Composição química e digestibilidade in vitro da massa seca de cana-de-açúcar acrescida de ureia em diferentes tempos de estocagem

The objective of this research was to evaluate the chemical composition and in vitro dry mass (DM) digestibility of sugar cane with urea, maintained in the shade and sun, at different storage times. The utilized design was the completely randomized in a factorial scheme 6x2, that is, six storage times after the mixing (0; 2; 4; 6; 12 and 24 hours) and two storage location (shade and sun), with three replicates. The sugarcane utilized presented 12 months of development and was disintegrated for application of mixture (nine parts of urea for one of ammonium sulfate) to 1.0kg/100.0kg of fresh sugarcane. The samples taken with 12 hours of storage indicated that was an increase in the content of DM and crude protein (CP) of sugar cane compared to the moment of the mixture (307.6 vs. 294.2g/kg of DM and 115.2 vs. 99.3 g/kg of DM, respectively), and the smaller content of neutral detergent fiber (NDF) was observed around of 12 hours of storage (465.0g/kg of DM). The coefficients of in vitro dry mass digestibility (IVDMD) ranged of 0.558 to 0.612 in the times 0 and 12 hours, respectively. The length of storage changes the chemical composition of sugar cane plus urea. The storage location changes the dry mass content and pH values.

Fezes equina como fonte de inóculo na obtenção de indicadores indigestíveis para estimar a digestibilidade em equinos

Four castrated horses were utilized in randomized blocks experimental design. The objective was to study the in vitro acquirement of indigestible fiber, neutral detergent fiber (NDFi) and acid detergent fiber (ADFi), using as inoculum source rumen fluid (RF) or horse feces (HF), to estimate the nutrient digestibility (ND) in horses. Treatments consisted of direct method by total feces collection (TC) and indirect method either by the use of NDFi or ADF by the use of LR or HF as inocula source. The first essay was done with horses fed coast-cross hay exclusively, while for the essay two, the horses were fed of 70% of coast-cross hay and 30% corn grain. For the essay 1, ADFi-HF presented the best marker recuperation rate (RR) (103.67%), being similar to control (TC), while NDFi-RF and ADFi-RF resulted in lower RR (P<0.05), 83.43 and 88.28%, respectively. The ND was adequately estimated by NDFi-HF and ADFi-HF. On the second essay there were no significant effects of the marker type, as well as for the indigestible marker acquirement method, for the marker RR (average value of 101%). The ND was adequately predicted by the ADFi obtained for both innocula source and NDFi obtained by the use of HF as inoculum, for horses fed a mixed diet. It can be concluded that horse feces may be used as inoculum source for the acquirement of NDFi and ADFi in vitro for digestibility determination on horses fed coastcross hay exclusively, as well as for horses fed a mixed diet.

Determinação bromatológica de pleurotus ostreatus cultivado em resíduos de diferentes cultivares de bananeira

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fontes energéticas associadas ao farelo de girassol ou à ureia em dietas para novilhos

The objective of this study was to evaluate the performance and carcass traits of steers fed corn or citrus pulp associated with sunflower meal or urea. Diets were composed of two energy sources (corn grain and citrus pulp) associated with two protein sources (soybean meal and urea), resulting in four treatments in forage:concentrate ratio of 40:60. It were used 24 steers, 1/2 Angus × 1/2 Nellore (18 months old and 329 kg BW) distributed in a completely randomized design which were finished in feedlot for 80 days. It were evaluated the intake, average daily gain and carcass traits of the animals. Intake of dry matter (DMI), crude protein (CPI) and neutral detergent fiber (NDFI) were affected (p<0.05) by diets. The diet with citrus pulp and urea (PU) showed the lowest (p<0.05) DMI (8.30 kg), CPI (1.03 kg) and NDFI (3.31 kg). The average daily weight gain (ADG) followed the behavior of DMI, NDFI and CPI and the lowest value (p<0.05) was observed for animals fed diet PU. Regarding carcass traits, diets containing corn associated with urea (MU) or sunflower meal (MFG) and citrus pulp associated with sunflower meal (PFG), provided the highest (p<0.05) carcass cold weights (238.37, 247.26 and 237.14 kg, respectively). However, the lowest cold carcass dressing percentage (p<0.05) was obtained in diet MU (50.57%). The association of citrus pulp with urea should not be performed when citrus pulp partially or totally replace corn as energy source in diets, because possibly this association promote an energy deficit resulting in lower performance and worse carcass traits of animals finished in feedlot.

Composição química e degradabilidade ruminal de silagens da cana-de-açúcar tratada com aditivos químicos e bacteriano1

The objective of this trial was to evaluate the effect of the addition of chemical and bacterial additive in the ensiling of sugar cane (Saccharum officinarum L.) on chemical composition, pH, kinectic fraction and in situ degradation of nutritions components of silages. Five rumen-cannulated 1/2 Simental + 1/2 Zebu steers were allotted to a completely randomized design. The steers were placed in individual cages and they were fed with diets with 76% forage (%DM). Five silages were evaluated: control - sugar cane, no additives; urea - sugar cane + 0.5% of urea (wet basis); inoculant - sugar cane inoculated with LactoSilo® (390 g/40 t forage); NaOH - sugar cane + 1.0% of sodium hydroxide (wet basis); CaOH - sugar cane + 0.6% of calcium hydroxide (wet basis). The silage additives with sodium hydroxide showed the highest pH values before (11.20) and after (4.87) for silage. No differences were observed among the silages for dry matter (26.85), crude protein (5.25) and acid detergent fiber (57.21). Fractionation of dry matter and organic matter of silages showed similar behavior, with higher values of the soluble fraction (fraction A) for silages with sodium hydroxide (45.86 and 30.95%) and calcium hydroxide (29.47 and 26.13%). The use of sodium hydroxide allowed obtaining higher values for the degradation of cell wall components of silages from cane sugar. The potencial and effective degradability with 3, 5 and 8%/h of passage rate were respectively 88.44, 64.45, 56.73 and 49.83% for NDF and 82.57, 55.51, 46.72 and 38.83% for ADF, indicating that the use of sodium hydroxide as chemical additives can improve the nutritive value of cane sugar silage.

Substituição dos grãos secos de milho pela silagem de grãos úmidos de milho para vacas da raça holandesa em lactação

In order to evaluate the effects of replacement of dried corn (GSM) for silage moisture corn (HMCS) on production and milk composition were used five Holstein cows, primiparous with a mean of 112 days post delivery, confined in Tie Stall, for 70 days. We used five diets according to NRC to 17.5% CP (DM) and 2.4 Mcal / kg DM, the 40:60 ratio of roughage and concentrate containing soybean meal, sugar cane silage and hay and substitution levels of the GSM HMCS the following treatments: a) 0%, 2) 25%, 3) 50%, 4) 75% and 5) 100%. Milk production and dry matter intake (DMI) were recorded daily. The animals were milked daily 6:00 and 18:00 h, and milk samples collected from consecutive milkings of each experimental period of 14 days (four days of collection). The experimental design was a 5x5 Latin square and the data analyzed by the statistical program SAS. Body weight (508 kg), milk (23.6 kg), corrected milk (22.7 kg), DMI (17.13 kg) showed no significant difference, but the intakes of neutral detergent fiber (6.67 kg), and detergent acid (3.39 kg), feed efficiency for the production of milk (milk 1.41 kg / day) urea nitrogen (17.67 mg / dL) differ, thus indicating that HMCS is more efficient than GSM in the diet of dairy cows not alter the production and milk composition.

Characterization of a glucose- and solvent-tolerant extracellular tannase from Aspergillus phoenicis

Tannases have attracted wider attention because of their biotechnological potential, especially enzymes from filamentous fungi and other microorganisms. However, the biodiversity of these microorganisms has been poorly explored, and few strains were identified for tannase production and characterization. This article describes the production, purification and characterization of a glucose- and solvent-tolerant extracellular tannase from Aspergillus phoenicis. High enzymatic levels were obtained in Khanna medium containing tannic acid up to 72 h at 30 °C under 100 rpm. The purified enzyme with 65% of carbohydrate content had an apparent native molecular mass of 218 kDa with subunits of 120 kDa and 93 kDa and was stable at 50 °C for 1 h. Optima of temperature and pH were 60 °C and 5.0-6.5, respectively. The enzyme was not affected significantly by most ions, detergents and organic solvents. While glucose did not affect the tannase activity, the addition of a high concentration of gallic acid did. The Km values were 1.7 mM (tannic acid), 14.3 mM (methyl-gallate) and 0.6 mM (propyl-gallate). The enzyme was able to catalyze the transesterification reaction to produce propyl-gallate. All biochemical properties suggest the biotechnological potential of the glucose- and solvent-tolerant tannase from A. phoenicis. © 2012 Elsevier B.V. All rights reserved.

Immobilization and stabilization of a bimolecular aggregate of the lipase from Pseudomonas fluorescens by multipoint covalent attachment

The soluble lipase from Pseudomonas fluorescens (PFL) forms bimolecular aggregates in which the hydrophobic active centers of the enzyme monomers are in close contact. This bimolecular aggregate could be immobilized by multipoint covalent linkages on glyoxyl supports at pH 8.5. The monomer of PFL obtained by incubation of the soluble enzyme in the presence of detergent (0.5% TRITON X-100) could not be immobilized under these conditions. The bimolecular aggregate has two amino terminal residues in the same plane. A further incubation of the immobilized derivative under more alkaline conditions (e.g., pH 10.5) allows a further multipoint attachment of lysine (Lys) residues located in the same plane as the amino terminal residues. Monomeric PFL was immobilized at pH 10.5 in the presence of 0.5% TRITON X-100. The properties of both PFL derivatives were compared. In general, the bimolecular derivatives were more active, more selective and more stable both in water and in organic solvents than the monomolecular ones. The bimolecular derivative showed twice the activity and a much higher selectivity (100 versus 20) for the hydrolysis of R,S-2-hydroxy-4-phenylbutyric acid ethyl ester (HPBEt) in aqueous media at pH 5.0 compared to the monomeric derivative. In experiments measuring thermal inactivation at 75 °C, the bimolecular derivative was 5-fold more stable than the monomeric derivative (and 50-fold more stable than a one-point covalently immobilized PFL derivative), and it had a half-life greater than 4 h. In organic solvents (cyclohexane and tert-amyl alcohol), the bimolecular derivative was much more stable and more active than the monomeric derivative in catalyzing the transesterification of olive oil with benzyl alcohol. © 2012 Elsevier Ltd. All rights reserved.

Efficacy of mouth rinses and toothpaste on tooth whitening.

People increasingly desire tooth whitening. Considering the wide range of whitening products on the market, this study evaluated the efficacy of whitening toothpastes and mouth rinses compared with the 10% carbamide peroxide (CP) whitening gel. We obtained 120 cylindrical specimens from bovine teeth, which were darkened for 24 hours in a coffee solution. The color measurement was performed by a spectrophotometer using the CIE L*a*b* system, and specimens were divided into six groups according to the use of the following agents: group 1, conventional fluoridated toothpaste; group 2, Close Up White Now; group 3, Listerine Whitening; group 4, Colgate Plax Whitening; group 5, experimental mouth rinse with Plasdone; and group 6, 10% CP Whiteness Perfect. After the simulation of 12 weeks of treatment for groups 1 to 5 and 14 days of treatment for group 6, the specimens were subjected to a new color reading. Data were subjected to one-way analysis of variance (α=0.05), which showed significant differences among groups after 12 weeks for ΔE (p=0.001). Results of the Tukey test revealed that groups 3, 4, and 6 presented significantly higher color alteration than groups 1, 2, and 5. The whitening toothpaste Close Up White Now and the experimental mouth rinse with Plasdone showed similar color alteration as conventional toothpaste after a 12-week treatment simulation. These groups presented significantly lower color alteration compared with whitening mouth rinses Listerine and Colgate Plax Whitening, which showed similar results to those observed after 14 days of bleaching with 10% CP treatment.

Nitrogênio e idade de corte na qualidade da biomassa de capimelefante para fins agroenergéticos cultivado em Latossolo

Nowadays one of the major challenge for research is the production of alternative agrifuels energy material derived from plant biomass. Allied to this, the proper management of nitrogen, becomes fundamental for productivity gains and improvement in quality of elephant grass biomass. Thus, the objective was to evaluate the effect of nitrogen fertilization and plant age effect on the quality of elephant grass biomass for energy purposes. The experiment was performed with genotype Paraíso, grown in a dystrophic Oxisol in the southern state of Tocantins. The design was randomized blocks with subdivided plots, situating plots as nitrogen rates (0, 50, 100 and 150 kg ha-1) and the subplots cutting age (120, 150 and 180 days after planting ) four replicates. We evaluated the relationship carbon/nitrogen (C:N) and leaf/stem (L:S), levels of neutral detergent fiber (NDF) and ash contents in dry biomass produced. The application of nitrogen increased by 12% to C:N ratio of the plant. The advanced age of cut forage was increased 9% in the C:N plant, however, decreased by 12 and 14% levels of ash produced in the stem and leaves, respectively. The production of elephant grass for agrifuels is feasible with management system proposed, quality characteristics desirable, such as high C:N ratio, above 40% and higt fiber content, above 50%.

Consumo e digestibilidade aparente dos nutrientes em ovinos alimentados com dietas contendo diferentes quantidades de farelo de linhaça

The aim of this research was to evaluate the intake and apparent digestibility of nutrients in sheep fed with diets containing different amounts of linseed meal. The treatments evaluated were: corn silage exclusive (control); corn silage + concentrate; corn silage + concentrate + 5% of linseed meal; corn silage + concentrate + 10% of linseed meal. The dry matter (DM) intake has increased in diets containing concentrated if compared to diet exclusively with corn silage (P<0.05), however, there was no effect of the inclusion of linseed meal in diets (0; 5 and 10%). As it was expected, the diets containing 5 and 10% of linseed meal resulted in an increase of ether extract (EE) intake. A higher apparent digestibility of DM, organic matter (OM) and neutral detergent fiber (NDF) in the animals fed with diets containing the concentrate was observed, independently of linseed meal proportion. According to the experimental conditions under which the experiment was developed, itis recommended to provide the diets containing until 5% of linseed meal, because there is no negative effect on dry matter and nutrients intake, as well as on apparent digestibility.

Desempenho e características de carcaça de cordeiros alimentados com fontes de carboidratos associadas ao óleo de girassol

This work was carried out with the objective to evaluate the effect of different carbohydrate sources associated with sunflower oil on performance, carcass characteristics and cuts yields of feedlot sheep. Twenty four lambs were assigned to a completely randomized design with a 2 × 2 factorial arrangement with two sources of neutral detergent soluble carbohydrate, starch or neutral detergent soluble fiber, with and without the inclusion of 4.2% sunflower oil. The treatments consisted of diets containing high content of soluble fiber (17.14% DM) without adding oil, high in soluble fiber (16.35% DM) with inclusion of 4.2% sunflower oil, high starch (30.14% DM) without adding oil and high starch (28.21% DM) with addition of 4.2% of sunflower oil. The animals were feedlot with average initial of 17.7 kg and when reached 35 kg body weight were slaughtered. The different sources of carbohydrate and oil inclusion in the diet not influence the days on feedlot in the dry matter intake and average daily weight gain, but higher feed conversion was observed for the diet with a high percentage of neutral detergent soluble fiber compared to diet high in starch. The carcass weights and yields as well as the commercial cuts were not affected by diets. The performance and quantitative characteristics and carcass cuts of lamb are not affected by different carbohydrate sources and their association with 4.2% sunflower oil.

Production, purification, and characterization of a major penicillium glabrum xylanase using brewer's spent grain as substrate

In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+ and the reducing agents β-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+ as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost. © 2013 Adriana Knob et al.

Development of equations to estimate microbial contamination in ruminal incubation residues of forage produced under tropical conditions using 15N as a label1

The objective of this study was to use 15N to label microbial cells to allow development of equations for estimating the microbial contamination in ruminal in situ incubation residues of forage produced under tropical conditions. A total of 24 tropical forages were ruminal incubated in 3 steers at 3 separate times. To determine microbial contamination of the incubated residues, ruminal bacteria were labeled with 15N by continuous intraruminal infusion 60 h before the first incubation and continued until the last day of incubation. Ruminal digesta was collected for the isolation of bacteria before the first infusion of 15N on adaptation period and after the infusion of 15N on collection period. To determine the microbial contamination of CP fractions, restricted models were compared with the full model using the model identity test. A value of the corrected fraction A was estimated from the corresponding noncorrected fraction by this equation: Corrected A fraction (ACPC) = 1.99286 + 0.98256 × A fraction without correction (ACPWC). The corrected fraction B was estimated from the corresponding noncorrected fraction and from CP, NDF, neutral detergent insoluble protein (NDIP), and indigestible NDF (iNDF) using the equation corrected B fraction (BCPC) = -17.2181 - 0.0344 × fraction B without correction (BCPWC) + 0.65433 × CP + 1.03787 × NDF + 2.66010 × NDIP - 0.85979 × iNDF. The corrected degradation rate of B fraction (kd)was estimated using the equation corrected degradation rate of B fraction (kdCPC) = 0.04667 + 0.35139 × degradation rate of B fraction without correction (kdCPWC) + 0.0020 × CP - 0.00055839 × NDF - 0.00336 × NDIP + 0.00075089 × iNDF. This equation was obtained to estimate the contamination using CP of the feeds: %C = 79.21 × (1 - e-0.0555t) × e-0.0874CP. It was concluded that A and B fractions and kd of CP could be highly biased by microbial CP contamination, and therefore these corrected values could be obtained mathematically, replacing the use of microbial markers. The percentage of contamination and the corrected apparent degradability of CP could be obtained from values of CP and time of incubation for each feed, which could reduce cost and labor involved when using 15N. © 2013 American Society of Animal Science. All rights reserved.

Biofertilização na produção de alfafa e atributos

Pós-graduação em Ciência Animal - FMVA