Síntese e propriedades farmacológicas dos derivados de estilbenos

O resveratrol (3, 5, 4'-triidroxiestilbeno) é um polifenol natural encontrado, principalmente nas cascas das uvas. Este composto possui propriedades anti- inflamatórias, auxiliando assim nas doenças cardiovasculares, como a aterosclerose, devido a sua ação de inibição de vasodilatadores como o NO, possui atividades antioxidantes agindo na inibição da oxidação das LDLs e contribui no tratamento das neoplasias atuando principalmente, em cânceres como o uterino, ovariano e o colorretal. Apesar de inúmeras comprovações de suas propriedades farmacológicas, há poucos estudos sobre sua síntese, as principais rotas existentes são as de Wittig e Heck. Nesta revisão bibliográfica serão abordadas as principais propriedades farmacológicas deste composto, mostrando seus benefícios e sua eficácia à sua saúde humana, além de rotas sintéticas de baixo custo e complexidade de execução para a produção industrial deste estibelno de origem natural.

Impactos de atividades de exploração de petróleo e gás natural sobre a comunicação acústica de Mysticeti (ordem Cetacea): uma revisão

Acoustic communication is essential in mammals and has three main functions: acquisition of information about the environment, intraspecific communication and detection of predators and prey. Studies indicate that the introduction of sounds produced by anthropogenic activities such as military exercises, use of sonar and activities related to the extraction of oil and natural gas can cause interference in cetacean communication. Recently, the discovery of pre-salt tends to increase these activities. After a decade since the launch date of IBAMA`s licensing and before the imminent increase in exploration activities in Brazil, it is essential to conduct studies to monitor closely the impact of this type of activity on the marine ecosystem. Thus, this study aims to identify potential impacts that the process of oil and natural gas exploration and production might have on the communication of baleen whales. Data from literature on bioacoustics and ecology of these animals were linked with technical-scientific data regarding this type of activity. 310 documents related to the topic were analyzed. Among them only 81 documents are of academic origin, and the others mostly action plans and reports from government agencies. 80% of the documents do not have any species as a focus, and in the remaining 20%, 17% were focused on the Greenland Whale (Balaena mysticetus) and 22% on the gray whale (Eschrichtius robustus). The main impacts identified in this study were the increased frequency and amplitude of vocalization, reduction or cessation of more elaborate songs and masking problems

Síntese de novos derivados híbridos do Ácido Acetil Salicílico (AAS)

A aterosclerose é uma doença caracterizada pela perda de elasticidade e espessamento da parede arterial, sendo iniciada pelo acúmulo de lípides, células inflamatórias e elementos fibrosos na parede das artérias. Esses elementos, ao longo do tempo, darão origem a placas ateroscleróticas ou ateromas que podem por ventura se romper, ocasionando no extravasamento de seu conteúdo lipídico central. Tal situação promoverá a adesão e a agregação plaquetária com conseqüente formação de trombo. As doenças cardiovasculares (DCVs) são a principal causa de morte no mundo, sendo por isso bastante estudadas. Entre os recursos terapêuticos disponíveis se podem citar os antiagregantes plaquetários. A terapia com antiagregantes plaquetários apresenta alguns inconvenientes, que vão desde a ineficácia terapêutica em alguns indivíduos até efeitos adversos deletérios, como hemorragia e transtornos gastrointestinais. É devido a esses fatores, acrescidos à elevada prevalência dessas doenças, que se busca a síntese de novos antiagregantes plaquetários, mais efetivos e menos tóxicos para a prevenção e o tratamento das DCVs, sendo o ácido acetilsalicílico (AAS) um dos fármacos de primeira escolha para tratamento. Nesse projeto, usando a hibridação como estratégia de modificação molecular, planejou-se compostos derivados do AAS espaçados pela subunidade N-acilhidrazona contendo ainda uma subunidade doadora de óxido nítrico representado pelos furoxanos e benzofuroxanos. Esses híbridos constituem uma alternativa à “resistência” ao AAS por atuarem em diferentes vias bioquímicas potenciais para inibição da agregação plaquetária

Uso do modelo de simulação 'ABC 6' para avaliação de áreas sujeitas à ocorrência de ondas de cheia e corridas de massa/detritos: estudo de caso na bacia do Ribeirão da Prata (SP)

Pipelines are linear engineering works, designed mostly for transporting oil and its derivatives for long distances, furnishing even the farthermost zones of the country. Due to oil sector needs to ensure for the safety and conservation of its properties, several geotechnical studies are being held at the pipelines field, in order to preserve this important transportation, and also to prevent accidents, which might seriously compromise the environment and the population who lives around it. The OSBRA pipeline, who connects the city of Paulínia to the capital Brasília, is one of these engineering works that deserves to be pointed out. This research, performed at the Ribeirão da Prata Basin, was a pilot study conducted with the main objective of testing the current methodology efficiency, for future applications in the closest watersheds to the OSBRA pipeline. The objective of this research is to analyze flood wave and debris flow processes in a non-fictional watershed, by comparing two different kinds of methods: the first one based on simulation models (software ‘ABC 6’), and the other one by flood wave and debris flow susceptibility mapping. The results from the hydrological modeling were both hydrographs and ietographs that estimated values of outputs and infiltration. To construct the susceptibility maps were necessary three other maps: ground use and occupation maps, divided according to the different protection degrees that were offered to the ground; maps of dam locations in the area and physiographic compartimentation maps, divided according to the local geology. To complete the methodology, the results were collected from both methods for comparison. The obtained product for this methodology was series of data whose different susceptibility degrees to flood wave and debris flow could define the safest route for a pipeline crossing in this watershed...

Avaliação da toxicidade sobre Ceriodaphnia dubia das águas dos rios Atibaia, Jaguari e efluente tratado de refinaria de petróleo

Ensuring availability of quality water for human consumption causes becomes an increasing number of studies for the analysis of effluent before and after treatment, so that its release into receiving bodies do not cause significant changes in the river water and on the biological communities related to them. The biomarkers of toxicity have long been used to verify the potential toxic effluent and its correlation with the treatment efficiency of them. In this context, the cladoceran Ceriodaphnia dubia stands out because it is a benthic aquatic organism highly sensitive to environmental changes. In this study, to evaluate the interference of the release of treated wastewater of oil refinery in Rio Atibaia, we sought to determine the toxicity on population dynamics of Ceriodaphnia dubia microcrustacean in water samples upstream and downstream from the launch site, compared with samples from Rio Jaguarí at the point of capture by the company itself and treated effluent. We have studied the number of offspring produced in 10 replicates, each starting with a test individual of up to 24 hours for each sample and correlated the results with physical-chemical and microbiological tests performed by a laboratory technician. For most tests, the results indicate that the treated effluent gives sub-lethal toxicity to the microcrustacean, as delay the onset of the reproductive cycle of the same

Avaliação do método de classificação baseada no objeto em imagens de alta resolução espacial aplicado para o monitoramento de faixa de dutos: estudo de caso no entorno da refinaria de Capuava em Mauá - SP

The metropolitan region of São Paulo is the most populous of the country, this happens because of its great importance in the national economy and the job opportunities that are offered to the population. These factors result in intense population growth and urban expansion, reaching some non-habitable places of the metropolis, as areas of pipelines, which are very important for the transportation of natural gas, oil and its derivatives. Before the population growth of the region, these sites were unoccupied, do not presenting problems for the population. However, with the disorderly occupation is generated great anthropogenic pressure on the pipeline stitches, causing risks to people who are around them. Therefore it is extremely important to monitor the strip of pipelines through products and techniques of remote sensing and geoprocessing, enabling, through high spatial resolution images, identification of objects or phenomena that occur on Earth's surface that can alter the functioning and safety of pipelines. Therefore, this study aims to monitor a stretch of the area of the pipeline mesh GASPAL/OSVAT and Capuava Refinery (RECAP), located on the outskirts of the metropolitan area of São Paulo in the city of Mauá, who suffer great human pressure, proving thus the techniques of remote sensing and geographic information system (GIS) as effective tools for monitoring phenomena occurred in urban areas of great complexity. The monitoring was done by object-based classification applied in orbital images Ikonos II and RapidEye, of high spatial resolution and, image processing, detection of objects, segmentation, classification and editing were developed through the eCognition and ArcGis softwares. To determine the statistical accuracy of the mapping of the land cover of the stretch of pipeline in Maua, the results were analyzed by error matrix... (Complete abstract click electronic access below)

Planejamento, síntese e avaliação da capacidade de doação de óxido nítrico de novos derivados antiinflamatóios

A formação do trombo arterial, composto predominantemente por plaquetas, se dá sob condições de estresse de cisalhamento elevado, nos locais de lesão aterosclerótica vascular e fluxo sanguíneo perturbado e tem como consequência diminuição ou obstrução do calibre dos vasos dos diferentes órgãos podendo levar a eventos isquêmicos. As doenças cardiovasculares são consideradas as principais causas de morte no mundo. Nesse contexto, estima-se que a aterosclerose/aterotrombose seja a causa primária de doença arterial coronariana e acidente vascular cerebral representado mais de 50% das causas de mortes em países ocidentais . É sabido que a aterosclerose, primeiro evento que predispõe à aterotrombose, é considerada um processo inflamatório crônico, e que a utilização de antiinflamatórios não esteroidais (AINEs) COX-1, melhor estabilizam a estrutura da placa ateromatosa impedindo o rompimento e a consequente formação do trombo. Neste contexto, no presente trabalho foram obtidos de novos candidatos a fármacos com propriedades doadoras de NO e antiagregantes plaquetárias, através da estratégia de hibridação molecular dos AINEs contendo uma subunidade doadora de óxido nítrico, representada pelos furoxanos, e espaçada por uma subunidade N-acil hidrazônica. Dessa forma, a inibição da síntese de tromboxanos associada à doação de óxido nítrico permitiu atuação nas plaquetas através de um mecanismo múltiplo de ação com fins de diminuição da agregação plaquetária. Os produtos intermediários e finais apresentaram bons rendimentos e foram caracterizados por técnicas analíticas as quais predisseram que as estruturas propostas foram obtidas. Todos os compostos se como doadores de óxido nítrico. Os compostos 1a e 3a apresentaram atividade analgésica superior a dipirona, utilizada como controle. No ensaio de agregação plaquetária ―in vitro‖... (Resumo completo, clicar acesso eletrônico abaixo)

Helibacter pylori: preaparação de Lapdesf-IUNI e ensaio de inibição de urease (IC50) de derivados de tiouréia e guanidínicos

O presente trabalho de conclusão de curso visou à otimização e preparação da síntese de Lapdesf-IUNI e avaliação do potencial de inibição de urease de três compostos previamente sintetizados, direcionados especificamente para o tratamento de infecções por Helicobacter pylori, bactéria mundialmente reconhecida como uma das principais causadoras de infecções no mundo, responsável pelos casos de infecções gástricas, como gastrites crônicas, úlceras (gástricas e duodenais) e carcinomas. Lapdesf-IUNI é um composto derivado de metronidazol, com atividade potencialmente inibidora de urease, enzima presente neste microrganismo e essencial a sua sobrevivência no ambiente gástrico. O produto foi obtido, porém com baixo rendimento, por isso sua metodologia ainda necessita ser aperfeiçoada, para que, em seguida este composto possa ser testado. Outros três compostos, um derivado de tiouréia e dois derivados guanidínicos, com potencial atividade inibidora de urease, T-6, R-17 e R-21, sintetizados pela equipe do Prof. Eduardo Perez, foram avaliados com relação a sua atividade inibidora frente à enzima urease e IC50, apresentando bons resultados, que permitem a continuidade dos estudos, comparando inclusive com fármacos utilizados nos protocolos de tratamento padrões para o tratamento das infecções causadas por H. pylori

Estudo da tecnologia e processos de extração em poços de petróleo

The analysis of the extraction process in oil wells is presented exploring the challenges to the effectiveness of the procedure is shown step by step in the process. There is an analysis of the major tools and equipment needed in the drilling process, promoting the understanding of the challenges in the pre-salt layers, compared to conventional processes. The work is reinforced through studies of the influence of the corrosive compared not corrosive environment in drill strings

Eficiência das resinas XAD4 e C18 na extração de compostos mutagênicos presentes em efluente de refinaria de petróleo para a realização do Teste de Ames

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Análise da expressão gênica de pequenos RNAs derivados de tRNAs (tRFs) em plantas

Small non coding RNAs emerged as important characters in several biology aspects. Among then, the most studied are microRNAs (miRNAs) and short interfering RNAs (siRNAs), that regulate their target gene post-transcriptionally in plants, animals and RNAi pathway intermediates, respectively. Both of classes have similar biogenesis being processed by Dicer enzymes and subsequent association with Argonaute enzymes. In plants, miRNAs and siRNAs have important functions in development, genome integrity and biotic and abiotic stress responses. The advances in high-throughtput sequencing and in silico analisys provide the uncover of new small non coding RNAs classes, many of them with unknown functions and biogenesis. tRNA derived small RNAs (tRFs) are a small non coding RNA class, that have as precursor a tRNA molecule. These were uncovers in the last decade in many organisms and, recently, in plants. Recent works detected tRFs from different sizes, with different source portions of the mature tRNA molecule (5’ end; 3’ end, anti-codon loop) and some from the tRNA precursor (pre-tRNA), suggesting that may be a novel class of small RNA and not random degradation products. Works in humans showed that some tRFs are processed by the Dicer enzymes, have association with the Argonaute enzymes and cell differentiation, tumor appearance and gene silencing related functions. Works in Arabidopsis and pumpkin (Cucurbita maxima) showed, respectively, that the tRFs have nutritional stress response possible functions and long distance signaling function between source and drain tissues, and may affect the translation. The tRFs biogenesis in plants are, until now an unknown, absence information about it in the literature and its possible biological functions are few studied yet, making then interesting target for studies among the small non coding RNAs in plants

Análise dos royalties do petróleo sob a perspectiva jurídica

This research aims to discuss the division of royalties from a legal perspective and its substitute role towards the non-taxation at source, in other words the taxation of destiny at the Brazilian petroleum industry. The analysis aims to deal with the historical evolution in relation to the aspect of royalties’ payment and its deviation of compensatory character in order to supply the ICMS function. There are some specific study objects in this research: 1. how the transformation of royalties’ payment led to financial compensation; 2. to whom the transference of mineral rent belongs – to the UNIÃO or to the fortunate regions which the oil is extracted; 3. would a tax reform be enough to minimize the competition of States and Cities towards the royalties? The research aims to clarify the real expectation that States have on the royalties’ payment. There is no compensation for damage to the environment as it is stated by the initial characteristic of the Constitution, and, by the contrast, from a compensatory character, there is a lacking of taxation from the sector mentioned before, this fact damages the producing states checked out by this research

Medidas de adequação ambiental para indústria de leite e derivados, como requisito para gestão ambiental

The society continues demonstrating its need regarding the products and services, even though it becomes increasingly value the protection of the environment. In this context, environmental management is defined as a management method that emphasizes to continuously improve results and promote sustainable development. Moreover it has as a tool the laws which provide the benchmarks and mechanisms for companies to adapt their activities in such a way that does not alter the quality of the environment. In this sense, in order to propose improvements to a dairy aiming their environmental suitability, a present study was prepared by following the production activities of the enterprise, the raising of standards and applicable legal requirements and environmental assessment for the preparation of an array of aspects and impacts with the task of identifying the most significant according to MOREIRA (2006). The results indicated that some of the impacts of high relevance are related to the generation of wastewater and solid waste, which do not have treatment and / or proper disposal and indeed require intervention for their suitability. Through, these results were drawn up proposals for actions conformation. In addition, they were proposed meeting the requirement for health and safety at work, in accordance with the standard regulations. This study has the function, therefore, propose to the company in question, an environmental management plan that ensures the protection of the environment and health of employees and consequently the improvement of production processes and products

Foraminíferos de manguezais do rio Curimataú (RN): estudos sinecológicos com aplicação no setor de petróleo e gás

O sistema estuarino do Rio Curimataú, uma planície lamosa amplamente coberta por vegetação de mangue e relativamente pouco impactada, foi estudado quanto à distribuição espacial da microfauna de foraminíferos e suas condicionantes. Vinte e cinco gêneros e quarenta espécies foram identificadas a partir dos sedimentos superficiais, considerando-se os indivíduos vivos e mortos presentes em 10 pontos ao longo do estuário, dos quais se extraíram ao menos 100 espécimes por amostra. O sistema pôde ser compartimentado em quatro sub-ambientes halínicos (hiperhalino, mesohalino, polihalino e euhalino), correspondentes, respectivamente, a quatro ecofácies de foraminíferos designadas de Ammotium spp., Miliammina fusca, Arenoparrella mexicana e Ammonia spp. Cada um destes segmentos ambientais foi caracterizado segundo os padrões de riqueza, diversidade e equitatividade das espécies. Os resultados deste estudo poderão servir para comparações a serem feitas com outros estuários tropicais modernos (impactados antropicamente ou não) e antigos. Tem aplicação, portanto, no setor de petróleo e gás, sendo de interesse às atividades de gestão ambiental de áreas litorâneas e de investigação da história geológica das bacias sedimentares

Avaliação da tenacidade em um aço microligado, grau API, para aplicação em linhas dutoviárias de petróleo e gás

In order to study the mechanical properties of micro alloyed steel API 5L X70, a material used to manufacture pipes for pipeline transportation lines for use in oil and gas, a study was made of toughness, tensile strength, impact strength, hardness and microstructure steel. To perform these various tests were made where they can acquire the characteristics of the material. Were performed at the Faculty of Engineering in Guaratinguetá in the Department of Materials and Technology and the tensile tests, Charpy impact test, metallography and hardness testing of material API 5L X70, all tests were done with the help of technical laboratories. With these data can be an analysis of the material about his tenacity, his toughness and fragility, its hardness, its yield strength and its maximum voltage. After being asked the analyzes discussed the results showed that the micro alloyed steel API 5L X70 steel is a very tenacious, it absorbs impact energy of 300 Joules though without a break for the full body of evidence showing its tenacity