomia

Orchidaceae é uma das maiores famílias de Angiospermas, com distribuição cosmopolita, sendo que Bulbophyllum é o maior gênero da família, com mais de 1200 espécies. A seção Micranthae apresenta 12 espécies distribuídas na América do Sul e com grande representatividade no Brasil. A interpretação das Orchidaceae de maneira geral, é dificultada pela grande diversidade de espécies, gerando problemas taxonômicos. Visando levantar caracteres diagnósticos para as espécies e estados de caráter compartilhados entre elas, foram realizados estudos anatômicos das folhas de 11 espécies de Bulbophyllum seção Micranthae e de outras três espécies de Bulbophyllum constituindo o grupo externo. As estruturas anatômicas: epiderme unisseriada com cera epicuticular espessa; estômatos tetracíticos com câmaras supraestomáticas; presença de hipoderme e de feixes vasculares colaterais caracterizam as espécies estudadas. A forma cilíndrica da folha de Bulbophyllum insectiferum Barb. Rodr. é caráter diagnóstico da espécie, assim como a ausência de idioblastos traqueoidais de paredes espessadas em Dendrobium kingianum Bidwill ex Lindl. A morfologia das folhas, forma da lâmina foliar em secção transversal, forma das células epidérmicas em vista frontal, tipo de mesofilo, tipos de cristais e número de feixes vasculares são caracteres que permitem agrupamentos entre as espécies analisadas. Esses caracteres serão utilizados numa futura análise cladística procurando auxiliar a filogenia do grupo

Aplicação dos princípios de radioproteção em medicina nuclear

The Nuclear Medicine is a medical specialty which uses different radioisotopes for diagnostic and therapeutic purposes. The isotopes are radioactive elements which are administered in vivo and present distribution to specific organs or cell types. The knowledge of radioactivity and notions related to ionizing radiation allow to contextualize the radiological protection measures to be taken in Nuclear Medicine. So it is possible to minimize unnecessary exposure to patients, the public, and individuals occupationally exposed and the environmental. For this it is necessary to relate the physical and technological bases apply to this mode with the standards established by regulatory agencies, including the CNEN (National Nuclear Energy Commission) and ANVISA (National Agency for Sanitary Vigilance). In this scenario, it is important that the theoretical endorse the activities which are periodically audited for verification of compliance with the standards that aim to radioprotection. One role of the Medical Physicist in these services is, therefore, act as Radiation Protection Supervisor exerting numerous activities and ensuring compliance with these standards. In this context the stage in the area of Nuclear Medicine was developed in many customers of the enterprise Rad Dimenstein & Associados LTDA, among them the hospitals Israelita Albert Einstein (HIAE), Nossa Senhora de Lourdes (HNSL), Santa Paula (HSP), Cruz Azul (CRAZ), Grupo Fleury, among other clinics. Following the routine and then carrying out various activities pertaining to the Medical Physicist in the area, it was noted that the measures and actions are extremely effective and fundamental in terms of radiological protection

A atividade física na saúde mental: uma revisão de literatura

Ao longo da história, a doença mental passou por diversos períodos de críticas relações com a própria humanidade, exposta à vulnerabilidade dos mitos e pré-concepções que motivaram a perseguição e segregação daqueles seres humanos qualificados como “insanos”. No Brasil, as primeiras intervenções específicas de atendimento à doença mental só ocorreram a partir da segunda metade do século 19. Atualmente a doença mental representa um dos maiores gastos da rede do SUS (Sistema Único de Saúde) (Ministério da Saúde do Brasil, 1999). Nas últimas décadas, a fim de diminuir o tempo de internação e o gasto com medicamentos, um conjunto de iniciativas surgiu como forma de transformar a compreensão cultural e a relação da sociedade com as pessoas que apresentam transtornos mentais. Dentre as novas propostas humanizadoras de atendimento, a educação física e, particularmente, o exercício, vieram mostrar sua contribuição nessa área. Para Silva (1995), a atividade física acarreta uma complexa rede de reações bioenergéticas no organismo e essas acabam por melhorar o rendimento físico e mental dos pacientes nas atividades de vida diária. Tendo em vista a relevância da intervenção através da atividade física no campo da doença mental, o objetivo deste trabalho foi analisar resultados dos estudos que demonstraram os benefícios da atividade física para indivíduos com transtornos psiquiátricos, considerando os aspectos fisiológicos e emocionais dos mesmos, através de revisão da literatura científica. Para isso, foi feito um levantamento nas principais bases de dados através do cruzamento de palavras chaves, tais como “saúde mental e atividade física”, “doença mental e atividade física”, “esquizofrenia e atividade física”, “transtorno psiquiátrico e atividade física”, “transtorno bipolar e atividade física”. Os resultados dos estudos revelaram que a atividade física possui...

Espondilomielopatia cervical caudal em cães: diagnóstico por imagem

The Caudal Cervical Spondylomyelopathy, also known as “Wobbler syndrome” is a neurological disorder that affects mainly breeds of large and giant size, especially Doberman pinsher and Great danes. Its aetiology is multifactorial and leads to a narrowing of the spinal canal by morphological and positional caudal cervical vertebrae (C5, C6 and C7), causing compression of the spinal cord and nerve roots. The clinical signs presented by the affected animals are progressive ataxia of hindlimbs and, later, the forelimbs, sometimes progressing to tetraparesis. Neck pain may be present. The diagnosis is made through the association of clinical signs and diagnostic imaging such as radiography, myelography, computed tomography and magnetic resonance imaging. According to the classification of the lesion obtained by imaging examinations, the conservative or surgical treatment is established and the prognosis is variable in accordance with the degree of affection of the spinal cord

Necrose Asséptica da cabeça do fêmur

Legg-Calvé-Perthes disease is a non-inflammatory aseptic necrosis of the head of the femur that is found in both young animals and humans before the gap in the femur head closes. In the fields of both human and veterinary medicine the cause of this condition is not known for certain. Various factors have been put forward in the literature as being responsible for the incidence of this condition such as: abnormalities in coagulation, changes in blood flow in the arteries, a septic obstruction in the draining of the epiphysis or the upper parts of the femur, trauma, growth cycle, hyperactivity in a child, genetic influences and dietary factors. Case histories in dogs show that the first stages of the condition progress slowly but that limping or putting weight on the limb worsens at 6 to 8 weeks. Some owners talk about a sharp onset in clinical lameness. Other clinical symptoms may include irritability, loss of appetite and knawing at the hair surrounding the affected hip. In the course of physical examination manipulating the hip joint will cause pain to the animal. The advanced stages of the disease may result in restricted amplitude of movement, muscular atrophy and fracturing. In humans the clinical signs are similar, although progression of the disease is slower so that it can be diagnosed at an earlier stage. In veterinary medicine the diagnosis is, in the main, based on case history, clinical symptoms, physical examination and certain related procedures such as radiography. The various diagnoses include physical trauma and dislocation of the medial patella. In human medicine many people have been correctly diagnosed. Whatsmore, there is a range of related procedures that are virtually not available to veterinary medicine such as magnetic nuclear resonance, that show up necroses with great clarity before radiography and cintilography do, and is considered... (Complete abstract click electronic access below)

Freqüência do parvovírus suíno em fetos mumificados, abortados e natimortos detectado através da reação em cadeia pela polimerase (PCR)

Porcine parvovirus (PPV) is associated with reproductive failure and it has been found worldwide, including Brazil. Many diagnostic procedures are used for its detection, for example, immunofluorescence, HA, HI and PCR and this is an important technique because it is very specific and sensitive. In this work, the presence of PPV in fetuses from swine farms with reproductive problems was detected by PCR. All of 170 samples from aborted fetuses, mummies or stillborns were sampled by PCR with primers designed to VP2 region of PPV and c-myc (endogenous control). Only 142 samples (83,53%) were positive for c-myc and among them six samples (4,22%) were positive for PPV which were tested in HA. In this test, erythrocytes suspension 1% was used and three samples (50%) agglutinated but they presented low titer (4). For this work, porcine parvovirus was detected in the samples analyzed by PCR and HA. It is important to emphasize the use of endogenous control when material with elevated degree of autolysis is examined

Estudos citogenéticos de mutações na transformação de neoplasias benignas em malignas do sistema musculoesquelético

O câncer tem sido alvo de incessantes pesquisas sobre sua etiologia, desenvolvimento, progresso e tratamentos. Sua importância no campo científico se dá pela sua alta taxa de mortalidade e morbidade. O fato de que seja uma doença genética, que pode ter interferência ambiental e dietética, está cada vez mais elucidado, porém ainda existem muitos mecanismos a serem desvendados. As neoplasias ósseas, benignas ou malignas, e processos inflamatórios ósseos acometem desde crianças até adultos e idosos, podendo causar danos físicos, incapacidade motora e até a morte. Portanto, presumir o potencial de transformação maligna das lesões benignas, agressividade tumoral, capacidade de invasão tecidual, probabilidade de recidiva, propensão ao desenvolvimento de metástases e resposta ao tratamento, é um valioso expediente na escolha da proposta terapêutica. Estudos genéticos e citogenéticos têm ajudado a aumentar o entendimento sobre a carcinogênese, progressão tumoral, prognósticos e diagnósticos. Portanto, este estudo teve como objetivo detectar mutações e marcadores cromossômicos consistentes e recorrentes na transformação de tumores benignos em malignos no sistema musculoesquelético, através de análises com citogenética clássica, e relacionar estes achados com o prognóstico e diagnóstico dos pacientes. Dentre os casos coletados e analisados citogeneticamente, foram selecionados três casos de lesões ósseas benignas e três lesões ósseas malignas tidas como a progressão tumoral dos respectivos casos benignos, para discussão e relato de caso. Os achados citogenéticos relataram consistentes alterações numéricas e estruturais clonais em regiões cromossômicas com genes importantes envolvidos na progressão tumoral. Pode-se citar a perda da região contendo o gene TP53, supressor tumoral, tanto em lesões benignas como em maligna, como um dos achados mais relevantes neste estudo. Dessa forma, os resultados ...

Avaliação ambiental em empresas do distrito industrial Antônio Crepaldi, Presidente Prudente/SP

The sustainable development is the center of many discussions about the environment, economy and society. Environmental issues bring out problems with the fauna and flora, water resources, land use, population growth, residues, basic sanitation, and others. In this sense, the aim of the present work was to develop an environmental diagnostics and propose measures to mitigate the most significant environmental impacts generated by four enterprises in Antonio Crepaldi Industrial District, located at the city of Presidente Prudente/SP. In the study, it was used the methodology proposed by Moreira (2006), and it was done the following activities: monitoring and analyses of the production process, identification of potential environmental aspects and impacts; assessment of the impacts according to its nature, relevance and significance; proposition of measures to mitigate the critical impacts. As a final result, it was obtained an environmental diagnosis about the situation of each of the four companies studied, and it was possible to know the level of criticality of the impacts in each one, and proposing mitigation measures to control or minimizing them, bringing many benefits to the organization

Diagnóstico e tratamento de doença policística renal em gatos

The policystycal kidney disease that's a disease congenital autossomal dominant , no connected with the sex , what affect equally manly and females, especially the from race Persian and intersections with animals of that race. The polycystical kidney disease is broadcast the all issue from the individuals affected due at your character hereditary dominant. Yours signals clinical is linked together at the classic signals of Insufficiency Renal Account, such as: nefromegaly, poliury, anorexic, hematury and anemia , because in as much as the animals affected they tend developing this pathology among others secondary systemic problems . The means diagnostic, to ide ntify this pathology as varied, being used principally the of image, permitting a precociou diagnostic. Your treatment consists in master the symptoms caused by kidney insufficiencies Account and furnish, comfort and quality of life for the animals bearers, because it doesn’t have cur. Know the etiology, signals clinical, mediums diagnostic and the treatment is essential tools about to the screening of that disease and also to the future from race Persian and yours intersections obtained across her. The main objective this study was realize a bibliographic review about polycystical kidney disease linking a traditional forms of the diagnostic, treatment and control with new studies recently published about affected felines populations

Cardiomiopatia hipertrófica felina: revisão bibliografica

Hypertrophic cardiomyopathy (HCM) is the most common heart disease in the feline specie, more frequently affecting pure-breed males such as Ragdolls and Maine Coons. HCM can be primary (idiopathic) or secondary, when other diseases such as hyperthyroidism are involved. The disease is characterized by an increase in the diameter and thickness of the left ventricular wall, with consequent diastolic dysfunction. Mitral regurgitation happens due to compromised ventricular filling, leading to an increased left atrium size and consequent cardiogenic pulmonary edema. Along with the progress of modern veterinary medicine, many diseases could be addressed more successfully on small animal internal medicine, such as feline HCM. This article brings a literature review of the feline hypertrophic cardiomyopathy, focusing on its etiology, physiopathology, clinical presentations, diagnostic methods, therapeutics and prognosis

Desenvolvimento da técnica de MALDI Imaging utilizando tecidos animais

Currently the study of important molecular compounds present in low abundance in some tissues has been a challenge for proteomic analysis classic. An analysis requires more exploratory investigation of small regions of a tissue or a group of cells. MALDI Imaging Technology (MSI) is an application of mass spectrometry facing the chemical analysis of intact tissues. Thus, advances in mass spectrometry MALDI being obtained by the integration of histology, the best methods and automation are the main tools of data analysis. This tool has become essential to analyze the spatial distribution of peptides and proteins throughout the tissue sections, providing an enormous amount of data with minimum sample preparation. Thus, the aim of this study was to develop the technique of MALDI Imaging using tissue from glioblastoma multiforme (GBM), a form of most common malignant tumor in the brain. For this we used the printer chemical ChIP-1000 (Chemical Inkjet Printer, Shimadzu) and mass spectrometer type Maldi-ToF-ToF (Axima Performance, Shimadzu), a search of the identifications were performed in databases such as SwissProt. We identified more than forty proteins with diverse functions such as proteins F-actin-capping and Thymosin to the structure and organization cellular and proteins such several Tumor necrosis factor receptor development-related pathology. The development of this technique will permit to carry-out proteomic analysis directly into the tissue, enabling earlier diagnosis of diseases, as well as the identification and characterization of potential biomarkers of disease.

Influência da luz LED na cultura celular de Mieloma múltiplo murino–NS1 in vitro

A monoclonal antibody (mAb) is an important tool in medical biotechnology and the production of biopharmaceuticals, especially for disease diagnosis and treatment of infections, because the antibodies have a significant advantage over chemical agents used in conventional therapies . The last thirty years the technology of production of monoclonal antibodies developed mainly the technique of obtaining in vitro, but also of their production is laborious, the cost is high. A major element of the high cost of production is the fact that the long-term culture consumes a large amount of imported inputs with high added value. A major contribution of this work is to promote cell growth more quickly and efficiently. Currently, a great race to discover new technologies and techniques to synthesize new antibodies and significantly increase the production of murine mAbs. New technologies such as laser and LED are innovations and widespread in modern life, so much so that its use has proliferated worldwide, primarily in the medical field. Recent studies show a series of results from the influence of the LED light in biological tissues such as: increasing the rate of cell proliferation, increased production rate of fibroblasts, increasing the rate of synthesis of RNA and DNA synthesis of ATP, etc. To assess the contribution of the LED in the culture of Myeloma NS1murino compared to the standard procedure. - NS1 cells were provided and followed the criteria of culture medium of the Laboratory of Cellular Engineering Center of Botucatu (POPs). The same amount of cells was grown in bottles of 25 cm2 polystyrene Tissue Culture Treated, specifically marked and kept in special medium RPMI 1640 Gibco BRL  supplemented with fetal bovine serum 10%, essential amino acids and non-essential, glucose, insulin and antibiotics. It was used in LEDs Cromatek wavelength of 630nm, 475nm and 530nm. The groups were... (Complete abstract click electronic access below)