Efeito do genótipo e do teor protéico da dieta na resistência de cordeiros à hemoncose experimental

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Expressão gênica no parênquima mamário de novilhas leiteiras

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effect of nutritional density and season on the performance of young rabbit does before the first mating

The objective was to evaluate the effect of nutritional density and season on the performance of young rabbit does before the first mating. Forty-eight females from the Botucatu Genetic Group were used in each season (warm or cool), starting at the age of 70 days, housed in groups of four per cage up to 119 days, and reared individually from 120 to 140 days of age. The high-density diet was formulated to contain, on a 90% DM basis, 18.4% CP, 16.5% ADF and 2,500 kcal DE kg-1; whereas the low-density diet was formulated to contain 14.7% CP, 24% ADF and 2,000 kcal DE kg-1. The experiment was conducted according to a 2x2 factorial design (two diets x two seasons) with repeated measures (weeks). The high-density diet promoted lower feed intake, higher final weight, and higher daily weight gain, adjusted for constant intake. But these facts alone do not warrant using a high-density diet for growing rabbit does, because it could have a negative impact during the reproductive phase. In the warm season, there was a reduction in feed intake and an improvement in feed efficiency. Along time, however, the mean body weight of does was similar in the two seasons.

Influência da fuligem da queima de palha de cana-de-açucar na presença de HPA em hortaliças de consumo humano

Pós-graduação em Química - IQ

Effects of alcohol intake in periodontitis progression in female rats: a histometric study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Simbióticos e ionóforo em dietas para bovinos mestiços Angus: desempenho, características de carcaça e qualidade de carne

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Consumo, digestibilidade e balanço de nitrogênio de cordeiros alimentados com alto teor de amido ou fibra solúvel em detergente neutro associados ao óleo de girassol

The aim of this study was to evaluate the effect of different carbohydrate sources associated with sunflower oil on intake, digestibility of nutrients and nitrogen balance in diets of lambs. Twenty lambs were used, assigned in a completely randomized design with a 2 x 2 factorial arrangement of treatments with two sources of neutral detergent soluble carbohydrate, starch and neutral detergent soluble fiber, with and without the inclusion of 4.2% sunflower oil. The dry matter intake was not affected by carbohydrate sources and the inclusion of oil. Diets with a high percentage of starch provided higher digestibility of dry matter, starch and ether extract. The different sources of carbohydrates had no interference on digestibility of organic matter, crude protein, neutral detergent soluble fiber, nonfiber carbohydrates, neutral detergent fiber, hemicellulose and acid detergent fiber. The addition of oil in the diets increased the digestibility of ether extract. The combination of 4.2% oil in the diet high in soluble neutral detergent fiber had no influence on the nutrient digestibility, otherwise, the addition of oil at high starch diet caused a significant reduction in the digestibility of organic matter, neutral detergent fiber, hemicellulose and nonfiber carbohydrates. The quantities of nitrogen intake, absorbed and retained, did not differ as sources of carbohydrates and oil inclusion in the diet. The association of 4.2 % sunflower oil to the different sources of carbohydrates in the diet does not affect consumption and nitrogen metabolism in lambs. The addition of 4.2 % oil in the diet with high starch (28% of DM diet) promotes reduction in digestibility of organic matter, of fibrous and nonfibrous carbohydrate in the diet.

Avaliação dos efeitos do cogumelo do sol (Agaricus blazei) sobre o colesterol plasmático em coelhos com hipercolesterolemia induzida

Prophylactic or therapeutic treatments administered with medicinal plants and natural products are used in popular medicine of diverse people since prehistoric times to the present days. Species with medicinal properties are increasingly studied in an attempt to understand their possible effects on organisms and their functioning. This study aimed to analyze the effect of the mushroom Agaricus blazei (aqueous extract) in rabbits subjected to experimental hypercholesterolemia. The animals were divided into two groups (control and treated with the mushroom) whose experimental protocol was divided into three phases: Phase 1, the animals were fed a normal diet to evaluate the physiological level of cholesterol; phase 2, the animals were fed a supplemented diet to induce hypercholesterolemia and in phase 3, the animals of control group continued to take high-cholesterol diet and the animals of treated group high-cholesterol diet including treatment with the mushroom. Weekly, after fasting of 14 hours, blood samples were collected from the animals and its plasma was stored for later measurement of plasmatic cholesterol. In the first phase, the cholesterol level was, on average, 31,30  7,34 mgdL-1. In the second phase, there was a significant increase (p<0,05) in cholesterol level of both groups. During the last phase of the experiment, the mushrooms didn’t cause reduction in plasmatic cholesterol of treated rabbits, however, prevent disease progression, maintaining the cholesterol level established at the beginning of treatment, whereas, in the control group, total serum cholesterol increased significantly at this stage

Atividade hipolidêmica e protetora da bixina no fígado de ratos tratados com dieta hipercalórica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Obesogenic environment by excess of dietary fats in different phases of development reduces spermatic efficiency of wistar rats at adulthood: correlations with metabolic status

This study compares the impact of obesogenic environment (OE) in six different periods of development on sperm parameters and the testicular structure of adult rats and their correlations with sex steroid and metabolic scenario. Wistar rats were exposed to OE during gestation (O1), during gestation/lactation (O2), from weaning to adulthood (O3), from lactation to adulthood (O4), from gestation to sexual maturity (O5), and after sexual maturation (O6). OE was induced by a 20% fat diet, and control groups were fed a balanced diet (4% fat). Serum leptin levels and adiposity index indicate that all groups were obese, except for O1. Three progressive levels of impaired metabolic status were observed: O1 presented insulin resistance, O2 were insulin resistant and obese, and groups O3, O4, and O5 were insulin resistant, obese, and diabetic. These three levels of metabolic damage were proportional to the increase of leptin and decreased circulating testosterone. The impairment in the daily sperm production (DSP) paralleled these three levels of metabolic and hormonal damage being marginal in O1, increasing in O2, and being higher in groups O3, O4, O5, and O6. None of the OE periods affected the sperm transit time in the epididymis, and the lower sperm reserves were caused mainly by impaired DSP. In conclusion, OE during sexual maturation markedly reduces the DSP at adulthood in the rat. A severe reduction in the DSP also occurs in OE exposure during gestation/lactation but not in gestation, indicating that breast-feeding is a critical period for spermatogenic impairment under obesogenic conditions.

Effect of diets with varying starch content on muscle glycogen concentrations during training and replenishment after highintensity exercise

Pós-graduação em Zootecnia - FCAV

Legumin from chickpea: hypolipidemic effect in the liver of hypercholesterolemic rats

Purpose – This paper aims to determine the effects of 11S globulin isolated from Chickpea (Cicer arietinum L.) on lipid metabolism in animals subjected to a hypercholesterolemic and hyperlipidemic diet and compared to the drug simvastatin. Design/methodology/approach – Thirty-six male Wistar rats, kept in individual cages and under appropriate conditions, were separated into groups that were fed a normal diet (STD) containing casein as protein source and according to AIN-93G; a high-cholesterol diet (HC), normal diet plus 1 per cent cholesterol and 0.5 per cent cholic acid and 20 per cent coconut oil; HC diet plus the isolated 11S globulin (300 mg/kg/day); and HC diet plus the simvastatin (50 mg/kg/day), both dissolved in saline and administered by gavage for 28 days. After this time, the animals were killed. Findings – The results indicated that the addition of 1 per cent cholesterol and 0.5 per cent cholic acid induced hypercholesterolemia in the animals without interfering with their weight gain. Analyses of total cholesterol (TC), HDL-cholesterol (HDL-C) and triglycerides (TG) in the plasma, and TC and TG in the liver were made. The results show that the protein isolated from chickpea, and given as a single daily dose, did not affect the levels of plasma TC and its fractions, although decreasing the TG levels. Unlike the simvastatin, the chickpea protein significantly reduced TC and TG in the liver relative to HC group. Originality/value – A single daily dose of 11S globulin from chickpea contributed as only as additional 2.8 per cent of dietary protein intake. These findings demonstrate that 11S chickpea protein acts as a functional agent in the lipid metabolism in addition to its nutritional properties.

Acidose ruminal subaguda em ovinos Santa Inês: estudo clínico, laboratorial e avaliação da laminite por termografia infravermelha e radiologia digital

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de um embutido cárneo fermentado, com teores reduzidos de gordura e sais de cura, através da utilização de culturas probióticas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The adverse effect of a high energy dense diet on cardiac tissue

Purpose: To determine whether a high energy dense diet intake increases oxidative stress and alters antioxidant enzymes in cardiac tissue. Design: A randomized, controlled study. Ninety-day-old female rats were randomly divided into two groups: one fed with a low energy dense diet (LE; 3.0 kcal g-1) and one with a high energy dense diet (HE; 4.5 kcal g-1). Materials and Methods: After 8 weeks of treatment, the animals were fasted overnight and sacrificed by decapitation. The serum was used for glucose, triacylglycerol, cholesterol, low-density lipoprotein (LDL)-cholesterol and high-density lipoprotein (HDL)-cholesterol determinations. The glycogen, lipoperoxide, lipid hydroperoxide, superoxide dismutase, glutathione peroxidase, lactate dehydrogenase, citrate synthase, total and non-protein sulphhydryl groups were determined in cardiac tissue. Results: HE decreased the myocardial glycogen content and increased the lactate dehydrogenase/citrate synthase ratio, indicating an increased glycolytic pathway and a shift from myocardial aerobic metabolism. HE-treated female rats showed increased lipoperoxide and hydroperoxide levels in cardiac tissue. Although no alterations were observed in the total sulphhydryl group and superoxide dismutase activities, glutathione peroxidase and the non-protein sulphhydryl group were significantly decreased in HE-treated animals. Conclusions: Although no alterations were observed in energy intake, HE induced an increased intake of fat and carbohydrate and an increased rate of weight gain. HE intake induced alterations in markers of oxidative stress in cardiac tissue. Hydrogen peroxide is an important toxic intermediate in the development of cardiac oxidative stress by HE. The specific nutrient content, such as fat and carbohydrate, rather than caloric intake, appears to be the main process inducing oxidative stress in HE-treated female rats.