Expansão ex vivo de células uroteliais em scaffolds 3D

Malformations and possible damages to the urogenital system can be originated in the embryonic period. Moreover, fire guns, knives and accidents, where there is the disruption of the urethra, also cause these lesions. The objective was to analyze the contribution of tissue engineering in the construction of neo-urethra, developed by bioengineering. We performed an urothelial ex vivo expansion of cells in 3D scaffolds (platelet gel matrix and acellular porcine aorta) to assess the contribution of this technique in the construction of a neo-urethra. Mechanical dissociation was made of the inner wall of 10 North Folk rabbit’s bladder, weighing 2.5 to 3.0 kg. After dissociation the cell content was centrifuged and obtained a pellet of urothelial cells. The pellet was ressuspended in culture medium DMEM F12 and cells were maintained in culture for 15 days. Immunohistochemical analysis characterized the urothelial culture. The cells were then implanted in the scaffold - platelet gel. In a second experiment using aortic porcine acellular matrix were implanted urothelial cells alone and urothelial cells on platelet gel, on the inner wall of the scaffold - aorta, with space for setting bordered by a urethral probe. The complex probe - cells - aorta and probe - cells in platelet gel - aorta, were sealed with suture material and culture were maintained in a humidified 37ºC incubator with 5% CO2 in air for 12 days to subsequent histological analysis of urothelium cell adhesion to the scaffolds. By observation under an optical microscope, we could see the growth of cells in the scaffold platelet gel, from a monolayer in to a three-dimensional structure. In the acellular porcine aortic matrix containing the platelet gel, we could observe a few quantity of urothelial cells adhered. However with the acellular porcine aortic matrix in which was implanted only the urothelial cells, we have obtained adhesion to the wall

Chuva, vulnerabilidade social e percepção do risco na cidade de São Paulo: impactos de eventos extremos nos bairros da Freguesia do Ó e do M’Boi Mirim

The city of São Paulo has, since the beginning of the expansion of its urban sprawl, represented relations of conflicts and contradictions between society and nature. Once the way society relates to nature is defined by how the social agents themselves correlate in certain social and historical contexts, the ways of interaction between the social and natural elements will be different according to the forms of production and appropriation of the urban space. Even more evident is the case of the great national metropolis, given its demographic dimensions and historical conditions, the process of urban expansion follows a logic where spaces of better quality for housing are occupied by those of better financial conditions. Thus, although there are exceptions, the poorest people live in places less desirable, in less resilient environments of lower environmental quality, relating to nature and its phenomena with greater risks. These risks are reflected here as recurring flooding, mudslides and landslides for which the rain is constantly blamed. So we have a situation where it is clear that a weather phenomenon differently interacts with different social groups. In this context, the study was conducted to compare extreme events occurred in two regions of São Paulo: the Freguesia do O, in the north side, and M'Boi Mirim, in the south side. Both are regions with large number of risk areas and are in the same urban climate unit. However, they present different conditions of social vulnerability. With the investigation of each extreme rain event occurred in the two regions, in the period of 2000-2010, supplemented by field research, we tried to observe how far the rain, with his intensity and volume, can, in fact, be related to the accidents.