Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles

Photodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5 mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model. Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers.

Efeito de diferentes terapias locais para o tratamento de periodontite crônica em indivíduos diabéticos tipo 2: estudo clínico controlado randomizado triplo cego de boca dividida com acompanhamento de seis meses

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Quimiluminescência como fonte de luz alternativa na terapia fotodinâmica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo comparativo do uso da terapia fotodinâmica antimicrobiana (aPDT) com uso sistêmico de amoxicilina e metronidazol, no tratamento da periodontite crônica em pacientes fumantes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Associação de antibióticos e terapia fotodinâmica antimicrobiana para o controle de Acinetobacter baumannii

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Modificação em fotossensibilizador e sua aplicação na terapia fotodinâmica antimicrobiana em substrato dentinário

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da luz visível associada à ftalocianina de cloro-alumínio na inativação da Borrelia anserina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nonsurgical Periodontal Therapy Combined with Laser and Photodynamic Therapies for Periodontal Disease in Immunosuppressed Rats

Background. Periodontal disease is often associated with systemic diseases and is characterized by destruction of the tissues supporting the teeth. Patients using immunosuppressive drugs such as tacrolimus are among those who suffer from tissue destruction. Objective. We sought to evaluate the effects of laser and photodynamic therapies (PDT; nonsurgical) as an adjunct to scaling and rootplaning (SRP) in the treatment of corona-induced periodontitis in rats immunosuppressed with tacrolimus (Prograf).Materials and Methods. The animals were divided into 5 groups. Each groups had 6 rats. Group I, the control group, received only saline solution throughout the study period of 42 days and did not receive periodontal treatment; group II received saline solution and SRP; group III received tacrolimus (1 mg/kg per day) and was treated with SRP; group IV animals were treated identically to group III and then administered laser treatment; and in group V, the animals were treated identically to group III and then administered PDT.Results. Statistical analysis indicated decreased bone loss with the progression of time (P = .035). There was no difference between the bone loss associated with the types of treatment administered to groups I, II, and III (P > .9) or groups IV and V (P > .6). The analysis also indicated that immunosuppression was not a bone loss-determining factor.Conclusion. Laser and PDT therapies were effective as an adjunctive treatment to SRP in reducing bone loss caused by experimental periodontitis induced in animals being treated systemically with tacrolimus.