Ciclo testicular e espermatogênese de Devario aequipinnatus (cypriniformes, cyprinidae) submetido a diferentes temperaturas

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Cultivo de Eucalyptus urograndis em atmosfera enriquecida com CO2: mudanças no proteoma cloroplastidial

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da distribuição das proteínas relacionadas às teneurinas no sistema nervoso central de primatas não-humanos (Sapajus spp) e ratos (Rattus norvegicus)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise da microfenda axial em cavidades classe V restauradas com resina composta e polimerizadas com diferentes fontes de luz. Estudo pela microscopia eletrônica de varredura

Pós-graduação em Ciências Odontológicas - FOAR

Influência do sistema de cimentação adesiva e da fonte de luz fotoativadora na resistência à extrusão de pinos de fibra de vidro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Híbridos orgânicos-inorgânicos siloxanos àcidos-graxos emissores de luz branca

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reparação tecidual pulpar sob ação bioestimuladora do laser de baixa intensidade (LILT), e do diodo emissor de luz (LED): estudo em macacos prego

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da luz e temperatura em cavidades e canais secretores em plântulas de Copaifera langsdorffii Desf. (Leguminosae-Caesalpinioideae): estudo anatômico e ultraestrutural

Pós-graduação em Ciências Biológicas (Botânica) - IBB

Influência da luz LED em culturas de células-tronco mesenquimais in-vitro

Stem cells are defined as cells capable of self-renewal and differentiation into specialized cells when submited to external signalings in the enviroment. Among adult stem cells, mesenchymal cells occupy an important position because they can differentiate into mesodermal cells such as osteoblasts, adipocytes and chondrocytes. Cell therapy consists in the use of mesenchymal stem cells (MSC) in the treatment of degenerative diseases and harmed tissue reconstruction. Due to the longstanding and costly procedure for cultivation of MSC, it was proposed the use of low power light sources, such as light emitting diodes (LED), to optimize these factors. Recent works have shown a series of results from the influence of LED light on biological tissues such as increased rate of cell proliferation, increased RNA, DNA and ATP synthesis rate. The purpose of this study is to compare the biomodulator effect of LED light set at wavelengths 630nm ± 10nm and 805nm ± 10nm on the mesenchymal stem cells proliferation. For this, the mesenchymal stem cells culture adopted the procedure used in the Departament of Animal Reproduction and Veterinary Radiology of the Faculty of Veterinary Medicine and Animal Sciences of Botucatu. MSC were obtained from an adult horse bone marrow, and isolated by density gradient separation, with the FICOLL reagent and by centrifugation. The pellet containing the stem cells was removed and these were placed in low glucose DMEM culture medium, containing 10% fetal calf serum and antibiotics. The material was observed daily by inverted microscopy for monitoring the progression of the cells and subsequently the amount of cells were counted in a Neubauer counting chamber. The amount of MSC was obtained by cell culture seeded in 24 wells culture plate and segregated into three distinct groups: Group 1 was irradiated with wavelength set at 630nm ± 10 nm, Group... (Complete abstract click electronic access below)

Influencia de diferentes intensidades de luz en la resistencia de unión de un sistema adhesivo utilizando un colorante fluorescente para su observación

The purpose of this study was to use a fluorescent dye and CLSM microscope to observe the effect of different light intensities on dentin tensile bond strength. Flat dentin surfaces were created on 16 intact human third molars and divided in 4 groups: Group G1 - halogen - KM -200R®; Group G2 - LED - Ultraled®; Group G3 - LED - UltraLume LED5® and Group G4 - LED - Biolux Single V®. For all the groups, the restoration procedure used Single Bond® adhesive, mixed with rodamin B and InTen-S® composite resin. Then, they were cut on serial sections to obtain 1 mm2 area and submitted to micro tensile test and after words, the fractures were analyzed with a digital microscope and CLSM. The statistical analysis showed that all in all groups, except Group G2, which had a significant smaller tensile bond strength ratio. The fracture mode analysis showed that there were significant differences when comparing groups G1 / G2, and G2 / G4. There is no evidence of relevant differences among the other groups. With these results, we conclude that the use of fluorescent dye and CLSM demonstrated to be a simple and nondestructive technique, and that there are evidences that light intensities influenced the dentine tensile.