Avaliação da Saccharomyces cerevisiae como agente ligante na técnica de difusão em filmes finos por gradiente de concentração (DGT) para a determinação de metilmercúrio

Sabendo-se do potencial de toxicidade do mercúrio, mais especificamente de sua forma orgânica, o metilmercúrio (MeHg), faz-se necessária a busca por métodos analíticos eficientes e precisos para a determinação e especiação do metal. A técnica de difusão por gradiente em filmes finos (DGT) já vem sendo utilizada para tal fim de maneira eficiente tendo apresentado resultados satisfatórios em diversos estudos. Porém, a utilização de ligantes alternativos aos convencionais, que tornem a aplicação da técnica menos custosa em termos laborais e financeiros, faz-se importante para um monitoramento constante e eficiente do metilmercúrio no ambiente. No presente trabalho buscou-se avaliar o potencial da levedura Saccharomyces cerevisiae imobilizada em agarose em combinação com a poliacrilamida como agente difusivo para determinação seletiva de MeHg. Os resultados demonstraram-se positivo, com um coeficiente de difusão médio de 7,03 ± 0,77 x 10-6 cm2 s-1. Os resultados demonstraram também baixa influencia do pH, força iônica e outros íons na retenção do analito pelos dispositivos. As recuperações de Hg2+ demonstraram-se insatisfatórios, evidenciando a seletividade dos dispositivos

Dinâmica secular rotacional de corpos tri-axiais: expansão de V em ressonâncias de primeira ordem

Consider a finite body of mass m (C1) with moments of inertia A, B and C. This body orbits another one of mass much larger M (C2), which at first will be taken as a point, even if it is not completely spherical. The body C1, when orbit C2, performs a translational motion near a Keplerian. It will not be a Keplerian due to external disturbances. We will use two axes systems: fixed in the center of mass of C1 and other inertial. The C1 attitude, that is, the dynamic rotation of this body is know if we know how to situate mobile system according to inertial axes system. The strong influence exerted by C2 on C1, which is a flattened body, generates torques on C1, what affects its dynamics of rotation. We will obtain the mathematical formulation of this problem assuming C1 as a planet and C2 as the sun. Also applies to case of satellite and planet. In the case of Mercury-Sun system, the disturbing potential that governs rotation dynamics, for theoretical studies, necessarily have to be developed by powers of the eccentricity. As is known, such expansions are delicate because of the convergence issue. Thus, we intend to make a development until the third order (superior orders are not always achievable because of the volume of terms generated in cases of first-order resonances). By defining a modern set of canonical variables (Andoyer), we will assemble a disturbed Hamiltonian problem. The Andoyer's Variables allow to define averages, which enable us to discard short-term effects. Our results for the resonant angle variation of Mercury are in full agreement with those obtained by D'Hoedt & Lemaître (2004) and Rambaux & Bois (2004)

Estudos de dispersão de lagartas em milho e soja visando ao manejo de pragas no Brasil e EUA

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Desenvolvimento de sistema de análise para valerato de estradiol baseado em polímeros biomiméticos

Abstracts : The development of analytic methods more selective and sensitive is of great importance for a better quality in the determination of chemical species, therefore increasing the reliability of the results. In this way, the optimization of separation/concentration is still necessary. The use of Molecularly Imprinted Polymers - MIPs have demonstrated to be an efficient tool of analysis with a great potential in minimizing limitations of separation/concentration techniques traditionally employed. In general, the MIPs are obtained by polymerization in the presence of a template to be imprinted so that a polymeric skeleton is formed around the future analyte. In the present work, the template used is Estradiol Valerate (EV), compound used in the hormone replacement therapy (HRT) during climacteric. After the polymerization in bulk and in an anaerobic environment using MAA, EGDMA, AIBN, acetonitrile and VE, the obtained MIP was powdered, sifted (<120 μm) and placed in a soxhlet system containing ethanol at 60 °C, in order to remove the imprinted molecule through six successive washes in periods of 24 hours. The water used in the washings was analyzed using HPLC and spectrophotometry UV/Vis. Then, the obtained MIP was dried at room temperature and 150 mg was inset in SPE cartridges in order to evaluate the polymer's efficiency in the analyte pre-concentration and extraction. To do so, 100,0 mL of VE standard solution (2mg L-1) were pre-concentrated at 4,0 mL min-1 and eluted with 10,0 mL ethanol at 1,0 mL min-1, obtaining recoveries of 53%. Additionally, a NIP (non-imprinting polymer) was prepared to compare the obtained results, in which the recovery was 80%. In the same way, studies were conducted using commercial Strata™-X cartridges, obtaining 53% recovery. Since, the results did not reflect that than was expected, in relation with the MIP efficiency in the recovery, a computational ...

Ocorrência de reabsorção radicular no tratamento ortodôntico: revisão crítica da literatura

The root resorption is one of the possible sequels of greater complexity to the orthodontist, the difficulty of detection and be asymptomatic. The presence, during the orthodontic treatment, is linked to a multifactorial origin, a complex combination of biological activities, anatomical, physiological, genetic variables and mechanical forces. Thus, the aim of this study was perform to critical literature review related to root resorption in orthodontic movement. Were included articles published between 2004 to 2014, indexed in the PUBMED database. Used descriptors contained in the Mesh, being adopted as inclusion criteria: clinical trials in humans, only articles that had clear ideas and objective, articles with English language. The search strategy used resulted in 24 articles and after applied the inclusion and exclusion criteria, 11 articles remained. Based on the results of this study it was concluded that heavy forces of intrusion, inclination and torque leads to a greater degree of root resorption; its etiology is multifactorial, the periodic radiographic control during treatment is needed and further studies are suggested to identify more specifically the causes and risk factors for root resorption.

Efeito da umidade no esmalte dental na interação de dois sistemas de colagem ortodôntica

Objetivo: a proposta desse estudo foi avaliar, por meio de microscopia eletrônica de varredura (MEV), a interface adesiva após a descolagem de acessórios ortodônticos, fixados em dentes bovinos com emprego de adesivo hidrofílico e hidrofóbico, em condições distintas de umidade do substrato dentário. Métodos: foram utilizados 20 incisivos inferiores, divididos em quatro grupos (n = 5): Grupo I , empregou-se o sistema de colagem contendo o primer hidrofílico Transbond MIP e a pasta adesiva Transbond XT, aplicados em substrato úmido; Grupo II, empregou-se o sistema de colagem primer hidrofóbico Transbond XT e pasta adesiva em substrato úmido; Grupos III e IV, os braquetes foram colados com os mesmos sistemas de união dos Grupos I e II, porém com esmalte dentário seco. Removidos os acessórios, avaliou-se em MEV, em diferentes magnificações, a interface adesiva e a interação dos sistemas adesivos com o esmalte dentário. As imagens foram avaliadas qualitativamente, observando-se o embricamento mecânico para o adesivo hidrófilo em quaisquer condições de substrato. Resultados: a falta de umidade no esmalte condicionado permitiu uma melhor interação entre os materiais de ligação e a estrutura adamantina. O adesivo hidrofóbico apresentou o pior resultado de interação micromecânica quando aplicado a uma estrutura dentária úmida; já o sistema hidrofílico mostrou-se versátil, obtendo resultados aceitáveis em condições de umidade e excelente interação na ausência de contaminação. Conclusão: os autores afirmam que a melhor condição do esmalte dentário para a aplicação de primers é com a ausência de umidade.

Biocompatibilidade de primers e adesivo utilizados na confecção de próteses maxilofaciais implantorretidas: análise in vitro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seletividade de inseticidas ao parasitoide de ovos Trichogramma pretiosum (hymenoptera: trichogrammatidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação funcional pré e pós programa de exercício físico de pacientes em hemodialise

Model Study: An experimental study Introduction: Chronic Kidney Disease (CKD) refers to a syndromic diagnosis which leads to a progressive and irreversible loss of renal function. A hemodialysis patient may have limitations in functional capacity, pulmonary function and respiratory musclular strength impacting in quality of life. Objective: To evaluate the effects of an exercise program on pulmonary function, functional capacity, quality of life and pain in patients undergoing hemodialysis. Methodology: The study included 28 patients of both genders, women and men aged between 40 and 60 years undergoing dialysis at the Kidney Institute, Santa Casa de Misericordia in Presidente Prudente-SP. Primary outcomes included respiratory muscular strength measurements assessed by manovacuometry. The functional capacity was evaluated by a six minute walking test. A life quality questionnaire was applied to evaluate quality of life (SF36-KDQOL). Lung function was evaluated by spirometry. Pain was assessed by a visual analogue scale. The exercise program consisted of training 3 times a week for 40 minutes on hemodialysis during eight weeks. At the end of the program all patients were reassessed. Results: There was no significant difference in the values of FVC and FEV1 before and after the exercise program as well as the index Tiffenau. The value of post MIP was significantly higher than the value obtained in the pre program. For variable MEP no significant difference was found. Functional capacity evaluations showed that there were no significant differences (p> 0.05). The evaluation of quality of life, about the domains of specific areas of CKD showed statistical significance when comparing the list of symptoms and problems with overloading of renal disease and professional role. Indicators related to pain were significantly reduced after the program (P <0.05). Discussion: A chronic kidney patient faces complex situations of physical, social and financial aspects. Although no statistically significant results were found in all variables, the study corroborates to others found in the literature, which suggests that an exercise program can be positive for this population. Conclusion: Although lung capacity and functional capacity did not submit changes to the end of the study, reduced levels of pain, fatigue and dyspnea suggest improvement in functional performance after exercise programs.

Hybrid methods for lot sizing on parallel machines

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência de pressões de trabalho, espaçamentos e altura de instalação na distribuição de água de aspersor autocompensante

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Desenvolvimento de sensor baseado em polímeros molecularmente impressos para determinação de álcoois superiores em óleo fúsel

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção de impressões digitais fraudulentas utilizando padrões mapeados localmente em multiescala

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção de impressões digitais falsas no reconhecimento biométrico de pessoas

Pós-graduação em Ciência da Computação - IBILCE

Avaliação da neutralização dos efeitos citotóxicos do ácido lipoteicóico em canais radiculares

Pós-graduação em Odontologia Restauradora - ICT