Relação nitrogênio não proteico e proteína verdadeira em suplementos na recria de bezerros Nelore

Pós-graduação em Zootecnia - FCAV

Desempenho de novilhos Nelore confinados alimentados com óleo de linhaça in natura ou protegido

Pós-graduação em Zootecnia - FCAV

A utilização de glicerina em dietas para bovinos altera a microbiota ruminal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Degradabilidade ruminal da matéria seca de grãos de milho e de sorgo com alto ou baixo conteúdo de tanino processados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diversidade bacteriana ruminal em bovinos Nelore

Pós-graduação em Zootecnia - FCAV

Presença e tipificação de Salmonella spp. no conteúdo ruminal de bovinos pós-abate

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Combined photoelectrocatalytic/electro-Fenton process using a Pt/TiO2NTs photoanode for enhanced degradation of an azo dye: A mechanistic study

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Green tea extract and its major constituent, epigallocatechin-3-gallate, induce epithelial beta-defensin secretion and prevent beta-defensin degradation by Porphyromonas gingivalis

Background and Objective: Antimicrobial peptides, such as beta-defensins, secreted by gingival epithelial cells, are thought to play a major role in preventing periodontal diseases. In the present study, we investigated the ability of green tea polyphenols to induce human beta-defensin (hBD) secretion in gingival epithelial cells and to protect hBDs from proteolytic degradation by Porphyromonas gingivalis.Material and Methods: Gingival epithelial cells were treated with various amounts (25-200 mu g/mL) of green tea extract or epigallocatechin-3-gallate (EGCG). The secretion of hBD1 and hBD2 was measured using ELISAs, and gene expression was quantified by real-time PCR. The treatments were also carried out in the presence of specific kinase inhibitors to identify the signaling pathways involved in hBD secretion. The ability of green tea extract and EGCG to prevent hBD degradation by proteases of P. gingivalis present in a bacterial culture supernatant was evaluated by ELISA.Results: The secretion of hBD1 and hBD2 was up-regulated, in a dose-dependent manner, following the stimulation of gingival epithelial cells with a green tea extract or EGCG. Expression of the hBD gene in gingival epithelial cells treated with green tea polyphenols was also increased. EGCG-induced secretion of hBD1 and hBD2 appeared to involve extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase. Lastly, green tea extract and EGCG prevented the degradation of recombinant hBD1 and hBD2 by a culture supernatant of P. gingivalis.Conclusion: Green tea extract and EGCG, through their ability to induce hBD secretion by epithelial cells and to protect hBDs from proteolytic degradation by P. gingivalis, have the potential to strengthen the epithelial antimicrobial barrier. Future clinical studies will indicate whether these polyphenols represent a valuable therapeutic agent for treating/preventing periodontal diseases.

Effects of different mydriatics on intraocular pressure, pupil diameter, and ruminal and intestinal motility in healthy sheep

Objective To determine the effects of the administration of subconjunctival 1% atropine (SA), topical 1% atropine (A), 0.5% tropicamide (T), 1% homatropine (H), 10% phenylephrine (P), and 2% ibopamine (I) on intraocular pressure (IOP), pupil diameter (PD), ruminal motility (RM) and intestinal motility (IM) in sheep.Animal studied Ten spayed ewes of Santa Ines breed.Procedures Six experiments were performed separately at 1-week intervals. One eye was randomly selected and received one drop of A, T, H, P, I, or subconjunctival injection of atropine at 8 a. m. On the following days, IOP and PD were evaluated every 8 h until the pupil returned to its normal diameter. Ruminal motility and intestinal motility were evaluated only within the first 13 h.Results The IOP did not change significantly in the treated eyes compared with the control eyes and baseline at any time point (P > 0.05). A longer-lasting pupil dilation was observed after the administration of A (96 h), SA (79 h), H (24 h), and T (24 h). Within the first 30 min after treatment, RM and IM decreased, by 78% and 82% (H), 76% and 86% (SA), 46% and 58% (A), and 62% and 70% (T) (P < 0.001), respectively, with a tendency to return to baseline values following 13 h of drug administration. Both 10% phenylephrine and 2% ibopamine did not have any effect on the parameters evaluated (P > 0.05).Conclusions Topical and subconjunctival 1% atropine, 0.5% tropicamide, and 1% homatropine significantly reduced RM and IM, and induced pupil dilation but did not change IOP in eyes of healthy sheep. The sympathomimetics phenylephrine (10%) and ibopamine (2%) did not change the parameters evaluated.

Degradation of dipyrone by electrogenerated H2O2 combined with Fe2+ using a modified gas diffusion electrode

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of feeding corn silage inoculated with microbial additives on the ruminal fermentation, microbial protein yield, and growth performance of lambs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tratamento orgânico de resíduos de rúmen bovino

The organic wastes need to be adequately managed, in order to avoid the environmental pollution and damage to the public health. So, this work aimed to study the composting process using two methods: manual and mechanized, for the treatment of bovine ruminal waste. This residue is generated in large proportions during the bovine slaughter process, and it can lead environmental degradation and contamination, or even damage to the public health, when not treated. For the initial adjustment of the composting parameters, it was incorporated the residue of coffee husks. The manual composting system was done by the manually aerated piles, while the mechanized composting system was done in a reactor coupled to a compressor that enabled the aeration of the system. The proportions used in both systems were: 90% bovine rumen (R) and 10% coffe husks (CC); 85% bovine rumen and 15% coffe husks; 80% bovine rumen and 20% coffe husks. The parameters determined during the monitoring of the composting process were: temperature, pH, moisture, organic matter, ash, organic carbon, Kjeldahl nitrogen and C/N ratio. The results obtained during the monitoring of the piles and reactors presented similar behavior, except for the parameters Kjeldahl nitrogen and C/N ratio. When compared to the “Instrução Normativa no 25 de 23/07/2009 do Ministério da Agricultura, Pecuária e Abastecimento”, the organic produced composts with the best results were: pile 2 (85% R; 15% CC) and reactors 1 (90% R; 10% CC) e 2 (85% R; 15% CC)

Efeitos da dosagem de monensina sódica sobre o desempenho produtivo, comportamento ingestivo, saúde ruminal e características de carcaça em bovinos Nelore confinados

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Efeito das frequências de alimentação sobre o desempenho, características de carcaça e saúde ruminal de bovinos nelore confinados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)