The GHEP-EMPOP collaboration on mtDNA population data-A new resource for forensic casework

Mitochondrial DNA (mtDNA) population data for forensic purposes are still scarce for some populations, which may limit the evaluation of forensic evidence especially when the rarity of a haplotype needs to be determined in a database search. In order to improve the collection of mtDNA lineages from the Iberian and South American subcontinents, we here report the results of a collaborative study involving nine laboratories from the Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) and EMPOP. The individual laboratories contributed population data that were generated throughout the past 10 years, but in the majority of cases have not been made available to the scientific community. A total of 1019 haplotypes from Iberia (Basque Country, 2 general Spanish populations, 2 North and 1 Central Portugal populations), and Latin America (3 populations from São Paulo) were collected, reviewed and harmonized according to defined EMPOP criteria. The majority of data ambiguities that were found during the reviewing process (41 in total) were transcription errors confirming that the documentation process is still the most error-prone stage in reporting mtDNA population data, especially when performed manually. This GHEP-EMPOP collaboration has significantly improved the quality of the individual mtDNA datasets and adds mtDNA population data as valuable resource to the EMPOP database (www.empop.org). (C) 2010 Elsevier B.V. All rights reserved.

The copia retrotransposon and horizontal transfer in Drosophila willistoni

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cytogenetic and molecular characterization of Speothos venaticus specimens.

The bush dog (Speothos venaticus) is a South American canid, included in the IBAMA (Brazilian Institute of Environment and Renewable Natural Resources) official list of animals threatened with extinction, in the vulnerable category. As a preservation and conservation strategy, specimens kept in captivity by Brazilian Institutions are monitored by a management plan. In order to characterize and analyze the genetic variability of bush dog specimens, a cytogenetic analysts was carried out, and microsatellite data were also obtained through the use of 15 primers, originally developed for the domestic dog (Canis familiaris). All tested primers showed transferability and amplified fragment sizes similar to those described for the canine genome. From the total number of primers, eight were tested, and presented two polymorphic regions. Regarding cytogenetic analysis, one of the animals had chromosomal mosaicism,-disqualifying it as a reproducer to form stocks. Thus, we concluded that the genetic evaluation of wild animals kept in captivity provides data that can help with the practice of exchange between different institutions, avoiding problems in the reproductive capacity of the breeding stock.

Morphology and incidence of torus palatinus and mandibularis in Brazilian Indians

The incidence and morphology of torus platinus and mandibularis was verified in 200 Indians, residents of two Brazilian Indian Reserves in Sao Paulo State, Brazil. A low incidence of both types of exostoses was observed, with torus palatinus occurring more frequently than mandibularis. These structures did not occur in individuals less than 10 years of age. Flattened torus palatinus predominated in relation to the other forms.

The geotectonic environments of Early Precambrian granulites in Brazil

A review is presented concerning Archaean granulites occurring in some old domains of the South American Platform, which was consolidated at the end of the Brazilian Cycle (900-500 Ma). The rocks occur in different geotectonic environments and show variable ages, structures and lithological associations. The most important complexes are the Atlantic Granulite Belt in the São Francisco Craton and the Goias Granulite Belt in the Central Goias Massif, both several hundred kilometres long. The former is composed of the Caraibas Complex, the Jequié Complex, the Salvador Complex and several minor granulite occurrences along the Brazilian coast in the States of Espírito Santo and Rio de Janeiro. The latter includes the large basic-ultrabasic complexes of Barro Alto, Tocantins and Canabrava. Both belts consist of massive or foliated rocks, banded or homogeneous and varying from acidic to ultrabasic in composition. They are the result of metamorphism affecting diversified supra- and infracrustal material. The Atlantic Granulite Belt lies between greenstone/granite terrains which show ovoid and boomerang-type dome structures. The contacts between both are either tectonic or transitional. Another occurrence of Archaean granulites comprises intercalations of palaeosomes and melanosomes within migmatites and anatectic rocks. These vary in size from small lenses to irregular complexes which may attain sizes of several hundred square kilometres. Apart from migmatites, they are associated with gneisses, schists and granitoid bodies. They are located in regions which underwent remobilization of varying intensity during the Middle and Late Precambrian. The rocks show polymetamorphism, K-feldspar blastesis, tectonic overprinting and isotopic rejuvenation. These granulites are in some cases very similar to those formed during the Middle Precambrian. In some places it is therefore quite difficult to distinguish between Early and Middle Precambrian granulites - the more so, since interpretations of radiometric age values are largely controversial. At present there is no evidence of granulitic rocks related to the Late Precambrian geotectonic cycles of Brazil. © 1979.

Indirect fluorescent test for detection of anti-Paracoccidioides brasiliensis antibodies using coated bentonite particles as antigen

An indirect fluorescent test was developed for detecting antibodies to Paracoccidioides brasiliensis using bentonite particles as antigen (Bent-IF). The bentonite particles were coated with P. brasiliensis polysaccharide antigen and tested with sera from paracoccidioidomycosis patients (36 sera), normal blood donors (32 sera) and patients with non-mycotic diseases (29 sera). The titres given by the positive sera were compared with those of complement fixation (CF), immunodiffusion (ID) and immunofluorescent test using yeast forms of the fungus as antigen (conventional-IF). All normal blood donors' sera gave a negative Bent-IF, conventional-IF, ID and CF tests. All paracoccidioidomycosis sera were reactive in conventional-IF and gave concordant results in Bent-IF. There was no correlation between CF and Bent-IF titres. 27·6% of sera from patients with non-mycotic diseases gave weak titres in both IF-tests. The present data indicate that the Bent-IF is a sensitive and simple serodiagnostic technique comparable with the conventional P. brasiliensis antibody test. © 1983.

The effect of Ketoconazole on experimental paracoccidioidomycosis in the Syrian hamster: immunological and histopathological study

The effect of Ketoconazole (KTZ) on the hamster experimental intratesticular paracoccidioidomycosis was studied employing different treatment schedules. KTZ long course treatment beginning at an early stage of the infection was effective in preventing fungal proliferation, dissemination to lymph nodes, spleen and kidneys, and in maintaining low levels of humoral and cellular specific immune responses. KTZ short course treatment starting at an advanced stage of disease resulted in a more severe histopathological picture without significant changes in the immunological profile. The drug prolonged the life span of hamsters infected with Paracoccidioides brasiliensis, but did not prevent mortality. Toxic necrosis of the bone marrow occurred in normal animals receiving 120 mg/kg/day of KTZ but with lower doses no morphologic alterations were observed in heart, lungs, kidneys, adrenals, spleen, liver, intestine or bone marrow. © 1984 Dr W. Junk Publishers.