Marginal adaptation of Class V restorations with current generation dentin-bonding agents: effect of different dentin surface treatments.

This study sought to assess the use of chlorhexidine with several excipients as a dentin surface treatment and its effect on marginal adaptation of class V restorations with current-generation dentin bonding agents. A total of 120 human third molars were selected and allocated into 12 groups, with standardized buccal class V restorations randomly divided into preconditioned dentin rinsed with: water; water + chlorhexidine; ethanol; or ethanol + chlorhexidine. After rinsing of dentin (previously conditioned with 35% phosphoric acid) with the test solutions, the Adper single bond 2, prime and bond 2.1, and Excite bonding systems were applied randomly. Restorations were performed with FiltekTM Z350 XT composite resin. The resulting specimens were subjected to thermal and mechanical load cycling. Quantitative analysis of marginal adaptation was performed on epoxy replicas by means of scanning electron microscopy. Results were assessed by means of the Kruskal-Wallis test (percentages of continuous margins) and Wilcoxon test (differences between percentages of continuous margins before and after thermal cycling and mechanical loading), at a significance level of p < 0.05. Outcomes in the chlorhexidine-treated groups were not superior to those obtained with other treatments.

MicroRNA 21 is a homeostatic regulator of macrophage polarization and prevents prostaglandin E-2-mediated M2 generation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Frequency tunable continuous THz wave generation in a periodically poled fiber

An all-fiber approach to terahertz generation using a periodically poled optical fiber is proposed and experimentally demonstrated. In the proposed approach, a continuous-wave THz wave is generated at a periodically poled fiber by beating two optical wavelengths from two laser sources with the wavelength spacing corresponding to the frequency of the THz wave. The key component in the system is the periodically poled fiber, which is made by a twin-hole fiber with the fiber core residing between two holes. The twin-hole fiber is then thermally poled at a temperature of similar to 260 degrees C with a voltage of 3.3 kV applied to the silver electrodes inside the two holes to introduce second-order nonlinearity. The quasi phase matching (QPM) condition is achieved by periodically erasing the thermal poling induced second-order nonlinearity with an ultraviolet laser, which enhances the energy conversion efficiency. The proposed approach is validated by an experiment. The emission of a THz wave centered at 3.8 THz with an output power of 0.5 mu W is observed. The frequency tunability between 2.2 and 3.8 THz is also experimentally demonstrated.

Energetic exploitation from a hybrid PV-wind power micro-generation rural electrification

Hybrid system micro-generation integration of PV-wind power is presented by a form of energy in which problems resulting from variability in the intensity of wind and solar intensity are possible mitigation either by complementation between one source to another or the largest stability configured by the generate the system. Based on this context, this work aims to assessing the performance of a hybrid system PV-wind power energy small of a rural property for their electrification. The study has been developed at the Rural Laboratory Powering from Engineering Department of UNESP. In order to present this research, a hybrid system has been installed PV-wind power, composed of one 400Wp windmill and a 300 Wp PV-system. The results obtained allowed us to evaluate the solar and wind energy supplied ranked among 285 and 360 kWh electric power generated by the PV-wind power hybrid system stood between 25,5 and 31 kWh. At is to say achieving yield of approximately than 10% during one year observation period, i.e., it was concluded that the performance of the hybrid system depended essentially the energy received and generated by the PV-system and that there was complementation between generating wind power and PV-systems with regard to time of day and the annual seasons by confirming the technical feasibility of this kind system of micro-generation in small rural properties.

Anomalous couplings of the third generation in rare B decays

We study the potential effects of anomalous couplings of the third generation quarks to gauge bosons in rare B decays. We focus on the constraints from flavor changing neutral current processes such as b→sγ and b →sl+l-. We consider both dimension-four and dimension-five operators and show that the latter can give large deviations from the standard model in the still unobserved dilepton modes, even after the bounds from b→sγ and precision electroweak observables are taken into account. ©2000 The American Physical Society.

HLA-E coding and 3' untranslated region variability determined by next-generation sequencing in two West-African population samples

HLA-E is a non-classical Human Leucocyte Antigen class I gene with immunomodulatory properties. Whereas HLA-E expression usually occurs at low levels, it is widely distributed amongst human tissues, has the ability to bind self and non-self antigens and to interact with NK cells and T lymphocytes, being important for immunosurveillance and also for fighting against infections. HLA-E is usually the most conserved locus among all class I genes. However, most of the previous studies evaluating HLA-E variability sequenced only a few exons or genotyped known polymorphisms. Here we report a strategy to evaluate HLA-E variability by next-generation sequencing (NGS) that might be used to other HLA loci and present the HLA-E haplotype diversity considering the segment encoding the entire HLA-E mRNA (including 5'UTR, introns and the 3'UTR) in two African population samples, Susu from Guinea-Conakry and Lobi from Burkina Faso. Our results indicate that (a) the HLA-E gene is indeed conserved, encoding mainly two different protein molecules; (b) Africans do present several unknown HLA-E alleles presenting synonymous mutations; (c) the HLA-E 3'UTR is quite polymorphic and (d) haplotypes in the HLA-E 3'UTR are in close association with HLA-E coding alleles. NGS has proved to be an important tool on data generation for future studies evaluating variability in non-classical MHC genes.

Neutrophil extracellular traps identification in tegumentary lesions of patients with paracoccidioidomycosis and different patterns of NETs generation in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

New fermions and a vectorlike third generation in SU(3)(C)circle times SU(3)(L)circle times U(1)(N) models

We study two 3-3-1 models with (i) five (four) charge 2/3 (-1/3) quarks and (ii) four (five) charge 2/3 (-1/3) quarks and a vectorlike third generation. Possibilities beyond these models are also briefly considered.

Multifunctional current reference generation strategy for grid-tied power electronic converter

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)