Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos

Analysis of the products of oxidative degradation of Hb S was made by methahemoglobin measurement and a count of red blood cells with Heinz bodies. Free radicals originating from oxidation cause extensive injury to erythrocytes, decreasing their useful survival period especially in Hb S carriers. The Superoxide ion (O 2) is the most responsible for the oxidation process of Hb forming membrane-bound haemachromes which afterwards evolve to Heinz bodies, damaging the membrane and provoking erythrocytes hemolysis. The results from this work showed that the SS genotype is more susceptible to the action of the free radicals than the S/Tal genotype. The β genotype has a lower oxidative susceptibility than the SS because it has only one β s mutation. The results allowed us to conclude that: a) the simple presence of Hb S, independent of its genotype and its concentration, is sufficient to produce methaemoglobin from this Hb; b) there is not a direct relationship between methaetnoglobin concentration and the Heinz bodies count; c) the intensity of Heinz bodies in the sickled erythrocytes seems to be independent of the Hb Fetal concentration; d) The genotype SS is more susceptible to Hb oxidation with the release of products of oxidative degradation; e) methaemoglobin formation in blood of people with Hb AA and Hb AS, assessed over 24, 48, 72 and 163 hourly-periods, showed greater oxidative intensity in the Hb AS compared with Hb AA.

Pré-oxidação e adsorção em carvão ativado granular para remoção dos herbicidas Diuron e Hexazinona de água subterrânea

The cultivation of sugarcane demands the use of herbicides such as Diuron and Hexazinone. Some supply wells from Ribeirão Preto, SP, Brazil, built in the Guarani Aquifer are located in recharge points, and the presence of sandy Quartzarenic Neosol in these areas increases the vulnerability of the groundwater to contamination from herbicides This paper reports the water quality monitored in some wells located in the recharge area and the removal of Diuron and Hexazinone by means of adsorption in granular activated carbon (GAC), preceded or not by preoxidation with chlorine and chlorine dioxide in a pilot plant. The results indicated that Diuron was more strongly adsorbed than Hexazinone and that the saturation time of the GAC in the test with preoxidation was shorter than in the test without preoxidation, which may have occurred mainly due to the formation of by-products that competed with the adsorption of the herbicides.

Degradação ruminal e síntese de proteína microbiana em bovines alimentados com silagem de sorgo contendo tanino suplementado com concentrado ou uréia

This work aimed to determine ruminal parameters of dry mater (DM), neutral detergent fiber (NDF) and protein synthesis in the rumen of animals fed diets based on sorghum with different tannin levels. In situ degradation was evaluated by rumen incubation of level 1 (L1) and level 2 (L2) sorghum silages during 0, 6, 24, 48 and 96 hours. A duplicated 4 × 4 Latin Square was used. Potential degradability (PD) of DM was similar among silages, whereas the effective degradability (ED) decreased when the diets were supplemented with concentrate. Microbial nitrogen flux and microbial synthesis efficiency were not affected by roughage source. The synthesis efficiency, expressed in organic matter and crude protein digested in rumen, was higher in L1 tannin diets supplemented with concentrate. There was not relationship between the presence of tannins and the parameters of ruminal degradation.

Influência de laminas de chuva no controle de plantas daninhas, semeadas em diferentes profundidades no solo, com aplicação de herbicidas pré-emergentes

The aim of this work was to evaluate the control in pre-emergence of the weeds brachiaria grass (Brachiaria decumbens) and goosegrass (Eleusine indica) in different depths in the ground, for the oxyfluorfen and isoxaflutole herbicides, applied in pre-emergence condition and submitted of different rain blades. The experimental design was entirely at random, with four repetitions. Each set was consisted of plastic vases with substratum capacity of 4L, filled with arenaceous ground, increased of brachiaria grass and goosegrass seeds in the depths: 0,5; 1,0; 3,0; 6,0 and 9,0 cm. Doses of 37,5 g a.i. ha -1 of isoxaflutole and 720 g a.i. ha -1 of oxyfluorfen were used. The rain blades was 5, 10 and 20 mm applied with a stationary rain simulator, beyond a treatment without rain. Visual evaluations of control at 7 and 14 days had been carried through after application of the herbicides, counting of germinated plants and dry biomass of plants without application. The development of both weeds was inhibited by herbicides, reaching 100% of control, in all depth levels. The different rain blades had not influenced the control of the weeds. In the biggest depth of sowing (9,0 cm), the emergence was above 45% to B. decumbens and 36% to E. indica in treatments without herbicides application.

Sistema especialista para a monitoração do processo de degradação de medidores tipo indução de energia elétrica ativa

This paper presents a methodology and software for hazard rate analysis of induction type watt-hour meters, considering the main variables related with the degradation process of these meters, for the Elektro Electricity and Services SA. The modeling developed to calculate the watt-hour meters hazard rate was implemented in a tool through a user friendly platform, in Delphi language, enabling not only hazard rate analysis, but also a classification by risk range, localization of installation for the analyzed meters, and, allowing, through an expert system, the sampling of induction type watt-hour meters, based on the model risk developed with artificial intelligence, with the mainly goal of follow and manage the process of degradation, maintenance and replacement of these meters. © 2010 IEEE.

Tolerância de Espécies de Mucuna a Herbicidas Utilizados na Cultura da Cana-de-Açúcar

The emergence of Mucuna species in sugarcane plantations, even after herbicide spraying for weed management, allowed us to elaborate the hypothesis that these plants are tolerant to the herbicides commonly used in the culture. Thus, to prove this hypothesis, this work was carried out to study the tolerance of Mucuna aterrima, Mucuna cinerea and Mucuna deeringiana to application of herbicides with different mechanisms of action in pre- and postemergence. The experiment was arranged in a randomized block design with 9 treatments and five replications in a factorial 3 × 3, plus controls. At pre-emergence, the first factor was constituted by the herbicides sulfentrazone (800 g ha-1), imazapic (245 g ha-1), and amicarbazone (1,400 g ha-1), and the second factor by the Mucuna species, besides the additional control for each species studied. At post- emergence, the herbicides tested were clomazone (1,100 g ha-1), ametryn+trifloxysulfuronsodium (1,463 + 37 g ha-1), and 2,4-D (1,209 g ha-1). In the chemical management at pre-emergence, the species were sensitive to amicarbazone, followed by sulfentrazone, and tolerant to imazapic. At post-emergence, all species were sensitive to ametryn + trifloxysulfuron-sodium and 2,4-D, but tolerant to clomazone.

Déficit hídrico na eficiência de herbicidas e nas características bioquímicas de Euphorbia heterophylla

The objective of this work was to evaluate conditions the effectiveness of acetolactate synthase (ALS) and protoporphyrinogen oxidase (PROTOX) inhibitors in the Bidens pilosa control under two water deficit conditions, as well as to determine the action under the content of soluble carbohydrates and protein and free amino acids of weed. The experimental design was randomized completely design, with four replications, with the treatments setup in a factorial scheme 4x2, with four herbicides (fomesafen lactofen, chlorimuron-ethyl and imazethapyr), and two soil water conditions (-0.5 MPa and -0.01MPa). At 7, 14, 21 and 28 days after application (DAA), was assessed visually control efficiency of herbicides. For the determination of organic solutes plants were collected at 24, 48, 72 and 96 hours after application (HAA), except for the amino acids were analyzed 48, 72 e 96 HAA. Herbicides fomesafen and lactofen were efficient to control E. heterophylla, while the ALS inhibitors (chlorimuron-ethyl e imazethapyr) provided an unsatisfactory control. Water deficit altered the efficiency of herbicides, mainly chlorimuronethyl. Lactofen provided a smaller content of soluble carbohydrates, in the same way, the protein ranged in the 72 HAA, the lower value observed for imazethapyr e lactofen respectively. Herbicide lactofen increased the concentration of free amino acids, while the imposition of water deficit caused an increase in soluble carbohydrate content.

Emergência de Rottboellia exaltata influenciada pela profundidade de semeadura, quantidade de palha de cana sobre o solo e aplicação de herbicidas residuais

Mechanical sugarcane harvest without burning and continuous straw on the soil surface may affect the Rottboellia exaltata infestation dynamics in sugarcane fields. Three greenhouse experiments were conducted with the aim of studying the effects of sowing depth (0, 2.5, 5, 7.5, and 10 cm), amount of sugarcane straw on the soil surface (0, 5, 10, and 15 ton ha-1), and residual herbicide (clomazone, flumioxazin, imazapyr, isoxaflutole, and s-metolachlor) on the emergence of Rottboellia exaltata. For each experiment, a completely randomized design with four replicates was applied. The combination of mulch on soil surface (especially with larger amounts of straw) with deeper sowing depths provides less emergence and mass accumulation of R. exaltata. In bare soil, the sowing depth did not affect the weed dynamics. Clomazone and imazapyr were effective herbicides controlling R. exaltata regardless of the amount of straw on the soil surface. Flumioxazin was also effective in controlling R. exaltata but only under bare soil conditions. Even with 60 mm of accumulated rainfall over the 4 day period after application, the amount of flumioxazin leached to the soil was not enough to ensure the same control observed when applying the herbicide on bare soil.

Respostas fisiológicas de cultivares de cana-de-açúcar a herbicidas seletivos

The use of selective herbicides to control weeds has caused different responses in cultivars of sugar cane, and some products affect physiological characteristics and reduce the photosynthetic activity. This study aimed to evaluate the physiological traits in cultivars of sugar cane under the effect of applying post-emergence herbicides. The test was developed in Jau, SP. The experimental design was randomized blocks in factorial scheme 5 x 4 (cultivar x herbicide) with four replications. SP81-3250, RB855156, RB855453, RB867515, IACSP95-5000 were grown in this studied. Herbicides clomazone (1200 g i.a.ha-1); commercial mixture of clomazone + ametryn (1000 + 1500 g i.a.ha-1) and ametryn (3000 g i.a.ha-1) and a control were applied at 30 days after planting. Cultivars IACSP95-5000 and RB867515 were less affected physiologically and can be considered selective to these herbicides. The application of clomazone and ametryn affected negatively the traits maximum photochemical efficiency of photosystem II (Fv/Fm), SPAD index and photosynthetic pigments, but the mixture of these herbicides caused higher reductions, indicating to be the more aggressive to the cultivars.

Eficiência de herbicidas inibidores da ALS e PROTOX sob condições de déficit hídrico no comportamento bioquímico de plantas daninhas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Intervalos de chuva na eficiência de herbicidas aplicados em pós-emergência

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Seletividade de herbicidas à cultura da mandioca

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Tolerância de Digitaria ciliaris, Digitaria horizontalis e Digitaria nuda à herbicidas usados em cana-de-açúcar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo da lixiviação de herbicidas utilizados na cultura da cana-de-açúcar com plantas bioindicadoras

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Seletividade de herbicidas aplicados em pré-emergência e associados à nematicidas, à cultura da cana-de-açúcar cultivar BR855113

Pós-graduação em Agronomia (Agricultura) - FCA