Distribution and habitat preference of Carabidae and Staphylinidae (Coleoptera) in an orange orchard and a forest fragment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Distribution of Xylella fastidiosa in citrus rootstocks and transmission of citrus variegated chlorosis between sweet orange plants through natural root grafts

To study translocation of Xylella fastidiosa to citrus rootstocks, budsticks from citrus variegated chlorosis (CVC)-affected cv. Pera sweet orange (Citrus sinenesis (L.) Osb.) were top grafted on 15 citrus rootstocks. Disease symptoms were conspicuous 3 months later on all 15 rootstocks tested. The presence of X. fastidiosa was confirmed by light microscopy, double-antibody sandwich enzyme-linked immunosorbent assays, and polymerase chain reaction in rootlets and main roots of CVC-symptomatic Pera sweet orange in 11 of the 15 rootstocks tested. These results suggest that bacterial translocation from the aerial plant parts to the root system occurs but is not essential for X. fastidiosa to induce symptoms in the aerial parts. Bacterial translocation to the roots was not correlated with CVC leaf-symptom severity in the Pera scion. To determine if CVC disease could be transmitted by natural root grafts, two matched seedlings of each of four sweet orange cultivars (Pera, Natal, Valencia, and Caipira) were transplanted into single pots. One seedling rootstock of each pair was inoculated by top grafting with a CVC-contaminated budstick while the other seedling rootstock was cut but not graft inoculated. Transmission of X. fastidiosa from an inoculated plant to a noninoculated plant sharing the same pot was observed in all four sweet orange cultivars tested. Transmission was confirmed by observation of natural roots grafts between the two plants, presence of X. fastidiosa in the root grafts, and disease development in the uninoculated plants. This is the first report of transmission of CVC disease through natural root grafts.

Susceptibility of tangerines to citrus variegated chlorosis

Citrus variegated chlorosis (CVC), a citrus disease first discovered in Brazil in 1987, is caused by the bacterium Xylella fastidiosa and transmitted by sharpshooters and budwood. Since the disease affects almost all sweet orange cultivars, it has become one of the most serious problems for Brazilian citriculture. To evaluate their resistance to CVC disease, fifteen tangerines or mandarins (C. reticulata Blanco) and their hybrids were grafted on Rangpur lime (C. limonia Osb.) and inoculated with CVC-contaminated Pera sweet orange (C. sinensis (L.) Osb.) by twig grafting in a greenhouse. Tangerines and their hybrids Wilking, Fortune, Sunki, Ellendale, Orlando tangelo, Nunes clementine, Nova, Sun Shu Sha Kat, Suenkat, and Batangas showed CVC leaf symptoms and gave positive results on enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) (with specific primers for X. fastidiosa), indicating that they are susceptible to CVC. Although X. fastidiosa bacteria were detected by ELISA and PCR in inoculated plants of tangerines Cravo and Oneco, no CVC leaf symptoms were observed on these two cultivars, suggesting that they are tolerant to the disease. CVC leaf symptoms were not observed and X. fastidiosa was not detected in tangerine Dancy and mandarins Okitsu satsuma and Ponkan after inoculation, showing that they are resistant to the disease.

A review of antifertility folkloric plants tested in laboratory animals

The use of natural active principals is widespread among a great proportion of the rural population, or by people who do not have easy access to medical assistance. These active principles are used as food or medicines, and even for purposes of contraception. It becomes necessary to establish a relationship between the folklore habits and current information on the nature of anti-fertility substances, and knowledge of their mechanisms. Anti-fertility agents may exert their actions in a number of areas, (hypothalamus, anterior pituitary, oviduct, uterus, and vagina), inhibiting synthesis and/or liberation of hormones (follicle-stimulating, luteinizing, and steroid hormones), ovulation, ovum transportation, and implantation process. Therefore, a review of literature was carried out, including of several plants used by women as abortifacient and anti-fertility agents to compare their effects with those obtained among laboratory animals.

Presence of Xylella fastidiosa in sweet orange fruit and seeds and its transmission to seedlings

Xylella fastidiosa, a xylem-limited bacterium, causes several economically important diseases in North, Central, and South America. These diseases are transmitted by sharpshooter insects, contaminated budwood, and natural root-grafts. X. fastidiosa extensively colonizes the xylem vessels of susceptible plants. Citrus fruit have a well-developed vascular system, which is continuous with the vascular system of the plant. Citrus seeds develop very prominent vascular bundles, which are attached through ovular and seed bundles to the xylem system of the fruit. Sweet orange (Citrus sinensis) fruit of cvs. Pera, Natal, and Valencia with characteristic symptoms of citrus variegated chlorosis disease were collected for analysis. X. fastidiosa was detected by polymerase chain reaction (PCR) in all main fruit vascular bundles, as well as in the seed and in dissected seed parts. No visual abnormalities were observed in seeds infected with the bacterium. However, the embryos of the infected seeds weighed 25% less than those of healthy seeds, and their germination rate was lower than uninfected seeds. There were about 2,500 cells of X. fastidiosa per infected seed of sweet orange, as quantified using real-time PCR techniques. The identification of X. fastidiosa in the infected seeds was confirmed by cloning and sequencing the specific amplification product, obtained by standard PCR with specific primers. X. fastidiosa was also detected in and recovered from seedlings by isolation in vitro. Our results show that X. fastidiosa can infect and colonize fruit tissues including the seed. We also have shown that X. fastidiosa can be transmitted from seeds to seedlings of sweet orange. To our knowledge, this is the first report of the presence of X. fastidiosa in seeds and its transmission to seedlings.

Eficiência de extratos de plantas medicinais e aromáticas em atrair a abelha africanizada (Apis mellifera L.) em cultura de abacateiro (Persea americana Mili.)

The objective of the experiment was to test attractive substances for Africanized honey bees, Apis mellifera L., in avocado, Persea americana Mill. Were pulverized extracts of lemon grass (Cympobogon citratus), ocimum (Ocimum sellowii), Lippia alba, orange leaves (Citrus sinensis), eucaliptus leaves (Eucalyptus sp), eugenol and linalol and the frequency of bees on flowers were evaluated. The frequency of honey bees did increase with the application of the extracts falsa melissa, eucaliptus leaves and orange leaves, used as attractants in Quintal variety. The products tested in Eppendorf tubes did not increased the frequency the bees in Quintal and Fortuna varieties.

Tabela de vida ecológica do minador-dos-citros, Phyllocnistis citrella stainton (Lepidoptera: Gracillariidae)

The construction of ecological life tables is a useful tool to understand the population dynamics of insects in the field. In this work, ecological life tables were developed for Phyllocnistis citrella (Stainton) in an orchard of Citrus sinensis situated in Santa Rita do Passa Quatro, Sao Paulo State, during 2002. The occurrence of generation overlay in the field was avoided by obtaining the eggs from infestation on young citrus plants (Citrus limonia) kept under controlled conditions. The samples were taken every other day and the number of live, dead, predated and parasitized individuals were recorded. The occurrence of arthropod predators was determined through visual search on the plants of the orchard. The key factors of mortality acting on P.citrella populations were: pupae parasitism caused by Ageniaspis citricola (Logvinovskaya), action of uncertain factors on prepupae and predation of 3rd-instar larvae. The most abundant arthropod predators belonged to the families Formicidae, Chrysopidae, Coccinellidae, Reduviidae e Araneae. In two of five generations of P. citrella, approximately 4% of A. citricola pupae were hyperparasitized by Galeopsomyia fausta (LaSalle) (Hymenoptera: Eulophidae).

Avaliação dos teores nutricionais em laranjeira 'Valência', sob fertirrigação

This work aimed to compare fertigation to conventional fertilization in a five-year-old 'Valência' orange orchard, irrigated by microsprinklers. The conventional fertilization, using the recommended dosage for the crop, was parceled out in three during the year. In the fertigation treatments a complete dose, 1/2 and 1/3 of the conventional fertilization dose, parceled out in ten in a year, were used. Trunk circumference and leaf volume, leaf chlorophyll content and fruit yield and quality were measured. For the first year, significant differences among the treatments for the evaluated parameters were not observed; however, it was noticed that fertigation treatments caused less nutritional unbalance, obtained by DRIS. The fertigation treatment using half dosage showed the best result regarding to fruit yield and quality.

Consumo e custo de energia elétrica na cultura de citros irrigado por gotejamento e microaspersão, com três lâminas de água

The objective of this work was to analyze the consumption, electric energy cost, and economic results of irrigated citrus (Citrus sinensis). The treatments consisted of a dripping irrigation system with one and two lateral distribution lines, a micro sprinkler irrigation system and a treatment without irrigation. For each irrigation system, three water depths were used: 100%, 75% and 50% of Etc (citrus evapotranspiration). The electric energy cost for two tariff groups, Group A and Group B, was studied. For Group A, the expenses with energy were determined for the Conventional Binomial Structure tariff, the Hour-seasonal tariff (green and blue) and the special tariff for nocturnal irrigation. The kWh cost for the tariff systems were obtained from the website of CPFL (São Paulo State Power and Light Company, Brazil). The best relation between the electric energy consumption (kWh.ha -1) and productivity (t.ha -1) occurred in the treatment irrigated with 50% of the Etc. The irrigated treatments increased productivity. The biggest productivity was observed in the irrigation treatments with 50% of the Etc when compared to the ones with 100% of the Etc. The blue and green Hour-seasonal tariff system of Group A (nocturnal irrigation) was the best option. A biggest economic turnover occurred in the treatments irrigated with 50% of the Etc.

Effect of an acid diet allied to sonic toothbrushing on root dentin permeability: An in vitro study

This study quantified alterations in root dentin permeability after exposure to different acid beverages. Twenty-five third molars were sectioned below the cementoenamel junction, the root segment was collected, and the pulp tissue was removed. The root segments were connected to a hydraulic pressure apparatus to measure the permeability of root dentin after the following sequential steps, with 5 specimens in each: 1) phosphoric acid etching for 30 s (maximum permeability), 2) root planning to create new smear layer, 3) exposure to different acid substances for 5 min (orange, cola drink, vinegar, white wine, lemon juice), 4) toothbrushing with sonic toothbrush for 3 min, 5) toothbrushing with sonic toothbrush plus dentifrice for 3 min. Considering step I as 100%, the data were converted into percentage and each specimen was its own control. Data were analyzed statistically by Kruskal-Wallis and Dunn's post test at 5% significance level. All acidic substances increased dentin permeability significantly after scraping (p<0.05). Toothbrushing after exposure to acid substances decreased dentin permeability and the association with dentifrice accentuated the decrease (p<0.05), except for the specimens treated with cola drink. Thus, it may be concluded that all tested acid fruit juices increased dentin permeability, and toothbrushing with or without dentifrice can decrease root dentin permeability after dentin exposure to acid diet.

Geoinformação na gestão dos recursos hídricos em sub-bacia hidrográfica

By remote sensing, geodatabase digital processing, information and expeditions to Capivara's sub-basin, was possible to identify the changes in the landscape mainly the expansion of eucalyptus, sugar-cane, and orange trees, being the last two, mainly on the Periferic Depression of Basaltic Cuesta. A significant portion of the soil on this geological area is formed from sandstones, providing high permeability to them, making them important places to groundwater recharge areas as sensible to contamination by pesticides. Throughout last decade was observed that the native vegetation fragments stabilization, keeping a reason of 26.5% on the land used between 2000 and 2010. The pasture decrease being substituted by eucalyptus, sugar-cane and orange trees call attention for the changes in the agribusiness model demanded by the current economic and social necessity. Pasture decrease followed by erosions decrease on sub-basin is evidence that these two aspects are strongly related.

Daños causados por cancro cítrico en la producción de naranja dulce 'Valencia'

The citrus canker, caused by Xanthomonas axonopodis pv. citri, is one of the main citrus diseases and has threatened the world's citriculture. In this study, the damage caused by citrus canker was quantified in a sweet orange culture, cv. Valencia [Citrus sinensis (L.) Osb.], grafted over 15 year-old Rangpur lime (C. limonia Osb.), in the municipality of Guatambu, SC, Brazil, in the crop of 2004/05. Evaluations of the number of fruits per plant (NF), fruit yield per plant (P), citrus canker incidence on the fruit (IFR), and incidence (IL) and severity (SV) of citrus canker on 80 leaves per orange tree were performed in the harvest period, applying diagrammatical scales. Plants had large variation in SV (0.16%-1.09%), IL (12.50%-56.25%) and IFR (12.30%-56.09%). Linear regressions were significant between IL x SV, NF x SV, P x IFR, and NF x IFR. There was no significant difference (p>0.05) between the mean estimated values of IL in different quadrants of the crowns of the orange trees (N, S, L and O). Each 1% increase in IFR reduced 2.16 kg and 21.3 fruits per tree.

Posicionamento de Extratores de Cápsula Porosa em Solo Arenoso Na Citricultura Fertirrigada Por Gotejamento

The efficiency of monitoring fertigation by means of porous capsule extractors is directly related to its correct placement, since the nutrients applied through fertigation have different mobility throughout the soil profile. The objective of this study was to evaluate the distributions of NO3, P, and K in the soil solution, when applied in citrus in sandy soil through drip fertigation systems, aiming to define the proper placement of the solution extractors. In order to measure the concentration of ions in the soil solution, the extractors were installed at four different distances (5, 15, 25, and 35 cm) and depths (15, 30, 60, and 90 cm) with an emitter located under the projection of the tree canopy. The experiment was conducted in a Valencia orange orchard on citrumelo Swingle rootstock, in Reginópolis/SP. The experimental design was randomized blocks and the treatment arrangement was a 4 x 4 factorial design with five repetitions. Sixteen soil solution extractors were installed per block, with a total of 80 extractors in the experiment. According to the obtained results, to determine P and K, it is recommended to install the solution extractor at 15 cm horizontal and 30 cm depth from the emitter. For NO3, the extractor recommended placement is 25 cm horizontal and 30 cm depth. The soil solution extractor proved to be a sensitive tool, capable of determining the ions mobility in the wet bulb.

Consumer interpretation of ready to drink orange juice and nectar labelling

The aim of this study was to evaluate consumers' use and interpretation of ready to drink orange juice and nectar label information and its influence on the purchase decision. One hundred and sixty-seven consumers of ready to drink orange juice and nectar were interviewed. The labels were analysed to evaluate their conformance to Brazilian legislation. The manufacturing and shelf life date were the information most often checked, followed by health related issues. Brand, price and flavour were the most important factors for purchase decision. Brand and flavour showed significant association with consumer age. For most interviewed, 'nectar', 'whole' and 'natural' or '100% natural' were not well understood; they were not in accordance with the Brazilian legislation. 'Nectar', 'whole' and 'natural' or '100% natural' received a positive interpretation, whereas 'reconstituted juice' was considered a negative expression. Nevertheless, none of the labels completely conformed to the specific nutritional labelling legislation. © 2013 Institute of Food Science and Technology.

Aspectos técnicos e econômicos da poda e do controle químico de Brevipalpus phoenicis no manejo da leprose dos citros

The objective of this study was to evaluate different citrus leprosis management tactics during seven seasons, based in pruning and acaricide applications, considering technical and economic aspects of each tactic. The trial was conducted from October 2003 to August 2010 in an orange plantation of Pera cv. located in the municipality of Reginópolis-SP, Brazil. The plants of citrus used were12 years old and grafted onto Cleopatra tangerine. The experimental design used was randomized blocks, in a factorial scheme, made up of factors type of pruning (A), with six levels: (1) drastic pruning, (2) intermediate pruning without leprosis lesions, (3) intermediate pruning with leprosis lesions (4) light pruning, (5) without pruning and (6) replant; acaricide applications factor (B), with three levels: (1) without acaricides applications, (2) with lime sulfur applications and (3) spirodiclofen or cyhexatin applied in rotation; pruning factor to remove leprosis symptomatic branches (C), with two levels: (1) with pruning for removal, (2) without removal pruning. The combination of factors, with respective levels (6 x 3 x 2), resulted in 36 treatments that were repeated four times, with each parcel being made up of three plants in a row. After seven years, it was observed that the types of the pruning and remove of leprosis symptomatic branches used as single management tactic, is not sufficient to leprosis control. Therefore, the results demonstrated that for leprosis management, it is essential the association between tactics, especially the control mite vector. For ensuring the citrus production economically, the use of acaricides highly efficient is essential in B. phoenicis control. The recommendation of the type of pruning should be in function of the leprosis incidence and severity in the orchard. In orchards with low leprosis incidence and severity the light pruning is more appropriate, because it is efficient and ensures greater financial balance. However, in orchards with high leprosis incidence and severity it is indicated the severe pruning for reducing or eliminating inoculum source. Between the severe prunings, the intermediate pruning with leprosis lesions is the most relevant, because the financial return will be faster. Replant is indicated only for young orchards, because does not imply in changes onto cultural practices.