Monitoramento de micro-organismos indicadores de higiene em linha de abate de bovinos de um matadouro-frigorífico habilitado à exportação no oeste do Paraná

Indicators microorganisms can be used as a method for evaluating the sanitary quality of food products, indicating the suitability of conditions for obtaining, processing and storage of food. This study sought to evaluate surface contamination of cattle carcasses that come from a slaughter plant under Federal Inspection in western Parana by mesophilic bacteria (AM), Enterobacteriaceae (EB), coliform at 35 degrees C (TC) and E. coli (EC). Surface samples were collected from 25 carcasses, each at 4 different points of slaughter, for non-destructive method. The samples were diluted in decimal scale and incubated in Petrifilm (R) plates. Were found averages of 1.46; 0.3; 0.23 and 0.21 log CFU / cm (2) for AM, EB, CT and EC, respectively. The values to be found were well below those recommended by the decision 471/2001 of the European Union and several other authors surveyed, indicating the effectiveness of the hygienic process industry.

Diversidade de morcegos (Mammalia, Chiroptera) em remanescentes florestais do município de Fênix, noroeste do Paraná, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A saúde bucal em pauta: análise de registros dos Conselhos Municipais de Saúde de municípios pertencentes à 17ª Regional de Saúde do Estado do Paraná, Brasil

O presente estudo teve como objetivo a análise das intervenções em saúde bucal, registradas em atas de reuniões, de 15 Conselhos Municipais de Saúde, próprios de municípios pertencentes à 17ª Regional de Saúde do Estado do Paraná. A análise documental deu-se a partir da identificação das temáticas em saúde, com ênfase na categorização por assunto das intervenções em saúde bucal. Os resultados evidenciaram os registros relativos à programação e organização da prestação de serviços, seguida pelo orçamento em saúde, como sendo os mais freqüentes do conjunto de temáticas analisadas. Pôde-se identificar, em 90 atas das 591 estudadas, o total de 134 registros de intervenções em saúde bucal. Por meio da análise desses últimos, percebeu-se que as intervenções em saúde bucal eram relatos de ações já concretizadas, desprovidas de características propositivas quando analisadas sob a dimensão do planejamento em saúde. Sinaliza-se para a necessidade da categoria odontológica de adquirir um maior padrão de representatividade nesses espaços, de forma a possibilitar vínculos importantes no processo de planejamento e de fortalecimento da saúde bucal enquanto direito de cidadania.

Genetic diversity in Egeria densa and E. najas in Jupia Reservoir, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Survey for Foot-and-mouth Disease in the Endangered Marsh Deer (Blastocerus dichotomus) from Marshlands of the Parana River Basin, Brazil

Habitat fragmentation and diseases have resulted in a decline of the marsh deer (Blastocerus (dichotomus) throughout its South American range. Our objectives were to determine whether marsh deer intended for translocation from a region of the Rio Parana Basin had been infected previously by foot-and-mouth disease virus (FMDV) and whether they were carrying virus We captured marsh deer from June to October 1998 and collected blood from 108 animals and esophageal-pharyngeal fluid from 53 Serum was tested for antibodies against three FMDV serotypes (O, A, and C) by liquid-phase-blocking sandwich enzyme-linked immunosorbent assay (ELISA) Esophageal-pharyngeal fluid was tested for FMDV RNA by reverse transcription polymerase chain reaction (RT-PCR) and inoculation into three successive baby hamster kidney (BHK-21) cell subcultures, followed by RT-PCR of cultures We detected low log(10) titers (range 1 0-1 5) to FM DV subtype A(24) Cruzeiro in 19 of 108 sampled marsh deer, but failed to isolate FMDV or detect FMDV RNA in any samples we conclude that marsh deer from our study site were unlikely to carry FMDV, however, as a preventive measure, the 19 animals with titers for FMDV were not sent to FMDV-free Brazilian states

Biochemical evidence of a possible new species of Neoplecostomus (Teleostei : Loricariidae) from the upper Rio Parana basin, Brazil

Neoplecostomus paranensis Langeam, 1990, from the upper Rio Parana, is the only Neoplecostomus species described in this basin and is distinguished from its congeners by the lack or reduction of the adipose fin. Neoplecostomus specimens with a normal and always present adipose fin were caught in the Rio Corumba, upper Rio Parana basin. In the present study two samples of populations, one from a tributary of Rio Paranapanema (identified as a typical N. paranensis) and the other from the Rio Corumba were compared through allozyme electrophoresis. Six diagnostic loci were found, Acp-A, Adh-A, Est-A, Gpi-A, Ldh-A and Ldh-B. In addition, the locus Gpi-B showed significant differences between allelic frequencies for the two samples. Nei's genetic identity between the populations was 0.731. The expressive genetic divergence together with the presence of an adipose fin show that the sample from the Rio Corumba is distinct from N. paranensis and probably represents a new species. (C) 2003 Published by Elsevier Ltd.

A new Moenkhausia Eigenmann, 1903 (Ostariophysi: Characiformes) from Chapada Diamantina, rio Paraguaçu Basin, Bahia, Northeastern Brazil

Moenkhausia diamantina, nova espécie, é descrita de tributário do rio Paraguaçu, BA, nordeste do Brasil. Esta espécie é distinguida das demais congêneres por caracteres relacionados ao padrão de coloração do corpo, pela presença de escamas na linha mediana pré-dorsal e número de raios da nadadeira anal.

Deep barcode divergence in Brazilian freshwater fishes: the case of the Sao Francisco River basin

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

DNA barcoding reveals hidden diversity in the Neotropical freshwater fish Piabina argentea (Characiformes: Characidae) from the Upper Parana Basin of Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Description of a new species of Pareiorhina (Siluriformes: Neoplecostominae) from Rio Sao Francisco basin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genetic differentiation among ten populations of the genus Neoplecostomus (Teleostei: Loricariidae) from the upper Parana River basin

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A new species of Hyphessobrycon (Characiformes : Characidae) from rio Portuguesa, rio Orinoco basin, Venezuela

A new species of Hyphessobrycon is described from tributaries of the rio Portuguesa, rio Orinoco Basin, Venezuela. The new species is diagnosed from congeners by the presence of two large hooks on each side of the anal fin in adult males ( processes of last unbranched and first branched anal-fin rays), distal third of caudal-fin black except for its distal tips ( which are hyaline), anal-fin rays v, 19-21, and dorsal-fin rays ii, 8. Comments on its generic status and putative relationships are provided.

Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Length-weight relationships for 20 species collected in the Jurumirim reservoir (Paranapanema Basin, Brazil)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A new species of Ctenobrycon Eigenmann, 1908 (Characiformes: Characidae) from the rio Orinoco basin, Venezuela

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)