Leishmaniose Visceral canina: Clonagem e expressão das proteínas Lc24 e Lc36 de L. chagasi com potencial aplicação no diagnóstico e desenvolvimento de vacina

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

TLR 2, TLR 4, ROS, óxido nítrico, P38 e IKK em células mononucleares de cães com leishmaniose Visceral tratadas com o imunomodulador P-MAPA: Larissa Martins Melo. -

Pós-graduação em Ciência Animal - FMVA

Relação entre carga parasitária de cães naturalmente acometidos por leishmaniose visceral e infectividade para Lutzomyia longipalpis: Lilian Aparecida Colebrusco Rodas. -

Pós-graduação em Ciência Animal - FMVA

Relação entre presença de anticorpos anti-leishmania spp. e carga parasitária em sangue de cães de área endêmica para leishmaniose visceral

Pós-graduação em Ciência Animal - FMVA

Imunofenótipos de linfócitos T no sangue e no liquor de cães com leishmaniose visceral: correlação com as lesões encefálicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Infecção de Lutzomyia longipalpis por leishmania spp. em área urbana endêmica para leishmaniose visceral no Estado de São Paulo, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação das lesões encefálicas e fenotipagem de linfócitos T e células dentríticas em linfócitos de cães com leishmaniose visceral

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Serological detection of Toxoplasma gondii, Leishmania infantum and Neospora caninum in cats from an area endemic for leishmaniasis in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Orientação sobre posse responsável em uma área endêmica para leishmaniose visceral canina

The purpose of this work was to provide guidance to householders in the responsible care of their pets, in a region where canine visceral leishmaniasis is endemic. Questionnaires on responsible ownership and the control of this disease were completed by owners of dogs and cats in the city of Araçatuba, São Paulo State, Brazil, in order to evaluate their level of knowledge. Based on the identified deficiencies, the project team provided the owners of the pets with individual guidance at home, focusing on the most critical issues. Concerning nutrition, 70.45% (62/88) of owners provided pet food only, 27.28% (24/88) administered a mixed diet, and 2.27% (2/88) provided homemade food. Additionally, 78.41% (69/88) did not permit the consumption of raw meat, rare meat, or bones. 9.09% (8/88) of owners allowed their pets to wander the neighborhood alone. Among dogs, 14.77% (13/88) had never been clinically tested by a qualified professional, and 6.82% (6/88) and 15.91% (14/88) had never received any kind of vaccine or vermifuge, respectively. In relation to population control, only 20.45% (18/88) of the dogs had been sterilized. It was also observed that 25% (22/88) of owners had already had canine visceral leishmaniasis seropositive dogs in their homes. 54.55% (12/22) of these owners had referred their pets to veterinary clinics for euthanasia, 22.73% (5/22) had used the services of the city’s animal disease control center, and 18.18% (4/22) had paid for private treatment. Nevertheless, 35.23% (31/88) of the dogs had never been submitted to tests for infection diagnosis. From the results obtained, it could be inferred that the individuals who participated in this study were poorly informed concerning basic pet care, demonstrating a need to implement community awareness campaigns for the responsible ownership of pets.

Ações educativas para o controle de vetores da dengue e leishmaniose visceral

Results of educative actions on the control of vectors for dengue and leishmaniasis were evaluated by using an online semi-present course directed to teachers from Fundamental I Education level in Araçatuba-SP. 40 teachers from municipal schools located on geographical areas of town with higher occurrence of dengue and leishmaniasis cases, attended to the course, which consisted in a conceptual part directed to specific subjects such as vector borne and zoonotic diseases, responsible ownership of pets and health education and a practical phase, directed to execution and application of educative projects in the target schools. Data about the teacher’s specific knowledge, before and after the attendance to the course, were submitted to the Wilcoxon test with a 5% of significant level. The application of practical educative projects was evaluated by multiplicative actions involving target public. Tests showed statistically significant increase in the knowledge about the studied diseases (p<0.0001) after the course, especially on questions related to etiological agent, clinical symptoms on man and animals and prevention. Activities of knowledge multiplication envolved teachers and students which detected the problems and promoted education actions in their neighborhood as stage performing, group cleaning activities and elaboration and distribution of folders during a student pared. In conclusion, educative actions direct to the control of diseases vectors using a semi-present course, provides new knowledge to the teachers, impelling them to motivate their students to adopt vectors control measures and environmental cares and to sensitive the community to collaborate with control diseases.

Clinical and epidemiological aspects of feline leishmaniasis in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atividades de educação em saúde sobre leishmaniose visceral para escolares

This study aimed at evaluating the knowledge on visceral leishmaniasis gained after the application of an educative project for the 6th and 7th grade students from three public schools of Birigui, SP, Brazil. A questionnaire before (Phase I) and after (Phase II) activities that comprehended one conference by a health agent, a comic contest and one crossword about VL was used to measure scholar’s knowledge. We interviewed 711 students in Phase I and 693 in Phase II. A criterion of VL knowledge was adopted as “Good”, “Medium” and “Bad” when, out of 10 questions analyzed by Item Response Theory, 10 to 8, 7 to 4, and 3 to 0 were right, respectively. We observed a statistically significant increase in the students’ knowledge level after the educational project, since the number of students with “Good” concept changed from 35.7% (Phase I) to 59.7% (Phase II). The educational activities carried out led to gains in knowledge among students suggesting that continuing education can bring good results to public health.

Leishmaniose visceral no Brasil

Visceral leishmaniasis (VL) is among the most important vector-borne diseases that occur in Brazil, mainly due to its zoonotic nature. It is currently present in almost all Brazilian territory, and its control is a challenge both for veterinarians and for public health officials. The etiologic agent is Leishmania infantum (syn chagasi), and the main vector in Brazil is Lutzomyia longipalpis. Of all animals identified as reservoirs of VL, the dog is considered the most important domestic reservoir. Although the disease has already been identified in cats, the epidemiological role of this animal species is still unclear. This article presents a brief review of the epidemiological situation of the disease, its mode of transmission, clinical features in dogs and cats as well as possible risk factors associated with the occurrence of the disease in Brazil.

Leucograma e metabolismo oxidativo dos neutrófilos de cães com leishmaniose visceral antes e após o tratamento com Antimoniato de Meglumina e Alopurinol

Leukogram and neutrophil oxidative metabolism from dogs with visceral leishmaniasis (VL) were compared before and after treatment with meglumine antimoniate (AM) and with an association of meglumine antimoniate and allopurinol (AMA). The results obtained demonstrated that neutrophils of dogs with VL do not lose their capacity of reducing NBT and that oxidative metabolism has been more active in the majority of the cases. After treatment with AM and AMA, dogs with VL presented a redution of neutrophils oxidative metabolism, suggesting that this decrease was related with the decrease in the number of parasites and a probable inhibitor effect of these drugs on neutrophils oxidative metabolism.

Diagnóstico de situação epidemiológica da Leishmaniose visceral canina em Rio Verde-GO

Pós-graduação em Medicina Veterinária - FCAV