Avaliação histológica do processo de reparação do tecido ósseo do rato frente à aplicação de um adesivo à base de cianoacrilato

This research objective was to verify the tissues reactions obtained, in surgical experimental bone defect, after the etil cyanoacrylate chemical adhesive application in rats parietal bones. 1t was investigated the acceptance or no acceptance of the tissues surround the bone defect in which was placed the etil cyanoacrylate and the control bone defect. The specimens were submitted to light microscopic analysis. 24 adults rats were used (Rattus norvergicus, albinus, Wistar) with the average weight to 300 gr. and were divided in two groups: treated and control. After the bone defects were realized, etil cyanoacrylate was placed over one of the defects at the parietal bone (treated group). On the other defect nothing was placed and it was used like control group. After 24 hours, 3,5,7,30 and sixty days after surgery, for animals of each group were sacrificed. The bone samples were removed and fixed in 10% formalin during 72 hours and analyzed using light microscopy. The results showed that the acute inflammatory responses was more observed at lhe treated group than the control group. The chemical adhesive was observed until 60 days follow the surgery and the tissues around it present normal appearance. The chemical adhesive, etil cyanaocrylate, did not impede at the bone repair process

Efeito de três sistemas de osteotomia - ultrassom cirúrgico, laser Er, Cr: YSGG e sistema rotatório - sobre o processo de osseointegração e reparo de defeitos ósseos em tíbia de ratos: estudo histomorfométrico, imuno-histoquímico e biomecânico

Pós-graduação em Odontologia - FOAR

Influência do estímulo verbal no tempo do teste de escada

Introduction: The stair-climbing test (SCT) is considered a submaximal test of simple implementation and easy access, which assesses the individual’s functional capacity. Although widely used in surgical patients, there is no standardization regarding the height of the stair and verbal stimulus. Objectives: It was determine if verbal stimulus changes the stair-climbing time (SCt) in individuals over 50 years-old. We compared oxygenation, blood pressure, pulse, respiratory rate and Borg scale between SCT performed with and without stimulation. Methods: We evaluated individuals with ages greater than 50 years-old that performed two STC (with and without verbal stimulation), in the stair with 44 steps, achieving 7.04 m in height and recording the time to climb the stair. The systolic blood pressure (SBP) and the diastolic blood pressure (DBP), respiratory rate (RR), pulse, oxygen saturation (SpO2 ) and Borg scale were evaluated before and after the tests. The time in the SCT with and without stimulation were compared using the Student test-t and the other variables were compared using the ANOVA test (p<0.05). Results: The average age was 59.75±6,40 years old in the 21 evaluated individuals. The time in the SCT without stimulus was significantly higher than the one with stimulus. The SBP, pulse, RR and Borg scale were significantly increased when compared to the SCT with and without stimulus. Oxygenation and DBP didn´t have significant differences at any time of the study. Conclusions: The time in the SCT was lower when performed with verbal stimulus. The SBP, pulse, RR and Borg scale changed significantly after the SCT with and without stimulus, remarking that this change was greater in the SCT with stimulus.

Análise do processo de reparo de defeitos ósseos críticos em calvária de ratos preenchidos com osso autógeno ou Bone Ceramic: um estudo histométrico e imunoistoquímico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Simulação numérica através do método dos elementos finitos e comparação experimental para obtenção de tensões e deformações específicas em fêmur com prótese total de quadril

Pós-graduação em Engenharia Mecânica - FEG

Aplicações da ilustração científica em ciências biológicas

A Ilustração Científica é um trabalho que consiste na representação fiel de um material biológico determinado, respeitando-se todas as medidas, proporções e contraste de cores, mesmo que em preto e branco. Por isso, a capacidade de observação é uma qualidade indispensável para que se tenha um bom resultado. Podem ser desenhos de materiais vivos, extintos, arqueológicos e até processos cirúrgicos e devem ser claro em significado, não poluído e que não provoque nenhum tipo de incompreensão ou dúvidas. Ela é altamente utilizada no meio acadêmico tendo um vasto campo de aplicação, variando desde trabalhos mais simples e esquemáticos aos mais complexos com alto nível de acabamento e detalhamento. Independentemente à técnica, as ilustrações científicas ainda são uma ferramenta de extrema importância e de grande utilização, sendo muito solicitadas por pesquisadores, que desejam enriquecer seus trabalhos, torná-los mais explicativos, mais claros, e mais didáticos. Por mais que se tenha a possibilidade das fotografias modernas, um desenho empregando técnicas tradicionais como acabamento é totalmente indispensável, já que a foto, por mais sofisticada e cheia de recursos que possua a máquina, não consegue transmitir todos os detalhes e estruturas que se deseja em uma mesma fotografia. O artista tem a liberdade de montar uma prancha com aquilo que o pesquisador precisa, usando sua percepção visual e destreza para reproduzir com exatidão o material em questão. Recursos digitais, ao contrário do que muitos acreditam, não tiraram o espaço das técnicas tradicionais, mas veio como mais uma opção de ferramenta de trabalho que, aliás, para usá-la é necessário saber desenhar igualmente àquele que não se utiliza desse recurso. O tempo passou, as sociedades mudaram e as representações...(Resumo completo, clicar acesso eletrônico abaixo)

Etiopatogenia e tratamento do deslocamento de abomaso em bovinos leiteiros de alta produção

The dairy business is in constant development, in order to achieve better results and higher profits to the producer, and this search for improvement has led to the selection of a herd more productive. But this has caused some problems heightened, among them the displacement of the abomasum, a disease that affects much of the high producing dairy herd. The main reasons are apparently related to the feeding management, with the abrupt changes that the animal suffers in the post partum period, with diseases that would cause abomasal atony favoring its displacement, among others. When affected, the animals lose their appetite and in milk production, and may even, in severe cases, death to come. Treatments described in literature are varied and there are conservative and chirurgical methods. The choice of a particular technique depends on the financial condition of the producer, the economic value of the cow and from personal experience and technique of the veterinarian. Understanding the clinical implications of displaced abomasum is necessary not only for its animal identification in the field and its appropriate treatment but also for finding the errors and inadequacies in the management system of production that trigger this disease

Considerações clínicas em implantes imediatos

The biological principles of osseointegration caused the rehabilitation treatment with osseointegrated implant become a safe, well alternative accepted by the dental community for the high success rate, allowing the preparation of functional and aesthetic prostheses in edentulous and partial patients. We passed the initial phase of functional vision for an aesthetic approach, too, depending on the demands of the patient and the quest for excellence by professionals. Over these last years, implant treatment has undergone many changes in surgical and prosthetic protocols. The less invasive surgical techniques and the development of restorative materials, especially ceramics allow the prosthetic rehabilitation of high functional quality and aesthetics. The installation of implants in sockets immediately after extraction of teeth involved by fracture, periodontal disease, endodontic lesions, is part of the arsenal of techniques for rehabilitating a number of advantages that we get to the outcome of multidisciplinary treatment. For this, we must consider several criteria in its planning as a fundamental unit, adjacent teeth, multiple losses, system, form and number of implants, type of retention of the prosthesis components, the patient expectation of the outcome perspective, gingival biotype, standard of ridge resorption, the smile line, etc. occlusal analysis. With the three-dimensional placement of the implant can immediately join the biological processes of repair of the socket, implant osseointegration, speeding treatment time. Much has been made by companies in the surface treatment of endosseous implants to be osseointegration shortened and restorative procedures started earlier, bringing benefits to the patient and professional. Among the main advantages we can emphasize the preservation of the structures adjacent to the teeth replaced, minor resorption of bone tissue involved... (Complete abstract click electronic access below)

Checklist de cirurgias da OMS: análise da segurança e comunicação da equipe cirúrgica de um hospital escola

WHO has launched the “Safe Surgery Saves Lives” program with the purpose to reduce damage to surgical patients and define safety standards specifically in order to reduce such patients’ morbi-mortality by providing surgery teams and hospital managers with orientation about the standards of safe surgery and a uniform service evaluation instrument for national and international surveillance. Hence, this study aimed at applying the WHO safe-surgery checklist to the surgical specialties of a university hospital and evaluating the team’s opinion about the influence of its application on the safety of surgical process and on the team’s interpersonal communication. It was a descriptive, analytical, qualitative field study conducted in the surgery facilities of a university hospital in a public establishment in São Paulo state. The checklist was applied to eight surgical specialties, resulting in a total number of 30 surgeries. Its application was conducted by the researcher in three phases: Sign in, Time out, Sign Out. Next, one member of the surgery team was invited to voluntarily participate in the study by signing an informed consent form and answering guiding questions. Thirty members of the surgery team participated in the study. Bardin’s Content Analysis Method was used to organize and analyze the data. As regards the safety provided by the checklist, the following thematic categories emerged: “It reduces risk and possible complications”; “It standardizes conducts and reviews safety steps”; “It allows for better understanding of the process”; and “It provides safety to the team as a whole”. The category “It is not included in the institution’s routine” emerged from the subjects’ statements when they understood that, in this form, the checklist does not provide safety to surgical procedures. As regards communication, two thematic categories emerged: ...(Complete abstract click electronic access below)

Avaliação do Efeito Fotodinâmico sobre o Crescimento in vitro de Pythium insidiosum

Photodynamic Therapy (PDT) is a therapeutic method which employs a photosensitizer and light to cause cellular death. The chemical compounds have low or none toxicity for hosts cells. Under the incidence of light, in an appropriated wavelength, these chemical compounds produce reactive oxygen which affects the biomolecules of the target-cells. The specific illumination of the affected area increases the selectivity of the therapy, since the photodynamic process occurs only in the irradiated area. Pythiosis, for instance, is a life-threatening emerging disease caused by a fungus-like organism called Pythium insidiosum. The disease occurs in man and other animals, being mostly observed in horses. Human pythiosis may present as ophthalmic, cutaneous-subcutaneous and systemic forms of lesions. Due to the fact that P. insidiosum is not a true fungus, it is refractory to most antifungal drugs and the treatment of the disease is difficult. Extensive surgery procedures, such as limb amputation, are the treatment of choice, however relapses may occur frequently. Although not totally effective, the use of immunotherapy associated to surgery have shown some results. Considering that pythiosis is an emerging disease few explored in its etiological and therapeutic aspects, which are limited and few effective, it is of great importance to encourage the development of researches for new strategies of treatment. In this sense, it was evaluated the effect of PDT on in vitro growth of the pathogen employing two chemical compounds as photosensitizer, porphyrin and chlorine, at different concentrations in combination with several energetic dosages. Porphyrin showed inhibition of growth at 25mg/mL with 100J/cm2 of energetic dosage and chlorine showed similar results employing low concentrations (0,7, 1,0 and 1,3mg/mL) with 70J/cm2 of energetic dosage... (Complete abstract click electronic access below)

Produção de biofilme por bastonetes gram negativos isolados de água de hemodiálise

No Brasil, 90% de pacientes com insuficiência renal crônica ou aguda dependem dos procedimentos de hemodiálise para remover produtos de degradação metabólica, excesso de água e de sais minerais do organismo, a fim de restaurar o equilíbrio ácido-base e eletrolítico. Entretanto, o processo de osmose reversa, que visa remover todos os íons da água, também remove o cloro, que exerce efeito bacteriostático sob diversas bactérias autóctones, que passam a se multiplicar na água. Entre os principais grupos dessas bactérias, estão os bastonetes Gram negativos não fermentadores (BGN) e os mais frequentemente associados a bacteremias, em pacientes de hemodiálise são Pseudomonas aeruginosa, Stenotrophomonas maltophilia e Burkholderia cepacia. Essas bactérias podem produzir biofilmes, o que torna sua eliminação do encanamento quase impossível. Assim, o objetivo do trabalho foi a análise de água e do dialisato, da Unidade de Hemodiálise de um Hospital Universitário, na pesquisa dos três BGNs citados. Também foram utilizadas 42 cepas previamente isoladas do mesmo local. Entre as 67 amostras de água, foram isoladas 8 cepas de Pseudomonas aeruginosa e as 50 cepas foram submetidas à pesquisa do gene rpoS, um dos responsáveis pela produção de biofilme. Esse gene foi observado em 20 (40%) cepas, sendo 16 (80%) P. aeruginosa, seguida por 3 (15%) cepas de S. maltophilia e 1 (5%) de B. cepacia. Essas cepas foram submetidas à produção de biofilme a 35ºC (temperatura ótima de crescimento) e a 20ºC (temperatura média da água no encanamento da Unidade de Hemodiálise), em aço inoxidável (material de muitos instrumentos cirúrgicos), PVC (matéria prima da tubulação) e plástico de poliestireno (capacidade de produção de biofilmes em diferentes materiais). Em poliestireno, somente 1 (5%) cepa de P. aeruginosa produziu biofilme a 35ºC e a 20ºC, 2 (10%) cepas foram... (Resumo completo, clicar acesso eletrônico abaixo)

Cirurgias seguras salvam vidas: aplicação e avaliação do checklist sugerido pela OMS em cirurgias infantis de um hospital escola

The World Health Organization (WHO) has created a program “Safe Surgery Saves Lives”, focusing on reduce and minimize life threatening or cause serious risk to health for patients submitted to a surgery. This primary goal for this presented study is to apply the checklist from the WHO´s program for children´s surgery in a public university hospital, located in the countryside of São Paulo State, Brazil. Having the responses from professionals surgery´s team, there is an opportunity for evaluation of the features and easiest parts, which are filled in a quickly and in a easy way; focus, supporting and assistance; issues, such as lack of time for filling in, and viability of application of this protocol considering the improvement of the cross functions relationship, and quality of services, providing better assistance and support to the patient

Associações neuroleptoanalgésicas em equinos

The equine anesthesia is proven to be a high risk procedure, for both patient and anesthetist, compared to other animal species. In these conditions, a good anesthetic protocol that can deliver sedation, making access and manipulation easier, analgesia fair enough to realize cirurgical procedures, and minimal physiologic changes would be ideal. Since there aren’t any drugs suporting all these qualities, the Neuroleptoanalgesic Associations show themselves as a valuable technique to provide anesthetic quality and safety, possibly diminishing our drug of choice’s doses, therefore offering less fisiological changes and easier monetary access in some cases. This paper objective was to list all these associations found in literature, evaluating their advantages and practical applications

Processo de reparo ósseo diante de três tipos de superfície de titânio: análise histomorfométrica e por microscopia eletrônica de varredura associada ao EDS: estudo experimental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O ensino da implantodontia no curso de graduação de odontologia: o relato de experiência de uma instituição e o perfil dos pacientes atendidos na clínica de implantodontia das Faculdades Adamantinenses Integradas - FAI

Pós-graduação em Odontologia Preventiva e Social - FOA