Cultivo celular in vitro: importância para a pesquisa biomédica e dimensão da problemática de autenticação de linhagens celulares

Experimental models composed by human and animal cell lines are simplified and informative, allowing them to be widely used for biomedical research. Most laboratories that use in vitro cultivated cells maintain a variation of cell lines stored and cultivated. Therefore, misidentification and cross-contamination events can happen during cell lines handling. This problem can generate a repertoire of dubious results and papers, which may prejudice biomedical research. Recently it was created the International Cell Line Authentication Committee (ICLAC), which aims to spread knowledge about cross-contamination and misidentification of in vitro cell lines. Despite of the efforts spent trying to aware scientific community about the importance of the correct identification of cells, the number of papers based on misidentified cell lines it´s still worrying, compromising the reliability of out coming results and conclusions regarding them. The present study aims to analyze and discuss the main advantages and limitations of eukaryote in vitro cell lines use, characterizing the cell lines authentication problems. Therefore, compilation and critical analyses of literature data was realized, aiming to improve the understanding about this subject. Based on information about 445 cell lines with issues published by ICLAC it´s clear that contamination in human cell lines represented 89,2 % of mentioned problems. HeLa cell line was the responsible for most contamination, especially in 92 normal tissue cell lines, representing 44,6% of the contamination. These results reinforce the importance of periodic maintenance of cell lines cultures by labs and implementation of authentication methods as polymorphic STRs, besides obtaining cell lines from reliable sources and cell banks

Influência da luz LED na cultura celular de Mieloma múltiplo murino–NS1 in vitro

A monoclonal antibody (mAb) is an important tool in medical biotechnology and the production of biopharmaceuticals, especially for disease diagnosis and treatment of infections, because the antibodies have a significant advantage over chemical agents used in conventional therapies . The last thirty years the technology of production of monoclonal antibodies developed mainly the technique of obtaining in vitro, but also of their production is laborious, the cost is high. A major element of the high cost of production is the fact that the long-term culture consumes a large amount of imported inputs with high added value. A major contribution of this work is to promote cell growth more quickly and efficiently. Currently, a great race to discover new technologies and techniques to synthesize new antibodies and significantly increase the production of murine mAbs. New technologies such as laser and LED are innovations and widespread in modern life, so much so that its use has proliferated worldwide, primarily in the medical field. Recent studies show a series of results from the influence of the LED light in biological tissues such as: increasing the rate of cell proliferation, increased production rate of fibroblasts, increasing the rate of synthesis of RNA and DNA synthesis of ATP, etc. To assess the contribution of the LED in the culture of Myeloma NS1murino compared to the standard procedure. - NS1 cells were provided and followed the criteria of culture medium of the Laboratory of Cellular Engineering Center of Botucatu (POPs). The same amount of cells was grown in bottles of 25 cm2 polystyrene Tissue Culture Treated, specifically marked and kept in special medium RPMI 1640 Gibco BRL  supplemented with fetal bovine serum 10%, essential amino acids and non-essential, glucose, insulin and antibiotics. It was used in LEDs Cromatek wavelength of 630nm, 475nm and 530nm. The groups were... (Complete abstract click electronic access below)

Avaliação da proliferação celular na próstata de gerbilos vasectomizados, após indução química pela injeção intraperitoneal do carcinógeno N-metil-N-nitrosuréia (MNU)

Prostate cancer is the second leading cause of cancer death in the country. Due to this factor, the interest of showing what relationship exists between vasectomy surgery and the incidence of prostate carcinoma has started. Some epidemiological studies showed an increased risk of prostate cancer in vasectomized men (Emard et al., 2001). On the other hand other authors have argued that there is no correlation (Patel et al., 2005) and there are those who said that vasectomy is linked to reduced risk of prostate cancer (Ross, 1983). Faced with an analysis of published works, such discussion remains today. The vasectomy, or deferentectomia, is a contraceptive male method, which is the section of the vas deferens of man by preventing the sperm from being expelled along with the seminal fluid during ejaculation, and is one of the most simple, economical, uncomplicated post- operative. This study aims to evaluate the process of cell proliferation and to evaluate immuno-histochemically the expression of specific markers of PCNA (Proliferating Cell Nuclear Antigen) and Ki-67, in the prostate of the gerbil after chemical induction by intraperitoneal injection of the carcinogen N-methyl -N-nitrosourea (MNU), because the regulation of the functional balance between cell proliferation and apoptosis is associated with hyperplasia and prostatic carcinoma. The experimental procedure was performed at the Laboratory of the Department of Anatomy, along with collecting the data for later analysis

Interferência eletromagnética em equipamentos de diatermia por microondas por aparelhos telefonia móvel celular GSM

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Efêmera: uma cidade em movimento: intervenção na linha férrea de Presidente Prudente

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Aikido: o movimento entre a luta e a arte

Aikido é uma arte marcial japonesa que, como todas as outras, tem alguns princípios que norteiam sua prática. Do ponto de vista da estrutura e desenvolvimento de suas técnicas, o Aikido carrega uma peculiaridade por não haver combate em seus treinamentos. É freqüentemente lembrado por essa característica. Na tentativa de descrever como se desenvolve a técnica de uma arte marcial em que não existe a competição entre os praticantes, o Aikido é treinado em katas. Katas são técnicas combinadas, em que o Uke (quem sofre a técnica) se deixa ser conduzido pelo Nague (quem aplica a técnica), para o treinamento da movimentação. Esta pesquisa assume como objeto de estudo a dúvida que persiste sobre o sentido de uma arte marcial em que não é colocada a prova sua eficiência, considerando que as artes marciais são por princípio artes de guerra. Neste recorte cartografamos a fronteira entre a cultura ocidental e a cultura oriental. Identificamos as marcas dessa arte, pois sua finalidade supera a questão do guerrear e passa diretamente para o estudo das possibilidades do corpo, este compreendido de maneira holística O corpus de nosso estudo se constitui dos recortes discursivos recolhidos nos registros das vivências de praticantes de Aikido. No campo tenso demarcado entre a cultura ocidental e a cultura oriental cartografamos o corpo em seus movimentos, no âmbito das possibilidades que o Aikido nos indica.

Proliferação e Morte Celular (Apoptose) avaliadas em ratos Lewis após tratamento com mistura de praguicidas em baixas doses

The use of pesticides in Brazil has intensified over the years. And since 2009 it was ranked as the largest market for pesticides worldwide. The consequential diffuse contamination of the environment is therefore due to the increasing number of chemicals of different classes, origins and modes of action. Little is known about the action of pesticides on human health in situations of co-exposure. Possible toxic effects are not restricted to agricultural and industrial workers, but also the general population that may be exposed continuously to its residues in food and water. Although these pesticides are mostly present in the environment at low doses, it must be considered that possible cumulative or synergistic effects may occur when there are concurrent or sustained exposure for two or more of these agents, which can lead to late manifestation of subclinical damages, sometimes irreversible. Thus, the specific objective of this study was to assess the effect of carcinogenesis promotion of a mixture of pesticides at low doses and analyze the phenomena of cell proliferation and apoptosis in rat liver. A total of 50 male Lewis rats was separated into 5 groups for 8 weeks in a medium term hepatocarcinogenesis model. The three different classes of pesticides (dieldrin, dicofol, endosulfan, dichlorvos and permethrin), whose residues were detected by ANVISA during the period from 2001 to 2005 in tomatoes cultures, were added to the feed of rats initiated to hepatocarcinogenesis with diethylnitrosamine (DEN- 200mg/kg ip). We used two different mixtures, one with no toxic effects at doses (MEX1) referring to the NOEL (no-observed-effect level) and another at doses LOEL / LEL / LOAEL (Lowest-observed-effect level / Lowest-effect level / Lowest -observed-adverse-effect level), to the installation of adverse effects (MEX2), derived from chronic studies. All animals ...(Complete abstract click electronic access below)

Cálculo fracionário aplicado ao movimento browniano

This work aims to study several diffusive regimes, especially Brownian motion. We deal with problems involving anomalous diffusion using the method of fractional derivatives and fractional integrals. We introduce concepts of fractional calculus and apply it to the generalized Langevin equation. Through the fractional Laplace transform we calculate the values of diffusion coefficients for two super diffusive cases, verifying the validity of the method

Análise da estabilidade do movimento rotacional de satélites artificiais com quatérnions e sob a influência de torques externos

The aim of this work is to analyze the stability of the rotational motion’s artificial satellite using the Routh Hurwitz Algorithm (CRH) and the quaternions to describe the satellite’s attitude. This algorithm allows the investigation of the stability of the motion using the coefficients of the characteristic equation associated with the equation of the rotational motion in the linear form. The equations of the rotational motion are given by the four cinematic equations for the quaternion and the three equations of Euler for the spin velocity’s components. In the Euler equations are included the components of the gravity gradient torque (TGG) and the solar radiation torque (TRS). The TGG is generated by the difference of the Earth gravity force direction and intensity actuating on each satellite mass element and it depends on the mass distribution and the form of the satellite. The TRS is created by changing of the linear momentum, which happens due to the interactions of solar photons with the satellite surface. The equilibrium points are gotten by the equation of rotational motion and the CRH is applied in the linear form of these equations. Simulations are developed for small and medium satellites, but the gotten equilibrium points are not stable by CRH. However, when some of the eigenvalues of the characteristic equation are analyzed, it is found some equilibrium points which can be pointed out as stables for an interval of the time, due to small magnitude of the real part of these eigenvalue

Os movimentos nas redes e em redes: das manifestações internacionais ao Movimento Prudente

Neste trabalho apresentamos uma leitura sobre o processo de articulação dos movimentos em redes e do uso das redes sociais pelos movimentos socioespaciais que mais se destacaram no mundo nos últimos cinco anos. Tendo suas bases nos marcos conceituais de movimentos socioespacial, movimentos socioterritorial, Rede e Redes Sociais, remontamos ao processo de formação da Via Campesina e das manifestações na Primavera Árabe, M12M, M15M, Occupy Wall Street, Movimento Passe Livre e o Movimento Prudente na escala local. Analisar e comparar estes movimentos e suas manifestações nos permitiu identificar a conexão entre a difusão de informações nas redes sociais e a materialização das manifestações no espaço público. Destaca-se que o uso destas tem acarretado em uma nova dinâmica não só na difusão de manifestação, mas também na realização de trabalho de base, caracterizando-se como um importante espaço de socialização política da atualidade

Biomateriais e terapia celular na reparação da cartilagem articular em equinos

Articular cartilage is the structure that coats the bone ends in regions where two bones are articulated, allowing movement. It has inefficient intrinsic and extrinsic mechanisms of repair, usually resulting in fibrocartilage formation after injury. Such repair have lower strength, stiffness and usability features when compared to hyaline cartilage. The mesenchymal stem cells have the potential to regenerate tissue without the production of scar, and because of this feature it is well studied. But to have its maximum chondrogenic potential, it is necessary to use scaffolds and growth factors. Biomaterials play the role of scaffold for the cells allowing them to become attached, grow and produce extracellular matrix, leading to formation of repair with hyaline cartilage. In this sense, the purpose of this study is to provide information on the various studies using cell therapy and / or biomaterials to produce hyaline cartilage

Células-Tronco e Terapia Celular: Suporte didático para o ensino fundamental e médio

Nos últimos anos, as pesquisas com células-tronco têm despertado um grande interesse nos meios de comunicação, levando a população a encarar esse novo conhecimento científico como sendo um método promissor para a cura de inúmeras doenças até agora, tidas como incuráveis. Deste modo, mostra-se fundamental que o ensino de Biologia se volte para o desenvolvimento de competências que permitam ao aluno entender, discutir, questionar, analisar, adquirir um posicionamento crítico diante das informações postas e fazendo uso dos conhecimentos adquiridos, servir como multiplicadores do conhecimento científico. Este trabalho tem como objetivo central a produção de um material didático contendo informações a respeito das células-tronco, quanto aos tipos, características, classificações, métodos de coleta e as terapias desenvolvidas no Brasil. Informações estas que servem como auxílio para professores de Ciências e Biologia, na compreensão e atualização perante as inovações científicas relacionadas às células-tronco. O capítulo 6 vem como uma sugestão de atividades possíveis a serem desenvolvidas a partir desse tema. Apresenta ainda um CD-ROM contendo uma síntese do assunto proposto a servir como suporte no preparo de aulas didáticas relacionadas ao tema

Mediadores da via intrínseca da morte celular programada relacionados à infecção in vitro pelo herpesvirus bovino tipo 5

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos da adição de mananoproteínas derivadas da parede celular de leveduras sobre parâmetros imunológicos de cães adultos e idosos

Pós-graduação em Medicina Veterinária - FCAV

Efeitos da dose única de parecoxibe sobre a morte celular em rins de ratos submetidos à hemorragia aguda

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)