Respostas metabólicas, morfologia do tecido muscular e expressão dos genes relacionados ao crescimento e à atrofia muscular durante o jejum e realimentação em juvenis de tilápia-do-Nilo

Pós-graduação em Zootecnia - FCAV

Avaliação ecotoxicológica de metais/metalóides e interferentes endócrinos em frações de substâncias húmicas de diferentes tamanhos moleculares

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência do cimento obturador e agente de irrigação final na infiltração marginal em situações de sobreobturações endodônticas

Objective: The objective of this paper was to verify if the final irrigation at 17% EDTA, separately or followed by chlorhexidine digluconate at 2% interferes on the apical marginal leakage in root canal overfilling, due to the use of two root canal filling materials (Sealer 26TM or SealapexTM). Methods: Forty lower incisors extracted, with a single root canal, were biomechanically prepared, at 2.0mm beyond the radicular apex, with ProTaper SystemTM, finishing it with the F3 instrument. Irrigation was accomplished with 1.0 mL NaOCl at 2.5% at each change of equipment caliper and, in the end, with 5.0 mL normal saline solution. After achieving this procedure, the foraminal channels was standardized with a file K 25 until its DO appears in the foraminal opening. From this moment, the teeth were subdivided into two groups of 20 specimens each, because of the final irrigation method used: I – irrigation with EDTA at 17% for 3min and II – identical protocol, thus, after EDTA aspiration at17%, it was again irrigated with chlorhexidine digluconate at 2%, also kept intra-canal for 3min. Each group was again sub-divided into two sub-groups (ten teeth each), according to the cement used (Sealer 26TM or SealapexTM), and filled by using a single gutta-percha F1 (ProTaperTM, Universal Filling), in such a manner that it goes 2.0 mm beyond the radicular apex. Soon after that, the teeth were immersed in Rhodamine BTM for 72h, vacuum and after the roots have been bucolingually grooved, the leakages marked were measured with program Image ToolTM. Results and Conclusions: The leakage magnitude was similar among themselves (p > 0.05), except for group Sealapex®, thus the final use with chlorhexidine reduced apical leakage (p < 0.05).

Qualidade hídrica e morfometria da microbacia do córrego da Fazenda Glória, Taquaritinga-SP

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Seleção fenotípica e identificação de snps em regiões genômicas ligados a resistência à ferrugem em Eucalyptus grandis

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Performance and parasite control of different genetic groups of lambs finished in irrigated pasture

The aim of this study was to compare the following four genetic groups of hair sheep: Santa Inês (SI), Morada Nova (MN), Brazilian Somali (BS), and the F1 1/2Dorper x 1/2Morada Nova crossbreed on traits related to growth and parasitic infection. Thirty-three male lambs of the same age and of simple birth, under the same pre-weaning management conditions were used in the experiment. After weaning the animals were housed in a completely randomized design in paddocks made of Panicum maximum cv. Tanzania. Along the course of the research, the performance of the four groups of sheep was observed to be negatively affected by gastrointestinal parasites, but there was a genotype effect to the average daily weight gain (ADWG), where the SI and F1 genotypes presented higher values. The effects of genotype, time and genotype x time interaction were significant in weight and corporal score (CS) measurements. The BS lambs had the highest CS values throughout the experiment despite not presenting greater weight gain when compared to the SI and F1 breeds. There were also significant effects of time and genotype x time interaction for packed cell volume (PCV) and FAMACHA© score (FAM) and only the time effect was significant in the total number of eggs per gram (EPG) and total plasma protein (TPP). The MN lambs showed higher PCV values and unlike the other groups, presented a FAMACHA© score below 3 and PCV above 23% even having a higher EPG tendency, especially in the initial phase, indicating a possible higher resilience to infection caused by gastrointestinal parasites.

Produtividade em espécies de Drosophila do subgrupo saltans (grupo saltans, subgênero Sophophora): efeitos da infecção por Wolbachia em linhagens normais e introgredidas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da alimentação do adulto na qualidade do endoparasitoide larval da broca comum da cana-de-açúcar

Aiming qualitative improvements in the mass rearing of Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae) in laboratory, tests were conducted with adults feed before parasitism on Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) larvae, using 14 artificial diets based on sucrose, brewer yeast and honey. The design was completely randomized with 15 treatments and five replications. The biological parameters evaluated in F1 and F2 generations were: number of adults emerged; sex ratio; adult emergence percentage; weight of pupae mass; and male and female longevity. The highest average of emerged adults were 85 in diet with sucrose 5% for F1 generation, and 91 in diet with sucrose 5% + yeast brewer 2,5% for F2; the smaller average of adult emergency were 63.60 in diet with honey 5% + yeast brewer 2.5 % for F1 generation and 66.80 in diet with honey 5% for F2. Diets that produced more females were honey 5% + yeast brewer 2.5%, showing the sex ratio of 0.82 for F1 generation, and sucrose 10% + 2.5% yeast brewer 2.5% (0.75) for F2, while sucrose 10% + yeast brewer 2.5% (0.60 for F1) and honey 2.5% (0.59) provided fewer females for F2 generation. The highest percentage of adult emergence were 93.90% with the diet containing sucrose 2.5% + yeast brewer 2.5%) and 93.89 % with honey 5% + yeast brewer 2.5%, and the lowest 81.71% (honey 5% + yeast brewer 2.5%) for F1 and 78.96 % (honey 5%) to F1 and F2, respectively. The diet with Mel 2.5% produced greater weight of pupae mass. The adult longevity did not differ significantly for all diets in F1 and F2 generations. Diets containing 5% or 10% of sucrose or honey provide, in general, improve the qualitative development of C. flavipes in mass rearing.

Cotton yield and fiber quality affected by row spacing and shading at different growth stages

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Development, characterization, and in vitro biological performance of fluconazole-loaded microemulsions for the topical treatment of cutaneous leishmaniasis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mineral requirements for growth and maintenance of F-1 Boer x Saanen male kids

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tolerance to high temperature in F-5 inbred lines of tomato

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Synaptomenal complex analysis of four breeds of Bos taurus taurus x B. taurus indicus hybrids

The synaptonemal complex (SC) was analyzed in four F1 hybrids of Bos taurus taurus and B. taurus indicus including Gyr-Simmental (G-S), Nelore Simmental (N-S), Gyr-Holstein-Friesian (G-H) and Nelore-Piemontese (N-P). We analysed the frequency of various types of SC abnormalities and the frequency of cells with SC abnormalities. The results were compared with similar observations made on purebred animals. All the animals studied possessed 29 autosomal and one sex bivalent. The frequency of cells with abnormalities in the hybrids were 28.0% in the N-P, 29.1% in the G-S, 33.3% in the N-S and 40.0% in the G-H. The frequency of cells with abnormalities in the four hybrids was 31.5%; 57.9% of these abnormalities occurred in zygotene and 42.0% occurred in pachytene. The comparisons among the hybrids and among the hybrids and their parental breeds showed that the only significant difference was between Gyr and Gyr-Holstein-Friesian animals. Some aspects of the relationship between the frequency of cells with anomalies and the fertility of hybrids are discussed.

Genistein reduces the noxious effects of in utero bisphenol A exposure on the rat prostate gland at weaning and in adulthood

Bisphenol A (BPA) is one hormonally active chemical with potential deleterious effects on reproductive organs, including breast and prostate. In contrast, genistein (GEN) is the major phytoestrogen of soy that presents potential protective effects against hormone-dependent cancers, including that of the prostate. Thus, pregnant Sprague-Dawley rats were treated with BPA at 25 or 250 μg/kg/day by gavage from gestational day (GD) 10-21 with or without dietary GEN at 250 mg/kg/chow (∼5.5 mg/kg/day). Then, male offspring from different litters were euthanized on post-natal day (PND) 21 and 180. At PND21, BPA 25 exposure induced early prostatic changes while dietary GEN attenuated some deleterious actions this xenoestrogen on epithelial cell proliferation levels, androgen receptor expression and prostatic architecture in male offspring. At PND180, a significant increase in incidence of prostatic multifocal inflammation/reactive hyperplasia and atypical hyperplasia were observed in male offspring from dams that received BPA 25. On the other hand, maternal GEN feeding attenuated some the adverse effects of BPA 25 on prostate disease at late-in-life. This way, the present findings point to preventive action of dietary GEN on deleterious effects of gestational BPA exposure in both early and late prostate development in offspring F1.