167 resultados para TRYPANOTHIONE REDUCTASE


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Ciência e Tecnologia Animal - FEIS

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The use of biostimulants can alter plant growth and development, but the action of they may be varied according to the stage of development of the plant. The aim was to evaluate the effects of forms and times of a biostimulant (cytokinin, indolebutyric acid, and gibberellic acid) application on nodulation, some biochemical aspects, growth and yield of common bean cultivar Perola. The treatments were: Control (without application); TS - 250 mL ha(-1) seed treatment; V-4 - 250 mL ha(-1) foliar spray in V-4 stage; R-5 - 250 mL ha(-1) foliar spray in R-5 stage; TS+V-4 - 250 mL ha(-1) in TS + 250 mL ha(-1) in V-4; TS+R-5 - 250 mL ha(-1) in TS + 250 mL ha(-1) in R-5; V-4+R-5 - 250 mL ha(-1) in V-4 + 250 mL ha(-1) in R-5, and TS+V-4+R-5 - 250 mL ha(-1) in TS + 250 mL ha(-1) in V-4 + 250 mL ha(-1) in R-5. The foliar biostimulant application in the vegetative (V-4) or early reproductive phase (R-5) increases nodulation, root growth, content of soluble sugars, content of total amino acid and nitrate reductase activity, however, does not interfere with shoot growth and grain yield of common bean.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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This study aimed to assess antioxidant effects of melatonintreatment compared to N-acetylcysteine (NAC) and to their combination in asickle cell suspension. Sickle erythrocytes were suspended in phosphate-buffered saline, pH 7.4, composing external control group. They were alsosuspended and incubated at 37°C either in the absence (experimental controlgroup) or in the presence of NAC, melatonin and their combination atconcentrations of 100 pM, 100 nM and 100 lM for 1 hr (treatment groups).The melatonin influences were evaluated by spectrophotometric [hemolysisdegree, catalase (CAT), glutathione S-transferase (GST), glutathioneperoxidase (GPx), glutathione reductase (GR), glucose-6-phosphatedehydrogenase (G6PDH), and superoxide dismutase (SOD) activities] andchromatographic methods [glutathione (GSH) and malondialdehyde (MDA)levels]. Incubation period was able to cause a rise about 64% on hemolysisdegree as well as practically doubled the lipid peroxidation levels (P < 0.01).However, almost all antioxidants tested treatments neutralized this incubationeffect observed in MDA levels. Among the antioxidant biomarkers evaluated,we observed a modulating effect of combined treatment on GPx and SODactivities (P < 0.01), which showed ~25% decrease in their activities. Inaddition, we found an antioxidant dose-dependent effect for melatonin onlipid peroxidation (r = 0.29; P = 0.03) and for combined antioxidanttreatments also on MDA levels (r = 0.37; P = 0.01) and on SOD activity(r = 0.54; P < 0.01). Hence, these findings contribute with important insightthat melatonin individually or in combination with NAC may be useful forsickle cell anemia management.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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