Estudo do potencial de degradação do herbicida diuron e seus metabólitos por bactérias isoladas de solo de canavial

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Degradação do herbicida diuron por bactérias isoladas de solos de cultura de cana-de-açúcar e avaliação da toxidade dos metabolitos gerados sobre peixes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificação de bactérias do filoplano de citros capazes de interromper o quórum sensing em Xanthomonas citri subsp. citri

Pós-graduação em Microbiologia Agropecuária - FCAV

Percepción de un grupo de mujeres acerca del hecho de ser portadoras del VPH

Background: The possibility to acquire sexually transmitted diseases generates bio-psycho-social conflicts affecting daily life of women. Objective: To investigate the knowledge of women on the concept of being infected by the human papilloma virus (HPV) and to understand the significance they attributed to their condition as HPV carriers. Material and method: It is a qualitative study in which ten women were interviewed, after the result of a cytopathological exam suggesting HPV. Data were collected at the Center of Health School of the Universidade Estadual Paulista -UNESP-Brazil. Results: The significant items were: deception and preoccupation about the cure, since they indicate that it is very deceptive to be infected with an incurable disease by a person whom they trust; the method of having sexual relations and being infected again; the necessity of care and of being optimistic to face the disease and the threat of cervix cancer originating anxiety. Discussion: Orientations made by health professionals were efficacious to clarify the relationship with cancer development. It is possible to understand the necessity of assistance orientations and the opportunity to hear them, offering individualized quality care. This study also offers important elements to reinforce the educational role of health professionals, principally those referring to sexually transmitted infection and cancer, seeking prevention and early treatment with the offered information. However, there is no desire of finishing the subject regarding perception, thus there is much to discover about diseases related to human papilloma virus. Key words: women carriers of HPV, feelings attributed by women.

A flow cytometry method for testing the susceptibility of Cryptococcus spp. to amphotericin B

Human fungal infections have increased at an alarming rate in recent years, particularly in immunocompromised individuals. Cryptococcosis is the second most prevalent systemic fungal infection worldwide, and the most prevalent systemic infection in immunocompromised individuals, representing more than 70% of cases. The incidence of cryptococcosis is high in people with HIV/acquired immunodeficiency syndrome (AIDS), with recent estimates indicating that there are one million cases of cryptococcal meningitis globally per year in AIDS patients. The aim of this research was to develop a rapid flow cytometric antifungal susceptibility test and to compare the results with the standard methods. A reference strain and clinical isolates of Cryptococcus neoformans and Cryptococcus gattii were tested for susceptibility to amphotericin B by flow cytometry using propidium iodide as indicator of viability. Flow cytometry (FC) results were compared with the minimum inhibitory concentration (MIC) values determined by microdilution. The antifungal activity of amphotericin B ranged from MICs of 0.06 to 2μg/ml for the 11 isolates studied. The same results were found by FC. The FC method allows same-day results, assisting in the selection of appropriate antifungal therapies. These results demonstrate an excellent correlation between FC and the classic methods of testing for susceptibility to antifungal agents. This rapid diagnosis method makes it possible to quickly administer effective therapeutic interventions, often saving lives.

Biomassa da bactéria Rubrivivax gelatinosus proveniente do tratamento de efluente de piscicultura melhora a saúde dos peixes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção de genes de entrotoxinas de Staphylococcus aureus e estafilococos coagulase negativos isolados de leite bovino e de tanques de refrigeração comunitários procedentes de pequenas propriedades da região de Bauru-SP

Pós-graduação em Medicina Veterinária - FMVZ

Fatores associados a qualidade do leite, higiene animal e concentração bacteriana na cama de vacas leiteiras confinadas no sistema de compostagem

Pós-graduação em Medicina Veterinária - FMVZ

Efeito de scaffolds de nanofibras incorporados com antibióticos sobre biofilmes formados por bactérias presentes nos canais radiculares

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Emprego de redes complexas para modelar e analisar a disseminação de microrganismos da familia enterobacteriaceae em pacientes mantidos em unidades de terapia intensiva

Nowadays the studies of different methodologies to interfere in the growing and spread of serious infections and systemic status in institutionalized patients those kept on intensive therapy units are relevant to understanding these complex systems and bring benefits to several health areas, particularly public health. In this study, it was analyzed the clinical and microbiological data from patients hospitalized in intensive therapy units. The interaction between patients and caregivers was modeled and analyzed using dynamic system model and complex network theory, identifying outbreaks values of microorganisms of Enterobacteriaceae Family.

Speech-language therapy for treatment of dysphagia and dysphonia in systemic granulomatous diseases: granulomatous diseases: dysphagia and dysphonia treatments

The granulomatous lesions are frequently founded in infectious diseases and can involve the larynx and pharynx and can cause varying degrees of dysphonia and dysphagia. There is still no systematic review that analyzes effectiveness of speech therapy in systemic granulomatous diseases. Research strategy: A systematic review was performed according to Cochrane guideline considering the inclusion of RCTs and quasi-RCTs about the effectiveness of speech-language therapy to treat dysphagia and dysphonia symptoms in systemic granulomatous diseases of the larynx and pharynx. Selection criteria: The outcome planned to be measured in this review were: swallowing impairment, frequency of chest infections and voice and swallowing symptoms. Data analysis: We identified 1,140 citations from all electronic databases. After an initial shift we only selected 9 titles to be retrieved in full-text. After full reading, there was no RCT found in this review and therefore, we only described the existing 2 case series studies. Results: There were no randomized controlled trials found in the literature. Therefore, two studies were selected to be included only for narratively analysis as they were case series. Conclusion: There is no evidence from high quality studies about the effectiveness of speech-language therapy in patients with granulomatous diseases of the larynx and pharynx. The investigators could rely in the outcomes suggested in this review to design their own clinical trials.

Avaliação do grau de conhecimento sobre Leishmaniose e Toxoplasmose em moradores do município de Araçatuba, SP

Considering the importance of zoonotic infections by Leishmania spp. and Toxoplasma gondii in Araçatuba region, São Paulo, this study determined the degree of knowledge of residents of Araçatuba about these two diseases. Questionnaires about these diseases were applied to 123 people. Regarding schooling, all the respondents were literate, and 69.9% (86/123) did not attend higher education and 30.8% (37/123) completed an undergraduate degree. Of these, 91.9% (34/37) knew the meaning of the term zoonosis, with significant difference compared to those who were not trained. This word was unknown by 57% (29/123) of total respondents. Most non-graduates, 59.3% (51/86) did not know what is toxoplasmosis and among graduates, 35.1% (13/37) ignored the subject.There were significant difference between these groups. Regarding the prevention of Leishmania infection, a greater proportion of the residents advocated environmental cleanup, the collar repellent and use of citronella. In relation to toxoplasmosis, 54.5% did not know the ways of transmission of this disease and among those with graduate degree, 13 ignoring any transmission medium. When it comes to prevention of this disease, 55.3% (68/123) did not know any prophylactic. In the group of higher education, 14 did not know how to prevent toxoplasmosis. The results of this study show that the majority of respondents are not aware about the mode of transmission of these diseases, especially in relation to toxoplasmosis.

Efeito do plasma rico em glicose na apoptose de neutrófilos de cães

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chronic osteomyelitis of the maxilla and mandible: microbiological and clinical aspects

Osteomyelitis of the mandible and maxilla are common in developing countries and their treatment may be long-standing and difficult. Thus, the aim of this study was to discuss the main biological aspects of the chronic osteomyelitis of the jaws of especial interest for dental team. These infections are associated with a complex microbiota composed mainly by anaerobic bacteria, sometimes associated with microorganisms originated from the skin and digestive tract. These data suggest that chronic osteomyelitis of the mandible and maxilla should be treated as anaerobic infections in most cases. In addition, local surgical treatments are relevant in the therapy outcome, associated to the use of antimicrobial agents, and the failure to accomplish them is a major cause of treatment failure.

Biossegurança na clínica de radiologia odontológica

In the practice of dental radiology, although there was no production of aerosols, there is the contamination of equipment and surfaces by contact with blood and saliva. This is making necessary the adoption infection control procedures to protect patients and staff. The objective of this paper is to present the procedures of prevention during the radiographic examination and radiographic processing in the darkroom. The proposed recommendations for the area of Dental Radiology include efficient cleaning and protection of equipment and surfaces, personal protective measures and to avoid contamination of the darkroom. These recommendations are obtained by simple procedures as cover films and radiological equipment with plastic barriers.