Exposure to lard-based high-fat diet during fetal and lactation periods modifies breast cancer susceptibility in adulthood in rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diabetes mellitus activates fetal gene program and intensifies cardiac remodeling and oxidative stress in aged spontaneously hypertensive rats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Streptozotocin-Induced Diabetes Models: Pathophysiological Mechanisms and Fetal Outcomes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expression of 8-oxoguanine Glycosylase in Human Fetal Membranes

Problem The most common DNA lesion generated by oxidative stress (OS) is 7, 8-dihydro-8-oxoguanine (8-oxoG) whose excision repair is performed by 8-oxoguanine glycosylase (OGG1). We investigated OGG1 expression changes in fetal membranes from spontaneous preterm birth (PTB) and preterm premature rupture of the membranes (pPROM) and its changes in vitro in normal fetal membranes exposed to OS inducer water-soluble cigarette smoke extract (CSE). Method of study DNA damage was determined in amnion cells treated with CSE by comet and FLARE assays. OGG1 mRNA expression and localization in fetal membranes from clinical specimens and in normal term membranes exposed to CSE were examined by QRT-PCR and by immunohistochemistry. Results DNA strand and base damage was seen in amnion cells exposed to CSE. OGG1 expression was 2.5-fold higher in PTB samples compared with pPROM (P=0.045). No significant difference was seen between term and pPROM or PTB and term. CSE treatment showed a nonsignificant decrease in OGG1. OGG1 was localized to both amnion and chorion with less intense staining in pPROM and CSE-treated membranes. Conclusion Increased OS-induced DNA damage predominated by 8-oxoG is likely to persist in fetal cells due to reduced availability of base excision repair enzyme OGG1. This can likely lead to fetal cell senescence associated with some adverse pregnancy outcome.

Expression profiles of fetal membrane nicotinamide adenine dinucleotide phosphate oxidases (NOX) 2 and 3 differentiates spontaneous preterm birth and pPROM pathophysiologies

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Forskolin effect on the cryosurvival of in vitro-produced bovine embryos in the presence or absence of fetal calf serum

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparação da razão carga/deflexão entre fios ortodônticos de Ni-Ti termoativados 0,014”x0,025” e 0,016”x0,022”

The knowledge of the mechanical properties of nickel-titanium (NiTi) termoactives of the more accessible of the domestic market is still limited. Given this, the objective of this study was to evaluate and compare through deflection tests in brackets NiTi wires 03 term rectangular gauge 0.014 '' enabled x 0.025 '' and 0.016 '' x 0.022 '' of different brands (MORELLI (R), ORMCO (R) ORTHOSOURCE (R), ORTHOMETRIC (R), EURODONTO (R) and ADITEK (R)). All tests were carried out on universal testing machine EMIC DL 2000 under identical conditions and controlled at a temperature of 36 degrees C +/- 0.5 degrees C. Five measurements (N= 5) were performed for each thickness/wire tag that was deflected up to a limit of 4.0mm at a speed of 1.0mm/min. Each 0.2mm (round trip) of corresponding strength measured deflection for the construction of the graph of force x deflection at Tesc program version 3.04. Each graphic was evaluated according to the following variables: beginning of the Martensitic transformation (cN and mm), maximum strength (cN), the beginning and end of the plateau of deactivation (cN and mm) and length (mm) plateau. The average and standard deviation were calculated for all variables and statistical analysis was made by ANOVA tests 2 criteria and Turkey or Kruskal-Wallis and Dunn, a 5% level of significance. The results showed that the tests of 0.014 '' x0.025 '' ORTHOMETRIC (R) brands and ORMCO (R) showed the best results, as well as the wires of the MORELLI (R) and ORTHOSOURCE (R) to wires 0.016 '' x0.022 ''. In General, the gauge wires 0.014 '' x0.025 '' showed strength levels on the plateau of deactivation to 6 x smaller than 0.016 '' x0.022 '' caliber.

Fetal malformation in a marmoset (Callithrix jacchus): case report

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da atenção concentrada e memória em tabagistas com baixa carga tabágica

Introduction: Smoking is considered by World Health Organisation (WHO) a disease, since nicotine is addictive and cause physical, emotional and behavioral disorders in people who smoke. The objective of this study was to evaluate the concentrated attention and memory in smokers and nonsmokers, and the influence of one cigarette in this variables. Material and Methods: We selected two groups of subjects between 18 and 25 years, with no history of acute or chronic respiratory disease. Group 1 (G1) consist of 40 nonsmoking individuals and group 2 (G2) 40 smokers (who smoked less than 20 cigarettes per day, for up to 10 years). The subjects were tested for concentrated attention and memory test (AC Test), nonsmokers performed the test once, smokers performed the test twice, before smoking and after five minutes after smoking one cigarette. Smokers stayed without cigarret for one our before the first test. Results: Comparing the tests nonsmokers versus smokers first test, there was no significant difference (p> 0.05), however, the analysis of the two trials of smokers, showed improvement in concentrated attention and memory after smoke (p <0.0001). Conclusions: There was no difference in Concentrated Attention and Memory among nonsmokers and smokers in abstinence, however smokers showed significant improvement in AC Test after smoking.