Efeito de vernizes fluoretados suplementados com trimetafosfato de sódio na desmineralização e remineralização do esmalte bovino: estudos in vitro e in situ

Pós-graduação em Ciência Odontólogica - FOA

Desenvolvimento de sistemas líquido cristalinos lamelares contendo diferentes concentrações de Ac-Wahx-kttks para o tratamento do envelhecimento cutâneo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência da diabetes mellitus na resposta tecidual e na capacidade de mineralização dos cimentos Sealapex, MTA Angelus e MTA Fillapex

Pós-graduação em Ciência Odontólogica - FOA

Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer

The Cell-dyn 3500 is a multiparameter flow cytometer, which may analyze samples from several species performing several simultaneous analyses. It is able to perform white blood cells, red blood cells and platelet counts, besides differential leukocyte counts, packed cell volume and hemoglobin determination. Cell-Dyn 3500 performs total leukocyte count both optically and by impedance. The equipment may choose one or other method, based on the reliability of the results. Erythrocyte and platelet counts are determined by impedance. Leukocyte differentiation is based on an optical principle, using separation in multiangular polarized light. The objective of this study was to compare the results of complete blood count of Zebu Nellore heifers from Cell-dyn 3500, with those obtained from a semi-automated cell counter (Celm CC 510) and the manual technique. Blood samples were collected from the jugular vein in 5 mL EDTA vacuum tubes from 58 Nellore heifers, at 24 months of age. Samples were processed in parallel in the three different techniques. Results were analyzed using paired t test, Pearson's correlation and the Bland-Altmann method. There was a strong correlation for all parameters analyzed by Cell-Dyn 3500, manual method and semi-automated cell counter, except for basophils and monocytes counts. These results confirm that this analyzer is reliable for blood samples analysis of zebu cattle.

Avaliação da resposta tecidual e capacidade de mineralização de cimentos que contém compostos biocerâmicos, resinosos e com hidróxido de cálcio

Pós-graduação em Ciência Odontólogica - FOA

Regulação microambiental de células epiteliais límbicas corneais de coelho expandidas sobre membrana amniótica humana desepitelizada: ênfase para a organização supramolecular dos maiores biopolímeros extracelulares

Pós-graduação em Cirurgia Veterinária - FCAV

Influence of diabetes mellitus on tissue response to MTA and its ability to stimulate mineralization

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Multifunction hexagonal liquid-crystal containing modified surface TiO2 nanoparticles and terpinen-4-ol for controlled release

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Dendrimer nanocarriers for transport modulation across models of the pulmonary epithelium

The purpose of this study was to determine the effect of PEGylation on the interaction of poly(amidoamine) (PAMAM) dendrimer nanocarriers (DNCs) with in vitro and in vivo models of the pulmonary epithelium. Generation-3 PAMAM dendrimers with varying surface densities of PEG 1000 Da were synthesized and characterized. The results revealed that the apical to basolateral transport of DNCs across polarized Calu-3 monolayers increases with an increase in PEG surface density. DNC having the greatest number of PEG groups (n = 25) on their surface traversed at a rate 10-fold greater than its non-PEGylated counterpart, in spite of their larger size. This behavior was attributed to a significant reduction in charge density upon PEGylation. We also observed that PEGylation can be used to modulate cellular internalization. The total uptake of PEG-free DNC into polarized Calu-3 monolayers was 12% (w/w) vs 2% (w/w) for that with 25 PEGs. Polarization is also shown to be of great relevance in studying this in vitro model of the lung epithelium. The rate of absorption of DNCs administered to mice lungs increased dramatically when conjugated with 25 PEG groups, thus supporting the in vitro results. The exposure obtained for the DNC with 25PEG was determined to be very high, with peak plasma concentrations reaching 5 mu gmL(-1) within 3 h. The combined in vitro and in vivo results shown here demonstrate that PEGylation can be potentially used to modulate the internalization and transport of DNCs across the pulmonary epithelium. Modified dendrimers thereby may serve as a valuable platform that can be tailored to target the lung tissue for treating local diseases, or the circulation, using the lung as pathway to the bloodstream, for systemic delivery.

Relationship between composition and organizational levels of nanostructured systems formed by Oleth 10 and PPG-5-Ceteth-20 for potential drug delivery

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of time interval between bleaching and bonding on tag formation

The objective of this study was to assess penetration of adhesive material in enamel bleached with 35% hydrogen peroxide using optical polarized light microscopy. Extracted human teeth were randomly assigned to 5 groups, each representing a specific time interval between bleaching and the application of an adhesive material. They were designated as: (TC) the control group-restorations in unbleached teeth; (T0) comprising restorations carried out immediately after bleaching; (T7) comprising restorations 7 days after bleaching; (T14) comprising restorations 14 days after bleaching; and (T21) comprising restorations 21 days after bleaching. Length of resin tags was measured with an Axiophot photomicroscope at a x 400 magnification, and the results subjected to an ANOVA for a comparison between groups, with a p value of < 0.05. Differences between the groups were verified using a Tukey test at a confidence level of 5%. The specimens in the control group (TC) and experimental groups T7, T14 and T21 showed better penetration of adhesive material into enamel in comparison with experimental group T0. This suggests that a gap of at least 7 days should be left between bleaching enamel with 35% hydrogen peroxide and placing adhesive bonding agents and undertaking resin composite restoration work.

Influence of corrosion on lipopolysaccharide affinity for two different titanium materials

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Demineralization and hydrogen peroxide penetration in teeth with incipient lesions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis

Women often develop vaginal infections that are caused primarily by organisms of the genus Candida. The current treatments of vaginal candidiasis usually involve azole-based antifungals, though fungal resistance to these compounds has become prevalent. Therefore, much attention has been given to molecules with antifungal properties from natural sources, such as curcumin (CUR). However, CUR has poor solubility in aqueous solvents and poor oral bioavailability. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for vaginal administration. A low-viscosity LCPM (F) consisting of 40% wt/wt polyoxpropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, 50% wt/wt oleic acid, and 10% wt/wt chitosan dispersion at 0.5% with the addition of 16% poloxamer 407 was developed to take advantage of the lyotropic phase behavior of this formulation. Notably, F could transform into liquid crystal systems when diluted with artificial vaginal mucus at ratios of 1:3 and 1:1 (wt/wt), resulting in the formation of F30 and F100, respectively. Polarized light microscopy and rheological studies revealed that F behaved like an isotropic formulation, whereas F30 and F100 behaved like an anisotropic liquid crystalline system (LCS). Moreover, F30 and F100 presented higher mucoadhesion to porcine vaginal mucosa than F. The analysis of the in vitro activity against Candida albicans revealed that CUR-loaded F was more potent against standard and clinical strains compared with a CUR solution. Therefore, the vaginal administration of CUR-loaded LCPMs represents a promising platform for the treatment of vaginal candidiasis.

Caracterização histoquímica e imuno-histoquímica das alterações fibróticas nas endometroses das éguas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)