Differential distribution of sperm subpopulations and incidence of pleiomorphisms in ejaculates of captive howling monkeys (Alouatta caraya)

The aim of this study was to develop an objective method to determine the incidence of pleiomorphisms and its influence on the distribution of sperm morphometric subpopulations in ejaculates of howling monkeys (Alouatta caraya) by using a combination of computerized analysis system (ASMA) and principal component analysis (PCA) methods. Ejaculates were collected by electroejaculation methods on a regular basis from five individuals maintained under identical captive environmental, nutritional, and management conditions. Each sperm head was measured for dimensional parameters (Area [A, (square micrometers)], Perimeter [P, (micrometers)], Length [L, (micrometers)], and Width [W, (micrometers)]) and shape-derived parameters (Ellipticity [(L/W)], Elongation [(L - W)/(L + W)], and Rugosity [(4 pi A/P-2)]). PCA revealed two principal components explaining more than the 96 % of the variance. Clustering methods and discriminant analyzes were performed and seven separate subpopulations were identified. There were differences (P < 0.001) in the distribution of the seven subpopulations as well as in the incidence of abnormal pleiomorphisms (58.6 %, 49.8 %, 35.1 %, 66.4 %, and 55.1 %, P < 0.05) among the five donors tested. Our results indicated that differences among individuals related to the incidence of pleiomorphisms, and sperm subpopulational structure was not related to the captivity conditions or the sperm collection method, since all individuals were studied under identical conditions. In conclusion, the combination of ASMA and PCA is a useful clinical diagnostic resource for detecting deficiencies in sperm morphology and sperm subpopulations in A. caraya ejaculates that could be used in ex situ conservation programs of threatened species in Alouatta genus or even other endangered neotropical primate species.

Singular Value Decomposition and Ligand Binding Analysis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Gestão de estoque no setor de varejo calçadista: abordagem via análise multivariada e teoria do controle ótimo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pre-extrusion aromatization of a soy protein isolate using volatile compounds and flavor enhancers: Effects on physical characteristics, volatile retention and sensory characteristics of extrudates

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Abordagem multivariada na análise de diversidade genética em cruzamentos para alto teor de óleo em soja

The soybean crop is considered a high expression around the world. In plant breeding programs, knowledge of genetic diversity is extremely important and in this context, are frequently used multivariate analyzes. Thus, the aim of the present study was to evaluate the genetic divergence between soybean crosses through multivariate techniques. In total, 16 crosses were evaluated, which were in the F2 generation of inbreeding. The evaluated characteristics were plant height at maturity, height of the first pod, number of branches per plant, number of pods per plant, number of nodes per plant, hundred seed weight, grain yield and oil content. For the analyzes was used Euclidean distance, methods of hierarchical clustering UPGMA and Ward and principal component analysis. Genetic distances estimated using Euclidean distance ranged from 1.24 to 8.13, with the smallest distance observed between crosses C1 and C4, and the greatest distance between the C2 crosses and C6. The methods UPGMA clustering and Ward met crossings in five different groups. The principal component analysis explained 86.2% of the variance contained in the original eight variables with three main components. The APM characters, NV, NR, NN, PG% and oil were the main contributors to genetic divergence among traits. Multivariate techniques were crucial to the analysis of genetic diversity, and the methods of Ward and UPGMA clustering and principal components have consistent results in this way, the simultaneous use of these tools in genetic analysis of crosses is indicated

Caracterização hidroquímica do sistema aquífero Tubarão no município de Americana/SP

The growing interest in the use of groundwater resources is directly related to the economic advantages that the groundwater exploitation offers when compared to surface waters. This happens especially in large urban centers, such as the city of Americana / SP, where the rivers are increasingly contaminated by household and industrial waste. Therefore, this study aimed to characterize the Tubarão Aquifer System, in the city of Americana, to identify and evaluate the spatial distribution of different hydrogeochemical facies as well as understand the rock-fluid interaction through the construction of a conceptual hydrogeochemical model. This study was made based on the recognition of the possible chemical reactions that print the chemical characteristics of groundwater in the area. To do the job, there were two water sampling campaigns of all deep wells used by the City of Americana public water supply. From the results of hydrochemical, classification of water was made by Piper and Stiff diagrams as well as geostatistical data using cluster analysis of principal components. Based on information from the profiles obtained from the survey SIAGAS as well as in geological profiles provided by the city of Americana, we sought to detail the subsurface geology of the Subgroup Itararé in the city of Americana. The results obtained allowed the identification of three hydrochemical types in the study area: Bicarbonated calcium-sodium (1), bicarbonate sodium (2) and sodium chloride (3). The waters have bicarbonate alkaline pH to alkaline and can be considered weakly saline, with electrical conductivity values of around 161 mS / cm. Samples classified as sodium bicarbonate average of 174.99 mS / cm. The pH values ranging from 6.74 to 7.99, averaging 7.52. For the group of waters classified as sodium chloride, conductivity average is 164.32 mS / cm and pH values ranging... (Complete abstract click electronic access below)

Definição de um modelo de análise de sucesso das empresas baseado em índices contábeis e na análise multivariada

The objective of this paper is to relate the set of financial ratios that are directly related to the success of public traded companies using a methodological approach and the method of multivariate principal component analysis. This study consists in the use of profitability ratios, debt and liquidity, to define the relationship between financial ratios with the best public traded companies listed in the magazine Exame Melhores e Maiores of 2013. Multivariate analysis was used to reduce the dimensionality of multivariate data, making linear combinations of the original variables (financial ratios) and express the data in principal components that result in new variables that contains much of the original data. As a result, we got the optimal number of five principal components, and both represent 95.6% of the original data. Among of all financial ratios, we can highlight the direct relationship between profitability ratios for the first principal component, and the direct relationship between the liquidity ratios, both inversely related with non-capital participation rates and degree indebtedness to the second principal component

Aplicação da análise fatorial em cidades do Vale do Paraíba considerando os objetivos do milênio

Pós-graduação em Engenharia Mecânica - FEG

Estratégias de seleção de genótipos de soja por meio de modelos mistos e abordagens multivariadas

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Rastreabilidade da cama de aviário por isótopos estáveis em bovinos leiteiros

Pós-graduação em Zootecnia - FMVZ

Evaluation of the Atibaia River water quality using Lecane bulla as a test organism

In view of the diversity of environments found in the Brazilian territory, it is understandable that the use of native species can provide more relevant information for ecotoxicological studies. The purpose of this work was to evaluate the quality of water samples from the Atibaia River in an area that is under the influence of petroleum refinery using a native test-organism and submitting the data to PCA statistical analysis. Therefore, acute toxicity assays with Lecane bulla (Rotifera) were performed in four locations of the river, as well as physical-chemical analyses. Sampling was drawn in the dry and rainy seasons. The bioassays were static and lasted 48 hours; dead organisms were quantified at the end of the tests. Toxicological differences among the samples/per location and control were compared by means of the Analysis of Variance. Physical-chemical and mortality variables were simultaneously analyzed by multivariate analysis of the principal components and the Pearson correlation coefficient. Water samples from the exit of the refinery stabilization pond (location S.1) were toxic to L. bulla in both seasons, with significant differences in relation to the control and between the seasons. The statistical treatment of data showed that mortality was strong and positively correlated with total hardness, chlorides and EC, which together with pH presented higher values in location S.1, in the dry and in the rainy seasons. Due to its sensibility to the quality of the Atibaia river water samples, the potential use of L. bulla for ecotoxicological studies as an alternative test organism could be demonstrated.

Prediction of genomic-enabled breeding values and genome-wide association study for feedlot average daily weight gain in Nelore cattle

Pós-graduação em Genética e Melhoramento Animal - FCAV

Genome-wide association study and ancestral origins of the slick-hair coat in tropically adapted cattle

The slick hair coat (SLICK) is a dominantly inherited trait typically associated with tropically adapted cattle that are from Criollo descent through Spanish colonization of cattle into the New World. The trait is of interest relative to climate change, due to its association with improved thermo-tolerance and subsequent increased productivity. Previous studies localized the SLICK locus to a 4 cM region on chromosome (BTA) 20 and identified signatures of selection in this region derived from Senepol cattle. The current study compares three slick-haired Criollo-derived breeds including Senepol, Carora, and Romosinuano and three additional slick-haired cross-bred lineages to non-slick ancestral breeds. Genome-wide association (GWA), haplotype analysis, signatures of selection, runs of homozygosity (ROH), and identity by state (IBS) calculations were used to identify a 0.8 Mb (37.7-38.5 Mb) consensus region for the SLICK locus on BTA20 in which contains SKP2 and SPEF2 as possible candidate genes. Three specific haplotype patterns are identified in slick individuals, all with zero frequency in non-slick individuals. Admixture analysis identified common genetic patterns between the three slick breeds at the SLICK locus. Principal component analysis (PCA) and admixture results show Senepol and Romosinuano sharing a higher degree of genetic similarity to one another with a much lesser degree of similarity to Carora. Variation in GWA, haplotype analysis, and IBS calculations with accompanying population structure information supports potentially two mutations, one common to Senepol and Romosinuano and another in Carora, effecting genes contained within our refined location for the SLICK locus.

Estrutura da população e epidemiologia de Moniliophthora roreri no Magdalena Medio Colombiano

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seleção de genótipos de soja portadores ou não do gene RR por meio de análise multivariada e desempenho agronômico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)