Toll-Like recepctors (TLRs) and retinoic acid inducible gene – I (RIG-I) activation by viral analogs in bovine endometrial cells

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Morfologia do útero e ovários de Artibeus planirostris (Phyllostomidae: Chiroptera) durante as fases do ciclo reprodutivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da cavidade uterina com histerossonografia, uma revisão

Sonohysterography was firstly described three decades ago. The saline solution infusion into the uterine cavity favors its use and provides excellent visualization of the anatomy and the inner cavity of the uterus better than the conventional transvaginal sonography To check the current role of sonohysterography in the uterine cavity assessment in women with abnormal uterine bleeding and asymptomatic, a literature review comparing sonohysterograph with conventional transvaginal sonography and/or ambulatory diagnostic hysteroscopy was carried out. To this end, relevant studies were researched in electronic databases Medline/PubMed, SciELO/LILACS. The sonohysterography is an ambulatory procedure, non-invasive, better cost-benefit, better sensitivity and specificity to identify uterine abnormalities, causing minimal discomfort and low complications rate. It was subject to revision which there is no more doubt about its accuracy. It can be concluded that the sonohysterography is a useful tool in the propedeutics to assess uterine cavity of symptomatic patients with abnormal uterine bleeding, infertility, recurrent miscarriages, and embryonic implantation failures in assisted reproduction treatment / in vitro fertilization and in any other intra and extra uterine cavity alteration. Hence, conventional transvaginal sonography is indicated as an initial method of assessment of the uterine cavity previously to ambulatory diagnostic hysteroscopy.

Reproductive physiology, and physical and sexual development of female offspring born to diabetic dams

Objectives: The objective of this study was to evaluate physical and sexual development and reproductive physiology in female rat offspring that developed in hyperglycemia conditions in utero and during lactation. Materials and methods: Maternal diabetes was induced in female rats by a single IV injection of streptozotocin before mating. Female offspring development was evaluated by means of the following parameters: physical development; age of vaginal opening and first estrus; weight and histological evaluation of uterus and ovaries; duration of the estrous cycle, sexual behavior, and fertility after natural mating. Results: In the female offspring, maternal diabetes caused delays in initial physical development; diminution in ovary weight and number of follicles; and inferior reproductive performance compared with the control group. Conclusions: The exposure to hyperglycemia in uterus and during lactation caused delays in physical and sexual development, and affected the reproductive physiology of female rats negatively.

Potential endocrinedisruptor activity of drinking water samples

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Coinfection by avirulent Rhodococcus equi and Klebsiella oxytoca as a cause of atypical abortion in a thoroughbred mare

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito do benzoato de estradiol na recuperação de oócitos, embriões e nos parâmetros hematológicos de cadelas recém acasaladas

We investigated estradiol benzoate effects on oocytes/embryos recovery rate and the influence of this drug on the hematopoietic system. Twenty four bitches were divided in two groups, Group I, 12 females that received a single shot of estradiol benzoate, 0.2 mg/kg intramuscularly, between 2 and 7 days after the date of the last mismating or insemination and, Group II (control), 12 bitches that received 0.2 ml/kg of oily diluent, in corresponding dates. The bitches were ovary-hysterectomized and the uterus/oviduct were isolated and flushed with a PBS, heparin and polyvinyl alcohol solution. Oocytes and embryos were quantified and classified according to their stage of development. Blood counts were performed on M1 (before drug administration), M2 (15 days after drug administration) and M3 (40 days after drug administration). Pearson correlation coefficient was used to analyze the variable retrieval structures, while Fisher exact test was used for the analysis of embryonic viability. ANOVA was used to analyze repeated measurements and Tukey test for hematological parameters. All tests were performed at 5% significance level. The recovery rate of total structures in group I was lower (22.88%) than group II (65.85%). A lower embryo recovery (ratio 3: 52) rate and a greater number of degenerated structures (ratio 11: 1) were observed in group I. Hematological parameters showed significant difference in erythrocytes, hematocrit and hemoglobin concentrations 15 days after drug administration and difference in leukocytes concentration 40 days after using the medication in bitches of group I, however, at the end of the experiment all bitches had blood counts considered normal.

Infecção por strigea falconis em buteo magnirostris no Brasil

Background: Buteo magnirostris, popularly known as roadside hawk belongs to the family Accipitridae, Ciconiiformes. The specimen is common throughout Brazil inhabiting open areas, tolerating disturbed areas very well, but avoiding dense forests. The trematodes are common parasites in the intestines of birds of prey, with scant notice of pathogenic infections. However, severe infections of trematodes Strigeidae family have been previously reported as a cause of anemia, diarrhea, enteritis, weight loss and death. This paper aims to report the occurrence of infection in S. Falconis in B. magnirostris diagnosed by post mortem examination. Case: The specimen of B. magnirostris, male, young was sent for necropsy at the Laboratory of Veterinary Pathology, Federal Rural University of Semi-Arid (UFERSA), Mossoró-RN, Brazil. With a history of apathy, anorexia, diarrhea and death in one course of 24 h. The free bird life and even puppy had been captured for training and practice of falconry shortly before the clinical manifestation of infection (time of captivity uninformed). On physical examination ruffled feathers, cachexia and pallor of skeletal muscle was observed. At necropsy there was severe enteritis with petechiae and accumulation of liquid contents into the duodenum. Fifty-two trematodes were found set in duodenal mucosa. The other organs and structures showed no changes. Fragments of all organs were harvested, fixed in 10% formalin buffered, routinely processed for histopathology and stained with hematoxylin and eosin (HE). Parasites were carefully collected, washed in saline, fixed, processed and identified according to the morphology and taxonomy. Histologically, the lesions were restricted to the duodenum and were characterized by melting, severe atrophy and necrosis of the epithelial cells of the intestinal villi; inflammatory infiltrate (consisting of lymphocytes, plasma cells and eosinophils) in the lamina propria, in addition to trematodes infiltrated the mucosa and lamina propria. These were 60-80 mm in diameter, consisted of parenchymal body enclosed by the integument. In some cross sections of the parasite was possible to observe the presence of cecum, testis and uterus, with some variations between sections; there were also yellowish eggs and coated with a delicate membrane. The trematodes contain approximately 1 mm in length and used as morphology and taxonomy has been identified as S. Falconis. Discussion: S. Falconis is a trematode intestinal parasite of birds of prey, with reports of its occurrence in Europe, North America and Central. In neotropical regions is described the occurrence of the subspecies S. Falconis brasiliana. Although the absence of clinical signs is a common pattern, parasitism by trematodes may become evident, common to captivity stress conditions, and thus infections, even for low pathogenic parasites can cause diarrhea, anorexia, weight loss and death, as reported in this paper. A factor that possibly contributed to the scant notice is its small size, which makes the observation of this parasite in analysis of necropsy in non-pathological conditions and also not familiar with the technical laboratories in the morphological shape of the eggs, which creates difficulty in finding the parasite in parasitological analysis in captive animals. Despite being considered poorly pathogenic trematodes, epidemiologically, the presence of the parasite should be considered a health risk to free-living predators, newly captive in parks, zoos, veterinary hospitals, triage center for wildlife and creators, as they may express pathogenicity in immunosuppressed animals. This work contributes to recording the presence S. falconis parasitizing the duodenal mucosa of B. magnirostris in Brazil.

Atuação do interferon-tau no reconhecimento materno da gestação

Maternal recognition of pregnancy is a process by which the conceptus signals its presence to the mother in order to prolong the life of the corpus luteum (CL) thus maintaining the pregnancy. This process occurs between days 15 and 19 after fertilization and is the most important biological challenge for obtaining satisfactory reproductive indices in bovine. Interferon-tau (IFN-τ) glycoprotein -secreted in the uterus by the conceptus- has a paracrine action inhibiting the expression of estrogen receptors (ESR1) and oxytocin (OXTR) in the endometrium, thus preventing the release of luteolytic pulses of prostaglandin F2 alpha (PGF2α), hormone responsible for the onset of luteolysis. IFN-τ also increases the expression of several interferon-stimulated genes (ISGs) in the uterus, CL, and blood cells. Direct endocrine action of IFN-τ on extrauterine tissues stimulates ISGs expression, which in the corpus luteum seems to be involved with luteal resistance to luteolytic action of PGF2α. This review discusses recent findings on the luteolysis mechanism in the bovine and endocrine and paracrine mechanisms such as IFN-τ during the maternal recognition of pregnancy.

Apoptose em placentônios bovinos de gestações de conceptos naturais e de transgênicos clonados

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Efeitos do acetato de deslorelina sobre a produção in vivo e in vitro de embriões de gatas domésticas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)