235 resultados para Microcystis Aeruginosa


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Pós-graduação em Ciências Farmacêuticas - FCFAR

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Pós-graduação em Microbiologia Agropecuária - FCAV

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

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Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Ciências Farmacêuticas - FCFAR

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Biologia Geral e Aplicada - IBB

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In extensive animal husbandry, natural or artificial ponds allow direct access of cattle to water but result in degradation of water quality and in increased health risks. Under such circumstances eutrophication occurs and consequently algal bloom, among them toxigenic cyanobacteria. The present study aimed to report the occurrence of cyanobacteria in the drinking water of cattle and to describe their physical and chemical parameters, as pH, temperature and dissolved oxygen. Nineteen samples of natural ponds or water troughs formed predominantly as result of rainfall from six farms located in the Southeast and Midwest regions of Brazil were analyzed for the presence of cyanobacteria, and pH, temperature and dissolved oxygen was measured. Microcystis and/or Merismopedia were detected in two ponds; one of them was covered with intense flowering. The values of pH, temperature and dissolved oxygen in 19 collections were pH 7.2-9.7, 31-34ºC and 7.8-30mg/l respectively. Also non-pathogenic algae of several genera were detected besides the occurrence or not of cianogenics. Under these conditions, the common practices of drinking water supply for extensively raised cattle and the possibilities of eutrophication and cyanobacterial growth bring potential risks for animal health.

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Pós-graduação em Medicina Veterinária - FMVZ

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Objective: To characterize the microbial etiology of chronic suppurative otitis media comparing the methods of classical bacteriological culture and polymerase chain reaction.Design/Setting/Patients: Bacteriological analysis by classical culture and by molecular polymerase chain reaction of 35 effusion otitis samples from patients with cleft lip and palate attending the Hospital for Rehabilitation of Craniofacial Anomalies of the University of Sao Paulo, Bauru, Brazil.Interventions: Collection of clinical samples of otitis by effusion through the external auditory tube.Main Outcome Measure: Otolaryngologic diagnosis of chronic suppurative otitis media.Results: Positive cultures were obtained from 83% of patients. Among the 31 bacterial lineages the following were isolated. In order of decreasing frequency: Pseudomonas aeruginosa (54.9%), Staphylococcus aureus (25.9%), and Enterococcus faecalis (19.2%). No anaerobes were isolated by culture. The polymerase chain reaction was positive for one or more bacteria investigated in 97.1% of samples. Anaerobe lineages were detected by the polymerase chain reaction method, such as Fusobacterium nucleatum, Bacteroides fragilis, and Peptostreptococcus anaerobius.Conclusions: Patients with cleft lip and palate with chronic suppurative otitis media presented high frequency of bacterial infection in the middle ear. The classical bacteriological culture did not detect strict anaerobes, whose presence was identified by the polymerase chain reaction method.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)