Estudo das células primárias dentais, viabilidade celular e bioatividade de materiais á base de silicato de cálcio

Pós-graduação em Odontologia - FOAR

Características da base de conhecimentos de professores no ensino de biologia celular a partir de um curso de formação continuada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Artemidia independente: Vidas Imperfeitas, o quadrinho em um diálogo entre linguagem e suporte

The term “independent media art” expresses an artistic-scientific process performed in the Art- Science Communication interface presented as Final Paper in Visual Arts baccalaureate modality. The first part consists in gather, in artisticscientific research, an overview of the production of independent comics, mainly in Brazil, as well as some of the manifestations that were derived from it. The identification is made from bibliographical and iconographical survey, both printed and virtual materials. The second part discusses the production of a comic of my own - Imperfect Lives - published independently. This work is inserted in the line of research “Artistic Processes and Procedures” of the Department of Fine Arts of the Art Institute of UNESP and describes: Creative process, production, dissemination and influences. The third and final section brings together the work done by me during the four-year course, whose theme has some relation with comics. Experiments are in various media, using one or another characteristic intrinsic to them. The monography includes as an appendix a copy of each issue of the fanzine Imperfect Lives. The methodology used was Freinetiana Educational Cybernetics, developed in the research group “Art media and Videoclip”, whose leading advisor is the one from this Final Paper. The result and discussion of the artistic research and scientific monography were reported in the following versions: PDF File repository for dissemination in the virtual library of the Institute of Arts. Hardcover version for physical collection in the Library of the Institute of Arts. Paper version for the committee. Template version appropriate for submission to International Scientific Congress in the area of Arts

Indução de morte celular por apoptose decorrente da irradiação com Raios-X: um ensaio com células mamárias humanas tumorais

Apoptosis is a form of programmed cell death selectively removes abnormal cells, and thus contributes to maintaining the balance of the dynamics of cell reproduction. Therefore the verification of the occurrence of apoptotic cell death after a pathological stimulus is crucial for the analysis of the maintenance of normal cell cycle of a given tissue or organ. In this experiment were used cells lines human mammary tumor MDAMB231, T47, MCF7, which were irradiated with X-rays at a dose of 5 Gy in a time interval of 15 seconds, and filtration of 1mm aluminum. Samples containing the cells were grown in a specific culture medium, containing fetal bovine serum and growth factor, and two samples were prepared with each of the cell lines, one to be irradiated, and another that has not been irradiated, which denoted by negative control of the irradiation. The primary goal of the experiment was to verify and compare the rates of apoptosis in each cell lines, in which were irradiated and that were not irradiated, using flow cytometry as a method for detecting apoptotic cell death in together with specific markers annexin V and propidium iodide. Data from the readings made by flow cytometry were analyzed and interpreted using the software WinMDI statistical graph. By comparing the indices relating to the readings of positive and negative for specific markers of apoptosis, based on differences in the statistical data presented lectures regarding the cellular irradiated and not irradiated, collude cells in question once... (Complete abstract click electronic access below)

Análise da expressão protéica de moléculas de adesão celular em melanomas cutâneos

Cutaneous melanoma is a malignancy caused by the uncontrolled proliferation of melanocytes. Has been increasing in incidence and mortality over the years. This type of tumor may arise in the skin or any other place where there was migration of neural crest cells. Melanocytes are located in the basal layer of the epidermis forming the epidermal melanin unit, involving a melanocyte to 36 keratinocytes. The proliferation of melanocytes depends on this relationship with the keratinocytes, gap junctions, E-cadherin and desmoglein. The basal membrane is responsible for promoting the integrity of the skin, their adhesion structures shown to be altered in several skin diseases. Some molecules that play the role of adherence in this membrane are the laminins, type IV collagen and type XVII and perlecan. Analysis of protein expression of cell adhesion molecules in melanoma by immunohistochemistry on tissue microarray (TMA). We selected paraffin blocks of patients who were diagnosed with cutaneous melanoma in the period 1995 to 2010, reaching a sample of 124 cases. All these have gone through review of preparation for subsequent histological analysis of tissue microarray (TMA). The protein expression will be analyzed by immunohistochemistry, using markers anti-laminin, anti-collagen type IV and type XVII and anti-perlecan. There was a predominance of female patients, Caucasians and southeastern Brazil. There were frequent in type of extensive superficial ulcers with Breslow depth greater than 4 mm, Clark level IV and mitotic index greater than 6 mitoses per cga. The patients, most of them were female with mean age of 57.13 years and Caucasian. The most frequent histological type was extensive superficial followed by acral lentiginous. Predominated cases very deep, with Breslow thickness greater than 4 mm, Clark IV, ulcerated and on average by 9 cga mitosis, leading to worse prognosis in this sample. It...(Complete abstract click electronic access below)

Desenvolvimento de equipamento LED para aplicação em cultura celular

O estágio realizado em parceria entre o Departamento de Física e Biofísica do IBB-UNESP e o Hemocentro de Botucatu da FMB-UNESP teve como objetivo desenvolver um novo equipamento de luz LED capaz de interagir com culturas celulares. Desta forma foi desenvolvido um equipamento com luz azul e luz vermelha. A luz azul é responsável por inibir o crescimento celular, a luz vermelha é responsável por aumentar o crescimento celular. O equipamento consiste de uma fonte, e dois conjuntos com três LEDs cada. A cultura celular escolhida foi a de Miemola Murino (NS1), uma linhagem que possui alta taxa de crescimento e que foi mantida em estufa e meio de cultura, (simulando condições normais de sobrevivência), sendo irradiada três vezes ao dia. As amostras foram coletadas e contadas em câmara de Neubauer. Os resultados obtidos, quando comparados com o controle, não apresentaram significativa diferença de crescimento quando irradiados com luz LED vermelho. Porém quando comparados o grupo controle com o grupo irradiado com LED azul, notou-se uma significativa redução da quantidade de células a partir do sétimo dia de irradiação. Acredita-se que a ineficácia dos LEDs vermelhos ocorreram pois utilizamos células com metabolismo bastante elevado, dificultando um aumento ainda maior da taxa de crescimento. A redução da população de células azuis ocorre pois a radiação interage com a membrana celular, causando a quebra dessa membrana e conseqüente morte da célula. Outra via para a morte é a lesão causada no DNA das células, que ocorre quando a luz azul é absorvida pela molécula

Cultivo celular in vitro: importância para a pesquisa biomédica e dimensão da problemática de autenticação de linhagens celulares

Experimental models composed by human and animal cell lines are simplified and informative, allowing them to be widely used for biomedical research. Most laboratories that use in vitro cultivated cells maintain a variation of cell lines stored and cultivated. Therefore, misidentification and cross-contamination events can happen during cell lines handling. This problem can generate a repertoire of dubious results and papers, which may prejudice biomedical research. Recently it was created the International Cell Line Authentication Committee (ICLAC), which aims to spread knowledge about cross-contamination and misidentification of in vitro cell lines. Despite of the efforts spent trying to aware scientific community about the importance of the correct identification of cells, the number of papers based on misidentified cell lines it´s still worrying, compromising the reliability of out coming results and conclusions regarding them. The present study aims to analyze and discuss the main advantages and limitations of eukaryote in vitro cell lines use, characterizing the cell lines authentication problems. Therefore, compilation and critical analyses of literature data was realized, aiming to improve the understanding about this subject. Based on information about 445 cell lines with issues published by ICLAC it´s clear that contamination in human cell lines represented 89,2 % of mentioned problems. HeLa cell line was the responsible for most contamination, especially in 92 normal tissue cell lines, representing 44,6% of the contamination. These results reinforce the importance of periodic maintenance of cell lines cultures by labs and implementation of authentication methods as polymorphic STRs, besides obtaining cell lines from reliable sources and cell banks

Influência da luz LED na cultura celular de Mieloma múltiplo murino–NS1 in vitro

A monoclonal antibody (mAb) is an important tool in medical biotechnology and the production of biopharmaceuticals, especially for disease diagnosis and treatment of infections, because the antibodies have a significant advantage over chemical agents used in conventional therapies . The last thirty years the technology of production of monoclonal antibodies developed mainly the technique of obtaining in vitro, but also of their production is laborious, the cost is high. A major element of the high cost of production is the fact that the long-term culture consumes a large amount of imported inputs with high added value. A major contribution of this work is to promote cell growth more quickly and efficiently. Currently, a great race to discover new technologies and techniques to synthesize new antibodies and significantly increase the production of murine mAbs. New technologies such as laser and LED are innovations and widespread in modern life, so much so that its use has proliferated worldwide, primarily in the medical field. Recent studies show a series of results from the influence of the LED light in biological tissues such as: increasing the rate of cell proliferation, increased production rate of fibroblasts, increasing the rate of synthesis of RNA and DNA synthesis of ATP, etc. To assess the contribution of the LED in the culture of Myeloma NS1murino compared to the standard procedure. - NS1 cells were provided and followed the criteria of culture medium of the Laboratory of Cellular Engineering Center of Botucatu (POPs). The same amount of cells was grown in bottles of 25 cm2 polystyrene Tissue Culture Treated, specifically marked and kept in special medium RPMI 1640 Gibco BRL  supplemented with fetal bovine serum 10%, essential amino acids and non-essential, glucose, insulin and antibiotics. It was used in LEDs Cromatek wavelength of 630nm, 475nm and 530nm. The groups were... (Complete abstract click electronic access below)

Relações públicas e a mediação do diálogo intercultural na comunicação organizacional

The large flow of businesses going abroad generates an ever more diverse internal multicultural organizational scenario. Different national cultures inside an organization can directly influence the management of people. Human values, languages, customs, work modes/routines and different habits can create conflicts among parties. This study deals with the role of Public Relations as a tool/strategy to deal with conflicting intercultural communication inside business organizations. The analysis is grounded on theoretical principles concerning the roles of communication professionals as the individuals responsible for the relationship between an institution and the internal public. The study introduces intercultural communication as a growing area to be explored by the Public Relations professional and highlights the possibility of emerging innovative solutions for organizational problems. It also brings reports by professionals that have intercultural experience concerning Brazil and Germany in an attempt to illustrate conflicts that might have been prevented by actions taken by a specialist in Communication in order to promote mutual understanding

Sobre a questão da autonomia na prática docente com professores e professoras: leitura e diálogo

O estudo busca, num ato comprometido, aproximar-se das questões da autonomia na prática docente a partir do diálogo com autores(as) e com professoras(es) da rede pública de Ensino Básico com vistas no repensar da noção de autonomia na prática docente. Na primeira parte, familiarizo-me com o cenário no qual estamos inseridos: influências da globalização e da política neoliberal sobre as políticas educacionais brasileiras, como nos discursos dos PCNs e os currículos oficiais. Em seguida, dialogo com alguns autores sobre o que se compreende por autonomia e as questões da autonomia na prática docente. Com eles, vou descobrindo afinidades. Na segunda parte, em busca do diálogo com e entre professoras(es), organizei um grupo de discussão por meio de um curso. O Curso de Difusão Cultural - Leituras de Paulo Freire: autonomia e subjetividade - foi um espaço criado no intuito de fomentar o diálogo, a reflexão e a troca de saberes entre professoras(es) e alunas(os) de licenciatura a partir de escritos de Paulo Freire. A escolha dos textos pautou-se no entendimento destes como disparadores de reflexões que pudessem acercar-se do tema em estudo. Cada encontro apresentava uma leitura antecipada na qual sugeri que destacassem trechos a serem compartilhados e discutidos com os demais colegas no encontro seguinte. Durante as leituras, para cada texto, propus que escrevessem algo relacionando os trechos destacados com suas práticas docentes; nomeei esta atividade de produção escrita. Após o término dos encontros, os materiais de registros – gravações em audiovisual, produções escritas e anotações em caderno de registros – foram analisados. Nota-se que, mesmo diante de tantas incoerências de uma política educacional que pouco promove a legitimação de seu discurso, a autonomia na prática docente, como a compreendo neste estudo, não pode ser exclusivamente dada nem... (Resumo completo, clicar acesso eletrônico abaixo)

Avaliação da proliferação celular na próstata de gerbilos vasectomizados, após indução química pela injeção intraperitoneal do carcinógeno N-metil-N-nitrosuréia (MNU)

Prostate cancer is the second leading cause of cancer death in the country. Due to this factor, the interest of showing what relationship exists between vasectomy surgery and the incidence of prostate carcinoma has started. Some epidemiological studies showed an increased risk of prostate cancer in vasectomized men (Emard et al., 2001). On the other hand other authors have argued that there is no correlation (Patel et al., 2005) and there are those who said that vasectomy is linked to reduced risk of prostate cancer (Ross, 1983). Faced with an analysis of published works, such discussion remains today. The vasectomy, or deferentectomia, is a contraceptive male method, which is the section of the vas deferens of man by preventing the sperm from being expelled along with the seminal fluid during ejaculation, and is one of the most simple, economical, uncomplicated post- operative. This study aims to evaluate the process of cell proliferation and to evaluate immuno-histochemically the expression of specific markers of PCNA (Proliferating Cell Nuclear Antigen) and Ki-67, in the prostate of the gerbil after chemical induction by intraperitoneal injection of the carcinogen N-methyl -N-nitrosourea (MNU), because the regulation of the functional balance between cell proliferation and apoptosis is associated with hyperplasia and prostatic carcinoma. The experimental procedure was performed at the Laboratory of the Department of Anatomy, along with collecting the data for later analysis

Interferência eletromagnética em equipamentos de diatermia por microondas por aparelhos telefonia móvel celular GSM

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Proliferação e Morte Celular (Apoptose) avaliadas em ratos Lewis após tratamento com mistura de praguicidas em baixas doses

The use of pesticides in Brazil has intensified over the years. And since 2009 it was ranked as the largest market for pesticides worldwide. The consequential diffuse contamination of the environment is therefore due to the increasing number of chemicals of different classes, origins and modes of action. Little is known about the action of pesticides on human health in situations of co-exposure. Possible toxic effects are not restricted to agricultural and industrial workers, but also the general population that may be exposed continuously to its residues in food and water. Although these pesticides are mostly present in the environment at low doses, it must be considered that possible cumulative or synergistic effects may occur when there are concurrent or sustained exposure for two or more of these agents, which can lead to late manifestation of subclinical damages, sometimes irreversible. Thus, the specific objective of this study was to assess the effect of carcinogenesis promotion of a mixture of pesticides at low doses and analyze the phenomena of cell proliferation and apoptosis in rat liver. A total of 50 male Lewis rats was separated into 5 groups for 8 weeks in a medium term hepatocarcinogenesis model. The three different classes of pesticides (dieldrin, dicofol, endosulfan, dichlorvos and permethrin), whose residues were detected by ANVISA during the period from 2001 to 2005 in tomatoes cultures, were added to the feed of rats initiated to hepatocarcinogenesis with diethylnitrosamine (DEN- 200mg/kg ip). We used two different mixtures, one with no toxic effects at doses (MEX1) referring to the NOEL (no-observed-effect level) and another at doses LOEL / LEL / LOAEL (Lowest-observed-effect level / Lowest-effect level / Lowest -observed-adverse-effect level), to the installation of adverse effects (MEX2), derived from chronic studies. All animals ...(Complete abstract click electronic access below)

Significados da reforma agrária em bairros periféricos de Presidente Prudente (SP): uma tentativa de diálogo entre a luta pela Terra e a soberania alimentar

La lucha por la tierra en el Brasil se confunde con la própria historia del país, erigida sobre una cuestión agraria turbulenta en la cual ha predominado la hegemonía de los grandes proprietarios de tierra, tanto en la forma de latifundistas, cuanto, más recientemente, de los empresarios del agribusiness, caracterizando una versión más moderna de la dominación de la lógica del capital en detrimento de las demás formas de organización de la vida. A partir de la segunda mitad del siglo XX, ocurre un aguzamiento de los conflictos por la tierra con la emergencia de los principales movimientos sociales en Brasil, como el MST. Esos movimientos surgen en un contexto de transformación de la formación socioespacial brasileña, marcada por la creciente urbanización del país, lo que repercute em las sus formas de organización, pasando a comparecer una preocupación con la población urbana en las discusiones y acciones en pro de la Reforma Agraria. Yuxtaponiendo-se a eso proceso, también ha emergido fortemiente en las últimas décadas la problemática de la hambre en el mundo, tanto a través de los dados alarmantes de los organismos supranacionales (FAO), como del debate impulsado por los movimientos sociales acerca de la Soberanía Alimentaria (Via Campesina). Todos esos elementos merecen atención em esto trabajo, que busca aprehender cómo se efectiva la participación de barrios de la periferia pobre de la ciudad de Presidente Prudente/SP en el proceso de lucha por la tierra en el Pontal del Paranapanema, haciendo eso por lo medio de una lectura geográfica de esa realidad que prime por la superación analítica de la disociación entre ciudad y campo, como se fueran espacios inertes entre sí. Además, es nuestro objetivo destacar las motivaciones que llevan los sujetos protagonistas de eso proceso a dejaren la ciudad y adhieriren a la lucha por la tierra, permitiendo identificar elementos que extrapolan la esfera económico-material

Biomateriais e terapia celular na reparação da cartilagem articular em equinos

Articular cartilage is the structure that coats the bone ends in regions where two bones are articulated, allowing movement. It has inefficient intrinsic and extrinsic mechanisms of repair, usually resulting in fibrocartilage formation after injury. Such repair have lower strength, stiffness and usability features when compared to hyaline cartilage. The mesenchymal stem cells have the potential to regenerate tissue without the production of scar, and because of this feature it is well studied. But to have its maximum chondrogenic potential, it is necessary to use scaffolds and growth factors. Biomaterials play the role of scaffold for the cells allowing them to become attached, grow and produce extracellular matrix, leading to formation of repair with hyaline cartilage. In this sense, the purpose of this study is to provide information on the various studies using cell therapy and / or biomaterials to produce hyaline cartilage