Estudo da organização e mapeamento cromossômico do elemento transponível Rex1 em espécies do gênero Astyanax Baird & Girard, 1854 (Characiformes: Characidae) provenientes de diferentes bacias hidrográficas

O gênero Astyanax apresenta uma extensa variabilidade em suas fórmulas cariotípicas, as quais acompanham sua diversidade biológica. Estes dados fazem com que a taxonomia do gênero ainda seja confusa, devido à grande plasticidade fenotípica existente entre as populações e a ausência de caracteres morfológicos confiáveis para sua correta identificação. Por outro lado, a análise das sequências e distribuição cromossômica do retrotransposon Rex1 revelou pouca variabilidade. As diferenças encontradas com relação às sequências deste retrotransposon mostram que as variações podem estar associadas à distribuição geográfica das espécies, visto que as espécies mais distantes geograficamente revelaram maiores diferenças em sua composição nucleotídica. A distribuição cromossômica desse elemento mostrou-se conservada entre as espécies do gênero, com marcações dispersas pelos genomas dos mesmos, contribuindo com a ideia de que esses elementos podem se acumular em regiões genômicas específicas dentro de cada grupo de peixes, sendo que esta tendência é maior para peixes do mesmo grupo e menor para grupos diferentes. Deste modo, a ampla distribuição de Rex1 observada nas espécies estudadas sugere que esse elemento pode ter sido incorporado há muito tempo nos genomas desses peixes, e que vem desempenhando um papel importante na evolução do grupo. Ainda assim, devido à grande diversidade encontrada no gênero, mais estudos podem contribuir tanto para o melhor entendimento da taxonomia de Astyanax, como da dinâmica evolutiva dos retrotransposons no gênero e em outros grupos de peixes

Modelagem de motor de combustão internae simulação do processo de queima de combustível

The internal combustion engine is a heat engine widely used in the automotive industry. In order to better understand its behavior many models in the literature have been proposed in the last years. The 0-D thermodynamic model is a fairly simple tool but it is very useful to understand the phenomenon of combustion inside the chamber of internal combustion engines. In the first phase of this work, an extensive literature review was made in order to get information about this kind of analysis and, after this, apply them in a model able to calculate the instantaneous temperature and pressure in one zone of the combustion chamber of a diesel engine. Therefore some considerations were made with the aim of increasing the accuracy of the model in predicting the correct behavior of the engine, adding the combined effects of heat transfer, leakage and injection. In the second phase, the goal was to study the internal flow of a three-dimensional model of an internal combustion engine. In order to achieve this goal the software Solidworks was used to create the geometries of an engine and the suite of softwares Ansys was used to create the moving mesh (ICEM CFD and CFX-Pre) and to solve the CFD problem (Ansys CFX code). The model was able to perform the air flow simulation during the four-stroke cycle of an engine: admission, compression, expansion and exhaust. The results obtained from both models were suitable and they open a new range of possibilities for future researches on the field

Predação de sementes do palmito juçara Euterpe edulis Martius em fragmentos florestais - testando o modelo Janzen-Connell

A extensa fragmentação das florestas tropicais e conseguinte perda da fauna de dispersores e predadores de sementes trazem importantes consequências às interações planta-animal. A predação de sementes, que representa uma etapa crítica no recrutamento de plantas tropicais, é um dos processos que sofrem com este impacto. A falta dos agentes dispersores e predadores de sementes pode alterar a dinâmica natural dessa interação trófica ao ponto de não corresponder mais ao modelo de Janzen-Connell, que prediz que quanto mais próximo da planta parental, maior a probabilidade de predação e, por conseqüência, menor será a dispersão. Esse estudo avaliou em três fragmentos com níveis diferenciado de defaunação (Mata São José, Reserva Municipal Santa Genebra e Estação Ecológica dos Caetetus) a predação (e/ou dispersão) de sementes de palmito juçara (Euterpe edulis Martius) abaixo e longe da planta-mãe, buscando testar o modelo Janzen-Connell. Em cada área cinco sementes foram dispostas em tratamento aberto e cinco em tratamento fechado, abaixo e distante 10 metros do parental em 15 palmeiras e avaliado o destino das sementes após 30 dias. Os resultados não oferecem suporte ao modelo, uma vez que apenas em um dos fragmentos pequenos e defaunados a predação abaixo do parental foi maior que distante, ou seja, nos demais fragmentos a distância da planta mãe não aumentou a sobrevivência das sementes. Nas áreas defaunadas a predação ocorreu principalmente por invertebrados e patógenos, enquanto que na área menos defaunada a predação foi feita principalmente por vertebrados. Diferenças significativas na predação de sementes entre os tratamentos só foram obtidas na área menos defaunada, o que mostra a importância dos predadores vertebrados de médio e grande porte na predação e/ou dispersão de sementes de E. edulis neste fragmento.

Produção de partículas secundárias em reações de “spallation” induzidas por prótons

Cosmic radiation has been identi ed as one of the main hazard to crew, aircraft and sensitive equipments involved in long-term missions and even high-altitude commercial ights. Generally, shields are used in spatial units to avoid excessive exposure, by holding the incident radiation. Unfortunatelly, shielding in space is problematic, especially when high-energy cosmic particles are considered, due to the production of large number of secondary particles, mainly neutrons, protons and alpha particles, caused by spallation reactions and quasi-elastic processes of the corpuscular radiation with the shield. Good parameters for checking the secondary particle production at target material are diferential cross section and energy deposited in the shield. Addition experiments, some computer codes based on Monte Carlo method show themselves a suitable tool to calculate shield parameters, due to have evaluated nuclear data libraries implemented on the algorithm. In view of this, the aim of this work is determining the parameters evaluated in shielding materials, by using MCNPX code, who shows good agreement with experimental data from literature. Among the materials, Aluminium had lower emission and production of secondary particles

Tomada de decisão em grupo na seleção de processos para auditoria interna

L’audit, c’est un outil de gestion très important pour que les organisations puissent accomplir la réussite. Selon le fait qu’il n’y a pas des modèles d’aide à l’audit, il existe une opportunité de recherche. En considerant les Méthodes d’Aide à la Décision, il y a des plusieurs type d’applications. L’objectif de la recherche c’est la proposition d’un modèle capable de sélectionner des processus pour faire l’audit dans une distributeur de carburant en considerant la prise de décision en groupe. La modélisation normative empirique c’est la méthode de recherche utilisée. Un modèle qui utilise l’Analytic Hierarchy Process (AHP) et la prise de décision en groupe a été proposé face aux difficultés d’un département d’Audit Interne. Le modèle proposé présente les poids des processus et permet leur priorisations dans un scénario determiné. Pour l’application de ce modèle présenté, on a utilisé cinq critères et 26 alternative (processus). L’information de jugement a été bien founi par trois spécialistes du département. Quelques rendez-vous avec les participants ont été pris pour obtenir et réviser les donnés ainsi que discuter les résultats. Les résultats obtenus par le modèle permettent d’identifier les processus qu’il faut plus d’attention. L’équipe a considéré le modèle comme une proposition d’aide à la prise de décision très pertinent pour le département d’Audit Interne

Análise funcional de um consórcio microbiano de solo e prospecção de genes envolvidos na desconstrução da biomassa

Pós-graduação em Microbiologia Agropecuária - FCAV

Diálogos entre Mário de Andrade e Francisco Curt Lange: nacionalismo e americanismo musicais nas décadas de 1930 e 1940

Pós-graduação em História - FCLAS

Área de vida e biologia reprodutiva da maria-da-restinga (Phylloscartes kronei) (Aves, Tyrannidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento da microbiota oral de bebês: análise longitudinal por pirosequenciamento da microbiota de bebês e suas mães dentro de um Programa de Saúde Bucal para Primeira Infância

Pós-graduação em Biopatologia Bucal - ICT

Tratamento da classe II hiperdivergente com o aparelho extra bucal de Thurow modificado

Objectives: natural dentofacial changes and that induced by the Thurow modified extra oral appliance (TMEOA) were evaluated in this prospective study. Methodology: the data consisted of fifteen Class II division 1 children 7 to 10 years old, with anterior open bite and hiperdivergent facial pattern treated with the Thurow appliance and of fifteen Class II division 1 children followed longitudinally from 6 to 12 years of age without treatment (Burlington Growth Centre, Toronto University, Canada). The analyses were based in traditional measurements obtained in lateral cephalometric radiographs scanned with the aid of the software Radiocef Studio®. Radiographs were taken in the beginning and after 1 year of treatment for the treated group and at the 6, 9 and 12 years of age for the control group. Results: the data analysis showed that the TMEOA significantly reduced the SNA, ANB, AOBO, SNPOc SNPM, SGO/NMe, OJ e OB. On the other hand the appliance did not interfere with the SNB e SNPP. The natural growth promoted significant change in the ANB, AOBO, SNPOc, OJ e OB from 6 to 9 years and in the SNB, SNPOc e SGo/NMe from 9 to 12 years. The restriction of the maxillary growth (SNA), reduction of the skeletal discrepancy (ANB) and the reduction of the overjet (OJ) were significant with the treatment considering the natural growth as verified in the control group. Conclusion: the TMEOA corrected the skeletal Class II malocclusion by maxillary restriction, reducing the overjet, closing the anterior open bite and decreasing both the hyper divergent facial pattern and mandible plane inclination.

Uso de um distrator ósseo dentoimplantossuportado, feito a partir de tornos expansores convencionais, na reabilitação oral

Osteodistraction is a clinical reality, available in the last decades for the resolution of large bone deficiencies, in cases that there are pre-existing, but misplaced implants and teeth. The aims of thearticle is to present a case report in which a new possibility for bone distraction, based on tooth-implant bone distractors, made from standard orthodontic expansion-screws, was used in an area where there was an extensive need of alveolar bone and aesthetical recovery, allied to teeth and dental implant misplacement. This technique presented good clinical results, associated to effective simplicity and low cost, becoming viable clinical solution for bone tissue augmentation and aesthetical optimization. In resume, it is possible to say that the suggested technique achieved its objectives, improving the hard and soft tissue profile, whilst allowing completion of oral rehabilitation.

Avaliação da capacidade de vedamento e pH do tampão apical com cimento Portland branco acrescido de radiopacificadores convencionais

The Portland cement has shown similar biological properties to calcium hydroxide, but its radiopacity is lower. Therefore, the addition of materials that minimize this deficiency should be considered. OBJECTIVE: To evaluate the apical sealing ability and pH of a white Portland cement added of several radiopacifying agents. MATERIAL AND METHODS: Forty bovine roots with incomplete apices were selected, and the foraminal openings were standardized to PM 720G bur. After the external root sealing, an apical intracanal barrier 10.0 mm thick was executed with the white Portland cement powder, pure or added of a radiopacifying agent (iodoform, zinc oxide or bismuth subnitrate), and distilled water (0.37 mL). The apical roots were immersed 24h in water in humid atmosphere, and after that they were immersed 24h in 2% Rhodamine B, under vacuum. In sequence, the roots were longitudinally sectioned, the root fragments were photographed, the images were digitalized and the apical infiltration was measured by the Image Tool program. The pH solutions were also evaluated, in 24h and 48h and 7 and 30 days. Data were submitted to Anova test. RESULTS: The zinc oxide solution has the lowest apical infiltration in relation to the other groups (p < 0.05). The pH behavior varied during the analysis, and in the period of 24h all groups showed the highest values (p < 0.05). CONCLUSION: The type of radiopacifying agent used interferes in the sealing ability of the apical barrier, and zinc oxide showed to be the most beneficial one. The pH varies according to the period of analysis, and the highest values were obtained in the first 24h (p < 0.05).

Avaliação do pH, da solubilidade e da infiltração marginal em retrobturações com o Sealer 26 puro ou acrescido de iodofórmio

Sealer 26® cement contains bisphenol epoxy resin associated with calcium hydroxide, presenting smaller radiopacity than other endodontic cements. Aiming to improve this property, iodoform has been added in its composition. However, this addition's possible changes in physical and chemical properties still need to be studied. OBJECTIVE: To evaluate the apical sealing ability, solubility, and pH of Sealer 26® alone or with iodoform, at several proportions. MATERIAL AND METHODS: Three experimental mixtures of Sealer 26®, alone or with iodoform, were prepared and subjected to solubility test. Additionally, these combinations were inserted into polyethylene tubes and immersed in distilled water, and, their pH was evaluated after 24-h and 7-day periods. Subsequently, forty roots of extracted lower incisors subdivided into four groups of 10 specimens each, were retrograde filled with one of the previously described mixtures and gutta-percha points. The roots were immersed in Rhodamine B, under vacuum, for 72 hours. After this period, the specimens were longitudinally sectioned, root fragments photographed, these images scanned, and apical infiltration measured by Image tool software. The obtained data were subjected to statistical analysis, at a significance level of 5%. RESULTS: Marginal leakage and solubility tests did not show any difference among the experimental groups (p > 0.05). pH analysis was only statistically different at 24-h period and between Sealer 26® alone and 1.1g iodoform group (p < 0.05). CONCLUSION: The presence of iodoform in Sealer 26®, at the used proportions, did not alter the solubility, apical marginal leakage and pH properties of the original cement.

Evaluación comparativa in vitro de la capacidad de sellado del sistema de conductos radiculares por diferentes técnicas

The aim of this study was to evaluate in vitro the sealing ability of the root canal using different techniques: cold lateral compaction, modified Hybrid Tagger, Thermafil and System B. Material and methods. Were used 116 human single-roots, included in resin blocks, sectioned longitudinally, attached to a device with screws and divided into four groups (n=29). After instrumentation using a bur, depression was made in each third of the root canal in each of the halves. Teeth were filled upon the different techniques. For evaluation of the quality, pictures were taken with a 1.5X magnification, photos and radiographs were utilized. After statistical analysis, were performed both macroscopic and radiographic evaluation, by Chi-square test (χ2). Results. The Thermafil technique showed better results in terms of sealing capacity, failures and homogeneity followed by modified Hybrid Tagger, the System B and cold lateral compaction. Conclusions. Thermafil was the technique that shown to be macroscopic, radiographic and homogeneously best over the other techniques evaluated in regard to the filling of the depressions made (AU)

Lesão endoperio: apicectomia com abordagem palatina: Relato de caso clínico com 30 meses de acompanhamento

Traditionally, the apicoectomies are carried out with the vestibular approach for visibility and easiness of access. The aim of this study was to present a case report of a patient with extensive injury endo-periodontal opted for the surgical access through a palatal incision. The patient presented with abscess drainage via periodontal ligament. The periodontal probing coincident with the radiographic apex in mesial tooth on dental element 22. Radiographically it was noted that the periapical lesion extended from the distal of the tooth 11 to the mesial of tooth 23. The tooth 22 had undergone endodontic treatment and showed signs of shutter material extravasation. It was decide to carry out an approach by the Palatine of the tooth to prevent gingival. After the flap elevation, the injury was debrided and apicoectomy was performed. The patient reported no pain or discomfort after surgery. Furthermore, as follow-up of 30 months there was total remission of signs and symptoms presented initially and absence of gingival recession. Therefore, according to the results showed in this case report, it is suggested that the Palatine access is an alternative approach that can be successfully employed in cases of apicoectomies in order to avoid the occurrence of gingival recessions.