Imunoglobulinas e C3 no granuloma paracoccidióidico

Utilizou-se o modelo experimental de paracoccidioidomicose, em camundongos, induzida pela inoculação endovenosa de suspensão de formas cerebriformes do P. brasiliensis (cepa Bt2; 1x10(6) formas viáveis/animal), para avaliar, após 2, 4, 8, 16 e 20 semanas: 1. A presença de imunoglobulinas e C3 nos granulomas pulmonares, por imunofluorescência direta; 2. A resposta imune humoral (imunodifusão) e celular (teste do coxim plantar), e 3. A histopatologia das lesões. Os camundongos apresentaram resposta imunocelular positiva desde a 2a. semana, com depressão transitória na 16a. semana, e anticorpos desde a 4a. semana, com pico na 16a. semana. Os granulomas pulmonares foram epitelióides, com numerosos fungos e microabscesses; a extensão das lesões foi progressiva até a 16a. semana, com regressão discreta na 20a. semana. Desde a 2a. semana, houve deposição de IgG e C3 na parede dos fungos no interior dos granulomas e a presença de células IgG positivas no halo linfomononuclear periférico; estes achados foram máximos entre a 4a. e 16a. semanas. Não se detectou depósito de IgG e C3 no interstício dos granulomas. IgG e C3 parecem exercer papel precoce e importante na defesa do hospedeiro contra o P. brasiliensis, contribuindo possivelmente para a destruição dos fungos e bloqueando a difusão de antígenos para fora dos granulomas.

Correlations between preweaning traits in Duroc swine

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Diferenciação hepatocítica de células-tronco mesenquimais da medula óssea humana

Some recent articles have reported that mesenchymal stem cells (MSCs) can be induced to express hepatocyte markers by transplanting them into animal models of liver damage, or by in vitro culture with growth factors and cytokines. In this study, the aim is to evaluate the behavior of MSCs subjected to induction of hepatocyte differentiation. The MSCs were isolated from the bone marrow of 4 normal donors, characterized and subjected to both in vitro and in vivo induction of hepatocyte differentiation. The in vitro induced cells showed morphological changes, acquiring hepatocyte-like features. However, the immunophenotype of these cells was not modified. The induced cells exhibited no increase in albumin, cytokeratin 18 or cytokeratin 19 transcripts, when analyzed by real-time RT-PCR. The expression of albumin, cytokeratin 18 and alpha fetoprotein was also unchanged, according to immunofluorescence tests. In vivo, the MSC demonstrated a potential to migrate to damaged liver tissue in immunodeficient mice. Taken together, the results suggest that bone marrow MSCs are incapable of in vitro differentiation into hepatocytes by the approach used here, but are capable of homing to damaged hepatic tissue in vivo, suggesting a role for them in the repair of the liver. This contribution to tissue repair could be associated with a paracrine effect exerted by these cells.

Estimativa de alguns parâmetros de transporte de contaminantes para solos Brasileiros

This paper introduces an indirect estimate for the coefficients of distribution, hydrodynamic dispersion and retardation for contaminants commonly encountered in sanitary landfills and their liners, such as Cu2+ and K+; this estimate is based on the relationship between concentration and certain physical characteristics of typical Brazilian soils. The results of previous studies investigating the migration of contaminants were used to develop mathematical expressions from multiple non-linear regressions. Using minimal squares regression, this transport was linked to various combinations of contaminant concentration and both structural and textural characteristics of the porous medium. Various combinations of characteristics and concentrations were investigated, with a mathematical expression obtained for each. The relationship between percentage of clay and the contaminant content proved to be the most closely correlated with actual transport parameters, with coefficients close to one.

Predição da potência aeróbia (VO 2máx) de crianças e adolescentes em teste incremental na esteira rolante

The maximal oxygen uptake (VO2max) is the maximal quantity of energy that can be produced by the aerobic metabolism in certain time unity. It can be determined direct or indirectly by predictive equations. The objective of this study was to make a specific predictive equation to determine the VO 2max from boys aged 10-16 years-old. Forty-two boys underwent a treadmill running ergospirometric test, with the initial velocity set at 9 km/h, until voluntary exhaustion. By the multiple linear regression was possible to develop the following equation for the indirect determination of the VO 2max: VO2max (ml/min) = -1574.06 + (141.38 x Vpeak) + (48.34 * Body mass), with standard error of estimate = 191.5 ml/min (4.10 ml/kg/min) and coefficient of determination = 0.934. We suggest that this formula is appropriate to predict VO2max for this population.

Parâmetros genéticos entre caracteres quantitativos no girassol como critério de seleção para produtividade de aquênios

Two experiments were conducted in southern Brazil in no-tillage system, in order to estimate genetic parameters and the direct and indirect effects of components for achene yield as a selection criterion in sunflower. We analyzed eight sunflower hybrids at two locations, in a randomized complete block design with four replicates, determined through quantitative descriptors: insertion height of the head, and head stem diameter, weight of 1000 achenes, number of achenes per head, mass by achene head and yield achene. Estimates of genetic parameters were based on combined analysis, decomposing interactions in genetic and environmental components. Considering the coefficient of genetic variation, indirect effects of components and heritability, there are higher possibilities for responses to selection in sunflower achenes by descriptors mass and mass of achenes per head, with its indirect association interrelated pathways for the increase in the achenes of yield.

Suscetibilidade magnética do solo e estimação da capacidade de suporte à aplicação de vinhaça

The objective of this work was to evaluate the magnetic susceptibility efficiency for estimating the support capacity of areas for vinasse application. Two hundred forty-one soil samples were collected from a 380-ha area, on which soil chemical properties, clay content, and magnetic susceptibility were determined. Vinasse requirement was calculated for each sample. Data were subjected to descriptive statistical analysis, and regression models were developed between magnetic susceptibility and the other evaluated attributes. The analysis of data spatial dependence was performed using geostatistics. Kriging maps and cross variograms were built in order to investigate the spatial correlation between soil magnetic susceptibility and studied attributes. Based on the map of vinasse requirement, on the soil classes, and on the kriging map, calculations were done for average vinasse dose and average soil support capacity, weighted by the area. Magnetic susceptibility has significant linear spatial correlation with recommended vinasse doses and soil support capacity for the application of this effluent, and it can be used as a pedotransfer function for indirect quantification of soil support capacity.

Correlação e análise de trilha de produtividade de grãos e seus componentes e caracteres de planta em cártamo (Carthamus tinctorius L.) e mamona (Ricinus communis L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Uso do clorofilômetro portátil na determinação da adubação nitrogenada de cobertura em cultivares de feijoeiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Atributos da validação do método analítico para quantificação da biotina empregando a técnica potenciométrica

Pós-graduação em Alimentos e Nutrição - FCFAR

Efeito da suplementação de taurina na oxidação de substratos energéticos e no desempenho de atletas nadadores

Pós-graduação em Alimentos e Nutrição - FCFAR

A construção de sentidos na avaliação de múltipla escolha do SARESP

Pós-graduação em Estudos Linguísticos - IBILCE

Indivisibilidades entre natureza, homem e expressão artística: a reflexão estética de Merleau-Ponty

Pós-graduação em Artes - IA

Efeito dos exercícios aeróbio contínuo e com pesos combinados sobre a sinalização insulínica e transportador de glicose em musculatura esquelética de ratos obesos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise do gasto energético e composição corporal de adolescentes obesos sedentários e de adolescentes obesos submetidos a treinamento concorrente

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)