Amperometric glucose biosensor based on layer-by-layer films of microperoxidase-11 and liposome-encapsulated glucose oxidase

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspergillus niger beta-Glucosidase Has a Cellulase-like Tadpole Molecular Shape INSIGHTS INTO GLYCOSIDE HYDROLASE FAMILY 3 (GH3) beta-GLUCOSIDASE STRUCTURE AND FUNCTION

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate

Zymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 10(7) CFU mL(-1) by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30 degrees C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 10(9) CFU mL(-1) during fermentation time.

Doses de nitrogênio em cobertura no feijoeiro em sucessão a gramíneas no cultivo de inverno-primavera

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

USO DA CERCLAGEM E RESINA ACRÍLICA EM FRATURAS MANDIBULARES DOS EQUÍDEOS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Investigação da condução elétrica em fitas duplas de dna imobilizadas em eletrodos de ouro por medidas eletroquímicas

Pós-graduação em Química - IQ

Desenvolvimento de biossensor eletroquímico para diagnóstico de dengue

Pós-graduação em Biotecnologia - IQ

Uso de invertase imobilizada em pó de sabugo de milho para produção de açúcar invertido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e imobilização de celulases em matriz de agarose com diferentes ativações químicas

Pós-graduação em Biotecnologia - IQ

Desenvolvimento de imunossensor impedimétrico para detecção do corante disperso Red 1

Pós-graduação em Química - IQ

Sprayed films of europium complexes toward light conversion devices

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Iliac osteosynthesis with nylon clamp associated to polymethylmethacrylate bone cement - experimental study in dogs cadavers

Fractures of iliac body are generally consequences of traumas of great impact. These fractures lead to a lack of locomotor response and in specific cases, surgery is needed. Among the possible therapeutic lines, implants become a good option. In this paper, the authors used dog corpses to develop a surgery technique of iliac osteosynthesis, employing nylon clamps and bone cement of polymethylmethacrylate (PMMA). Sixteen hemi pelvises of eight dog corpses, which had its iliac bodies totally fractured previously, were utilized. The osteosynthesis was reached through the implantation of six nylon clamps, distributed equally between the bone fragments. With the use of the nylon clamps there were bone realignment and a contact surface to fixation of the biomaterial. The technique developed in corpses was easily executed, effective in reduction, alignment and stabilization of the fracture. The authors suggest more studies in vitro to evaluate immobilization resistance and functionality in dogs with this condition.

Morfologia e expressão dos MRF’s no músculo esquelético de ratos submetidos a estímulo atrófico e recuperação por treinamento físico

Recent research advances in understanding the cellular and molecular mechanisms that underlie the processes of hypertrophy and atrophy. This may contribute to development of effective therapeutic strategies to attenuate or block the loss of muscle tissue associated with aging and pathological conditions. In this context, myogenic factors that control the activity of satellite cells have been studied to better understand the events involved in the recovery of muscle mass. Among them, we highlight the Myogenic Regulatory Factors (MRFs), which have been described as potential mediators of muscle growth. The objectives of this study evaluated the morphofunctional adaptations and gene expression of MRFs (MyoD and myogenin) in skeletal muscle (soleus) subjected to an atrophic stimulus followed by physical training. It was used 64 male Wistar rats (80 days, 250 to 300 g), divided into 8 groups (n = 8): C: control animals a week, I: Animals immobilized a week, C3: control animals 3 days; R3: Animals immobilized and recovered for 3 days, T3: Animals immobilized and submitted to exercise for 3 days; C7: Animals controls 7 days; R7: Animals immobilized and subsequently recovered by 7 days, T7: Animals immobilized and subsequently subjected to exercise for 7 days. Initially, the animals in groups I, R3, R7, T3 and T7, were submitted to 7 days of immobilization of the hind limb. Muscle atrophy was confirmed after a direct statistical comparison of the values of cross-sectional area (CSA) of muscle fibers studied in animals in groups I and C, sacrificed immediately after the immobilization period. Then, the groups T3 and T7 were submitted a rehabilitation program with muscle aerobic exercise (swimming) for 3 and 7 days respectively. The groups C, C3 and C7 were kept without stimulus atrophic and were not subjected to exercise. At the end of the experiment, the animals were sacrified and the soleus muscle removed. The quantitative analysis of gene expression ...

Considerações anestésicas para realização da cirurgia de facoemulsificação em cães

Cataract is the leading cause of blindness in dogs. It is defined as an opacity in the lens and is independent of resulting visual deficit. Although there are a growing number of studies related to drug therapy of cataract, the treatment is still considered exclusively surgical. Among the techniques used to remove cataract, phacoemulsification is the best performer. Anesthesia in intraocular surgery has several peculiarities, which are essential to successfully conduct phacoemulsification in dogs. In these patients, a safe and effective anesthesia requires the maintenance of intraocular pressure close to normal, preventing the activation of the oculo-cardiac reflex and complete immobilization of the eye. The appropriate conditions for such surgical procedures can be established through the use of drugs from different pharmacological groups for premedication, induction and maintenance of anesthesia, making a balanced anesthesia. This work was compiled from a careful review of the literature on anesthesia in cataract surgery. Considerations on the pre-medication, induction and maintenance of anesthesia are discussed, aiming to contribute to the phacoemulsification becomes more safe and suitable for the anesthesiologist and the surgeon as well as comfortable for dogs undergoing surgery

Trauma cranioencefálico em pequenos animais

Due to the importance of traumatic brain injury (TBI) in small animals, this paper aimed to discuss the pathophysiology of the TBI, the pre-hospital and hospital therapeutic procedures, as well as considerations related to transport and initial care of the patient immediately after trauma. The professional must identify the pre-hospital TBI and to treat the victim as a polytraumatized patient, including immobilization. In hospital therapy the procedures of craniotomy are important, especially to remove blood clots. In addition, the use of glucocorticoids must be avoided because of side effects, but combination therapies such as mannitol with furosemide increase the probability of success