Atividade in vivo de parasporina extraída de B. thuringiensis sobre o câncer colorretal murino (CT‐26)

Bacillus thuringiensis (Bt), is an environmental Gram-positive spore-forming bacterium that produces crystalline parasporal protein (Cry) during sporulation. The inclusions often exhibit strong and specific insecticidal activity, making Bt an agent for agricultural controlling insects pest, mites, protozoa and nematodes. Recent studies reported that some of these Crys do not show cytotoxicity against insects but they are capable to kill some human and animal cancer cells. These proteins were denominated parasporins (PS). However, antitumor activity of Bt parasporin on the development of murine colorectal cancer (CT-26), are not well studies and these are no reports on the in vivo effect of these proteins. Thus, the present study evaluated the in vitro and in vivo anti-tumoral activity of Bt parasporin against the murine colorectal cancer line CT-26. Therefore, Balb/c mice were s.c. inoculated with CT-26 cells and weekly treated with parasporin (i.p.) pre-activated by enzymatic digestion with trypsin or proteinase K. Our results have shown, for the first time, that despite the anti-tumor activity in vitro, parasporin crystals couldn’t combat tumor growth in vivo. Instead, this protein was highly toxic, affecting the liver and spleen, with possible effect on other organs, decreasing the survival of treated animals. The results indicate the need for studies to better detoxification or manipulation of parasporin for therapeutic use and new studies for analysis of toxicological effects of repetitive exposure of farmers to this toxin

Efeito Pesticida de Ésteres de Sacarose Sobre Calacarus heveae Feres (Acari: Eriophyidae) em Dois Clones de Seringueira

Sucrose esters are biodegradable, non pollutant and safe for health; moreover, they have shown great potential in the pest control. We tested a crude mix of sucrose esters on the survivorship and oviposition of Calacarus heveae Feres (Acari: Eriophyidae) females, an important rubber tree pest mite. The females were collected from rubber leaflets of the GT 1 and RRIM 600 clones. The mites were kept on stock arenas placed in rearing chamber at 28 + 0,1°C, 80 + 10% of relative humidity and 12h of light phase about two weeks before the assays. After this period, the mites were transferred to assay arenas made with leaflets from the same clone of stock arenas. The survivorship of females sprayed with sucrose esters in the concentrations of 1, 2, 3 and 4 g/L and their oviposition effect with 0.5 and 1 g/L were analyzed. The mites sprayed with 4 g/L had about 80% of mortality, while those with 1 g/L around 60%. All mites that died in the treatments with sucrose ester became dark with wrinkled tegument and decreased their body volume, suggesting dehydration. We also observed the efficacy of 1 g/L concentration to decrease the female oviposition in about 50%, in the third day after spraying. No differences were observed in the mortality and oviposition between females kept on both clones.

Distribuição espacial e determinação da depreciação quantitativa e qualitativa causada por Tetranychus ogmophallos (Acari: Tetranychidae) ao amendoinzeiro

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Distribuição espacial e temporal de Brevipalpus phoenicis (Acari: Tenuipalpidae) virulíferos e da leprose-dos-citros em pomares em controle químico

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Imagem e epifania nos poemas em prosa de Cruz e Sousa

Partindo da conceituação de termos caros à teoria e à crítica da poesia lírica, perfaço um breve estudo da poesia em prosa do simbolista brasileiro Cruz e Sousa (1861-1898), consubstanciada nos livros Missal (1893) e Evocações (1898). Procurei evidenciar o processo de composição das duas obras e as diferenças entre ambas, frisando inclusive o modo pelo qual o poeta configura suas imagens poéticas. Assim, articulando os conceitos de Imagem e Epifania – utilizados pelo poeta de maneira moderna e original –, busquei ressaltar sua importância no processo construtivo de Cruz e Sousa, salientando, por fim, o lugar de destaque que o poeta ocupa nos quadros da poesia brasileira.

A identidade da literatura marginal em enunciados verbo-visuais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identidade e reconhecimento na cultura de rua

Direct descendant of the music of the Black Atlantic, hip hop became the spokesman of excluded minorities, for example setting up of the reunification that is geographically separated through communication. The hip hop appropriated new forms of mass communication, allowing the reworking of the story through a counter-hegemonic discourse that seeks self-knowledge and appreciation of the roots of black people. The street culture is the narrative of disenfranchised youth, generating narrative of inclusion, which gave a space of enunciation periphery enabling them to intersubjective recognition. This project aims to discuss the role of hip hop as an agent conscientizing youth peripherals in order to bring to the debate the form of identity construction around the peripheral street culture since, as an intervention that acts as a symbolic system guiding cultural practices and attitudes of these young people. Methodologically, we analyzed specific bibliographical about hip hop and literature originating from the periphery itself. Selected discography of some rap groups. They are Racionais Mc's, 509-E and DJ Hum and Thaíde that constitute the vanguard of hip hop in Brazil. We use music from rapper Emicida this while some contemporary exponents of rap, with national and international. State of São Paulo in order to demonstrate that there is similarity between the questions proposed by the hip hop and the interior of the capital, the group selected Survey. We have used also press material, raising the issue in research on the internet, periodicals include the Rolling Stones and Caros Amigos

Fauna de dípteros de morcegos (Chiroptera, Phyllostomidae): composição, taxa de infestação e prevalência em cinco áreas no estado de São Paulo

As taxas de infestação de dípteros ectoparasitos de Chiropteros foram estudadas em cinco regiões do Estado de São Paulo, os aspectos ecológicos da relação hospedeiro/parasitas em Chiroptera são pouco explorados em relação aos ectoparasitos e carecem de mais informações. Tendo tais aspectos em vista os objetivos desse trabalho foram: (1) listar a composição da fauna de dípteros ectoparasitos associados aos morcegos filostomídeos; e (2) examinar se houve diferenças nos valores de prevalência e incidência nas diferentes áreas de estudo (relacionando tais fatores também ao sexo e idade do hospedeiro).. No presente estudo foram coletados 378 indivíduos de dez espécies de dípteros ectoparasitas, sendo setes delas pertencentes a família Streblidae, uma a família Nycteribiidae, e ainda duas espécies de ácaros pertencentes a família dos Spinturnicidae. As espécies de ectoparasitas mais abundantes foram Pararichobius longincrus (21,9%), Trichobius joblingi (17,4%) e Megistopoda proxima (33,7%). As análises estáticas parasitológicas foram feitas as espécies mais abundantes, calculou-se o valor da prevalência, taxa média de infestação, e os diferentes valores relativos ao status reprodutivo, sexo e idade do hospedeiro., as taxas de prevalência encontradas no presente estudo foram similares a estudos anteriores feitos na região de São Paulo e no geral houve uma tendência as taxas de infestação e prevalência serem maiores em jovens e fêmeas grávidas e lactantes. Apesar de tal constatação não é possível afirmar que haja uma correlação estatística entre hospediros mais jovens e fêmeas grávidas e maiores taxas de prevalência. O estudo ajuda a elucidar a composição faunística dos ectoparasitos de Chiropteros, e a esclarecer alguns aspectos da relação ecológica entre parasita hospedeiro

As construções verbais paratáticas: gramaticalização em italiano

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do silicato de potássio em plantas de mamoeiro sobre a infestação do ácaro-rajado, tetranyhcus urticae koch

Pós-graduação em Agronomia - FEIS

Dental care for cerebral palsy patients using auxiliary resources to inhibit pathological tonic reflexes

Cerebral paralysis is a chronic illness that affects the central nervous system. In this article the author describes the techniques used at CAOE (Odontological Center for Assistance to patients with special needs) to restraint CP patients during dental treatment, because they present some pathological reflexes which interfere in their odontological assistance. Also it shows how to perform a special physical restraint and how to keep the mouth open by using simple tools including a homemade one. All the devices used during the dental treatment in cerebral palsy patients, such as the physical restraint with bands or sheet to wrap them up, cylindrical pad or cushion made of a soft material, or simple tools including a homemade one to keep the mouth open are usually and safely used in CAOE. All these simple devices are necessary, because there are no available funds for the acquisition of expensive material or equipment. Despite of a shortage of resources we can easily and efficiently assist these patients.

A pesquisa em televisão digital no Brasil: apontamento sobre o levantamento bibliográfico realizado de 1995-2008 sobre o assunto

In Brazil, the discussion about thereplacement of analog television signals to digitaland its impact on society, in media companies andgovernment began in the 90s. Embryonic steps towardsdigitalisation of the television signal ground were thegovernment of Fernando Collor de Mello (1990-1992),with advances during the government of FernandoHenrique Cardoso (1994-2001) and in the governmentof Luiz Inacio Lula da Silva (2002). The academy startsthe discussion in 1996. This article aims to bring to lightthe results obtained from the survey Digital television:a historical perspective , which received the supportof CNPq for funding of two scholarships for scientifi cinitiation between August 2008 and July 2009. Theproject sought to identify what has been published onthe subject in the fi eld of Social Sciences and from threemacrodescritores (information management, publicpolicy and value chain), providing the li ing of a virtualcollection Zotero bibliographic innovative platform.The mapping serves to show the growing interest anddevelopment of research in the country during theperiod - when the number jumped to three works inthe fi rst seven years (1995-2001) to 198 in the last sevenyears (2002-2008). The research sought to identify themajor authors and works related to digital televisionand published in the country. The survey took intoaccount books, articles published in books, journals,Annals of Compós and Intercom, and dissertations andtheses. Data and refl ections on the survey are containedin this communication.

Biologia e tabela de vida de Brevipalpus yothersi (Acari: Tenuipalpidae) oriundos de diferentes regiões citrícolas do Estado de São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Revisão taxonômica e análise filogenética das espécies de Daidalotarsonemus De Leon e Excelsotarsonemus Ochoa & Naskrecki (Acari: Tarsonemidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)