Crystallization, preliminary X-ray analysis and molecular-replacement solution of the carboxy form of haemoglobin I from the fish Brycon cephalus

Haemoglobin, the 'honorary enzyme' [Brunori (1999), Trends Biochem. Sci. 24, 158-161], constitutes a prime prototype for allosteric models. Here, the crystallization and preliminary X-ray analysis of haemoglobin I from the South American fish Brycon cephalus are reported. X-ray diffraction data have been collected to 2.5 Angstrom resolution using synchrotron radiation (LNLS). Crystals were determined to belong to the space group P6(1)22 and preliminary structural analysis revealed the presence of one dimer (alpha beta) in the asymmetric unit. The structure was determined using standard molecular-replacement techniques.

Chemical, spectroscopic characterization, and in vitro antibacterial studies of a new gold(I) complex with N-acetyl-L-cysteine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

IFPA Meeting 2010 Workshop Report I: Immunology; Ion transport; Epigenetics; Vascular reactivity; Epitheliochorial placentation; Proteomics

Workshops are an important part of the IFPA annual meeting. At IFPA Meeting 2010 there were twelve themed workshops, six of which are summarized in this report 1. The immunology workshop focused on normal and pathological functions of the maternal immune system in pregnancy. 2. The transport workshop dealt with regulation of ion and water transport across the syncytiotrophoblast of human placenta. 3. The epigenetics workshop covered DNA methylation and its potential role in regulating gene expression in placental development and disease. 4. The vascular reactivity workshop concentrated on methodological approaches used to study placental vascular function. 5. The workshop on epitheliochorial placentation covered current advances from in vivo and in vitro studies of different domestic species. 6. The proteomics workshop focused on a variety of techniques and procedures necessary for proteomic analysis and how they may be implemented for placental research. (C) 2011 Published by IFPA and Elsevier Ltd.

Epidemiology and genetics of endemic goiter. I. Epidemiological aspects

This study dealt with approximately 2,000 children and their parents living in a hyperendemic goiter area in Central Brazil, which is bounded by the jungle to the north and by a large plain to the south. The determination of goiter was made according to the methods and classification adopted by WHO. Conspicuous forms of goiter were found in 41% of the children examined. Multiple linear regression analysis showed an increase with age in the frequency of goiter in both sexes. Although data from the literature show that Mulattoes and Negroes have statistically higher frequencies of goiter than do Whites, our multiple linear regression analysis revealed no evidence for an effect of race on the endemism. There was no significant association with socioeconomic level. The presence of goiter in parents was shown to be statistically associated with its occurrence in the children.

Serology of paracoccidioidomycosis - I. Evaluation of the indirect immunofluorescent test

The purposes of the present work were: i) to study the positivity indices and compare titers obtained with the indirect immunofluorescence (II), tube precipitation (TP), complement fixation (CF) and double immunodiffusion on agar gel (ID) tests in the sera of 196 patients with paracoccidioidomycosis before treatment, and ii) to compare the initial titers of II with those obtained 1 year or more after treatment. II was the most sensitive serologic reaction (85.2%), and the positivity indices for CF, ID and TP were 67.7%, 66.0% and 50.0%, respectively. The sera tended to show parallel mean titers in II, CF and TP tests. One year after treatment there was a fall in titers of II in 66.2% of patients. The data, taken as a whole, demonstrate the usefulness of the indirect immunofluorescent test and the importance of using 2 or more serologic tests for the diagnosis and monitoring of patients with paracoccidioidomycosis. © 1985 Martinus Nijhoff/Dr W. Junk Publishers.

Crystallization and X-ray diffraction data analysis of oxyhemoglobin-I from the catfish Liposarcus anisitsi (pisces)

Hemoglobin remains, despite the enormous amount of research involving this molecule, as a prototype for allosteric models and new conformations. Functional studies carried out on Hemoglobin-I from the South-American Catfish Liposarcus anisitsi [1] suggest the existence of conformational states beyond those already described for human hemoglobin, which could be confirmed crystallographically. The present work represents the initial steps towards that goal.

Application of C-banding in two Arachis wild species, Arachis Pintoi Krapov. and W.C. Gregory and A. villosulicarpa Hoehne to mitotic chromosome analyses

Two wild diploid (2n = 20 chromosomes) and self-pollinating Arachis species, Arachis Pintoi Krapov and W.C. Gregory and A. villosulicarpa Hoehne were submmited to C-band technique to karyotype analyses. Root tips were employed in the analyses. Morphometric data chose that chromosome lengths varied from 3.12 in A. villosulicarpa to 1.45 in A. Pintoi. Karyotype formula obtained was 10sm to A. Pintoi and 9sm + 1m to A. villosulicarpa. There was a predominance of pericentromeric C-band in all mitotic metaphasic chromosomes in both species. Besides C-band values, both species still did not differ in respect to chromosome absolute and relative lengths, centromeric index, symmetry index and total karyotype haploid length. C-band and morphometric data did not show strong or significant differences which could separate these two species of peanut which belong to evolutive different sections.

Measurement of the pp(-)-> W gamma plus X cross section at root s=1.96 TeV and WW gamma anomalous coupling limits

The WWγ triple gauge boson coupling parameters are studied using pp̄rarr; νγ+X(=e,μ) events at s=1.96 TeV. The data were collected with the D0 detector from an integrated luminosity of 162pb-1 delivered by the Fermilab Tevatron Collider. The cross section times branching fraction for pp̄→W(γ)+X→ νγ+X with ETγ>8 GeV and ΔR γ> 0.7 is 14.8±1.6(stat)±1.0(syst) ±1.0(lum)pb. The one-dimensional 95% confidence level limits on anomalous couplings are -0.88<Δκγ<0.96 and -0. 20<λγ<0.20. © 2005 The American Physical Society.

First study of the radiation-amplitude zero in W gamma production and limits on anomalous WW gamma couplings at root s=1.96 TeV

We present results from a study of pp̄→Wγ+X events utilizing data corresponding to 0.7fb-1 of integrated luminosity at s=1.96TeV collected by the D0 detector at the Fermilab Tevatron Collider. We set limits on anomalous WWγ couplings at the 95% C.L. The one-dimensional 95% C.L. limits are 0.49<κγ<1.51 and -0.12<λγ<0.13. We make the first study of the charge-signed rapidity difference between the lepton and the photon and find it to be indicative of the standard model radiation-amplitude zero in the Wγ system. © 2008 The American Physical Society.

Search for W H associated production in 5.3 fb(-1) of p(p)over-bar collisions at the Fermilab Tevatron

We present a search for associated production of Higgs and W bosons in pp̄ collisions at a center of mass energy of s=1.96 TeV in 5.3 fb-1 of integrated luminosity recorded by the D0 experiment. Multivariate analysis techniques are applied to events containing one lepton, an imbalance in transverse energy, and one or two b-tagged jets to discriminate a potential WH signal from Standard Model backgrounds. We observe good agreement between data and expected backgrounds, and set an upper limit of 4.5 (at 95% confidence level and for mH=115 GeV) on the ratio of the WH cross section multiplied by the branching fraction of H→bb̄ to its Standard Model prediction, which is consistent with an expected limit of 4.8. © 2011 Elsevier B.V.

Measurements of inclusive W and Z cross sections in pp collisions at √s = 7 TeV The CMS collaboration

Measurements of inclusive W and Z boson production cross sections in pp collisions at √s = 7 TeV are presented, based on 2.9 pb-1 of data recorded by the CMS detector at the LHC. The measurements, performed in the electron and muon decay channels, are combined to give σ(pp → WX) × B(W → l?) = 9.95 ± 0.07 (stat.) ± 0.28 (syst.) ± 1.09 (lumi.) nb and σ(pp → ZX) × B(Z → l +l-) = 0.931 ± 0.026 (stat.) ± 0.023 (syst.) ± 0.102 (lumi.) nb, where l stands for either e or μ. Theoretical predictions, calculated at the next-to-next-to-leading order in QCD using recent parton distribution functions, are in agreement with the measured cross sections. Ratios of cross sections, which incur an experimental systematic uncertainty of less than 4%, are also reported.

Measurement of the lepton charge asymmetry in inclusive W production in pp collisions at √s = 7 TeV

A measurement of the lepton charge asymmetry in inclusive pp → WX production at √s = 7TeV is presented based on data recorded by the CMS detector at the LHC and corresponding to an integrated luminosity of 36 pb -1. This high precision measurement of the lepton charge asymmetry, performed in both the W → eν and W → μν channels, provides new insights into parton distribution functions. Copyright CERN.

Measurement of the W boson mass with the D0 detector

We present a measurement of the W boson mass using data corresponding to 4.3fb -1 of integrated luminosity collected with the D0 detector during Run II at the Fermilab Tevatron pp̄ collider. With a sample of 1677394 W→eν candidate events, we measure M W=80.367±0. 026GeV. This result is combined with an earlier D0 result determined using an independent Run II data sample, corresponding to 1fb -1 of integrated luminosity, to yield M W=80.375±0.023GeV. © 2012 American Physical Society.

Combination of CDF and D0 measurements of the W boson helicity in top quark decays

We report the combination of recent measurements of the helicity of the W boson from top quark decay by the CDF and D0 collaborations, based on data samples corresponding to integrated luminosities of 2.7-5.4fb -1 of pp̄ collisions collected during Run II of the Fermilab Tevatron collider. Combining measurements that simultaneously determine the fractions of W bosons with longitudinal (f 0) and right-handed (f +) helicities, we find f 0=0.722±0.081[±0.062(stat)±0.052(syst)] and f +=-0.033±0.046[±0.034(stat)±0.031(syst)]. Combining measurements where one of the helicity fractions is fixed to the value expected in the standard model, we find f 0=0.682±0. 057[±0.035(stat)±0.046(syst)] for fixed f + and f +=-0.015±0.035[±0.018(stat)±0.030(syst)] for fixed f 0. The results are consistent with standard model expectations. © 2012 American Physical Society.