HPLC-DAD method for metabolic fingerprinting of the phenotyping of sugarcane genotypes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

High-throughput sugarcane leaf analysis using a low cost closed-vessel conductively heated digestion system and inductively coupled plasma optical emission spectroscopy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

What is the best additive to use at the ensiling of sugarcane SP81-3250?

Our objective was to determine the best additive to use for the ensiling of sugarcane SP81-3250 based on the nutritional properties and aerobic stability of the silages. The additives evaluated were (1) and (2): salt (NaCl) at rates of 1.0 or 2.0 kg/100 kg fresh sugarcane (as-is basis), respectively; (3) and (4): quicklime (CaO) at rates of 1.0 or 2.0 kg/100 kg fresh sugarcane, respectively; (5) and (6): commercial inoculants Silobac (R) and Maize All (R); and a control treatment. The addition of quicklime reduced the NDF and ADF contents of the silages by 11% compared with the control treatment, but did not increase the digestibility. On the other hand, after 24 h of in vitro fermentation, the application of 1.0 and 2.0% salt reduced the gas production by 34.15 and 33.55 mL/g OM, respectively, and the IVOMD was reduced 5.74 and 5.90%, respectively, compared with the untreated silage. Moreover, the addition of quicklime elevated the pH of the sugarcane silages, with a trend towards an increase in the DM recovery. In contrast, the bacterial inoculants did not alter the pH values, but there was an upward trend for a DM recovery. After the silos were opened, the silages that were treated with salt (independent of the dose) and 1.0% quicklime exhibited greater stability. We recommend applying 1.0 kg of quicklime to each 100 kg of sugarcane at the time of ensiling to improve the nutritional characteristics of sugarcane silage variety SP81-3250.

Brewer's yeast and sugarcane yeast as protein sources for dogs

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Building the sugarcane genome for biotechnology and identifying evolutionary trends

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Assessment of fungi in soils of sugarcane crops and their potential for production of biomass-degrading enzymes

Soil management practices are konwn to affect the biomass and enzyme activities of microbial soil communities. To assess whether burning of sugarcane prior to harvesting affects the community of soilborne fungi, we collected soil simples in two sites: burned sugarcane culture prior harvesting (BS) and non-burned sugarcane culture (NBS). A total of 75 filamentous fungal isolates were recovered from soils in both sites. Trichoderma was the most prevalent genus in both sites, followed by Fusarium, Cunninghamella and Aspergillus. The Sorensen's index (0.60) suggested a slight difference in fungi associated with both areas, with high number of fungal isolates found on BB soil. The abundance of Trichoderma isolates in NBS soil was higher than BS soil; however, the abundance of Fusarium, Aspergillus and Cunninghamella was higher in the latter type of soil. In addition, fungi isolated from BS soil showed the highest production of xylanase and laccase in comparision with fungi isolated form NBS soil. Our results indicate that the different types of sugarcane harvesting apparently did not interfere with the diversity of fungal communnities as revealed by culture-dependent methods. In addition, our data indicates the potencial of fungi from soils of sugarcane crops to produce relevant enzymes related to biomass conversion.

Improved pretreatments applied to the sugarcane bagasse and release of lignin and hemicellulose from the cellulose-enriched fractions by sulfuric acid hydrolysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Weather variables, water balance, growth, and agro industrial yield of sugarcane

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Green and brown propolis: efficient natural biocides for the control of bacterial contamination of alcoholic fermentation of distilled beverage

This study aimed to evaluate the efficiency of natural biocides, brown and green propolis, for the control of bacterial contamination in the production of sugarcane spirit. The treatments consisted of brown and green propolis extracts, ampicillin, and a control and were assessed at the beginning and end of harvest season in ten fermentation cycles. In the microbiological analyses, the lactic acid bacteria were quantified in the inoculum before and after the treatment with biocides, and the viability of yeast cells during fermentation was evaluated. The levels of acids, glycerol, total residual reducing sugars, and ethanol were analyzed for the wine resulting from each fermentation cycle. A reduction in the number of bacterial contaminants in the inoculum in the treatments with the natural biocides was observed, but it did not affect the viability of yeast cells. The control of the contaminants led to the production of higher levels of ethanol and reduced acidity in the wine produced. The results of the use of brown and green propolis to control the growth microorganisms in the fermentation of sugarcane spirit can be of great importance for using alternative strategies to synthetic antibacterials in fermentation processes including other distilled beverage or spirits.

Interaction between N-fertilizer and water availability on borer-rot complex in sugarcane

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Short-term CO2-C emissions from soil prior to sugarcane (Saccharum spp.) replanting in southern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

On the spatial and temporal dependence of CO2 emission on soil properties in sugarcane (Saccharum spp.) production

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization of typical aquatic humic substances in areas of sugarcane cultivation in Brazil using tetramethylammonium hydroxide thermochemolysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determination of uronic acids in sugarcane bagasse by anion-exchange chromatography using an electrode modified with copper nanoparticles

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)