Estudo da diversidade microbiana e níveis de endotoxinas em lesões endo-periodontais

Pós-graduação em Odontologia Restauradora - ICT

Participation of dectin-1 receptor on NETs release against Paracoccidioides brasiliensis: role on extracellular killing

Paracoccidioides brasiliensis is a dimorphic fungus from the Paracoccidioides genus, which is the causative agent of paracoccidioidomycosis, a chronic, subacute or acute mycosis, with visceral and cutaneous involvement. This disease that is acquired through inhalation primarily attacks the lungs but, can spread to other organs. Phagocytic cells as neutrophils play an important role during innate immune response against this fungus, but studies on antifungal activities of these cells are scarce. In addition to their ability to eliminate pathogens by phagocytosis and antimicrobial secretions, neutrophils can trap and kill microorganisms by release of extracellular structures composed by DNA and antimicrobial proteins, called neutrophil extracellular traps (NETs). Here, we provide evidence that P. brasiliensis virulent strain (P. brasiliensis 18) induces NETs release. These structures were well evidenced by scanning electron microscopy, and specific NETs compounds such as histone, elastase and DNA were shown by confocal microscopy. In addition, we have shown that dectin-1 receptor is the main PRR to which fungus binds to induce NETS release. Fungi were ensnared by NETs, denoting the role of these structures in confining the fungus, avoiding dissemination. NETs were also shown to be involved in fungus killing, since fungicidal activity detected before and mainly after neutrophils activation with TNF-α, IFN-γ and GM-CSF was significantly inhibited by cocultures treatment with DNAse.

Diagnóstico dos casos de aprovação nas disciplinas de cálculo diferencial e integral I e II do curso de licenciatura em química - Unesp de Araraquara

Studies show that the disciplines of differential and integral calculus have high failure rates in all schools where they are present and, although this problem has been accentuated in recent years, it is not a local phenomenon. It's observed since 1970s in various Institutions of higher education in Brazil. These studies seek to understand the causes of failures and proposals for teaching methodology that aims to help reduce these rates. However, there is a large contingent of students who are successful at the first time that they perform these disciplines. And these cases, although they have not been studied, can contribute greatly to the understanding the aspects that are related to learning of them. And this understanding can lead to attitudes and learning and teaching methodologies that promote the expansion of this success. The fact that these subjects also have high failure rates in the Degree in Chemistry of this Institute, this study aimed to investigate the factors that were relevant to the cases for approval of the students performed for the first time. For this we constructed a questionnaire containing objective questions and open answers. The objective sought to diagnose the profile of these students with regard to age, gender, basic education (elementary and middle school), study habits, concurrent activities, among others. These questions were analyzed using simple statistics. The open-ended questions asked students to indicate that they attribute the success obtained in the disciplines and to suggest modes of study. These questions were analyzed using thematic categorization, a method of content analysis. This questionnaire was completed, voluntarily and without identification, by students who have passed the first time attended the courses Calculus I and/ or Calculus II and had never started another college prior to this. The results indicate that the most relevant factors in this process were dedication and correct habits...

Nível de conhecimento dos cirurgiões dentistas sobre a qualidade dos prontuários odontológicos para fins de identificação humana

Objective: The aim of this research was to determine the knowledge level of 400 dentists registered in the Regional Dental Council of Cuiabá, through a questionnaire about the importance of records in the process of human identification. Results: We observed that 48.36% of them dispense between 10 and 20 minutes. Only 13.1% of the surveyed dentists did not have the habit of writing down the oral conditions of their patients before starting treatment and 42.62% have only one odontogram filled. From the 122 participants, 11.11% reported that the documentation had no utility to establish the patients identity. To make the situation worse, 33.6% of them said they did not keep the records of patients updated. Conclusion: We conclude that the dentist of Cuiabá has enough knowledge on preparing, maintaining and importance of dental records before the death in the identification process for establishing the patient identity. But, always he did not properly fills the dental records, thus it has been reducing their clinical, administrative and legal value.

Study on Chagas disease occurrence in the municipality of Monte Negro, State of Rondônia, Brazilian Amazon

Introduction: Studies on Chagas disease deal with the perspective of its occurrence in the Amazon region, which is directly correlated to the population growth and the spread of the bug biotope. The state of Rondônia has an immense source of vectors (Triatomine) and reservoirs of Trypanosoma cruzi. Environmental changes brought forth by the deforestation in the region may cause vector behavior changes and bring these vectors to a closer contact with humans, increasing the probability of vector infection. Methods: This study was carried out to check the occurrence of Chagas disease in the municipality of Monte Negro, Rondônia, Brazil, based on a random sampling of the farms and people wherein blood collection from the population and capturing triatomines were done. The blood samples were submitted to serologic tests to detect antibodies of the IgG class against T. cruzi. The triatomines that were collected had their digestive tract checked for the presence of trypanosomatidae with morphology resembling that of the T. cruzi. Results: The population examined was mostly from other states. From the 322 bugs examined on the microscope, 50% showed parasites with morphology compatible with T. cruzi. From the serology of 344 random samples of human blood, 1.2% was found positive, 6% showed inconclusive results, and 92.8% were negative. Conclusions: Monte Negro shows low prevalence of human infection by T. cruzi and none active vector transmission; however, preventive and surveying measures, which are not performed until now, shall be taken due to the abundance of vectors infected by trypanosomatidae.

A compreensão da definição freudiana do conceito de pulsão sexual para a compreensão do conceito de sexualidade humana

This article approaches the definition of sexual drive proposed by Freud in the Three Essays and how the psychoanalytic conception of human sexuality has been theoretically presented from this first exposition: both as a construct of the species and as an individual construct. It seeks to show how the very metapsychological definition of drive (Trieb) – in 1905 and also in Freud’s 1915 Instincts and their Vicissitudes – interlaced biological contributions and the psychoanalytic clinic in the formulation of an original concept of the human sexuality. The paper also discusses how, because of this duplicity, there is sometimes a developmental interpretation of sexuality in the Three Essays. In the first two of the Three essays, Freud tried to expand the possibilities of sexual behavior, analyzing the drives in the diversity of perversions and in children’s sexuality, while in the third essay the focus was on the adult sexual drive from the moment it organizes itself around an object (hence being no longer auto-erotic) and the reproduction function. Certain experts have occasionally questioned whether the 1905 article attributed a biological teleology to the human sexuality by assigning reproduction as the eventual purpose of the sexual drive – that is, a reproductive goal achieved through the sexual intercourse (coitus). Our study seeks to show how the physiological point of view proposed by Freud in his 1915 article on Instincts sheds some light on how the very biological origin of the drives denies this supposed exclusive reproductive purpose of sexuality. The duplicity of Freud’s concept of drive – as expressed in the enigmatic sentence where he states that this is a concept situated on the border between the psychical and the physical – is then discussed taking into account this intercrossing between the biological and the psychical presented in the Three Essays. The points of view proposed by Freud in 1915 for the definition of the concept of drive – the physiological and the biological points of view – are suggested as conceptual tools to the understanding of this twofold character of human sexuality, according to psychoanalysis.

Professores de arquitetura diante da questão do desenvolvimento humano e da inclusão social das pessoas com deficiência

Professors of Architecture on the Subject of Human Development and Social Inclusion of People With Disabilities Historical-Cultural Psychology believes that the people appropriate the culture and develop themselves by social interactions. The architect acts indirectly on human development by producing spaces that allow varying levels of interaction between individuals. To know if the Brazilian Architecture courses are graduating professionals who facilitate the social inclusion of people with disabilities, this research aimed to investigate the concepts of Architecture teachers of a public university about this theme. After the interviews, the reports obtained were submitted to the categorical-content analysis method. Technical concepts predominated over reflective ones, except for the architecture concept, classified as a knowledge area which brings gains to human life.

Síntese e caracterização de pequenos peptídeos lineares do veneno de Tityus serrulatus (Buthidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seletividade de inseticidas a alguns dos inimigos naturais na cultura do algodão

The aim of this work was study the selectivity of insecticides in favor of natural enemies in cotton (Gossypium hirsutum latifolium Hurtch Lr), DeltaOpal cultivar, in the city of Malhada (BA), and to know the associated beneficial fauna. The study was conducted at the agricultural year of 2010/2011. The design was conducted in randomized blocks with six treatments and four replications. The treatments were: (1) Fipronil 200 SC (0.38 L.ha-1); (2) Alphacypermethrin 100 SC (0.30 L.ha-1), (3) Lufenuron 50 EC (0.30 L.ha-1), (4) Imidacloprid 200 SC (0.30 L.ha-1), (5) Methyl parathion 600 EC (1.00 L.ha-1), and (6) control (water). The product was applied 80 days after emergence, and the evaluations were performed one day before application and 1, 7 and 14 days after application (DAA). The samples were taken using the sampling method beating cloth and Moericke traps. Natural enemies were brought to the laboratory for sorting, counting and identification by family. The toxicity of the products ranged according to the group of natural enemies. Imidacloprid is selective to the spiders and insecticides are moderately toxic (Methyl Parathion and Alphacypermethrin: 1 and 14th DAA; Lufenuron: 14th DAA) or toxic (Fipronil and Alphacypermethrin: 7th DAA). Fipronil (1 DAA), Alphacypermethrin (7th DAA) and Methyl  Parathion (14th DAA) are moderately toxic to adult ladybirds. The analyzed insecticides are toxic to the larvae of ladybirds, with more impact until seven days after the application, with the exception of Methyl Parathion classified, as innocuous until this period. The occurrence of 13 families of spiders and 18 families of parasitic Hymenoptera is registered in cotton agroecosystems in the region of Malhada, in the state of Bahia.

O uso da atividade guia (brincar) como catalisadora do desenvolvimento do pensamento e da linguagem

To select pedagogical practices that allow the development of psychic function thinking and psychic function language. It's necessary understanding the whole psyche human, postulated by Vygotsky. The action of playing, the main activity of the children in the childhood education, can enhance the resilience of the psychic functions, once it provides stimulus and may include teacher's intervention during its deployment. For a better intervention, this planning in light of a theory is primordial, as well as the reflection on this practice. This research aimed to find the moment of the activities that took place the development of these functions, language and thought, in children who are in the early years of the childhood education. The survey was conducted through a qualitative interpretation approach, as action research. Data analysis allowed us to observe planned teaching practices in which enabled the development of language and thought and consequently a qualitative leap in the other psychic functions