Influences of the pH on the adsorption properties of an antimicrobial peptide on titanium surfaces

The adsorption behavior of the Tet-124 antimicrobial peptide and the Tet-124 peptide modified at the C- and N-terminus with the sequence glycine-3,4-dihydroxyphenylalanine-glycine (G-DOPA-G) on titanium surfaces was studied using quartz crystal micro balance with dissipation (QCM-D). At a low pH level (4.75) Tet-124 and Tet-124-G-DOPA-G form rigid layers. This is attributed to the electrostatic interactions of the positively charged lysine and arginine residues in the peptide sequence with the negatively charged titanium oxide layer. At an elevated pH level (6.9) Tet-124 shows a lower mass adsorption at the surface than Tet-124-G-DOPA-G. This is attributed to the interaction of the catechol due to the formation of complexes with the titanium oxide and titanium surface layer. The C terminal and N terminal modification with the sequence G-DOPA-G shows similar adsorption rate and mass adsorption coverage at saturation; however it is presented a more loosely layers on the G-DOPA-G-TeT-124. Fibroblast adhesion and the biocompatibility test of both the surfaces following modification with Tet-124-G-DOPA-G and the titanium alloy control showed similar results. In addition, no changes in the adhesion of E. coli bacteria due to the modification of the surface were detected.

Diurnal evolution of the hourly diffuse solar radiation incident on tilted surfaces in Southeast of Brazil

Evaluation has been made on the monthly and annual average diurnal evolution of the hourly diffuse radiation as well as its radiometric fractions on surfaces inclined at 12.85, 22.85 and 32.85° to face North, in climate conditions of Botucatu, São Paulo, Brazil (22.85° S and 48.43° W). Measurements were made between 04/1998 to 08/2001 for 22.85°; 09/2001 to 02/2003 for 12.85° and 01/2004 to 12/2007 for 32.85°, with concomitant measures in the horizontal. For all surfaces the diffuse radiation was obtained from different method. Assessment has been performed as well on the radiometric fractions obtained from the ratio of diffuse radiation and global radiation (KDH and KDβ) and between radiation and diffuse radiation at the top of the atmosphere (KʹDH and KʹDβ) for the horizontal and tilted surfaces in hourly partition. The diffuse radiation levels were dependent on variations in precipitation and cloudiness. There was an increase in the differences between the diffuse radiation and the radiometric fractions with the increment of the angle, and in horizontally, which affected higher levels of diffuse radiation in spring and summer. The values of KDH and KDβ present in an inverse behavior were compared to diffuse radiation and theydecreased in the southern passage due to the increase of the direct component in the total of incident radiation.