Anatomia foliar com ênfase nos tricomas secretores e análise cromatográfica do óleo essencial de Melissa officinalis L. (Lamiaceae)

Adult leaves of Melissa officinalis L. (Lamiaceae) harvested during the time of budding had been used in the study of the leaf anatomy in the identification of the secretory structures. The material was fixed in FAA 50%, dehydrated in alcoholic series (ethyl), infiltrated in paraffin, sectioned at 13 mm, staining and later analyzed through optic microscopy. Also it was carried through a study in gaseous chromatography for attainment of the rude essential oil. Analyses of transversal sections of the leaf of Melissa officinalis, has identified the presence of two types of trichomes secretory: peltate and capitate, beyond the presence of trichome tector. Trichome capitate, identified in literature as (type I), presents variations in its morphology in relation to the cells number stalk and the head secretory cells number. The chromatographic analysis of the essential oil identified the presence of monoterpenes, in two major components, responsible for more than 87.8% of the relative composition in the rude oil, beyond sesquiterpenes in smaller proportions.

Detecção de pistas de aeroporto em imagens orbitais utilizando morfologia matemática

The Brazilian Cartography presents great deficiency in cartographic products updating. This form, Remote Sensins techniques together Digital Processing Images - DPI, are contributing to improve this problem. The Mathematical Morphology theory was used in this work. The principal function was the pruning operator. With its were extracted the interest features that can be used in cartographic process updating. The obtained results are positives and showed the use potential of mathematical morphology theory in cartography, mainly in updating.

Área foliar e evapotranspiração de callas cultivadas em substrato em função do nível freático

The objective of this study was to determine the influence of five different water levels on the crop development of Calla. The crop parameters evaluated were leaf area and evapotranspiration. The study was conducted in glass greenhouse with 50% of sunlight reduction. The plants were grown in PVC pots with 150 mm diameter, which were filled with substrate. The plant tubers weighed from 10 g to 12 g. The pots were placed within containers, under water level constant automatically.. The table water levels used were 10, 17, 24, 31 and 38 cm. Nine evaluations during the growth cycle checked the growth development. The evaporation varied from 26.89 to 46.14 L.plant-1 for 38 and 10 cm water levels, respectively and leaf area per plant showed 1011.6 to 2016.3 for the same levels. The substrate water was more available in the treatment 24 cm, with more restrictions in the upper and lower treatments. There was positive correlation between leaf area and evapotranspiration at the final of the culture.

Morfologia de plântulas e estádios juvenis de espécies invasoras

The morphology of 20 species of weeds that occur in the most common crops in Maringá, Paraná State, Brazil and adjacent regions were described at early developmental stages, in order to identify the species in the field and provide information for taxonomic and ecological works. Sixteen species featured epigeal-foliaceous seedlings. The seedling in Cardiospenuum halicacabum is epigeal-fleshy; in Cenchrus echinatus and Digitaria horizontalis it is hypogeal and in Commelina virginica is epigeal-cryptocotyledonar. The differentiation of the first eophyll occurred within ten (Digitaria horizontalis) to 35 days (Bidens pilosa) and the first metaphyll appeared within 22 (Commelina virginica) to 49 days (Acanthospenuum australe). The number of eophylls varied from one to four, and the phyllotaxis varied from alternate to opposite.

Morfologia e morfometria dos tipos de fibras do músculo reto abdominal de cadelas (canis familiaris) durante a gestação

The present paper deals with the evaluation of morphological and morphometric alterations of slow-twist (I) and fast-twitch (II) fibers of rectus abdominis muscle of adult female dog during pregestational phase, at 30 and 60 days of pregnancy and at 30 days after the parturition. At the every phases, using the open biopsy technique, muscle samples were collected. The samples were plunged. Histological sections were cut in a microtome. For general morphology, some sections were stained with HE. Subsequent sections were reacted for myofibrillar ATPase (m-ATPase), after alkaline (pH 10.4) and acid (pH 4.4) pre-incubations, in order to identificate type I and II fibers. In the pre-gestational phase, muscle tissue revealed to be composed by fibers with different diameters, presenting polygonal outlines and one or more periphery nuclei. At 30 days of pregnancy, muscle fiber characteristics were similar. At 60 days, in addition to the existence of normal fibers, polymorphic and small diameter fibers were frequent. At 30 days after the parturation, the morphology of muscle fiber were similar to that observed in the pre-gestational phase. In the four phases, type II fiber diameters were lager than type I. The diameters of both fiber types showed a significant reduction in the 30 days phase and a significative increasing at 60 days. The expansion of the abdominal wall during the pregnancy represents a chronic stimulus, induced changes in the morphology and in the fiber type diameters.

Morfologia do intestino delgado de capivara - Hydrochoerus hydrochaeris (Linnaeus, 1766)

The high potential for the exploitation of capybara requires information about its digestory morphophysiology, to improve nutritional handling. In the present study, gross anatomy, light microscopy and body and intestine lengths of 25 capybaras were evaluated. The minimum and maximum small intestine lengths for females and males were, respectively, 441 cm and 1734 cm, and 355 cm and 1123 cm. These values position the capybara between canine and swine intestinal lengths. The ratio between small intestine and body length was 12:1, without differences between sexes. There were no statistically significant differences between sexes for each part of small intestine. Correlation between length of each small intestine segment and body length was positive, and statistically significant only for the duodenum. The small intestine wall was formed by mucosa, submucosa, muscular and serosa. The mucosa presented intestinal and duodenal glands, of mucosal and serosal types, respectively. The mucosa muscular layer consisted of two distinct layers in the jejunum and ileum, and a thin and single layer in the duodenum. The submucosa, formed by moderate dense connective tissue, didn't show glands. The fiber bundles of the internal layer of muscular tunic were helicoidally arranged. The gross anatomy of the capybara small intestine was similar to canine and swine intestines. Microscopically, however, subtle differences can be identified in the submucosa and internal muscular tunics.

Tamanho da amostra foliar para avaliação do estado nutricional de goiabeiras com e sem irrigação

The ideal size precision of the foliar sample determines manual work optimization, and also diminishes inherent errors in diagnosis reports of nutritional state. This work aimed to determine the size of the foliar samples and the sample error variation in guava plantations submitted to two hydric cultivations for the nutritional state diagnosis of this fruit. The work included two studies, both under an entirely randomized experimental design. Study 1 was carried out in an orchard under unirrigated cultivation with four treatments and six repetitions that consisted of leaf collection in 5, 10, 20 and 40 plants. Study 2 was carried out in an orchard under irrigated cultivation with five treatments and 10 repetitions that consisted of leaf collection in 10, 20, 30, 40 and 50 guava plants. It was concluded that in unirrigated orchards it is necessary to sample leaves in 40 plants in order to keep the macronutrients sample error between 5 to 10%. For the micronutrients, on the other hand, at least 40 plants were necessary and, if Fe and Zn were considered, the sample must be even larger. In irrigated orchards, leaves deriving from 10 plants were enough to keep the sample error between 5 to 10%. However, considering the micronutrients, it was necessary to sample 20 guava plants.

Nitrogênio em cobertura e molibdênio via foliar em feijoeiro de inverno no sistema plantio direto II - Características agronômicas e produtividade

Nitrogen is the nutrient most uptaken by common bean plant, and molybdenum besides its importance to N fixation process, it is associated to N metabolism. The objective of this work was to evaluate, on common bean crop Pérola cultivar, the effect of N levels application at side dressing (0, 30, 60, 90 and 120 kg ha-1) as well as the leaf application of Mo (0, 80 and 160 g ha-1) at development stadium V3 or V4 under no tillage system. The experimental design was the randomized blocks with 30 treatments and 4 replications. The research was conducted in Selviria-MS on soil previously cropped with corn. The application of nitrogen levels at side dressing provided increasing on dry mass weight, number of pod and seeds per plant and grain yield, in the 2004; the Mo application at V3 or V4 stadia did not effect the production components and grain yield; as well as different doses of Mo depended on the N application at side dressing, in the 2004.

Fatores preditivos do sucesso da inseminação intrauterina: A morfologia espermática de acordo com o novo manual de laboratório da Organização Mundial de Saúde

Objective: The aim of our study was to assess the likelihood of IUI success as a function of the previously described predictive factors, including sperm morphology according to the new reference values defined by WHO. Material and Methods: This retrospective study enrolled 300 couples which underwent IUI. Regression analyses were used to correlate maternal age, number of preovulatory follicles on the day of hCG administration, number of inseminated motile sperm, and normal sperm morphology with clinical pregnancy. Results are expressed as odds ratio (OR) with 95% of confidence intervals (CI). Results: Women older than 35 years showed a lower pregnancy rate (6.5% vs 18.2%, p=0.017). Logistic regression models confirmed the lower chance of pregnancy occurrence for older women (OR: 0.39; CI: 0.16-0.96; p=0.040). The presence of two or more preovulatory follicles on the day of hCG administration resulted in higher pregnancy rate when compared to cases in which only one preovulatory follicle was present (18.6% vs 8.2%, p=0.011). The regression model showed a more than two fold increase on probability of pregnancy when two or more preovulatory follicles were detected (OR: 2.58; CI: 1.22-5.46, p=0.013). The number of inseminated motile sperm positively influenced pregnancy occurrence (OR: 1.47; CI: 0.88-3.14, p=0.027). Similar pregnancy rates were observed when semen samples were classified as having normal or abnormal morphology (10.6% vs 10.2%, p=0.936). Conclusion: Our results demonstrate that sperm morphological normalcy, according to the new reference value, has no predictive value on IUI outcomes. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida.

Morfologia de frutos e sementes e morfofunção de plântulas de Moringa (Moringa oleifera Lam.)

The objective of this work was to characterize external and internal morphologic aspects of the fruit, seed, and plant of Moringa oleifera Lam. The fruits were collected in the forest garden of Falculty of Agrarian and Veterinarian Sciences, São Paulo State University, Jaboticabal-SP, Brazil. It was observed that the Moringa fruits are brown simple darkness, dry of the type capsule, tends on average: 28.50 cm of length, 2.21 cm of width, 9.91 g of mass and with 12 seeds by fruit, it considerated fruits of medium and small size. The seeds are brown dark and they present three lines clear chestnut tree. The embryo is oleaginous, has a pair of cotyledons and the germination is hypogeal-cryptocotyledonary. The seed has1.037 cm of length and 1.0 cm of thickness of average and have a considerate medium and light weight (197g/1000 seeds). The germination began eight days after the planting and in the 25th day they left the primary leaves.

Aplicação de silicato de potássio e crescimento foliar da cana-de-açúcar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Morfologia de sementes e de estádios iniciais de plântulas de espécies de Bromeliaceae da Amazônia

Seeds of Aechmea bromeliifolia, A. castelnavii (Bromelioideae); Dyckia duckei, D. racemosa (Pitcairnioideae) and Tillandsia adpressiflora (Tillandsioideae) were collected in the Amazon regions (Mato Grosso) and studied to describe morphological characterization and post-seminal development, which can be taxonomically useful, and to assess percent germination. All the species have epigeous germination and produce cryptocotyledonary plantlets. Seeds have no dormancy and percent germination is high (over 86%), which facilitates the production of seedlings and conservation studies. Exclusive characteristics of the genera include: the seed coat of Aechmea (Bromelioideae) has mucilage that prevents desiccation; whereas that of Dyckia (Pitcairnioideae) has membranaceous wings and that of Tillandsia (Tillandsioideae) has feathery appendages, both of which make dispersal easier and establish the epiphytic habit. Initial post-seminal development of Aechmea (Bromelioideae) and Dyckia (Pitcairnioideae) is marked by the emergence of primary roots, interpreted as a basal character, whereas that of Tillandsia adpressiflora (Tillandsioideae) is marked by the emergence of the cotyledon, interpreted as a derived character. Dyckia and Tillandsia have a small tank only in the seedling phase while the contrary occurs in Aechmea.

Identification of foliar diseases in cotton crop

The pathogens manifestation in plantations are the largest cause of damage in several cultivars, which may cause increase of prices and loss of crop quality. This paper presents a method for automatic classification of cotton diseases through feature extraction of leaf symptoms from digital images. Wavelet transform energy has been used for feature extraction while Support Vector Machine has been used for classification. Five situations have been diagnosed, namely: Healthy crop, Ramularia disease, Bacterial Blight, Ascochyta Blight, and unspecified disease. © 2012 Taylor & Francis Group.

Propagação assexuada de chacrona (psychotria viridis ruiz & pavon) via estaquia foliar

This study aimed to assess the asexual propagation of Psychotria viridis by leaf cuttings. The treatments were: A - Whole leaf cuttings, B - leaf cuttings with the top third cut off; C - with the lower third of the cuttings removed; D - with cuts on the primary vein of leaf cuttings, and E - poles with cuts on the leaf's secondary veins. The cuttings were immersed in distilled water for a period of 70 days. In all the treatments, the rooting was observed to occur in the region of the cut, or the place where the incision in the vein was. Current assay shows the feasibilities of Psychotria viridis leaf stalks and concludes that cuttings at the nerve ends highlight rooting in so far as the leaf stalks remain in permanent contact with the plant.

Adubação nitrogenada e critérios de amostragem foliar para a cultura da batata

The present study aimed to determine which leave will be displayed and the time of collection of this leave. The treatments consisted on five doses of nitrogen, four times for collection sampling and three types of leaves, arranged in a randomized block design with three repetitions, totalizing one hundred and forty-four experimental units. The leaves collections took place every fifteen days at thirty, forty-five, sixty and seventy five days after budbreak (DAB), collecting the laminated / compound young leaf (first leave), newly mature (second leave) and the mature leave (third leave) from the set of terminal leaflets. For this was installed an experiment with the culture of potato (cv. Atlantic), in Barretos/SP, the period of march the june of 2010. According to this data collected in this study, the best time for collecting the leaves is at 30 days after the budbreak of the potato cultivation containing nitrogen concentrations in the leaves minus heterogeneous of plants from a fraction to another and with values R2 = 0.98 being higher to the all times of harvests. To the diagnostic leaf, the highest determination coefficient was observed in the newly mature (second leaf) with R2 = 0.98. It can be observed that the highest levels of nitrogen were found on the first leaf (39.01 kg-1). Nevertheless, the values were very heterogeneous and did not fit the curve being the second leave (newly mature) the one that best represents the nutritional status of the plant. Therefore the recommendation for nutritional diagnosis will collect the recently matured leaves (2nd leaf) 30 days after budbreak.