Expressão de receptores Toll-like - 2 e Toll-like - 4 em membranas corioamnióticas de gestações complicadas por corioamnionite

Corioamnionite é definida como inflamação das membranas corioamnióticas, sendo que tal inflamação resulta geralmente de infecção bacteriana do líquido amniótico, das membranas fetais e da placenta. O sistema imune inato constitui a primeira linha de defesa do hospedeiro contra patógenos e nesse sentido os receptores Toll-like (TLR) são importantes reguladores dessa resposta inespecífica. Entretanto, a expressão desses receptores nas membranas corioamnióticas de gestações complicadas por corioamnionite não está bem estabelecida. Investigar a expressão de receptores Toll-like -2 e -4 em membranas corioamnióticas de gestações complicadas por corioamnionite. Foram incluídas no estudo 48 membranas corioamnióticas, coletadas no Serviço de Obstetrícia do Hospital das Clínicas da Faculdade de Medicina de Botucatu, UNESP, no período de janeiro a novembro de 2008, de gestações pré-termo e de termo, incluindo gestantes com rotura prematura de membranas pré-termo (RPM-PT), trabalho de parto pré-termo (TPP) além de gestações de termo (GT). Fragmentos das membranas corioamnióticas foram encaminhados à análise histopatológica para confirmação de corioamnionite histológica. Outros fragmentos de 1cm2 das membranas foram acondicionados em RNA later® e foram submetidos à extração de RNA total. Após a extração do RNA, as amostras com concentração entre 0,02 e 0,2μg/ μL de RNA foram submetidas à obtenção de cDNA para posterior utilização na quantificação da expressão de TRL-2 e TLR-4 pela técnica da PCR em tempo real empregando-se o sistema TaqMan® Gene Expression Assays. Dentre as 24 membranas corioamnióticas com presença de corioamnionite, 41,7% foram obtidas de... (Resumo completo, clicar acesso eletrônico abaixo)

Deslocamento de abomaso em vacas leiteiras

The genetic selection and the nutritional management to improve milk production make the dairy cattle more susceptible to the development of diseases, such as the abomasal displacement. It is the most frequently detected abomasal problem and it is the main cause of abdominal surgeries in dairy cattle. It is a multifactorial disease that occurs mainly in dairy cattle of high production during the puerperium. The abomasal displacement can occur to the right (DAD) or to the left (DAE), being the former more frequent than the latter. It is related to feeding management and occurs in animals that also have other diseases such as hypocalcaemia, ketosis and retained placenta. The disease causes economical losses in dairy cattle because of the costs with treatment, reduction of production, increase of the interval between the parturition, loss of body weight, early discard of the matrix and mortality. The most usual clinical signs are apathy, dehydration, low to serious ruminal timpanismo (gas accumulation in the abomasum) with reduction or lack of motility, liquid splash sound during the ballottement of the right flank, metallic sound to percussion, presence of a structure similar to distended viscera in the thorax or in the paralombar cavity on the side corresponding to the displacement, and liquefied, dark, scarce and fetid feces. The treatment is surgical, and the most used technique is the omentopexy on the left flank. The hidroelectrolytic correction must be performed and the concomitant diseases must be treated. The prophylaxis consists of adequate nutrition and pre-parturition management, besides reduction of stress and other diseases of the puerperium

IL-10 e TNF-α no sangue materno e nas placentas de gestações complicadas por diabete ou hiperglicemia gestacional leve - correlação com controle glicêmico e resultados perinatais

Objective - At evaluating the IL-10 production in maternal blood and placenta and correlate them with perinatal outcomes in pregnancies complicated by hyperglycemia, or with risk to developing it, differentiated by the glycemic mean (GM < or ≥ 100mg/dL). Method- 186 pregnant women were distributed into groups GM < 100 mg/dL and GM ≥ 100 mg/dL. We evaluated the GM, HbA1c levels, maternal and placental IL-10 and TNF-α and the correlation between placental cytokines and perinatal outcomes. Results - In maternal blood, the lower concentrations of IL-10 (1.01 ± 0.87 vs. 3.08 ± 5.57 pg / mL, p = 0.0019) were observed in GM ≥ 100 mg / dL group. Placental IL-10 was directly correlated with hemoglobin levels (r = 0.63, p = 0.02) and insulin (r = 0.78, p = 0.01) from umbilical cord and with Apgar scores 1 (r = 0.53, p = 0.0095) and Apgar 5 (r = 0.69, p = 0.0003). Conclusion - GM ≥ 100mg/dL was associated with decreased of maternal IL-10. Placental IL-10 was similar in both groups and correlated directly with hemoglobin and insulin and with Apgar scores of 1st. and 5th. minutes

Sanidade Uterina Pós Parto: Aspectos Imunológicos e Terapêuticos

Function of the uterus is often compromised in cattle by bacterial contamination of the uterine lumen after parturition. Pathogenic bacteria often persist, causing uterine disease, a major cause of infertility in cattle. Knowledge of the immunological aspects of the uterus involved in maintaining reproductive healthiness is fundamental to the study of uterine infections that affect the uterus postpartum. Polymorphonuclear leukocytes have an important role because they are the first line of defense against colonization of bacteria in utero (Hammon and Goff, 2006). The establishment of uterine infection depends in parts of endocrine environment, particularly progesterone, which suppresses the immune system (Lewis, 2003). In the puerperium may occur uterine disorders, such as retained placenta, puerperal metritis, clinical and subclinical endometritis and pyometra, this review was proposed a study of the immunology involved in uterine health and a better understanding of uterine disorders, using the model of Sheldon et al. al, (2006) for classification of diseases, and a study of best treatment options and discussion about its functionality, because a lot of controversy among authors about choosing a treatment and another and between treated and untreated

Proliferação celular em gestações naturais e de conceptos bovinos transgênicos e clonados, que expressam a proteína fluorescente verde (GFP)

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Changes in the TNF-alpha/IL-10 ratio in hyperglycemia-associated pregnancies

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Different intensities of exercise (swimming) applied to pregnant rats: maternal and fetal outcomes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of moderate intensity exercise on the reproductive performance of diabetic rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hyperglycemia effects on epigenetic regulation of pax3 during the early embryonic development

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Increased il-1 β production in placental villous subjected to hyperglycemic conditions

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Intrauterine growth restricted rats exercised at pregnancy: maternal-fetal repercussions

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Long term repercussions in the offspring of diabetic rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Maternal diabetes: cytokine profile in maternal and cord blood

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)