Avaliação in vitro de diferentes métodos de análises de fungitoxicidade de óleos essenciais

Environmental problems caused by synthetic fungicides have increased the search for alternative methods of control of plant diseases. The objective was to evaluate the effect of essential oil of citronella grass, on the fungus Rhizoctonia solani, in different methods of in vitro fungitoxicity. We used a randomized design in a factorial design with four replications, where the factors were composed of four methods for assessing the in vitro fungitoxicity of the essential oil of citronella grass (essential oil diluted in Tween 80 (0.5%) and embedded in the culture medium PDA (potato dextrose agar) still melting, essential oil diluted in Tween 80 (0.5%) and distributed on the surface of the PDA; oil essential diluted in Tween 80 (0.5%) and distributed on filter paper attached to the inner surface of the lid of the Petri dish, pure essential oil and distributed on the surface of the culture medium, and control) and five evaluation periods (2, 4, 6, 8 and 10 days of incubation). Was used 0.25μL mL-1 of citronella oil in all treatments. Of the treatments evaluated the use of pure oil distributed on the surface of the culture medium was more effective in reducing the mycelial diameter in all evaluations. In this method the rate of mycelial growth was 9,02 mm day-1, reaching in last evaluation 79,77 mm.

Sensibilidade de stenocarpella macrospora a fungicidas

The application of fungicides in the aerial organs is control strategy to macrospora spot caused by fungus Stenocarpella macrospora. The objective of this study was to determine the sensitivity of S. macrospora to fungicides by inhibition of mycelial growth (MG) and conidial germination (CG). It was eval uated 12 fungicides belonging to the chemical groups of the benzimidazoles, triazoles and strobilurins, six concentrations and two isolates of the fungus (SC and MT). The fungicides were diluted in sterile distilled water and added to the culture medium of potato dextrose agar (mycelium) and water-agar (spore) after sterilization. The percentage of inhibition of MC and CG was calculed in comparison with control, estimating of 50% inhibitory concentration (IC50). The fungicides tested were effective in inhibiting the MC. The IC50 was less than 1 ppm for all fungicides. There was no difference between isolates. The inhibition of CG had higher fungitoxicity strobilurins, and the IC50 was between 0.0035 and 0.03 ppm, and the isolated SC showed the higher sensitivity to the fungicides. The IC50 values obtained for fungicides and specific S. macrospora will be useful in monitoring the sensitivity of the fungus, especially in regions with intense demand for fungicides in corn.

Fusarium solani f. sp. passiflorae: A new forma specialis causing collar rot in yellow passion fruit

The aim of this study was to characterize a Fusarium population obtained from yellow passion fruit (YPF) with collar rot using pathogenicity, morphocultural characteristics and molecular tests. Pathogenicity and disease severity were assessed in six plant species: YPF, zucchini, tomato, bean, soya bean and cucumber. Potato dextrose agar medium (PDA) was used to determine mycelial growth at five temperatures (15-35°C). The colour produced by isolates was also determined on PDA at 25°C. Synthetic nutrient agar medium was used to evaluate: (i) type of mycelium and phialides; (ii) size, shape and number of septa from conidia; and (iii) production of chlamydospores and perithecia. Molecular tests consisted of sequencing the ITS-5·8S rDNA region and elongation factor 1α (EF-1α) gene. The isolates caused large lesions on YPF, zucchini and tomato, with YPF having the highest mean disease severity and being the only one that showed wilt symptoms and death of the plant. Thus the isolates showed host specificity. Maximum mycelial growth occurred at 25°C and the predominant colour was bluish-white. The isolates produced long phialides, dense aerial mycelium, oval microconidia with a mean size of 9·5 × 2·6 μm, macroconidia of 32·7 × 3·4 μm with 3·3 septa, and chlamydospores; only one isolate lacked perithecia. Phylogenetic trees of the ITS region and EF-1α gene showed that isolates from YPF formed a distinct group within the F. solani group and the formae speciales of F. solani. It is proposed to name all isolates from YPF as F. solani f. sp. passiflorae. © 2013 British Society for Plant Pathology.

Análise de imagens no estudo da porosidade em latossolo cultivado com batata (Solanum tuberosum L.) sob dois sistemas de irrigação

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Crescimento, produtividade, acúmulo e exportação de nutrientes em cultivares de batata (Solanum tuberosum L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação das características agronômicas, dos distúrbios fisiológicos, do estado nutricional da planta e da qualidade dos tubérculos em cultivares de batata (Solanum tuberosum L.)

Pós-graduação em Agronomia (Agricultura) - FCA

Estresse hídrico e adubação silicatada em batata (Solanum tuberosum L.) cv. Bintje

Pós-graduação em Agronomia (Agricultura) - FCA

Características bioquímicas em cascas, folhas e talos de vegetais pós-colheita em sistema de produção convencional e orgânico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Adubação com manganês em soja: efeitos no solo e na planta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morfometria da bacia hidrográfica do rio Bobo no Estado de Nariño, Colômbia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de ciclodextrina glicosiltransferase (CGTase) e de ciclodextrinas como coadjuvante na inibição do escurecimento em alimentos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Características físico-químicas e estruturais de amidos nativos e suas dextrinas Naegeli

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Características estruturais e físico-químicas de amidos obtidos de diferentes fontes botânicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aplicação de silício solúvel via solo e foliar na cultura da batata (Solanum tuberosum l.) sob deficiência hídrica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de cinco linhagens de Agaricus Bisporus Lange (Imbach) (“champignon de Paris”) em diferentes formulações de composto e meios de cultura

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)