Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of fluoride varnish supplemented with sodium trimetaphosphate on enamel erosion and abrasion: An in situ/ex vivo study

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

In vitro Remineralizing Effect of Fluoride Varnishes Containing Sodium Trimetaphosphate

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Inhibition of tooth erosion by milk containing different fluoride concentrations: An in vitro study

Objectives: This in vitro study assessed the effect of milk containing different fluoride concentrations on tooth erosion.Methods: Bovine enamel and root dentine specimens were treated with: (1) bovine whole milk with 0 ppmF; (2) 2.5 ppm F; (3) 5 ppmF;(4) 10 ppmF (all after erosion); (5) whole milk with 0 ppm F (before erosion); (6) NaF (0.05% F, positive control, after erosion) or (7) 0.9% NaCl (negative control, after erosion). The specimens were submitted to pH cycles (4 x 90 s in soft drink) and treatments for 5 days. The specimens were immersed in the treatment solutions for 1 min(only at the first cycle each day) with further exposition to 1: 1 milk: saliva slurry for 10 min. The tooth loss was measured using a contact profilometer and statistically analysed (p < 0.05).Results: Rinsing with milk before erosive challenge significantly reduced tooth loss compared to negative control (67% and 24% reduction in dentine and enamel loss, respectively) and to milk after erosive challenge, only for dentine. The addition of fluoride to milk also reduced tooth loss compared to negative control, but with no significant differences among fluoride concentrations for enamel and dentine (mu m), respectively: 0 ppm (3.63 +/- 0.04 and 2.51 +/- 0.53), 2.5 ppm F (2.86 +/- 0.42 and 1.96 +/- 0.47), 5 ppm F (2.81 +/- 0.27 and 1.77 +/- 0.44), 10 ppm F (2.03 +/- 0.49 and 1.68 +/- 0.59). There was a negative and significant correlation between [F] and the tooth loss.Conclusions: Daily rinse with milk containing F is able to reduce both enamel and dentine erosion in vitro.Clinical significance: Since the prevalence of dental erosion is steadily increasing, rinse with milk or its derivate might be an important strategy to reduce the progression of tooth erosion. (C) 2013 Elsevier Ltd. All rights reserved.

Plaque fluoride concentrations associated to the use of conventional and low-fluoride dentifrices

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of fluoride gels supplemented with sodium trimetaphosphate in reducing demineralization

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of low-fluoride toothpastes combined with hexametaphosphate on in vitro enamel demineralization

Objectives: The aim of this study was to evaluate the anticaries effect of low-fluoride toothpastes combined with hexametaphosphate (HMP) on enamel demineralization.Methods: Bovine enamel blocks were subjected to pH cycling and treatment with toothpaste's slurries (15 groups; 2x/day). Toothpaste mixtures contained the following: no fluoride (F) plus HMP (from 0 to 3.0%); 250 ppm F plus HMP (from 0 to 3.0%); 500 ppm F; 1100 ppm F; and a commercial toothpaste (1100 ppm F). After pH cycling, surface and cross-sectional hardness, as well as F present in the enamel were determined. The demineralization depth was analyzed using polarized light microscopy. The variables were subjected to 1-way ANOVA, followed by Student-Newman-Keuls' test (p < 0.05).Results: In the absence of fluoride, 0.5% HMP promoted the lowest mineral loss and its effect was similar to that of a 250 ppm F toothpaste (p > 0.05). The combination of 0.5% HMP and 250 ppm F resulted in lower mineral loss (p < 0.05) and similar lesion depth when compared to the 1100 ppm F toothpaste (p > 0.05).Conclusion: To conclude, the combination of 0.5% HMP and 250 ppm fluoride in a toothpaste has a similar inhibitory effect on enamel demineralization in vitro when compared to a toothpaste containing 1100 ppm F.Clinical significance: The anticaries effect of toothpaste containing 250 ppm F combined with 0.5% HMP was similar to that of a 1100 ppm F toothpaste, despite the 4-fold difference in F concentration. Although such effects still need to be demonstrated in clinical studies, it may be a viable alternative for preschool children. (C) 2013 Elsevier Ltd. All rights reserved.

The effect of chronic treatment with fluoride on salivary activity, tooth, and bone in spontaneously hypertensive rats (SHR)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The effects of low-fluoride toothpaste supplemented with calcium glycerophosphate on enamel demineralization

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effect of fluoride varnish supplemented with sodium trimetaphosphate on enamel erosion and abrasion

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Streptococcus Mutans Adhesion to Titanium After Brushing with Fluoride and Fluoride-Free Toothpaste Simulating 10 Years of Use

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Erosion Protection by Calcium Lactate/Sodium Fluoride Rinses under Different Salivary Flows in vitro

This study investigated the effect of a calcium lactate pre-rinse on sodium fluoride protection in an in vitro erosion-remineralization model simulating two different salivary flow rates. Enamel and dentin specimens were randomly assigned to 6 groups (n = 8), according to the combination between rinse treatments - deionized water (DIW), 12 mm NaF (NaF) or 150 mm calcium lactate followed by NaF (CaL + NaF) and unstimulated salivary flow rates - 0.5 or 0.05 ml/min simulating normal and low salivary flow rates, respectively. The specimens were placed into custom-made devices, creating a sealed chamber on the specimen surface connected to a peristaltic pump. Citric acid was injected into the chamber for 2 min, followed by artificial saliva (0.5 or 0.05 ml/min) for 60 min. This cycle was repeated 4x/day for 3 days. Rinse treatments were performed daily 30 min after the 1st and 4th erosive challenges, for 1 min each time. Surface loss was determined by optical profilometry. KOH-soluble fluoride and structurally bound fluoride were determined in specimens at the end of the experiment. Data were analyzed by 2-way ANOVA and Tukey tests (alpha = 0.05). NaF and CaL + NaF exhibited significantly lower enamel and dentin loss than DIW, with no difference between them for normal flow conditions. The low salivary flow rate increased enamel and dentin loss, except for CaL + NaF, which presented overall higher KOH-soluble and structurally bound fluoride levels. The results suggest that the NaF rinse was able to reduce erosion progression. Although the CaL prerinse considerably increased F availability, it enhanced NaF protection against dentin erosion only under hyposalivatory conditions. (C) 2014 S. Karger AG, Basel

In situ evaluation of low-fluoride toothpastes associated to calcium glycerophosphate on enamel remineralization

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Flammability limits of hydrated and anhydrous ethanol at reduced pressures in aeronautical applications

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Zinc blende versus wurtzite ZnS nanoparticles: control of the phase and optical properties by tetrabutylammonium hydroxide

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)