Comparação entre diversos substratos no enraizamento de estacas de crotón (Codiaeum variegatum L.)

Com o objetivo de determinar o melhor substrato para o enraizamento de estacas de crotón (Codiaeum variegatum L.), utilizou-se areia, vermiculita média, areia x vermiculita (75% x 25%); (50% x 50%); (25% x 75%), esponja fenólica e solo. O experimento foi conduzido em câmara de nebulização com delineamento em blocos casualizados com quatro repetições de vinte estacas cada. Determinaram-se as propriedades físicas dos substratos e foram efetuadas as seguintes avaliações: porcentagem de estacas enraizadas, porcentagem de estacas brotadas, número de raízes por estaca, comprimento da maior raiz e peso da matéria seca das raízes. Os resultados permitem concluir que o substrato vermiculita média é o mais indicado para o enraizamento de estacas de crotón, embora os demais, exceto o solo e areia que apresentaram os piores desempenhos, também possam ser utilizados.

Multiplicação in vitro DE Ficus carica L.: efeito da cinetina e do ácido giberélico

A cultura da figueira é afetada pelo vírus-do-mosaico e a cultura de tecidos é uma alternativa para se proceder à limpeza clonal. Neste trabalho, objetivou-se estudar o efeito da cinetina e GA3 na multiplicação in vitro da figueira. Segmentos nodais foram inoculados em meio de cultura WPM contendo as seguintes combinações de cinetina (0; 0,5; 1; 2 e 4 mg.L-1) e GA3 (0, 2, 4, 6 e 8 mg.L-1). Avaliaram-se número e comprimento dos brotos, peso da matéria fresca e seca da parte aérea, número de raízes, peso da matéria fresca e seca do sistema radicular e de calos. A utilização de 0,5 mg.L-1 de cinetina promoveu melhor taxa de multiplicação in vitro de Ficus carica. O GA3 reduziu a formação e multiplicação dos brotos e induziu ao estiolamento, à hiperidricidade, clorose e necrose apical das plântulas.

Influência de diferentes tipos de estacas e substratos na propagação assexuada de hortênsia [Hydrangea macrophylla (Thunb.) Ser.]

A hortênsia (Hydrangea macrophylla) é um arbusto semilenhoso de 1,0 a 2,5 m de altura, de folhagem e florescimento decorativos, muito utilizados como flor de vaso e planta para paisagismo. Conduziu-se este trabalho com o objetivo de estudar o enraizamento de estacas de três partes da planta: apical, mediana e basal; em três tipos de substratos: terra, areia e vermiculita, para a formação de mudas. As estacas foram retiradas das plantas matrizes e divididas em segmentos apical, mediano e basal, os quais foram colocados para enraizar em bandejas de isopor, nos substratos areia, terra e vermiculita, em estufa com nebulização. As estacas que apresentaram melhor qualidade de raízes foram as originadas da parte basal do ramo, sendo que o melhor enraizamento foi observado nas estacas colocadas em areia. As estacas obtidas de diferentes partes da planta não se diferenciaram quanto à porcentagem de enraizamento e quanto ao número de brotos formados. Já os substratos que proporcionaram o maior número de brotações foram a areia e a terra. Algumas estacas apresentaram formação de flores, ocorrendo com maior freqüência nas estacas apicais, sem efeito dos substratos. A areia foi o substrato que proporcionou os melhores resultados quanto à qualidade das raízes e porcentagem de enraizamento. O substrato terra foi superior apenas para o número de brotos por estaca, porém não diferenciou estatisticamente da areia. Mudas formadas de estaca da parte basal da planta se mostraram com qualidade superior.

Enraizamento de estacas de café cv. mundo novo submetidas a tratamentos auxínicos e com boro

The present research had aimed at studying the auxins and or boron effect on rooting of coffee (Coffea arabica L. cv. Mundo Novo') cuttings. The cuttings were obtained from semi-hardwood orthotropous branches of coffee-tree, containing 2 nodes and with aproximately 10 cm in length. The bases were dipped in treatment solutions composed of IBA or NAA with and without boron during 24 hours. After the treatments, the cuttings were planted in vermiculite. The following could be observed on cuttings taken ninety days after planting - total number of roots formed, average number of roots per cutting and root length. From the results obtained, it can be concluded that cutting treatment with NAA 100 ppm plus boron is responsible for the better rooting of coffee cuttings.

Desenvolvimento in vitro de copo-de-leite: efeito das concentrações de sacarose e de ácido giberélico

Calla lily (Zantedeschia aethiopica) is appreciated as cut flower and for the composition of gardens. However, many pathogens affect this species. By the traditional method of propagation, some units of new seedlings can only be produced annually. Tissue culture allows fast large-scale clonal propagation and provides healthy uniform plants. During the in vitro process, type and concentration of growth regulator could affect the growth of seedlings. Thus, the aim of this work was to determine sucrose and GA(3) concentrations to increase the efficiency of the in vitro multiplication of calla lily. After 60 days, the length of the above ground part and the roots, the number of sprouts, roots and leaves, above ground part and root fresh weight of seedlings were evaluated. The experimental design was entirely randomized with four replications. It was necessary the addition of 60.5 g L-1 sucrose associated to 5 mg L-1 GA(3) to obtain hight sprouts number. For higher length of the above ground part the addition of 45.3 g L-1 sucrose and 10 mg L-1 GA(3) was enough. Better results in the root length and number of roots were observed only in the sucrose presence, in concentrations in the range of 51.13 - 56.5 g L-1.

Avaliações bioquímicas séricas e hepáticas do extrato de yacon (Polymnia sonchifolia) Poepp. & Endl.

Yacon is a medicinal plant, the tuberous roots of which have been thought to contain a large amount of fructan (oligosaccharides). Purpose - The aim of paper was to study the effect of aqueous extracts of yacon on biochemical parameters of clinical importance in rats. The animals (male, wistar, weighing approximately 300g) were divided in 3 groups: G1(n=8)= water control; G2(n=8)= aqueous extract of roots (0,17g/100g/day); G3(n=8)= aqueous extract of leaves (25mg/100g/day). The serum samples were obtained after 30 days, and the biochemical parameters were measured. The livers were removed and homogenized in 0,01M phosphate buffer pH 7,0 and then the supernatant fractions were used for enzyme assay. Significantly increased serum glucose was observed in G2 (206,72±91,27 mg/dL). The groups G2 and G3 rats had higher (p<0,05) urea concentration, while creatinin level decreased (p<0,05). The serum albumin concentration showed a tendency to remain in G2 (2,44±0,45 g/dL) and G3 (2,84±0,50 g/dL). Aqueous extracts administration markedly decreased (p<0,05) the activities of ALT in the liver and greatly increased these enzymatic activities in blood. The serum alkaline phosphatase activity increased (p<0,05) in rats receiving the aqueous extract of root (192,75±20,95 U/dL), while aqueous extract of leaves reduced it (129,57±19,93 U/dL). The results indicated that the extract of yacon promoted changes of the biochemical parameters.

Efeito da sacarose e sorbitol na conservação in vitro de Passiflora giberti N. E. Brown

This work objectified the study of sucrose and sorbitol effect in the in vitro conservation for Passiflora giberti N. E. Brown, access. Therefore, an experiment was conducted in a completely randomized design to compare control treatment (standard MS) to MS medium supplemented with three sucrose concentrations (0, 15 and 30 g L -1) combined with three sorbitol concentrations (10, 20 and 40 g L -1), in a total of 10 treatments with 20 replicas. The experiment evaluation was carried out at 30, 60, 90 and 120 days of incubation, whereas the height of shoots (cm), number of roots, number and color of leaves were observed. The results showed the possibility to maintain passion-fruit microplants for a four months period under slow growth in MS medium supplemented with 10 or 20 g L -1 of sorbitol, without sucrose, and kept under 16 hours photoperiod (22 μ E m -2 s -1) and temperature of 27 ± 1°C. Sucrose sustained the longest development of the microplants. Root formation was affected by the sorbitol in the concentration of 40 g L -1 and by the absence of sucrose in the culture medium.

Sucessão sedimentar do Grupo Bauru na região de Pirapozinho (SP)

There is yet enthusiastic debate in the literature about the environmental conditions that originated the Cretaceous deposits of the Bauru Group, despite many authors accept that arid climatic conditions widely dominant at the base, evolved to chiefly fluvial-lacustrine conditions at the intermediate portion, and to arid conditions again at the top of the unit. The Bauru Group covers an area of about 117.000 km 2 of the Paraná Basin in São Paulo State territory. Core samples of this lithostratigraphic unit collected from a drill hole at Pirapozinho (Southwest of the São Paulo State) are described and together with well log data brought new information that do not agree with the described model. It was identified in this well the Caiuá, Pirapozinho, Santo Anastácio, Araçatuba and Adamantina formations. The study of these core samples clearly showed the dominance of hydrodynamic sedimentary structures and high to medium intensity of bioturbation in whole profile. These characteristics observed in core samples and compared to patterns of geophysical logs testify the dominance of fluvial processes in the Bauru Group deposition at the studied area. These new data suggests that the paleo-environmental evolution of the unit was much more complex, showing strong lateral and vertical changes that diverges from the model more widely accepted in the literature.

Alterações morfoanatômicas em plantas de Lithraea molleoides (Vell.) Engl. submetidas ao alagamento

Flooding effects in Lithraea molleoides plants were studied. Young plants were kept under drained and flooded soil over a period of 35 days. For growth and development analyses, the length and diameter of stem and main root and the dry weight of roots, stem, and leaves were measured. For anatomical studies, sections of fresh and fixed roots, stem bases and leaves were made using standard procedures in vegetal anatomy. The stress reduced the dry weight increment of plants without causing the death of roots or the abscission of leaves. In the stem base, flooding induced the hypertrophy of lenticels and the increase of intercellular space and reduction lower starch contents, in the cortex. Plants flooded displayed greater percentage of cortical intercellular space in the secondary roots and lower investment in secretory structure formation in the stem base. It can be suggested that flooding reduced the recourses allocation to growth. These recourses could be used in morphological alterations, such as hypertrophied lenticels and increase of intercellular spaces, that could contribute to plants survival during stress period, probably, due maintenance of aerobic respiration.

Espermidina e espermina exogena na micropropagação de Aloe vera (L.) Burm

The lateral shoots of the Aloe vera L. Burm have been inoculated in culture medium. The work has been divided in two phases. In the phase I different concentrations of NAA and BAP were added to the MS . In the phase II, plants proceeding of the MS were inoculated in medium containing or not spermidine and/or spermine. In the phase I the best results for mass production and shooting came out with the use of MS + 8,88 m mol L -1 BAP + 5,36 m mol L -1 NAA and these treatments didn't induce the rooting. Plants submitted to treatments with polyamines presented emission of roots during the phase II, suggesting possible inductor effect when used by isolated means during the rooting. No traces of oxidation were noted in the culture medium containing the polyamines. The use of spermidine in the culture-medium promoted a greater mass increasing and the most number of shoots was obtained with the use of the combination of polyamines. © 2008 1999, Editorial Ciencias Médicas.

Níveis constitutivos de compostos fenólicos podem estar relacionados à resistência da cana-de-açúcar à cigarrinha-das-raízes

The interaction of the leafhopper-of-roots with the sugar cane is still poorly marked and there is little information on resistant varieties. The phenolic compounds are involved in resistance mechanisms of the antibiosis type, but few studies are devoted to the studying role of these compounds in the interaction of plants with sucking insects. The study was conducted to determine how the sugar cane responded to the infestation of Mahanarva fimbriolata in terms of accumulation of phenolic compounds. An experiment was carried out under controlled conditions in a randomized design in a factorial schedule 3 x 2 x 4, with 3 genotypes and sugar cane, 2 levels of nymphs infestation of M. fimbriolata and sampling 4 times, with 4 repetitions. The genotypes SP80-1816 and RB72454 showed higher levels of total phenols when subjected to an infestation of the pest, but the duration of the nymphal stage and mortality of sharpshooters were significantly lower in those varieties, indicating that the increase in the concentration of phenolic compounds may had been caused by death and decay of the roots. It was not observed variation in levels of total phenols in the variety SP83- 5073 subject to an infestation of M. fimbriolata. However, the mortality of the leafhopperof- roots and duration of the stage of nymphs were significantly higher in genotype, indicating the existence of resistance-type antibiosis. Analysis of correlation showed that higher initial levels of phenolic compounds resulting in mortality of the pest can increase the duration of the nymph stage.

Crescimento de cultivares de mamoneira em função da aplicação de boro, durante a formação de mudas

It was objectified to quantify the initial growth of varieties of castor bean with boron fertilization. Had been tested to cultivate they AL-Guarani, Mirante 10 and Nordestina to the doses of 0; 0.25; 0.50; 0.75 and 1.0 mg kg -1 of B in controlled environment of greenhouse in the Sector of Cafeicultura of the Department of Agriculture of the UFLA. The characteristics valued were: height of plants, length of roots, leaf number, diameter of stem, mass of dry substance (MS) of leafs, of stem, the root and total. To cultivate Mirante 10 it was the one that got minor growth of the system of root. For leaf number to cultivate AL-Guarani it was superior to Mirante 10 and Nordestina. Also to cultivate better AL-Guarani if it detached how much to the production of total mass of MS, while to cultivate Mirante 10 produced more mass of MS than Nordestina.

Distribuição de cladóceros planctônicos (Crustacea: Branchiopoda) em um reservatório eutrófico raso (Paraná, Brasil)

This study focused the spatial and temporal distribution of the composition, abundance, and diversity of planktonic cladocerans from eutrophic, Iraí Reservoir, as well as their relationships with some biotic and abiotic variables. The tested hypothesis was that cladocerans present higher variation in a temporal than in a spatial scale. The samples were taken monthly in 6 stations, from March/02 to July/03. Twenty-four taxa were identified, distributed in 7 families, the richest families being Daphniidae (6 spp.), Chydoridae (6 spp.), and Bosminidae (5 spp.). The most frequent and abundant species were Bosmina hagmanni, Moina minuta, and Ceriodaphnia cornuta. The highest abundances were found in September/2002. Temporally, rainfall influenced organism's distribution, while spatially cladocerans were more affected by reservoir hydrodynamics and wind action. The low species richness could be a reflection of the trophic state of the reservoir, in which a dominance of Cyanobacteria was observed during that study period. Both scales showed high variation, but only the temporal scale showed significant difference to richness and abundance. Nearby the end of this study, higher stable values of species richness were recorded, which could suggest an increase in the water quality due to des-pollutions actions.

Soluções nutritivas no desenvolvimento do crisântemo cultivado em vaso

Although the information about nutritive solution for potted chrysanthemum is still incongruent, the objective of this work was to evaluate the effect of nutritive solutions in the development of Miramar chrysanthemum cultivated in pots at FCAV-UNESP, Jaboticabal-SP. The experiment had a randomized block design with split-plot evaluation and treatments corresponding to four nutritive solutions consisting of commercial fertilizers (S1, S2, S3, S4) evaluated at 0, 14, 28, 42, 56 and 70 days after rooting (DAE), with five replications. The plant height, leaf area, number of leaves, stem diameter, total dry matter mass of aerial part and roots; number and diameter of inflorescences, and physiological indexes of growth analysis were evaluated. The nutritive solutions did not interfere significantly in stem diameter, number and diameter of inflorescences and dry matter of roots, although S3 and S4 provided the highest plants, leaf area, number of leaves, total dry matter of aerial part. The physiological indexes showed that plants presented an accelerated growth from 14 to 28 DAE with S4 and S3 solutions and therefore they were considered the best solutions.

Dinâmica de cátions na raiz e folhas de berinjela cultivada sobre doses crescentes de potássio oriundas de duas fontes

This study was conducted at the Department of Plant Production, Sector Horticulture, UNESP - Botucatu, SP, Brazil, in order to assess the dynamics of cationic nutrients in roots and leaves and effects on dry matter production of roots and shoots in eggplant grown on conditions of increasing doses of potassium from two distinct sources (KCl and K 2SO 4). The experiment was arranged in randomized blocks in factorial 2 × 4 (two sources of potassium, KCl and K 2SO 4 and four doses of each source equivalent, 250, 500, 750 and 1000 kg ha -1 K 2O) comprising eight treatments with three replicates. For the experiment we used Oxisol medium texture (615 g of sand, silt 45 g and 340 g clay per kg soil). The characteristics evaluated were: soil electrical conductivity, mass of shoot and root material, content and ratio of K +, Ca 2+ and Mg 2+ in roots and leaves of eggplant. We conclude that excessive doses of sources of K 2O affected the production of dry matter of roots and shoots, as well as electrical conductivity. The accumulation and the relationship of Ca2+/K+, Mg 2+/K + were affected by increased K + concentration; K 2SO 4 was the fertilizer that was less damaging.