Estudo da ação do inseticida Tiametoxam na sobrevivência e no comportamento de operárias de Apis mellifera L., 1758 (Hymenoptera: Apidae) em diferentes idades

The bee Apis mellifera has a great importance because it is the most economically valuable pollinator for crops worldwide, ensuring cross-pollination and increasing fruit yield. Moreover, agriculture increasingly develops chemicals to control weeds, fungi and insect pests to ensure productivity. Insecticides are used on a large scale in the state of São Paulo, in cultures of citrus for control of greening. Applications are usually made by aircraft and as a result of the effect derives a significant mortality is observed in apiaries near the plantations. Honey bees can get in contact with such chemical agent through their activities of water harvesting, plant resins, pollen and nectar. Intoxication resulting from this exposure can be lethal, which is easily detectable, or cause effects on the physiology and behavior of the insect. These, in turn are hardly detectable, such as paralysis, disorientation, behavioral changes, but can compromise the entire social structure of the colony, therefore aimed to study the effects of the insecticide Thiamethoxam behavior of honeybees A. mellifera. Newly emerged individuals and with 10 days of age were tested. Applications of 1 μL de Thiamethoxam, diluted in acetone, were made on the dorsal thorax with a microapplicator. Preliminarily, it was observed LD50 twenty four hours after topical treatment of Thiamethoxam. It was found that the LD50 for newly emerged honeybees is 8 ng/bee and for honeybees with 10 days of age is 18 ng/bee. The behaviors were analyzed 1 hour after application of insecticide at doses corresponding to LD50/100, LD50/50, LD50/10 and LD50, besides the control group. In the test reflex proboscis extension, there was impairment of behavior at doses of 8 and 18 ng/bee workers in newly emerged workers and 10 days of age, respectively. And in locomotor behavior was no change only at a dose of 18 ng/bee workers at 10 days of age... (Complete abstract click electronic access below)

Avaliação dos efeitos citotóxicos, genotóxicos e mutagênicos do fipronil, após fotodegradação, utilizando Allium cepa como organismo teste

Compounds released into the environment can induce genetic alterations in living organisms. A group of chemicals that shows proven toxicity is the pesticides, and the insecticides are the most harmful. The insecticides of the family phenylpyrazole have wide application both in agriculture and in homes. Fipronil, an insecticide of this chemical group, is widely used in various cultures and in homes, mainly for fighting fleas and ticks on dogs and cats. The use of fipronil may represent a risk to man and the environmental health, since this pesticide can potentially induce cell death, regardless of cell type. Fipronil, when in contact with the environment, can undergo various degradation processes, including photodegradation. The toxic effect of one of its metabolites derived from photodegradation, sulfone-fipronil, is approximately 20 fold as great as fipronil itself. The A. cepa test system was used to evaluate cytotoxic, genotoxicity and mutagenic effects of fipronil before and after phptodegradation. Seeds of Allium cepa were subjected to solutions of fipronil, pre-exposed or not exposed to degradation by sunlight. The germination tests were conducted both under the effect of light and in the dark. We evaluated the cumulative potential of this insecticide using 48 and 72-hours recovery tests. The results showed that when fipronil was previously exposed to the sun, it presented a greater genotoxic and mutagenic potential, showing that the metabolites formed by photodegradation can show more harmfull effects

Caracterização de resistência de genótipos de soja a Bemisia tabaci biótipo B (hemiptera: aleyrodidae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Anatomia e morfologia do sistema nervoso do diplópodo Gymnostreptus olivaceus (Spirostreptida, Spirostreptidae) e avaliação dos efeitos do inseticida deltametrina neste sistema

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interação de proteínas Vip3A e Cry1la10 de Bacillus thuringiensis com atividade inseticida a lepidópteros-praga

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Influência do volume reduzido e do estádio fenológico da planta de soja no controle da Anticarsia gemmatalis (Hübner, 1818)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Avaliação dos efeitos de concentrações subletais de dimetoato no cérebro e ventrículo de Apis mellifera africanizada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Modelos Matemáticos e Computacionais no Manejo Integrado de Pragas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo de possíveis correlações entre miastenia grave e exposiçao crônica a pesticidas em nosso meio

Pós-graduação em Bases Gerais da Cirurgia - FMB

Controle de populações de Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae) utilizando inseticida químico ou biológico

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

FIPRONIL promotes motor and behavioral changes in honey bees (APIS Mellifera) and affects the development of colonies exposed to sublethal doses

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Toxicity of imidacloprid to the stingless bee Scaptotrigona postica Latreille, 1807 (Hymenoptera: Apidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Rhamnolipids: solution against Aedes aegypti?

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Incidência de mosca-minadora e insetos vetores em sistemas de manejo de pragas em tomateiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Resistance of collard greens to Ascia monuste orseis (Lepidoptera: Pieridae)

Ascia monuste orseis (Godart) (Lepidoptera: Pieridae) is a production limiting pest in collard greens, Brassica oleracea (L.) var. acephala, cultivation. Because of the overuse and harmful effects of synthetic insecticides on nontarget species, the use of insect-resistant cultivars can be a valuable strategy in pest control. In this study, newly hatched A. monuste orseis larvae were confined to the leaves of 29 collard greens cultivars under a controlled environment to investigate plant resistance. We evaluated the incubation period, duration of instars, total duration of the immature and pupal phases, the egg to adult life cycle duration, mortality per instar, total weight of fifth instar larvae and pupae (age = 24 h) and larval and pupal survival and eclosion. Antibiosis and/or antixenosis were observed in selected cultivars. The results show that Gigante I-915 (E) exhibited high larval mortality and that the Pires 1 de Campinas cultivar (P) prevented all pupae from proceeding to the adult stage. The Introdu double dagger es do municipio de Arthur Nogueira Z (Y), Cabocla (AA), Japonesa (R) and Manteiga de Mococa (M) cultivars prolonged the larval stage. Japonesa (R) and Introdu double dagger es do municipio de Arthur Nogueira Z (Y) increased the egg to adult developmental period, and the Japonesa (R) cultivar also prolonged the pupal stage. The Verde-escura (O), Crespa de Capo Bonito (I), Couve de folhas Manteiga 900 Legitima P, Alto (AB), Gigante I-915 (E) and Manteiga Ribeiro Pires I-2446 (H) cultivars reduced the larval weight of A. monuste orseis.