Anticorpos contra Leptospira spp. e Brucella spp. em equiídeos da região Sul do Brasil abatidos em matadouro-frigorífico

Pós-graduação em Medicina Veterinária - FCAV

Foto seleção de mielomas e hibridomas murinos na produção de anticorpos monoclonais

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Desenvolvimento de um eletrodo modificado com monocamadas auto-organizadas e sua utilização como biossesor

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Síntese, caracterização e estudo da auto-associação em solução aquosa de derivados anfifílicos zwiteriônicos de quitosana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Anticorpos naturais e infecção: anticorpos naturais na doença de Chagas e na paracoccidioidomicose

Using ELISA technique, natural antibodies against self and non self antigens were determined in 80 patients chronically intected by T. cruzi and 40 individuals suffering from a deep mycosis frequentely found in Latin Amarica (Paracoccidioidomycosis - PCM). Two forms of PCM were investigated: adult forms and juvenil type of disease. Eighty percent (80%) of the former group had significantly elevated anti-laminin antibody levels (M=4.7,SD±1.8) compared with healthy controls and different specificities of antibody were associated with anti-laminin in pathological sera. A notable binding to cytoskeletal proteins was observed, specially with band 3 and their peptides derivates, such as 62 kDa peptide. By means of Protein A chromatography we were able to show that natural anti-Gal antibodies may be bound by their Fab region to other immunoglobulins and/or to Protein A by alternative sites of binding. The finding of lgG anti-Gal antibodies in circulating immune complexes isolated from chagasic sera supported the first alternative. However, it is possible that some of lgG anti-Gal antibodies, belong to VH111 subgroup of immunoglobulins, that bind directly to Protein A. Among the 40 sera from PCM examined, the majority was considered as not exhibiting a signilicantly higher binding than normal sera to antigens tested. However thirty percent (30%) of the chronic patients had an increased levels of natural antibodies at least for one specificity such as actyn, myosin and Gala1,3Gal epitopes. ln juvenil type of PCM the mean value found for actyn was also increased 2,42 (range 1,0 to 5,3). Utilizing the polyethylene glicol precipitation the presence of circulating immune complexes was investigated in PCM sera. Specific antibodies for soluble antigens from P. brasiliensis and natural antibodies against myoglobin, myosin and Gala1,3 Gal epitopes were characterized

Auto-organização e autonomia em Hegel: uma abordagem a partir das linhas fundamentais da filosofia do direito

The German philosopher G.W.F. Hegel (1770-1831) had as one of his thesis in the doctorate the affirmation that philosophy can only be properly taken as a system. He intends with that to point out the identity between thinking and being. This is, for Hegel, the task of philosophy, i. e., to bring together all that was set apart. This position can be recognized in all his works and it is tried here to exemplify it in The Philosophy of Right and in special in the section dedicated to the abstract right. This one is the first moment of the text in question that begins with the statement of the dignity of the person confirmed in the property. The following moments make evident that the formal ones are also results. It becomes clear in this way that the all the moments create together an ongoing organization, disorganization and reorganization. This process reveals the relation as a constitutive element of thinking and being exemplified in the abstract right. Each moment overcomes the preceding one and comes out as a much more complex one. In the case of the abstract right what appears is the realization of a free subject in all that he does as the making of himself.

Desenvolvimento das habilidade de auto-cuidado em crianças com atraso neuropsicomotor

Introduction: The neuropsicomotor development could be negatively affected by several factors occurred in the pre, peri and pos natal period. The early stimulation is implemented by a set of dynamic activities which aim the child development according to the child’s phase. Objective: The purpose of this study was to analyze the functional skills acquisition in the self care area in children with development delay. Participated of this study 9 caregiver of children aged of 0 to 3 years with delay neuropsicomotor. Method: For the data collect was used the self-care area of the Pediatric Evaluation of Disability Inventory (PEDI) in the version adapted for Brazil. The data collect was performed in two different stages, with an interval of 6 months between the collects. The raw score were transformed in continuous score by the Rasch statistics, then was applied the Shapiro-Wilk and t-student test. Results: The participants obtained a signifi cant higher score in the second stage than in the fi rst (p<0,01). Conclusion: The results of the present study suggests that the early stimulation could be an important intervention in the process of self-care skills acquisition in children with neuropsicomotor delay.

Influência da ingestão do triptofano dietético sobre a preferência na escolha do macho pela fêmea, auto-balanceamento da dieta e desempenho zootécnico em tilápia-do-Nilo e Pirapitinga

Pós-graduação em Aquicultura - FCAV

Desenvolvimento de imunossensor impedimétrico para detecção do corante disperso Red 1

Pós-graduação em Química - IQ

Produção de anticorpos monoclonais murinos dirigidos contra células-tronco mesenquimais de coelho

Mesenchymal stem cells (MSCs) are a heterogeneous population of cells that proliferate in vitro as plastic-adherent cells, have fibroblast-like morphology and can differentiate into bone, cartilage and fat cells. Therapeutic potential of MSCs have been studied in experimental models, such as rabbit, in Laboratory of Cell Engineering of Botucatu. However, no specific markers have been reported for expanded rabbit MSCs, which hampers the isolation of pure MSC populations by immunophenotypic characterization. Thus, the objective of this study was to produce monoclonal antibodies (mAbs) to rabbit MSCs. MSCs derived from rabbit bone marrow (BM) were isolated, cultured, expanded ex vivo, and immunized into three BALB/c mices, and spleen cells subsequently harvested were used to generate hibridoma cell lines secreting antibodies against MSCs. Hybridoma cells were screened by flow cytometry and antibody-producing cells were subjected to subsequent rounds of retests. MSC1-160 obtained the best positivity for IgG expression and was cloned by limiting dilutions and micromanipulation. Ascitic fluid from ten best clones was purified by affinity chromatography in Protein A-sepharose CL-4B column and purification control was performed by electrophoresis in agarose gels. The purified IgG were tested against rabbit MSCs, obtaining high positivity by flow Cytometry. In conclusion, we developed 10 mAbs, MSC1-160 A20, A30, A41, A47, A55, A60, A63, A69, A81, and A82, that recognize rabbit MSC cell surface antigens showing potential for immunophenotypic characterization of rabbit MSC cell lines

Avaliação da auto-suficiência energética em reatores anaeróbios aplicados ao tratamento de resíduos da indústria de produção do etanol

Ethanol, the main automotive biofuel, has its production based on the fermentation of sugars found in biological materials and on the distillation of the alcoholic media formed during the fermentative process. Stillage is the main residue from ethanol production, containing a high organic loading in addition to acidic and corrosive characteristics. Considering the available technologies to treat stillage, we highlight anaerobic digestion, which allows the reduction of the impacts associated to pollutants loading of this effluent and the generation of energy from the methane gas produced in the process. Based on the high treatment efficiency usually associated to the anaerobic process, this work aimed to assess whether anaerobic systems applied to the treatment of stillage are energetically self-sufficient. First we evaluated the energy recovery capacity in an anaerobic reactor applied to the treatment of stillage resulting from corn-to-ethanol processing. The results indicated the great influence that a correct selection of electrical equipment and their respective operating periods have on the net energy balance of the anaerobic treatment. The high energy consumption of the heater would not allow the system to achieve a positive net energy balance – the maximum energy recovery would reach only 0.68% of the consumption. However, the replacement of the mixture equipment would result in energy gains ranging from 8.5 to 967.9% of the consumption. In this work we also assessed the efficiency of methane yields for a few studies and the correlation between some parameters of the anaerobic process. With respect to the methane yield, we noted that mesophilic systems tend to be more advantageous than the thermophilic ones (efficiency of 76.45 ± 22.51% vs. 69.40 ± 30.36%). Considering the study... (Complete abstract click electronic access below)

Avaliação da auto-suficiência energética de reatores anaeróbios no tratamento de esgoto sanitário e água cinza

The anaerobic treatment of sewage is widely employed in Brazil and it is an appreciated way for the treatment of effluents, helping to reduce the environmental impact in rivers. The methane gas obtained from the process can be applied to improve the energetic efficiency of the system, reducing the amount of waste and the cost of the treatment process. This work presents the net energy balance of anaerobic reactors applied to the treatment of sewage. The analysis was performed considering full-scale and laboratory-scale treatment systems. In laboratory scale, the results from three kinds of systems were compared regarding the biological treatment of greywater. Two of them (UASB7 and UASB12) were anaerobic and the other one was a combined anaerobic-aerobic system (UASB7/SBR6). Greywater methanization (compared to theoretical maximum) was calculated considering 100% removal (g BOD/day), the literature percentage removal and the anionic surfactant presence in the effluentt. For each of these three cases, the efficiencies were, respectively, 16.9%, 43.6% and 51.3% in UASB7 reactor, 25.6%, 50.3% and 59.2% in UASB12 reactor and 30.6%, 61.2% and 71.9% in UASB7/SBR6 reactor. The energetic potential was found to be 4.66x10-4, 7.77x10-4 and 5.12x10-4 kWh/L for the UASB7, UASB12 and UASB7/SBR6 reactors, respectively. The pumping system, the aeration (in the anaerobic-aerobic system) and the temperature controlled heating system were considered to calculate the energetic consumption. However, the third one was not employed since tropical regions like Brazil do not need heating systems and also because of its high energetic consumption. The calculated net energy balance in the reactors was negative in the case of greywater, respectively -0.16, -0.28 and -0.18 kWh/L for the reactors UASB7, UASB12 and UASB7/SRB6. In full scale (ETE Jardim das Flores - Rio Claro, SP), the average energy... (Complete abstract click electronic access below)

Direcionamento de peptídeos tolerogênicos da proteína de choque térmico 60 para células dendríticas utilizando anticorpos anti-DEC-205

A indução de tolerância imunológica em situações altamente desejadas como no transplante e em doenças autoimunes permanece um grande desafio para pesquisa científica de tradução. Nesse contexto, o estudo das proteínas do choque térmico (HSPs) e seus peptídeos vêm trazendo informações relevantes sobre o controle da reposta imune. Dados da literatura mostram que alguns de seus peptídeos apresentam propriedades imunorreguladoras, como os peptídeos N3 e N7 da HSP60. Além disso, tem se mostrado que a apresentação de antígenos em contextos específicos por células dendríticas (DCs) pode favorecer o estabelecimento da tolerância imunológica. Dessa forma, o direcionamento dos peptídeos tolerogênicos da HSP60, N3 e N7, in vivo, para DCs, com o intuito de essas células apresentarem esses peptídeos em um contexto imunorregulador, pode induzir um estado de tolerância. Assim, nesse trabalho, tivemos como objetivo a produção de anticorpos (Acs) contra o receptor DEC-205 de DCs conjugados com os peptídeos N3 e N7 da HSP60. Esses Acs, ao se ligarem ao DEC-205 nas DCs, podem ser fagocitados, processados e por fim apresentados a células T em um contexto imunorregulador. Para tal, foram realizadas transfecções de células HEK-293T e CHO com plasmídeos codificando as cadeias leve e pesada dos respectivos Acs a fim de se obter essas proteínas recombinantes. Podemos observar que as células HEK apresentaram uma produção mais eficiente dos Acs quando comparadas com as células CHO (120 vs 30 ng/ml, respectivamente), apesar disso a produção dos Acs ficou abaixo do esperado impossibilitando a realização ensaios in vivo. Além disso, realizamos um ensaio de ligação do Ac anti-DEC-N7 a superfície de DCs e observamos que os Acs produzidos apresentam capacidade de se ligarem a superfície dessas células. Concluímos que os anticorpos recombinantes anti-DEC-205 são capazes... (Resumo completo, clicar acesso eletrônico abaixo)

Auto-ecologia de Geonoma gamiova Barb. Rodr. (Arecaceae) em mata ombrófila densa montana (Parque Estadual Carlos Botelho, SP)

Geonoma é um dos mais complexos gêneros de palmeiras neotropicais e possui aproximadamente 80 espécies distribuídas nos Neotrópicos. Espécies desse gênero apresentam pequeno a médio porte e são típicas de sub-bosque de florestas em áreas com grande pluviosidade. Este trabalho teve como objetivo analisar a auto-ecologia de Geonoma gamiova em duas escalas espaciais, relacionando sua estrutura populacional entre hábitats (fundo de vale x encosta) e microsítios (local de ocorrência clonal). O trabalho foi realizado no núcleo São Miguel Arcanjo do Parque Estadual Carlos Botelho que possui uma área de 37.794 ha e uma amplitude altitudinal de 50 a 975 metros. A área apresenta relevo de montanhas com vales profundos e morros paralelos e a vegetação presente no Parque é a Floresta Ombrófila Densa. Foram distribuídas 30 parcelas de 5 x 20m em três trilhas no Parque, onde foram quantificados os indivíduos de Geonoma gamiova em três estágios de desenvolvimento e foi caracterizado o hábitat. Foram marcados 42 indivíduos de Geonoma gamiova para avaliar o crescimento vegetativo e sua ecologia reprodutiva, avaliando-se também a estrutura dos microsítios em que esses indivíduos se encontravam. Os indivíduos apresentaram uma distribuição de tamanho populacional em forma de J invertido. Geonoma gamiova foi a segunda palmeira mais abundante na área estudada. O tamanho das moitas foi maior no fundo de vale, porém, os parâmetros mensurados para verificar a estrutura de comunidade, assim como a abundância dos indivíduos de G. gamiova não apresentaram diferença significativa (p > 0,05) entre os hábitats. Não houve nenhuma correlação significativa (p > 0,05) entre o número de estipes por moita e as variáveis microclimáticas e edáficas mensuradas. O crescimento dos estipes, avaliado pelo aumento do número de nós, apresentou diferença significativa, sendo maior.... (Resumo completo, clicar acesso eletrônico abaixo)

Avaliação da auto-suficiência energética de reatores anaeróbios no tratamento de esgoto sanitário e de água preta

The purpose of this project is to verify whether anaerobic reactors applied to sewage treatment are energetically self-sufficient. This evaluation can be made by balancing the methane produced through the anaerobic transformation stages (hydrolysis, acidogenesis, acetogenesis and methanogenesis) and the reactor energy consumption requirements. The original project included methanogenic activity tests, which could not be performed due to setbacks in the installation of an analytical instrument. Scientific articles about bench- and full-scale anaerobic reactors were investigated instead. An average substrate-to-methane conversion efficiency of 58,2±18,6% was found for the bench-scale reactors and higher efficiencies (89,2%) were found for the cases which had higher Organic Loading Rates (OLRs) values. The average energy output was 0,013 kWh/Lsewage, value unable to meet the energy needs for the reactor operation, considering equipments normally used such as temperature controller. This balance can become positive if few hypotheses are made, for example (i) to eliminate the use of temperature controller (ii) to alter the operation pattern from continuous to intermittent. Based on energy balance assessment of eight bench-scale reactors, it was observed that the implementation of a system for biogas utilization is not energetically feasible. However, interesting results were found for a full-scale sewage treatment plant, ETE Ouro Verde – Foz do Iguaçu, PR, Brazil. Even though its substrate-tomethane conversion efficiency was about 10% only, the energy balance was quite positive, with energy consumption of 68 kWh/month and energy production of 660 kWh/month. This analysis leads us to conclude that energy recovery from full-scale sewage treatment plants should be practiced by other plants