Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Massa de forragem, composição química do capim-massai e comportamento ingestivo de ovinos durante a fase de implantação do eucalipto em sistema silvipastoril

Pós-graduação em Zootecnia - FCAV

Efeitos do exercício físico de salto sobre as proteínas relacionadas à captação de glicose e via proteolítica dependente de ATP no músculo gastrocnêmio de ratos diabéticos induzidos por administração de aloxana

Pós-graduação em Fisioterapia - FCT

Perfil de ácidos graxos e análise sensorial de carne e iogurte de leite de caprinos alimentados com óleos vegetais

Pós-graduação em Zootecnia - FMVZ

Phosphorus reduction by sifting fish waste meal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Incorporação do carbono13 nos tecidos muscular e hepático de frangos de corte de diferentes grupos genéticos criados em estresse cíclico de calor

Pós-graduação em Zootecnia - FCAV

Characterization of peroxidase changes in tolerant and susceptible soybeans challenged by soybean aphid (Hemiptera: Aphididae)

Changes in protein content, peroxidase activity, and isozyme profiles in response to soybean aphid feeding were documented at V1 (fully developed leaves at unifoliate node, first trifoliate leaf unrolled) and V3 (fully developed leaf at second trifoliate node, third trifoliate leaf unrolled) stages of soybean aphid-tolerant (KS4202) and -susceptible (SD76R) soybeans. Protein content was similar between infested and control V1 and V3 stage plants for both KS4202 and SD76R at 6, 16, and 22 d after aphid introduction. Enzyme kinetics studies documented that control and aphid-infested KS4202 V1 stage and SD76R V1 and V3 stages had similar levels of peroxidase activity at the three time points evaluated. In contrast, KS4202 aphid-infested plants at the V3 stage had significantly higher peroxidase activity levels than control plants at 6 and 22 d after aphid introduction. The differences in peroxidase activity observed between infested and control V3 stage KS4202 plants at these two time points suggest that peroxidases may be playing multiple roles in the tolerant plant. Native gels stained for peroxidase were able to detect differences in the isozyme profiles of aphid-infested and control plants for both KS4202 and SD76R.

Valor nutricional de silagem de capim-mombaça com aditivos agroindustriais

It was aimed to evaluate the chemical composition of grass silage-mombasa associated with different additives in four times of opening the silo. The experiment was conducted in UFGD. After harvesting the forage, biomass in natura crushed, was taken to the lab, homogenized and enriched on the basis of natural mass, with the following additives: 5% wheat bran, 5% of waste (broken grain and soy ice cream cone) of soybean, 5% urea in natural matter and the witness (without additive). The silos were opened after (unprocessed material), 15, 30 and 45 days, for the analysis of chemical composition. The data obtained were analyzed through the statistical programme SISVAR and averages were compared to 5% of probability, by Skott-Knot. The grass silage-mombasa without additive presented major (P<0.05) dry matter content compared to other treatments at time 0 and 30 days of silage and did not differ (P>0.05) of grass silage- mombasa associated with 5 of urea in 15 days and 45 of silage. The grass silage-mombasa with 5% urea showed the highest crude protein content at time 0 and differed from other treatments. The silage of mombasa associated with 5% urea provided greater in vitro digestibility of dry matter to 15 days of silage.

INTERCROPPING OF BRAQUIARIA WITH SOYBEAN

The objective of this work was to study the intercropping of Brachiaria brizantha. Marandu with soybeans. The experiment has been planted in a 3 year prevailing area with no-tillage, in eutrophic Oxisol at Maripa - PR. The experimental design was a randomized block with five replications. For the forage study, four treatments were performed which consisted of seeding times brachiaria [early ( seven days before planting soybeans) joint (same day of soybean planting) and after (at stages V-3 and R-1 culture)] intercropping with soybean. To study the yield and crude protein and oil levels of the grain were adopted six treatments, which consisted of sowing dates of capim Marandu [early (seven days before planting soybeans), joint (same day of soybean planting) after (V-3, R-1 and R-5 soybean) and in single culture (single)]. The forager higher productivity of dry matter occurs with early sowing, however, the greatest reduction in soybean yield also occurs in this case. The sowing of Brachiaria until R-5 soy has no influence on the nutritional value of the forage. The intercropped of soybean with Brachiaria brizantha. Marandu has no impact on the leaf Nitrogen content, oil content and enzyme activity of soybean peroxidase. The highest yield of soybeans occurs when it is grown single or Brachiaria is sown in stage R-5. The crude protein content of soybean grain is reduced when brachiaria seeding is early or together with soybeans compared to single culture.

Antioxidantes e biomarcadores da injúria oxidativa na saliva de pacientes com síndrome de down

Vulnerability of patients with Down syndrome (DS) to oxidative stress and damage has been attributed to the overexpression of the superoxide dismutase gene, which is located in the triplicated critical region 21q22.2 of chromosome 21. The objective of this study was to investigate enzymatic and non-enzymatic antioxidant systems and levels of biomarkers of oxidative damage in saliva of patients with DS. Saliva samples were collected from 30 patients with DS and 30 controls, ranging in age from 14 to 24 years. The following parameters were analyzed: superoxide dismutase activity, concentration of malondialdehyde, carbonylated proteins, uric acid, vitamin C and total protein, peroxidase activity, and total antioxidant capacity. Patients with DS presented significantly higher superoxide dismutase activity and malondialdehyde levels than controls (p<0.05). On the other hand, no difference in carbonylated proteins or antioxidants (uric acid, vitamin C, peroxidase, and total antioxidant capacity) was observed between DS patients and controls (p>0.05). Patients with DS also presented higher salivary total protein content (p<0.05). In conclusion, despite similar antioxidant levels patients with DS are more vulnerable to oxidative stress in saliva as indicated by a significant increase in malondialdehyde concentration and superoxide dismutase activity

Introdução de Lentinulaedodes (Shiitake) e Pleurotusostreatus (Shimeji) em bagaço de cana termo explodido para diminuição da toxicidade

Ethanol production has gained great prominence in the investment new renewable energy sources and Brazil is among the leaders of production. However, this activity generates large amounts of waste being the largest volume of the sugar cane bagasse. For this reason looking up ways to use this material as burning for energy production and composition of forage in the diet of ruminants, however there are difficulties to use this production for this last one. This paper proposes a microbiological treatment with Lentinula edodes and Pleurotus ostreatus in order to enable the bagasse in ruminant feed composition in order to be used more noble than their burning. After treatment with the fungus, tests were performed for quantifying crude protein by the method of Kjeldhal. It was verified that the protein content in the pure bagasse was 1.0% after fermentation the protein content was 4.2% with L.edodes and 4.9% with P. ostreatus. To evaluate the protein quality of the product fermented by L. edodes and P. ostreatus was applied microbiological method for growth of Enterococcus zimogenes verifying that after fermentation the protein quality was 76 and 27.4% with L. edodes and P.ostreatus, respectively, compared with casein. The quantification of amino acids showed significant improvement of protein with altered amino acid profile with treatments of fungos. About of DQO and BOD were also found considerable improvement besides considerable drop in toxicity as measured by acute toxicity test with Daphinia similis

Determinação da temperatura para inativação da urease em amostras de casca e farelo de soja

s not degraded in the rumen but destroy ureasis. Soybean meal is one of the protein ingredients commonly used in formulations of animal feeds. In the diets of monogastric bran has high protein content provided by a greater separation of soybean hulls. For the ruminant protein value of the meal is lower with the inclusion of soybean hulls to reduce the level of protein. With the increased consumption of meal high protein, due to increases in production of pigs and poultry in recent years, increasing the availability of soybean hulls on the market, this is because soybean hulls is little understood in the feeding of monogastric . In this context, the main objective of this study was to determine the times and temperatures needed to ensure the effective inactivation of ureasis present in the bran and soybean hulls. According to the assessments, to make the determination of temperatures and times required for the inactivation of ureasis present in the bran and soybean hulls are 170 º C and 25 minutes for soybean meal and 140 ° C 10 minutes for soybean hulls, respectively

Manejo da adubação nitrogenada na cultura do milho pelo uso da inoculação com azospirillum brasilense em consórcio com capim xaraés

Pós-graduação em Agronomia - FEIS

Periodontal disease-associated compensatory expression of osteoprotegerin is lost in type 1 diabetes mellitus and correlates with alveolar bone destruction by regulating osteoclastogenesis

Alveolar bone resorption results from the inflammatory response to periodontal pathogens. Systemic diseases that affect the host response, such as type 1 diabetes mellitus (DM1), can potentiate the severity of periodontal disease (PD) and accelerate bone resorption. However, the biological mechanisms by which DM1 modulates PD are not fully understood. The aim of this study was to determine the influence of DM1 on alveolar bone resorption and to evaluate the role of receptor activator of nuclear factor-kappaB ligand (RANKL)/osteoprotegerin (OPG) in osteoclastogenesis in rats. PD was induced by means of ligature in nondiabetic and in streptozotocyn-induced DM1 rats. Morphological and morphometric analyses, stereology and osteoclast counting were performed. RANKL and OPG mRNA levels, protein content, and location were determined. PD caused alveolar bone resorption, increased the number of osteoclasts in the alveolar bone crest and also promoted changes in RANKL/OPG mRNA expression. DM1 alone showed alveolar bone destruction and an increased number of osteoclasts at the periapical and furcal regions. DM1 exacerbated these characteristics, with a greater impact on bone structure, resulting in a low OPG content and a higher RANKL/OPG ratio, which correlated with prominent osteoclastogenesis. This work demonstrates that the effects of PD and DM1 enhance bone destruction, confirms the importance of the RANKL signaling pathway in bone destruction in DM1 in animal models and suggests the existence of alternative mechanisms potentiating bone degradation in PD.

Viabilidade de sementes de Handroanthus heptaphyllus (Vell.) Mattos armazenadas nos frutos

Pós-graduação em Ciência Florestal - FCA